scholarly journals Physiological quality of hybrid maize seeds through respiratory and enzymatic activities

2016 ◽  
Vol 11 (20) ◽  
pp. 1879-1886 ◽  
Author(s):  
Oliveira dos Santos Heloisa ◽  
Vilela Von Pinho Iolanda ◽  
Vilela de Resende Von Pinho Eacute dila ◽  
Maria de Oliveira Pires Raquel ◽  
Fabiana da Silva Valk iacute ria ◽  
...  
2017 ◽  
Vol 38 (3) ◽  
pp. 1179 ◽  
Author(s):  
Camila Segalla Prazeres ◽  
Cileide Maria Medeiros Coelho

The hydration pattern associated with the physiological quality of seeds can be an early indicator for the evaluation of seed quality. The present study aimed to evaluate the hydration pattern of seeds subjected, or not, to water restriction, and to relate these patterns to their physiological quality. The physiological quality of seeds from five maize strains (L1, L2, L3, L4, and L5) and four hybrids (HT1, HT2, HS1, and HS2) was initially determined using seed germination and vigor tests (accelerated ageing and electric conductivity). A completely randomized experimental design was used with four repeats for the physiological analyses and a regression analysis was performed in order to assess the behavior of the seeds over time. The hydration curve was determined by imbibing the seeds in distilled water (0.0 control) and in a -0.9-MPa osmotic solution of polyethylene glycol 6000. Water uptake by the seeds was found to follow a triphasic pattern in the absence of water stress (0.0 MPa); however, when exposed to a -0.9- MPa osmotic potential, an extended phase II was observed. Diversity was observed between the strains in terms of the hydration pattern of the seeds, which can influence the physiological quality of the hybrid seeds. Regarding the hybrids, a positive relationship was observed between the shortest time to root protrusion and the quality of the seeds, both with or without water restriction, which allows us to conclude that hydration pattern can be used as a parameter for the early evaluation of physiological quality in hybrid maize seeds.


Author(s):  
Helis Maria Salomão ◽  
Silvia Rahe Pereira ◽  
Andreia Suchoronczek ◽  
Adriano Suchoronczek ◽  
Larissa Leite Momoli ◽  
...  

AbstractThe aim of this study was to evaluate the physiological quality of hybrid maize seeds (within the validity period and expired seeds) and of a Creole variety within the validity period. The study was performed using the hybrids C1 BM3063 PRO 2 (valid until 12/2018) and C1 SYN8A98 (valid until 03/2017 - expired one year ago) and a Creole maize (freshly harvested, collected at Pato Branco city, Paraná State, Brazil). Seed germination, germination speed index, germination speed, cold test, length and fresh and dried matter of shoot and root system were evaluated in a laboratory experiment. In the field we evaluated emergency, emergency speed index, emergency speed, length and fresh and dried matter of aerial part. Creole cultivar had the worse performance in all the variables tested. Expired hybrid (SYN8A98) presented similar development to the hybrid within the validity period (BM3063) in practically all the evaluated parameters. SYN 8A98 hybrid can be sown without risks of reduced productivity, provided that cold periods during field establishment are avoided. Creole variety in question did not perform satisfactorily and could not be used as seed. Keywords: Seeds Validity Period. Open Pollinated Variety. Viability. Production Costs. Seeds Reuse. ResumoO objetivo deste estudo foi avaliar a qualidade fisiológica de sementes de milho híbrido (dentro do período de validade e sementes expiradas) e de uma variedade crioula dentro do período de validade. O estudo foi realizado utilizando os híbridos C1 BM3063 PRO 2 (válido até 12/2018) e C1 SYN8A98 (válido até 03/2017 - expirou há um ano) e um milho crioulo (recém-colhido, coletado na cidade de Pato Branco, Paraná, Brasil). Foram avaliados, em experimento laboratorial, a germinação de sementes, o índice de velocidade de germinação, a velocidade de germinação, o teste de frio, o comprimento e matéria fresca e seca da parte aérea e sistema radicular. No campo foram avaliados emergência, índice de velocidade de emergência, velocidade de emergência, comprimento e matéria fresca e seca da parte aérea. A cultivar crioula teve o pior desempenho em todas as variáveis testadas. O híbrido expirado (SYN 8A98) apresentou desenvolvimento similar ao híbrido dentro do período de validade (BM3063) em praticamente todos os parâmetros avaliados. O híbrido SYN 8A98 pode ser semeado sem riscos de produtividade reduzida, desde que sejam evitados períodos frios durante o estabelecimento em campo. A variedade crioula em questão não teve desempenho satisfatório e não pôde ser usada como semente. Palavras-chave: Período de Validade das Sementes. Variedade de Polinização Aberta. Viabilidade. Custos de Produção. Reutilização de Sementes. 


