A Case Report on Effective Management of Artavadushti with Vandhyatwa by Ayurveda Regimen

ABSTRACT: A female patient of 25 years of age came to OPD of Arogyam Health Care on 15\5\2076 B.S. with complaints of want issue since 3 years. She had not achieved her menses since 3 months and her menstruation was irregular since menarche. Methodology: Detailed history with all necessary clinical, physical examination and laboratory investigations were carried out. No gross physical and chemical abnormality was found. All the laboratory investigations (including USG and hormonal analysis) were found to be normal. So the treatment was planned according to the symptoms. Diagnosis made on the basis on the basis of presenting complaints was Vandhaytwa. Patient was treated with Arogyavardhi vati, Dashmoolarista, Kanchanar guggulu, Matra vasti and nasya with Mahanarayan taila, Bandhyaharan churna and Aswagandha churna. Patient was kept on follow. Result: Patient had got her menstruation regularly with normal duration while taking medicine. Also the amount of flow was also improved and the patient got conceived. Keywords: Vandhyatwa, Vasti, Nasya, Arogyavardhini vati, Dashmoolarishta

Pituitary Tumours

The chapter focuses on understanding the latest classification of the pituitary adenomas in light of immuno-histological and molecular signatures as envisaged in the latest WHO classification guidelines. It further looks into evaluating and analysing the symptoms of the adenoma locally and at distant organs. Imaging and hormonal analysis has been discussed in detail for both functional, non-functional and pituitary apoplexy. Further, the therapeutic options- medical, surgical and their outcomes have been highlighted.

Combined Effect of Monieziosis and Hypomicroelementosis on Some Hematological, Biochemical and Hormonal Parameters in Merino Sheep

This study was done to determine the impact of monieziosis infection combined with hypomicroelementosis on some hematological, biochemical and hormonal parameters of Soviet Merino sheep in the Astrakhan region. 20 sheep, aging 3 years old and average 43±1.6 kg body weight were used. Sheep were divided into two groups. First group contained 10 sheep and these sheep were naturally infected with monieziosis and clinically were suffered from hypomicroelementosis, emaciation, reduced growth rate, anemia, diarrhea and pale mucosa. Second group contained 10 sheep were apparently healthy and free from internal parasites and they were used as a control group. The first group was given Praziver (praziquantel and ivermectin) for treatment monieziosis, while they intramuscularly injected with Sedimin (selenium, iodine and iron) and were introduced daily into the feed with CoCl2. Faecal and blood samples from both groups were collected, before and 30 days after treatment, and analyzed for some hematological, biochemical and hormonal parameters. Our results revealed that there was a significant decrease in Hb and RBCs values, while total WBCs and eosinophils were significantly increased in the diseased group than in healthy one. Biochemical analysis showed a significant decrease in serum antioxidant enzymes (CAT, SOD and GSH-Px) and a significant increase in serum DC and MDA in the diseased group as compared with the control group. Hormonal analysis showed a significant increase in ACTH, TSH, Cortisol and a significant decrease in serum T4 and T3. After treatment with Praziver, Sedemin and CoCl2, there was a significant effectiveness to maintain blood parameters within normal levels in the experimental group and increase reproductive outcome from these sheep

Biochemical Assessment of Fertility-Enhancing Effect of Aqueous Extract of Symphonia globulifera (Linn. F.) In Adult Male Wistar Rats

The present study investigated the effect of oral administration of aqueous extract of Symphonia globulifera root on fertility in addition to its effect on some biochemical indices in adult male Wistar rats. After the aqueous extraction, phytochemical screening was carried out. Twenty-four (24) mature and healthy adult male wistar rats of weights between 150-220 g were used. The rats were randomly divided into four (4) groups each containing six (6) rats. Group 1 rats were gavaged with distilled water which served as control group. Group 2, 3 and 4 rats were orally treated with 100, 200 and 400 mg/kg body weight of the aqueous extract of S. globulifera respectively for 21 days. After the treatment, the rats were sacrificed and their organs (heart, liver, kidney and testes) were excised. Hormonal analysis which include seminal fluid analysis (SFA), follicle stimulating hormone (FSH), leutenizing hormone (LH), testosterone and prolactin were carried out following standard procedures. The data were analysed using students t-test and ANOVA with the aid of SPSS with P < 0.05 level of statistical significance. The phytochemical screening of the extract revealed presence of major phytochemicals. There was dose-dependent decrease in the body weight of the rats after the treatment. In hormonal analysis, SFA increased significantly when compared to that of the control rats. The analysis also revealed that the blood level of follicle stimulating hormone (FSH) was significantly increased whereas; there was decrease in testosterone level when compared with the control animals. In conclusion, aqueous extract of S. globulifera was found to be safe and showed significant fertility enhancing effects.

EFFECTIVE MANAGEMENT OF ARTAVA KASHAYA BY AYURVEDA: A CASE REPORT

A female patient of 25 years of age came to OPD of National institute of Ayurveda, Jaipur on 22 September, 2020 with chief complaint of delayed menstruation with scanty flow since 4.5 years. Methodology: Detailed history with all necessary clinical, physical examination and laboratory investigations were carried out. No gross physical and chemical abnormality was found. All the laboratory investigations (including USG and hormonal analysis) were found to be normal. So the treatment was planned according to the symptoms. Diagnosis made on the basis on the basis of presenting complaints was Artava kashaya. Patient was treated with Tilashelukaravi kwatha for 2 consecutive cycles. Patient was kept on follow up for 1 cycle after cessation of Tilashelukaravi kwatha. Result: Patient had got her menstruation regularly with normal duration while taking medicine. Also the amount of flow was also improved and intensity of pain was reduced effectively from moderate to mild. Even after cessation of medicine she got her menstruation in 30 days with normal amount of flow.

