scholarly journals A Comparative Study of Static Biofilm Formation and Antibiotic Resistant Pattern between Environmental and Clinical Isolate of <i>Pseudomonas aeruginosa</i>

2020 ◽  
Vol 10 (12) ◽  
pp. 663-672
Author(s):  
Fahareen Binta Mosharraf ◽  
Sara Sadia Chowdhury ◽  
Akash Ahmed ◽  
M. Mahboob Hossain
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Comparative Study ◽  
Clinical Isolate ◽  
Biofilm Formation ◽  
Antibiotic Resistant

scholarly journals Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 in Pseudomonas aeruginosa

2018 ◽  
Author(s):  
Prasanth Manohar ◽  
Thamaraiselvan Shanthini ◽  
Reethu Ann Philip ◽  
Subramani Ramkumar ◽  
Manali Kale ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Biofilm Formation ◽  
Tamil Nadu ◽  
Antibiotic Resistance Genes ◽  
Clinical Samples ◽  
Cross Sectional ◽  
Antibiotic Resistant ◽  
Resistant Genes

AbstractTo evaluate the presence of biofilm-specific antibiotic-resistant genes, PA0756-0757, PA5033 and PA2070 in Pseudomonas aeruginosa isolated from clinical samples in Tamil Nadu. For this cross-sectional study, 24 clinical isolates (included pus, urine, wound, and blood) were collected from two diagnostic centers in Chennai from May 2015 to February 2016. Biofilm formation was assessed using microtiter dish biofilm formation assay and minimal inhibitory concentration (MIC) and minimal bactericidal concentrations (MBC) were determined for planktonic and biofilm cells (MBC assay). Further, PCR amplification of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 were performed. Biofilm formation was found to be moderate/strong in 16 strains. MBC for planktonic cells showed that 4, 7, 10 and 14 strains were susceptible to gentamicin, ciprofloxacin, meropenem and colistin respectively. In MBC assay for biofilm cells (MBC-B), all the 16 biofilm producing strains were resistant to ciprofloxacin and gentamicin whereas nine and four were resistant to meropenem, and colistin respectively. The biofilm-specific antibiotic-resistant genes PA0756-0757 was found in 10 strains, 6 strains with PA5033 and 9 strains with PA2070 that were found to be resistant phenotypically. This study highlighted the importance of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033, and PA2070 in biofilm-forming P. aeruginosa.

scholarly journals The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

2020 ◽  
Vol 21 (20) ◽  
pp. 7748
Author(s):  
Adrian Augustyniak ◽  
Krzysztof Cendrowski ◽  
Bartłomiej Grygorcewicz ◽  
Joanna Jabłońska ◽  
Paweł Nawrotek ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Titanium Dioxide ◽  
Biofilm Formation ◽  
Efflux Pump ◽  
Uv Light ◽  
Alternative Methods ◽  
Silica Nanotubes ◽  
Antibiotic Resistant ◽  
Metabolic Functions ◽  
Resistant Strains

Pseudomonas aeruginosa is a bacterium of high clinical and biotechnological importance thanks to its high adaptability to environmental conditions. The increasing incidence of antibiotic-resistant strains has created a need for alternative methods to increase the chance of recovery in infected patients. Various nanomaterials have the potential to be used for this purpose. Therefore, we aimed to study the physiological response of P. aeruginosa PAO1 to titanium dioxide/silica nanotubes. The results suggest that UV light-irradiated nanomaterial triggers strong agglomeration in the studied bacteria that was confirmed by microscopy, spectrophotometry, and flow cytometry. The effect was diminished when the nanomaterial was applied without initial irradiation, with UV light indicating that the creation of reactive oxygen species could play a role in this phenomenon. The nanocomposite also affected biofilm formation ability. Even though the biomass of biofilms was comparable, the viability of cells in biofilms was upregulated in 48-hour biofilms. Furthermore, from six selected genes, the mexA coding efflux pump was upregulated, which could be associated with an interaction with TiO2. The results show that titanium dioxide/silica nanotubes may alter the physiological and metabolic functions of P. aeruginosa PAO1.

scholarly journals Phenotypical Comparison of Pseudomonas Aeruginosa Isolated from Human and Veterinary Samples; Impact of Host Adaptation on Infection Pathogenesis