Author(s):  
Fernando H. B. Machado ◽  
Andréia M. S. de S. David ◽  
Silvânio R. dos Santos ◽  
Josiane C. Figueiredo ◽  
Cleisson D. da Silva ◽  
...  

ABSTRACT Crop strategies focused on the rational use of water are required in semiarid regions. Thus, the objective of this work was to evaluate the physiological quality of maize seeds produced under soil water deficit conditions. Five irrigation water depth were established, based on the field capacity (100, 85, 70, 55 and 40%), to control the available water for two maize hybrids (2B-587 and DKB-390). A randomized block design was used, with a split-plot arrangement and four repetitions. The plots consisted of irrigation water depths, and the subplots consisted of maize hybrids. The seed water concentration, germination, and vigor were determined after the harvest, using data from first germination counting, seedling emergence, germination speed index, seedling length, and accelerated aging, electrical conductivity, and modified cold tests. The maize hybrid DKB-390 showed better physiological potential under the soil water deficit conditions evaluated. The irrigation water depths lower than 70% of field capacity resulted in decreases in soil water contents and affected negatively the physiological quality of the maize seeds produced.


2021 ◽  
Vol 43 ◽  
Author(s):  
Júlia Camargos da Costa ◽  
Everson Reis Carvalho ◽  
Izabel Costa Silva Neta ◽  
Milena Christy Santos ◽  
Luciano Dias Cabral Neto ◽  
...  

Abstract: This study aimed to evaluate the effect of genetic composition and arrangement between female and male parents on tolerance to delayed drying of maize seeds, evaluating the physiological quality and enzyme expression. Ears were harvested close to the stage of physiological maturity (around 35% moisture) and the genotypes were identified as line 1 (L1), line 2 (L2), the hybrid (HB - ♀L1 and ♂L2), and the reciprocal hybrid (HR - ♀L2 and ♂L1). For assessment of physiological quality, CDR (4x6x2) was used, consisting of four genotypes, six times of delay before artificial drying (10, 18, 24, 28, 32, and 40 hours), and two drying delay temperatures (42 and 48 °C). DIC (4x3) was used for enzymatic expression, consisting of four genotypes and three delay times before artificial drying (10, 24 and 40 hours) at 48 °C. Analysis of variance F (p < 0.05), Tukey’s test (p < 0.05), and analysis of polynomial regressions were performed on the data. Lineage arrangement affects seed tolerance to drying delay. Therefore, susceptible lines should not be used as female parents. The seeds of the line most susceptible (L2) to delay in drying exhibit less expression of α-amylase (α-AM).


2016 ◽  
Vol 10 (6) ◽  
pp. 819-823
Author(s):  
Thiago Alberto Ortiz ◽  
◽  
Marcio Antônio Nicoletti ◽  
Lúcia Sadayo Assari Takahashi ◽  
◽  
...  

2018 ◽  
Vol 40 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Daniele Nerling ◽  
Cileide Maria Medeiros Coelho ◽  
Adriele Brümmer

Abstract: Viability and vigor of seeds are physiological attributes influenced by a myriad of factors including the genotype. Biochemical profiling of maize seeds and its correlation with their physiological quality is of crucial importance in breeding programs that the aim is seeds of better quality. The main goal of this study was to analyze the genetic diversity among maize inbred lines and evaluate the biochemical components that have an important role in physiological quality of seeds. The seeds of the inbred lines and hybrids were submitted to viability test, vigor by the accelerated aging and cold tolerance, and biochemical profiling (total protein content, soluble proteins, total phosphorus, phytate, inorganic phosphorus, starch and soluble sugars). The genetic divergence between the inbred lines found, prompt us to conclude that genetic gains may exist in association with physiological quality of maize hybrids. Seeds with higher inorganic phosphorus and soluble sugars presented superior physiological quality than seeds with lower levels of those compounds.


2021 ◽  
Vol 8 ◽  
pp. 1-15
Author(s):  
Itala Thaísa Padilha Dubal ◽  
Ivan Ricardo Carvalho ◽  
João Roberto Pimentel ◽  
Cristian Troyjack ◽  
Vinícius Jardel Szareski ◽  
...  

