scholarly journals Biocontrol Potential of <i>Bacillus amyloliquefaciens</i> Bc2 and <i>Trichoderma harzianum</i> TR against Strawberry Anthracnose under Laboratory and Field Conditions

2020 ◽  
Vol 11 (03) ◽  
pp. 260-277
Author(s):  
Rababe Es-Soufi ◽  
Houda Tahiri ◽  
Latifa Azaroual ◽  
Aïcha El Oualkadi ◽  
Patrick Martin ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Bacillus Amyloliquefaciens ◽  
Field Conditions ◽  
Strawberry Anthracnose ◽  
Biocontrol Potential

scholarly journals Using maize wastes, fermented by co-cultures of Trichoderma harzianum and Pseudomonas fluorescens, as grain dressing against m maize diseases under field conditions

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Sherien M. M. Atalla ◽  
Mokhtar M. Abdel-Kader ◽  
Nadia G. El-Gamal ◽  
Nehal S. El-Mougy
Keyword(s):  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Raw Materials ◽  
Chitosan Nanoparticles ◽  
Field Conditions ◽  
Optimum Ratio ◽  
Transmission Electron ◽  
Environmentally Safe ◽  
The Mean ◽  
Uv Visible

Abstract Maize (Zea mays L.) is one of the most economic crops in Egypt. Production of amylase from the waste of maize is the most economic and cheap renewable and most abundant raw materials present in environment. Biosynthesis of Cu-chitosan nanoparticles for amylase production by co-culturing between Trichoderma harzianum and Pseudomonas fluorescens at different ratios compared to free conditions was the main purpose of this study. The optimum ratio 8:2, recorded between P. fluorescens: T. harzianum, was the most promising for production of amylase produce 22.47 and 28.60 U/ml for free and nano, respectively. The UV visible spectral analysis Cu-chitosan NPs was 220 nm, while the mean diameter, using transmission electron microscopy was 0.5 μm. Application of fermented maize wastes by co-cultivation of P. fluorescence and T. harzianum, as a grain dressing before sowing declared the reduction in both root and foliar diseases during the maize growing season, starting from germination up to 70 days of its vegetative growth under field conditions. A promising approach is the creation and use of environmentally safe products, whose protective effect is based on the induction of hydrolase inhibitors in plants.

scholarly journals Bioeffektor talajoltóanyagok alkalmazási módszerfejlesztése tenyészedény-kísérletben paradicsommal

Economica ◽  
2020 ◽  
Vol 7 (3) ◽  
pp. 183-189
Author(s):  
Tamás Gáspár ◽  
Anita Dudás ◽  
Zsolt Kotroczó ◽  
Heléna Wass-Matics ◽  
Bence Trugly ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Bacillus Amyloliquefaciens ◽  
Pseudomonas Sp

Az elmúlt évtizedekben a műtrágyák, növényvédő szerek, talajfertőtlenítők és más vegyipari agrokémiai termékek látványosan növelték a mezőgazdaság termelékenységét. Napjainkra a sokszor gondatlan és túlzott használat – pozitív hatásaik mellett – a talajok szerkezeti romlását, savanyodását, a talajélet nagyfokú csökkenését is magával hozta. A fenntartható szemléletű termesztéshez olyan mikrobiális oltóanyagok, bioeffektor termékek nagyobb fokú felhasználására van szükség, amelyek képesek javítani a növény mennyiségi és minőségi tulajdonságait. Munkánk során Európában már bevezetett kereskedelmi oltóanyagok alkalmazásának hatásait vizsgáltuk tenyészedényes kísérletben, három bioeffektor (BE) termékkel: BE1 - Trichoderma harzianum T 22; BE2 - Pseudomonas sp.; BE3 - Bacillus amyloliquefaciens Rhizovital 42 F1. Tenyészedényes kísérletben kerestük a termékek közti különbségeket, az alkalmazás módjától és idejétől, illetve a foszfor-formák felvehetőségétől függően is. A bioeffektorokat a gyártók által javasolt módon a vetéssel egy időben adagoltuk. Megállapítottuk, hogy azok a gyomok gyorsabb kelése miatt a gyomok biomasszáját növelték, megelőzvén a paradicsomot. A leghatékonyabbnak, a P-felvételt javítani képes BE 3 oltóanyag bizonyult. A bioeffektorok alkalmazásánál ezért a növény és az alkalmazott törzsek élettani igényei, potenciálja mellett a technológiai alkalmazási lehetőségeket is javasolt figyelembe venni.