2021 ◽  
Author(s):  
Stefan J. Kaiser ◽  
Annalisa DeRosa ◽  
Christa Ewers ◽  
Frank Günther
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Biofilm Formation ◽  
Polymorphonuclear Leukocytes ◽  
Environmental Isolates ◽  
Antibiotic Resistant ◽  
Pet Owners ◽  
Zoonotic Risk ◽  
Genetically Determined

Abstract Purpose: Determinants of virulence in Pseudomonas aeruginosa vary strongly depending on its habitat. In this study, we analyzed these alterations depending on the host organism in isolates cultured from canine ears and compared it to clinical extended-spectrum antibiotic-resistant Pseudomonas aeruginosa isolates (XDR), clinical antibiotic-sensitive (non-XDR) from humans and environmental isolates (EI) analyzed during our first study in 2017. Methods: A total of 22 veterinary isolates cultured from canine ears (VET) were examined for spontaneous biofilm formation, stress response in biofilm formation induced by meropenem, in vitro fitness, susceptibility to human serum and polymorphonuclear leukocytes and the genetically determined virulence factors toxA, exoS, exoT, exoU, exoY, nan1, cif, lasA and lasB.Results: We observed significantly elevated spontaneous biofilm formation and serum susceptibility in VET isolates compared to EI and non-XDR isolates as well as significantly decreased in vitro fitness compared to XDR isolates. The VET isolates resembled most the XDR subgroup of isolates previously cultured from blood. Within the environmental isolates, we observed an increase of spontaneous biofilm formation and exoU presence in isolates cultured from community water samples over hospital water samples to pool samples.Conclusions: Considering the distinct differences in some features of the examined VET isolates, a higher degree of phenotypical adaption can be assumed. Increased biofilm formation seems to be a common and characteristic event in isolates adapted to a specific habitat. Therefore amplification of potentially more virulent Pseudomonas aeruginosa strains in domestic animals may lead to elevated zoonotic risk for example for pet owners.

scholarly journals Study of Biofilm Formation and Antibiotic Resistant Profile in Multidrug Resistant and Extensive Drug Resistance Pseudomonas Aeruginosa Isolated from Burn Wound Infections in Southwest Iran

2021 ◽  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Full Text ◽  
Biofilm Formation ◽  
Multidrug Resistant ◽  
Wound Infections ◽  
Burn Wound ◽  
Antibiotic Resistant ◽  
Extensive Drug Resistance ◽  
Southwest Iran

Abstract The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.

scholarly journals Two Efflux Systems Expressed Simultaneously in Multidrug-Resistant Pseudomonas aeruginosa

2000 ◽  
Vol 44 (10) ◽  
pp. 2861-2864 ◽  
Author(s):  
Lilian Pumbwe ◽  
Laura J. V. Piddock
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Promoter Region ◽  
Multidrug Resistant ◽  
Antibiotic Resistant ◽  
Upstream Promoter ◽  
Upstream Promoter Region

ABSTRACT Simultaneous overexpression of MexAB-OprM and MexEF-OprN was demonstrated for a multiply antibiotic-resistant clinical isolate ofPseudomonas aeruginosa (G49). G49 also had decreased expression of OprF. No mutations in mexR or its upstream promoter region, mexT, oprM, oprF, or sigX were revealed, suggesting regulation by a hitherto undescribed locus.

scholarly journals Association of exopolysaccharide genes in biofilm developing antibiotic-resistant Pseudomonas aeruginosa from hospital wastewater

2021 ◽  
Author(s):  
M. Divyashree ◽  
Madhu K. Mani ◽  
Indrani Karunasagar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Multidrug Resistant ◽  
Hospital Wastewater ◽  
Antibiotic Resistant ◽  
Crystal Violet Assay ◽  
Biofilm Production ◽  
Stress Environment ◽  
The Relationship