Storage is a factor that can affect seed quality as a function of latent damages. The losses that most contribute to the reduction in germination and vigor during storage are those caused by humidity, mechanical and temperature-related, that may interfere with seed quality throughout storage. The aim of this study was evaluating the physiological quality of maize seeds produced in two environments and classified in different formats compared to storage periods. The experiment was carried out in the 2016 harvest in two cultivation environments: Entre-Ijuís, RS and Pelotas, RS. Seeds were stratified by thickness through sieves with oblong holes and stratified into small round, large round, small flat and large flat. Growing environments influence the physiological characteristics of maize seeds. The flat and large format seeds have superior physiological quality for the measured physiological characters. The small and round seeds present lower values for germination, first germination count, emergence speed index, shoot dry matter of emergence seedlings and shoot dry matter of germination seedlings. Corn seeds when stored under controlled conditions remain for long periods without causing decreases in physiological quality.


2019 ◽  
Vol 11 (3) ◽  
pp. 482
Author(s):  
Bárbara Gomes Ribeiro ◽  
Raquel Maria de Oliveira Pires ◽  
Tatiana Botelho Fantazzini ◽  
Hesoisa Oliveira dos Santos ◽  
Dayliane Bernardes de Andrade ◽  
...  

The current system of seeds production is highly mechanized and these operations can contribute to losses in quality due the incidence of mechanical damages. The objective in this work was to study the effect of the chemical treatment and the storage on quality of maize seeds with mechanical damages. The seeds used were individually analyzed through X-ray test for the obtainment of three lots; 1-seeds without separation, 2-seeds without mechanical damages and 3-seeds with mechanical damages. The lots were evaluated according the physiological quality, sanity and enzymatic analyzes before and after the chemical treatment with the insecticide Maxim Advanced&reg; and the fungicide Cruiser&reg; and also after 90 days of storage. The chemical treatment of maize seeds is efficient in the control of pathogens, like Fusarium sp. The performance of seeds with internal and external damages can be maintained for 90 days when treated with fungicides and insecticides and stored in environment conditions. The isoenzymatic patterns of catalase (CAT), superoxide dismutase (SOD), malate dehydrogenase (MDH) and alcohol dehydrogenase (ADH), confirmed the higher deterioration of non-treated seeds during the storage. The mechanical damages found in seeds contribute to the reduction of viability and vigor of maize seeds.


2020 ◽  
Vol 9 (10) ◽  
pp. e7269108687
Author(s):  
Ítala Thaísa Padilha Dubal ◽  
Ivan Ricardo Carvalho ◽  
João Roberto Pimentel ◽  
Cristian Troyjack ◽  
Vinícius Jardel Szareski ◽  
...  

The aimed to evaluate the physical and physiological quality, as well the field initial performance of different maize seeds size, produced in two growing environments.  It were used seeds of the Amarelo maize (Zea mays L.) genotype, where it were produced in two growing environments at Rio Grande do Sul, in the crops season of 2016/2017. The environment that the seeds are produced and the seeds form affect the maize plants initial growth. Flat seeds are tend to have a better performance on field in relation to the initial growth. The seedlings originated from Entre Ijuís-RS present superiority to shoot length, stem diameter, roots dry matter and shoot dry matter, while Pelotas-RS determines the sieves yield. The round and small seeds present lower sieves yield, shoot length, root length and stem diameter of maize seedlings.


2014 ◽  
Vol 36 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Viviane Maria de Abreu ◽  
Édila Vilela de Resende Von Pinho ◽  
Renzo Garcia Von Pinho ◽  
Glória Maria de Freitas Naves ◽  
Izabel Costa Silva Neta ◽  
...  

Early characterization of genotypes, through the previous assessment of physiological quality of their seeds and their enzymatic systems, can provide parameters capable of aiding in the selection of new maize cultivars tolerant to water stress. Thus, this study was aimed at assessing seed performance of five different maize lines subjected to four different water stress levels. The physiological quality of seeds germinated under the osmotic potentials of 0.0, -0.3, -0.6 and -0.9 MPa was assessed by germination test, number of strong normal seedlings and T50, as well as by the expression of the isozymes superoxide dismutase (SOD), catalase (CAT), glutamate oxaloacetate transaminase (GOT), esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and of heat-resistant proteins. The osmotic potential of -0.9 MPa affected the physiological quality and vigor of seeds of all maize lines assessed. The highest values for the germination percent, in the lowest osmotic potentials assessed, were found in the seeds of line 63. However, seeds of line 91 were superior in regard to vigor. Increased expression of the enzymes SOD and CAT, considered as enzymes of the antioxidant system, was observed in seeds of lines 44 and 91. Thus, line 91 was considered as promising for tolerance to water stress.


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