GROWTH AND OIL YIELD PARAMETERS OF THE Capsicum annuum VAR aviculare ASSOCIATED TO THE BENEFICIAL BACTERIUM Bacillus amyloliquefaciens AND Azospirillum halopraeferens UNDER FIELD CONDITIONS

Biotecnia ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 59-64
Author(s):  
José Luis García Hernández ◽  
María Cristina Ruíz Alvarado ◽  
Pablo Preciado Rangel ◽  
Alejandra Nieto-Garibay ◽  
Bernardo Murillo-Amador ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Growth And Development ◽  
Bacillus Amyloliquefaciens ◽  
Irrigation System ◽  
Total Content ◽  
Field Conditions ◽  
Agricultural Producers ◽  
Beneficial Bacterium ◽  
Bacterium Bacillus ◽  
Native Strains

Capsicum annuum var. aviculare is one of the most extremely hot chilies, measuring between 50,000 and 100,000 Scoville Units. In the present work we studied the growth and development of chiltepin (Mazocahui), under field conditions, inoculated with previously selected and cultivated native strains of Bacillus amyloliquefaciens and Azospirillum halopraeferens. The seed was inoculated by the vacuum technique, and later at 34, 120, 180 and 210 days after germination. A drip irrigation system with a salinity (TDS) = 0.8 ppt was used. The results show that germination is significantly favored by the promoter effect caused by the beneficial bacteria. These inoculants increased some parameters of growth and development. We found significant differences regarding the control for the total weight and length of root and stem of the plants, as well as the total content of proteins, and in some parts of the plant analyzed as stem and leaf. Both bacteria increased fruit yield in the Mazocahui genotype. Our findings suggest that the application of strains of Bacillus amyloliquefaciens and Azospirillum halopraeferens, influence the increase in yield of Capsicum annuum var. aviculare, under field conditions. In addition, the data show the usefulness of the inoculation of chiltepin seed for agricultural producers in semi-arid areas where salinity is a problem.

Phaseolus acutifolius ASSOCIATED WITH Bacillus amyloliquefaciens AND Azospirillum halopraeferens UNDER SALINITY CONDITIONS

Biotecnia ◽  
2019 ◽  
Vol 21 (1) ◽  
pp. 127-132
Author(s):  
R. Prabhaharan ◽  
J. Borboa-Flores ◽  
E. C. Rosas-Burgos ◽  
J. L. Cárdenas-López ◽  
J. Ortega-García ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Field Conditions ◽  
Growth And Yield ◽  
Yield Enhancement ◽  
Arid Zones ◽  
Potential Yield ◽  
Phaseolus Acutifolius ◽  
Plant Parts ◽  
Mexican State ◽  
Plant Growth Promoting

Salinity-tolerant plants offer hope for the future of agriculture by providing solutions to the problems caused by years. Sonora is the most arid Mexican state. The agroindustrial halophytes are an option in dry-arid zones agriculture. In the present study, we evaluated the growth and development under different salinity and field conditions, of two beans (Phaseolus acutifolius) genotypes: Indio Yumi, and Navojoa. Seeds were inoculated with plant growth promoting halobacteria, a previously selected and cultivated strain of Azospirillum halopraeferens and a native Bacillus amyloliquefaciens. Significant differences were observed among them for weight and biomass, as well as biochemical features between the analyzed plant parts. Our findings suggest that a potential yield enhancement and protein production under field conditions can be promoted by the application of the beneficial bacterium B. amyloliquefaciens and A. halopraeferens. Also, demonstrated the ability of the studied beneficial halobacteria to promote growth and yield of the halotolerant Phaseolus acutifolius, a potentially useful finding for the agricultural growers in dry and semiarid zones.

