scholarly journals Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds

2013 ◽  
Vol 12 (3) ◽  
pp. 3630-3642 ◽  
Author(s):  
T. Andrade ◽  
E.V.R. Von Pinho ◽  
R.G. Von Pinho ◽  
G.E. Oliveira ◽  
V. Andrade ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stages Of Development ◽  
Heat Resistant ◽  
Maize Seeds ◽  
Physiological Quality

scholarly journals Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying

2015 ◽  
Vol 37 (2) ◽  
pp. 102-110
Author(s):  
Leandra Helena do Nascimento Gonçalves ◽  
Heloísa Oliveira dos Santos ◽  
Édila Vilela de Resende Von Pinho ◽  
Thaís de Andrade ◽  
Iolanda Vilela Von Pinho ◽  
...  
Keyword(s):  
Gene Expression ◽  
Water Content ◽  
Seed Quality ◽  
Isocitrate Lyase ◽  
Electrophoretic Pattern ◽  
Drying Methods ◽  
Heat Resistant ◽  
Expression Of Genes ◽  
Physiological Quality

Handroanthus serratifolius seeds are considered orthodox and have some mechanisms that assist in desiccation tolerance, like heat-resistant proteins and the presence of protective systems against free radical production. In order to investigate the drying effect on the quality of Handroanthus serratifolius seeds collected with 28% water content two methods were used: slow drying (30 °C) and fast drying (45 °C) until water contents equaled 20%, 15%, 10% and 5%. Seed quality was assessed by germination test, first count of germination, speed germination, emergence and speed emergence. The expression of heat-resistant proteins and enzymes catalase, esterase and isocitrate lyase was assessed by electrophoresis. The gene expression of sHSP17.5, CAT3 and ICL6 was quantified by real time PCR. Higher values of germination and vigor were obtained in seeds with 20% water content, subjected to fast drying. There was induction of heat-resistant proteins expression for both drying methods. There was variation in the electrophoretic pattern of catalase, esterase and isocitrate lyase in seeds subjected to different treatments. The gene expression in Handroanthus serratifolius seeds varies depending on methods of drying and water content of seeds. The drying of Handroanthus serratifolius seeds until the water content between 15 and 10% compromises their physiological quality.

scholarly journals Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

2015 ◽  
Vol 14 (3) ◽  
pp. 8623-8633 ◽  
Author(s):  
G.E. Oliveira ◽  
E.V.R. Von Pinho ◽  
T. Andrade ◽  
J.C. Souza ◽  
F. Caixeta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amylase Gene ◽  
Maize Seeds ◽  
Physiological Quality

scholarly journals Heat-induced expression of albumin during early stages of rat embryo development.

1987 ◽  
Vol 7 (12) ◽  
pp. 4599-4602 ◽  
Author(s):  
U K Srinivas ◽  
C J Revathi ◽  
M R Das
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Embryonic Liver ◽  
Rat Embryo ◽  
Adult Liver ◽  
Stages Of Development ◽  
Induced Expression ◽  
Molecular Events ◽  
Internal Change ◽  
Shock Proteins

An examination of heat-induced expression of proteins in tissues from adult and embryonic liver in rats shows that albumin, which is constitutively expressed in adult liver and is not synthesized in embryos before 16 days of gestation, appears in liver cells at earlier stages of development upon heat shock. On the basis of available evidence for the expression of heat shock proteins at distinct stages of development and on the basis of our findings, it may be argued that there could be common molecular events taking place during development and as a result of heat shock. We suggest also that one of the consequences of heat shock could be an internal change of pH within the cell which, in turn, might trigger alterations in gene expression.

scholarly journals The thyroid hormone receptor gene (c-erbA alpha) is expressed in advance of thyroid gland maturation during the early embryonic development of Xenopus laevis.

1991 ◽  
Vol 11 (10) ◽  
pp. 5079-5089 ◽  
Author(s):  
D E Banker ◽  
J Bigler ◽  
R N Eisenman
Keyword(s):  
Gene Expression ◽  
Thyroid Hormone ◽  
Thyroid Gland ◽  
Xenopus Laevis ◽  
Hormone Receptor ◽  
Hormone Receptors ◽  
Thyroid Hormone Receptor ◽  
Thyroid Hormone Receptors ◽  
Stages Of Development ◽  
Xenopus Development