Endocrine Dysfunction in Traumatic Subarachnoid Hemorrhage: A Prospective Study

Background This study has prospectively investigated pituitary function and their correlation with severity, pressure effect, and Glasgow outcome scale in the acute phase of traumatic subarachnoid hemorrhage (SAH). Most of the retrospective studies have demonstrated that traumatic SAH-mediated hypopituitarism could be more frequent than previously known. Objectives The aim of the study is to find the prevalence of endocrine dysfunction in traumatic SAH and its correlation with severity of injury and final outcome. Materials and Methods Eighty-four consecutive patients of traumatic SAH formed the study group. Apart from clinical assessment, noncontrast computed tomography of the head was performed on all patients on admission. The hormonal analysis (FT3, FT4, thyroid-stimulating hormone, growth hormone [GH], cortisol, prolactin, testosterone) was performed within 24 hours of traumatic brain injury and was repeated on the seventh day amongst the patients who survived. Results Most common hormone to increase on day one was cortisol (48.78%), while on day seven follicle-stimulating hormones and cortisol (15.38%) showed increment in levels. Most common hormone to decrease on day one was FT3 (36.84%) and GH (36.26%), while on day seven testosterone (66.67%) and FT4 (30.76%) showed decreasing levels. Hormone most resistant to change was prolactin. Conclusion Hormonal dysfunction is common in moderate to severe traumatic brain injury. There is a direct association between radiological grading (Fischer) of SAH and hormonal profile changes. Performance of hormonal analysis should be considered in patients with moderate to severe traumatic brain injury, preferably with high-grade SAH, so that appropriate hormonal replacement can be done to optimize the clinical outcome.

Association of SNP T125A on KiSS1 gene with reproduction hormone levels in Kaligesing goat

The objective of this study was to assess the association of KiSS1 gene polymorphism with reproductive traits in Kaligesing goat. Genotypes of 48 ewes aged three years old were determined using PCR-RFLP and DNA sequencing. Sixteen out of 48 samples were monitored for the estrus cycle and determined their 17β-estradiol and progesterone levels using ELISA method. The results showed that KISS1 gene in the studied population was polymorphic with one single nucleotide polymorphism (SNP T125A). Then SNP was used to analyze genotype all individuals by PCR-RFLP method using MboII enzyme. Three genotypes (TT, TA and AA) were identified. Genotype frequencies of TA were 59.57%, AA were 40.43% while TT was not inculed in calculation for genetic diversity and assosication analysis since the number of genotype only one goat. The allele frequencies of T and A were 29.79% and 70.21%, respectively. The genotype distribution for the SNP was deviated from Hardy-Weinberg equilibrium (χ2=8.10; P<0.025). Hormonal analysis showed that the levels of 17β-estradiol in the follicular and luteal phase were 50.12±61.26 and 42.35±42.43 pg/ml, respectively, while the levels of progesterone hormone in the follicular and luteal phase were 7.87±10.59 and 12.39±15.93 ng/ml. No significant associations of the polymorphism were observed for any hormonal levels. However, it was first report about polymorfism in KiSS1 gene of local Indonesian goat especially Kaligesing goat.

Association of GRIA1 polymorphisms with ovarian response to human menopausal gonadotropin in Iranian women

Objective: Glutamate ionotropic receptor AMPA type subunit 1 (GRIA1) is a subunit of a ligand-gated ion channel that regulates the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) by controlling the release of gonadotropin-releasing hormone. Few studies have investigated the association between the GRIA1 gene and human infertility. This study evaluated the association of the GRIA1 rs548294 C > T and rs2195450 G > A polymorphisms with the ovarian response to human menopausal gonadotropin (HMG) in Iranian women.Methods: One hundred women with histories of at least 1 year of infertility were included. On the second day of menstruation, patients were injected with HMG; on the third day, blood samples were collected. After hormonal analysis, the GRIA1 rs548294 C > T and rs2195450 G > A genotypes of samples were identified via the restriction fragment length polymorphism method, and on day 9, the number of follicles was assessed via ultrasound.Results: For the GRIA1 rs548294 C > T and rs2195450 G > A single nucleotide polymorphisms, the subjects with CT and GG genotypes, respectively, displayed the highest mean FSH level, LH level, and number of follicles on day 9 of the menstrual cycle (p< 0.05). Significant positive correlations were observed between LH and FSH (p< 0.01), LH and follicle count (p< 0.01), FSH and age (p< 0.05), follicle count and age (p= 0.048), and FSH and follicle count (p< 0.01).Conclusion: This study showed a significant relationship between GRIA1 polymorphisms and ovarian response to the induction of ovulation. Therefore, determining patients’ GRIA1 genotype may be useful for improving treatment and prescribing suitable doses of ovulation-stimulating drugs.

On the occasion of the anniversary of Nikolay P. Goncharov

In January 2020, the famous scientist biochemist-endocrinologist, MD, professor Nikolay P. Goncharov turned 85 years old. Nikolay P. Goncharov one of the leading experts in the field of biochemical endocrinology and hormonal analysis; he developed and introduced in the framework of the Human Reproduction program an experimental model for evaluating the pharmacokinetics, mechanism of action, efficacy and safety of new steroid drugs and regulators of endocrine secretion of steroid hormones. The editorial board of the journal Problems of Endocrinology congratulates NP Goncharov on his anniversary, wishes him good health, success, prosperity, vitality, fortitude and many more years of fruitful activity.