scholarly journals Assessment of Stress on Serum Estradiol and Cortisol Levels in Female Subordinate Naked Mole Rats Following Isolation from Natal Colony

2020 ◽  
Vol 08 (03) ◽  
pp. 9-17 ◽  
Author(s):  
Amos Onyansi Makori ◽  
Albert Wafula Nyongesa ◽  
Hesbon Odongo ◽  
Rael Jepkogei Masai
Keyword(s):  
Serum Estradiol ◽  
Cortisol Levels ◽  
Female Subordinate ◽  
Assessment Of Stress

scholarly journals Assessment Of Stress On Serum Estradiol Levels In Female Subordinate Naked Mole Rats Following Isolation From Natal Colony

2019 ◽  
Author(s):  
AMOS ONYANSI MAKORI ◽  
A. W. NYONGESA ◽  
H. ODONGO ◽  
R. J. MASAI
Keyword(s):  
Social Stress ◽  
Experimental Period ◽  
Reproductive Suppression ◽  
Significance Level ◽  
Elisa Technique ◽  
Female Subordinate ◽  
Set Up ◽  
And Control ◽  
Assessment Of Stress ◽  
Hormonal Analysis

Abstract Objective: This study investigated the effects of isolation of subordinate naked mole rats from natal colonies on their reproductive success. The study aimed at establishing whether the reproductive suppression in subordinate naked mole rats is the outcome of social stress exerted by breeding female in the colony or other unknown environmental factors within the colony set-up. Blood samples from experimental and control groups were collected on 2nd, 4th, 6th , 8th, 12th and 20th weeks of experimental period for hormonal analysis using ELISA technique. The Statistical analysis done using student t-test at 5% significance level. Results: Hormone analysis showed significant change in both cortisol (t = 8.74, P=0.01) and estradiol (t=7.15, P=0.02) of subordinate isolated females. Results showed no correlation between stress and cyclicity. Conclusively, the observed reproductive suppression among subordinate naked mole rats in natal colonies is probably due to presence of queen or other reproductive aspects that may not be directly related to stress.

Stress responses of wapiti (Cervus elaphus canadensis) to removal of velvet antler

2002 ◽  
Vol 82 (1) ◽  
pp. 11-17 ◽  
Author(s):  
N. J. Cook ◽  
A. L. Schaefer
Keyword(s):  
Stress Response ◽  
Heat Loss ◽  
Cervus Elaphus ◽  
Stress Responses ◽  
Salivary Cortisol ◽  
Plasma Cortisol ◽  
Respiration Rates ◽  
Velvet Antler ◽  
Cortisol Levels ◽  
Assessment Of Stress

An assessment of stress responses of 27 mature wapiti (Cervus elaphus canadensis) stags was conducted to test the efficacy of two methods of analgesia to reduce stress responses associated with the pain of cutting velvet antler. Analgesic methods were a Lidocaine nerve block (LIDO) and pedicle placement electrical analgesia (EA). A control group of animals (CON) was included that did not receive analgesia. Methods of assessing stress responses included heart and respiration rates, differential white blood cell counts and plasma cortisol. Assessment of stress responses also utilized infrared thermographic imaging and measurement of salivary cortisol concentrations. The latter measures were novel approaches to stress assessment in wapiti, and, as such, the study was a trial of their applicability. Measures were conducted over 2 d. Antler was harvested on day 1 and the animals were brought back to the handling facility 24 h later (day 2) for repeated measures. Heart and respiration rates were increased in response to cutting antler (P < 0.05) and declined following antler removal (P < 0.003). Reductions in eosinophils occurred over a 24-h period in all treatments and were statistically significant for the EA treatment (P < 0.014). Plasma cortisol concentrations did not demonstrate statistical differences between either treatments or days. Plasma cortisol concentrations were numerically higher for the EA animals on days 1 and 2 than for either the CON or LIDO treatments. Plasma cortisol levels tended to be higher after capture and restraint on day 2 compared to levels recorded after antler removal on day 1. Salivary cortisol levels were higher on day 2 compared to day 1 (P < 0.004). Between treatments, CON animals exhibited higher salivary cortisol levels on day 2 than EA (P < 0.05) and LIDO (P < 0.002) animals. Radiated heat loss, measured by infrared thermography, was significantly elevated in response to velveting (P < 0.00001). Increased radiated heat loss was observed for all treatments and was statistically significant for EA (P < 0.006) and CON (P < 0.02), but not for LIDO (P < 0.06). The study demonstrated that the process of harvesting velvet antler from wapiti initiates a significant stress response similar to that of other animals during practices such as capture, handling and restraint. The data further suggest that stress responses to the above husbandry practices are exacerbated by the pain of cutting antler and that animals treated with Lidocaine for pain management exhibited a lesser stress response than CON or EA treated animals. Key words: Wapiti, velvet antler, analgesia, stress, salivary cortisol, infrared thermography