scholarly journals Biocontrol potential of Trichoderma harzianum in controlling wilt disease of pistachio caused by Verticillium dahliae

2017 ◽  
Vol 57 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Zeinab Fotoohiyan ◽  
Saeed Rezaee ◽  
Gholam Hosein Shahidi Bonjar ◽  
Amir Hossein Mohammadi ◽  
Mohammad Moradi
Keyword(s):  
Verticillium Dahliae ◽  
Trichoderma Harzianum ◽  
Pathogenic Fungus ◽  
Antagonistic Activity ◽  
Wilt Disease ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Biocontrol Potential

Abstract Verticillium wilt caused by Verticillium dahliae, is one of the most devastating diseases in pistachio orchards in the world including Iran. In search for an effective non-chemical strategy for the management of this disease, we evaluated the biocontrol potential of Trichoderma harzianum isolates obtained from the rhizosphere of healthy pistachio trees in different locations of the Kerman province of Iran against V. dahliae under laboratory and greenhouse conditions. Dual culture tests in the laboratory were conducted in a completely randomized design using 72 T. harzianum isolates. Twenty isolates showed the highest in vitro antagonistic activity. The results indicated that all 20 isolates were capable of inhibiting the mycelial growth of V. dahliae significantly. Among them, isolates Tr8 and Tr19 were the most effective by 88.89% and 85.12% inhibition, respectively. Extracted cell free metabolites of all effective isolates also inhibited the growth of V. dahliae in the culture medium significantly. According to the results, isolates Tr4 and Tr6 inhibited fungal pathogen growth by 94.94% and 88.15% respectively, through production of non-volatile metabolites. In the evaluation of volatile metabolites, isolates Tr5 and Tr4 were the most effective by 26.27% and 24.49% growth inhibition, respectively. Based on the results of the in vitro experiments, the five most effective isolates were selected for evaluation under greenhouse conditions for their biocontrol potential in controlling Verticillium wilt of pistachio. Results of the greenhouse, (in vivo) experiments were positive and indicated that the occurrence of wilt disease in plants treated with the antagonists alone or in combination with pathogenic fungus was lower than in plants inoculated with pathogen alone. The overall results of this study suggest that Trichoderma fungal antagonist may be an effective biocontrol agent for the control of Verticillium wilt of pistachio.

scholarly journals Mycoparasitic antagonism of a Trichoderma harzianum strain isolated from banana plants in Oaxaca, Mexico

Biotecnia ◽  
2021 ◽  
Vol 23 (1) ◽  
pp. 127-134
Author(s):  
Ana Claudia Sánchez-Espinosa ◽  
José Luis Villarruel-Ordaz ◽  
Luis David Maldonado Bonilla
Keyword(s):  
Biological Control ◽  
Developing Countries ◽  
Trichoderma Harzianum ◽  
Phytopathogenic Fungi ◽  
The Novel ◽  
Panama Disease ◽  
Banana Production ◽  
Biocontrol Potential ◽  
Identification And Characterization

Bananas are important crops in developing countries with tropical climate. In Mexico, the banana production has increased, and it must be guaranteed. The Panama disease, caused by the fungus Fusarium oxysporum f.sp. cubense threatens the current banana production, for what is necessary to implement methods to protect this crop. Fungi from genus Trichoderma are natural residents of the rhizosphere. This genus comprises mycoparasite species used to control diseases caused by phytopathogenic fungi, and also benefit plant development. In this report, we present data of the identification and characterization of the novel strain Trichoderma harzianum M110 that displays antagonism and biocontrol potential in laboratory conditions. Exploration of the rhizosphere and the endophytic microbial communities might help to identify microbes adapted to banana plants that can be incorporated in organic biological control formulations that ensure production of Fusarium-free plants and healthy fruits with export quality.

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