Abstract The study aimed to examine the relationship between antibiotic resistance, biofilm formation and genes responsible for biofilm formation. Sixty-six Pseudomonas aeruginosa isolates were obtained from hospital wastewater and analyzed for their antibiotic resistance. Biofilm production among the isolates was tested by quantitative method crystal violet assay. Biofilm-associated genes among these isolates psl, alg, and pel were also checked. The maximum resistance was observed for ampicillins (88.24%) followed by nalidixic (83.82%), and nitrofurantoin (64.71%), respectively. Biofilm phenotypes are distributed in the following categories: high 39.39% (n = 26); moderate 57.57% (n = 38), and weak 3.0% (n = 2). Among the total isolates, biofilm-associated genes were detected in 84.84% (n = 56) of isolates and the remaining isolates 15.15% (n = 10) did not harbor any genes. In this study, pslB was the most predominant gene observed (71.21%, n = 47) followed by pslA (57.57%, n = 38), pelA (45.45%, n = 30), algD (43.93%, n = 29), and pelD (27.27%, n = 18), respectively. The present study reveals that the majority of the isolates are multidrug resistant being moderate and high biofilm formers. The study implies that biofilm acts as a machinery for bacteria to survive in the hospital effluent which is an antibiotic stress environment.

scholarly journals Study of Biofilm Formation and Antibiotic Resistant Profile in Multidrug Resistant and Extensive Drug Resistance Pseudomonas Aeruginosa Isolated from Burn Wound Infections in Southwest Iran

2021 ◽  
Author(s):  
Aram asareh zadegan dezfuli ◽  
Arshid Yousefi Avarvand
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Biofilm Formation ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Wound Infections ◽  
Burn Wound ◽  
Antibiotic Resistant ◽  
Extensive Drug Resistance ◽  
Southwest Iran

Abstract Background: Pseudomonas aeruginosa is an opportunistic pathogen that has remained on the ‘top 10’ common hospital ‘superbugs’ worldwide for more than a decade. Study of biofilm formation and antibiotic resistant profile in multidrug resistant and extensive drug resistance P. aeruginosa isolated from burn wound infections in southwest IranMethods and Results: This study, which was performed in 110 P. aeruginosa isolates culture-positive reports. Assessment of biofilm formation via microtiteplate and congo red agar. Overall, 110 clinical P. aeruginosa isolates were confirmed from wound burn infections. The maximum resistance rate among P. aeruginosa isolates to antibiotics tested was as follow Piperacillin, ceftazidime, and minimum resistance rate among P. aeruginosa isolates to antibiotics tested such as ticarcillin-clavulanic acid. The isolates were then evaluating the MICs by using the E-test. only 7 isolates were confirmed as colistin-resistant. Colistin reference MICs for the The prevalence of MDR P. aeruginosa was 38% and XDR- P. aeruginosa was 22% respectively. One of P. aeruginosa isolates were PDR. In microtiteplate assay,76% of the isolates have ability for biofilm, formation, 40% were categorized as strong biofilm-formers; 32% were moderate; 21% were weak biofilm formers and 43% could not form any detectable biofilm. Conclusion: in our study development of resistance by P. aeruginosa to many antimicrobial agents is a great challenge in controlling its infections. Therefore, the transmission of these isolates to patients leads to higher resistance. Therefore, the necessary hygiene measurements should be taken for the prevention of transferring the P. aeruginosa isolates to hospitalized patients.

scholarly journals Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function

2001 ◽  
Vol 183 (18) ◽  
pp. 5395-5401 ◽  
Author(s):  
Morten Hentzer ◽  
Gail M. Teitzel ◽  
Grant J. Balzer ◽  
Arne Heydorn ◽  
Søren Molin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Structure And Function ◽  
Abiotic Surface ◽  
Antibiotic Resistant ◽  
Biofilm Development ◽  
And Function ◽  
Biofilm Structure ◽  
Antimicrobial Treatments ◽  
The Relationship

ABSTRACT During the course of chronic cystic fibrosis (CF) infections,Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the exopolysaccharide alginate. Chronic P. aeruginosainfections involve surface-attached, highly antibiotic-resistant communities of microorganisms organized in biofilms. Although biofilm formation and the conversion to mucoidy are both important aspects of CF pathogenesis, the relationship between them is at the present unclear. In this study, we report that the overproduction of alginate affects biofilm development on an abiotic surface. Biofilms formed by an alginate-overproducing strain exhibit a highly structured architecture and are significantly more resistant to the antibiotic tobramycin than a biofilm formed by an isogenic nonmucoid strain. These results suggest that an important consequence of the conversion to mucoidy is an altered biofilm architecture that shows increasing resistance to antimicrobial treatments.

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