Behavioral Assessment of Stress Compensation in Minipigs Transgenic for the Huntington Gene Using Cortisol Levels: A Proof-of-Concept Study

2018 ◽  
Vol 7 (2) ◽  
pp. 151-161 ◽  
Author(s):  
Verena Schuldenzucker ◽  
Robin Schubert ◽  
Lisa M. Muratori ◽  
Frauke Freisfeld ◽  
Lorena Rieke ◽  
...  
Keyword(s):  
Behavioral Assessment ◽  
Proof Of Concept ◽  
Concept Study ◽  
Stress Compensation ◽  
Cortisol Levels ◽  
Assessment Of Stress

Serum uric acid, cholesterol, and cortisol levels: Interrelationships in normal men under stress.

1970 ◽  
Author(s):  
Robert T. Rubin ◽  
Richard H. Rahe ◽  
Brian R. Clark ◽  
Ransom J. Arthur
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Cortisol Levels ◽  
Normal Men

Intimate Partner Sexual Violence: Cortisol Levels in Women Victims and their Children

2014 ◽  
Author(s):  
Mariana G. Boeckel ◽  
Ledo Daruy-Filho ◽  
Manuela Martinez ◽  
Thiago Viola ◽  
Rodrigo Grassi-Oliveira
Keyword(s):  
Sexual Violence ◽  
Women Victims ◽  
Intimate Partner ◽  
Cortisol Levels

The Relationship Between Parental Stress, Children's Cortisol Levels, and Temperament

2008 ◽  
Author(s):  
Mark Staniszewski ◽  
Kunaal Pai ◽  
Darren Van Buren ◽  
Penny Clark ◽  
H. Hill Goldsmith
Keyword(s):  
Parental Stress ◽  
Cortisol Levels ◽  
The Relationship

Efek Kadar Serum Estradiol Fase Bleeding Siklus Menstruasi Pada Aktivitas Fibroblas Dermis Manusia

2019 ◽  
Vol 45 (1) ◽  
Author(s):  
Graciella Regina ◽  
Sri Awalia Febriana ◽  
Yohanes Widodo Wirohadidjojo
Keyword(s):  
Mtt Assay ◽  
Ex Vivo ◽  
Serum Estradiol ◽  
Sirius Red

Pembedahan elektif selama fase bleeding siklus menstruasi sering dihindari terkait gangguan koagulasi. Salah satu hormon yang berhubungan dengan proses penyembuhan luka adalah estrogen. Kadar estrogen berfluktuasi sepanjang siklus menstruasi dan berada pada kadar terendah selama fase bleeding. Penelitian eksperimental ex vivo dilakukan pada 16 perempuan berusia 18–40 tahun yang memiliki siklus menstruasi teratur. Darah vena subjek diambil sebanyak 5ml pada fase bleeding dan ovulasi. Kemampuan penyembuhan luka dari masing-masing serum dinilai dengan mengukur proliferasi fibroblas dan deposisi kolagen fibroblas kulit. Ovulasi ditentukan dengan uji pakis saliva, kadar estradiol serum diukur menggunakan Cobas Elecsys®, proliferasi fibroblas menggunakan MTT assay, dan deposisi kolagen dengan sirius red. Hasil penelitian menunjukkan rerata kadar serum estradiol pada fase bleeding dan ovulasi berturut-turut adalah 29,6±10,5pg/dl dan 180,1±164,5pg/dl. Rerata indeks proliferasi fibroblas yang dipajankan pada fase bleeding dan ovulasi adalah 1,09±0,63 dan 1,44±0,66. Rerata densitas optik kolagen fibroblas yang terpajan serum fase bleeding dan ovulasi adalah 0,47±0,2 dan 0,54±0,14. Seluruhnya menunjukkan perbedaan yang bermakna secara statistik (p<0,05). Serum fase bleeding memiliki kemampuan penyembuhan luka yang lebih rendah dibandingkan dengan serum fase ovulasi. Kebijakan untuk tidak melakukan pembedahan elektif selama fase bleeding, selain terkait dengan gangguan pembekuan darah juga terkait dengan proses penyembuhan luka.Kata Kunci:  menstruasi, estradiol, ovulasi, proliferasi fibroblas, deposisi kolagen, penyembuhan luka

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