The c-erbA proto-oncogene encodes the thyroid hormone receptor, a ligand-dependent transcription factor which plays an important role in vertebrate growth and development. To define the role of the thyroid hormone receptor in developmental processes, we have begun studying c-erbA gene expression during the ontogeny of Xenopus laevis, an organism in which thyroid hormone has well-documented effects on morphogenesis. Using polymerase chain reactions (PCR) as a sensitive assay of specific gene expression, we found that polyadenylated erbA alpha RNA is present in Xenopus cells at early developmental stages, including the fertilized egg, blastula, gastrula, and neurula. By performing erbA alpha-specific PCR on reverse-transcribed RNAs from high-density sucrose gradient fractions prepared from early-stage embryos, we have demonstrated that these erbA transcripts are recruited to polysomes. Therefore, erbA is expressed in Xenopus development prior to the appearance of the thyroid gland anlage in tailbud-stage embryos. This implies that erbA alpha/thyroid hormone receptors may play ligand-independent roles during the early development of X. laevis. Quantitative PCR revealed a greater than 25-fold range in the steady-state levels of polyadenylated erbA alpha RNA across early stages of development, as expressed relative to equimolar amounts of total embryonic RNA. Substantial increases in the levels of erbA alpha RNA were noted at stages well after the onset of zygotic transcription at the mid-blastula transition, with accumulation of erbA alpha transcripts reaching a relative maximum in advance of metamorphosis. We also show that erbA alpha RNAs are expressed unequally across Xenopus neural tube embryos. This differential expression continues through later stages of development, including metamorphosis. This finding suggests that erbA alpha/thyroid hormone receptors may play roles in tissue-specific processes across all of Xenopus development.

scholarly journals Gene expression in the phenotypically plastic Arctic charr (Salvelinus alpinus): A focus on growth and ossification at early stages of development

2018 ◽  
Vol 21 (1) ◽  
pp. 16-30 ◽  
Author(s):  
Samantha V. Beck ◽  
Katja Räsänen ◽  
Ehsan P. Ahi ◽  
Bjarni K. Kristjánsson ◽  
Skúli Skúlason ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salvelinus Alpinus ◽  
Arctic Charr ◽  
Stages Of Development ◽  
Early Stages

scholarly journals Enzyme activities and gene expression in dry maize seeds and seeds submitted to low germination temperature

2016 ◽  
Vol 11 (33) ◽  
pp. 3097-3103 ◽  
Author(s):  
Costa Silva Neta Izabel ◽  
Vilela de Resende Von Pinho Edila ◽  
Maria de Abreu Viviane ◽  
Oliveira dos Santos Heloisa ◽  
Rezende Vilela Danielle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activities ◽  
Maize Seeds ◽  
Germination Temperature

scholarly journals Importance of amylases for physiological quality in maize seeds

Biotemas ◽  
2017 ◽  
Vol 30 (3) ◽  
pp. 1 ◽  
Author(s):  
Camila Aparecida Lopes ◽  
Maria Laene Moreira de Carvalho ◽  
Heloisa Oliveira dos Santos ◽  
Dayliane Bernardes de Andrade
Keyword(s):  
Maize Seeds ◽  
Physiological Quality

A qualidade de sementes está associada à soma dos atributos genéticos, físicos, fisiológicos e sanitários que afetam sua capacidade para realizar as funções vitais, estando relacionada à germinação, ao vigor e à longevidade. A expressão de genes associados à qualidade fisiológica pode ser avaliada por meio de análises de germinação e vigor, e ainda pelas análises de transcritos e de proteínas em sementes. Objetivou-se com este trabalho fazer uma revisão sobre a relevância das enzimas do grupo das amilases relacionadas à qualidade fisiológica de sementes de milho. Dentro do grupo das amilases, a α-amilase (1,4-α-D-glucan-glucanohydrolase E.C 3.2.1.1) é uma enzima importante na hidrólise do amido, sendo responsável por 90% da atividade amilolítica em sementes de milho. Essa enzima, a exemplo da dextrose, causa a conversão de amido em açúcares que são utilizados no crescimento do embrião. Já a β-amilase (1,4-α-glucan maltohydrolase E.C 3.2.1.2) é uma α-1,4-D-glucano maltohidrolise que catalisa a liberação de maltose e dextrinas a partir das extremidades não redutoras do amido. Pesquisas comprovam que as enzimas amilases estão ligadas diretamente à qualidade fisiológica de sementes de milho. A α-amilase e a β-amilase estão envolvidas principalmente no processo de germinação e na heterose das sementes, podendo ser também utilizadas como marcadores moleculares relacionadas à tolerância de secagem das sementes. 

scholarly journals Physiological quality of hybrid maize seeds through respiratory and enzymatic activities

2016 ◽  
Vol 11 (20) ◽  
pp. 1879-1886 ◽  
Author(s):  
Oliveira dos Santos Heloisa ◽  
Vilela Von Pinho Iolanda ◽  
Vilela de Resende Von Pinho Eacute dila ◽  
Maria de Oliveira Pires Raquel ◽  
Fabiana da Silva Valk iacute ria ◽  
...  
Keyword(s):  
Enzymatic Activities ◽  
Maize Seeds ◽  
Physiological Quality ◽  
Hybrid Maize

Heat-resistant protein expression during germination of maize seeds under water stress

2016 ◽  
Vol 15 (3) ◽  
Author(s):  
V.M. Abreu ◽  
I.C. Silva Neta ◽  
E.V.R. Von Pinho ◽  
G.M.F. Naves ◽  
R.M. Guimarães ◽  
...  
Keyword(s):  
Water Stress ◽  
Protein Expression ◽  
Heat Resistant ◽  
Maize Seeds
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