scholarly journals Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying

2015 ◽  
Vol 37 (2) ◽  
pp. 102-110
Author(s):  
Leandra Helena do Nascimento Gonçalves ◽  
Heloísa Oliveira dos Santos ◽  
Édila Vilela de Resende Von Pinho ◽  
Thaís de Andrade ◽  
Iolanda Vilela Von Pinho ◽  
...  
Keyword(s):  
Gene Expression ◽  
Water Content ◽  
Seed Quality ◽  
Isocitrate Lyase ◽  
Electrophoretic Pattern ◽  
Drying Methods ◽  
Heat Resistant ◽  
Expression Of Genes ◽  
Physiological Quality

Handroanthus serratifolius seeds are considered orthodox and have some mechanisms that assist in desiccation tolerance, like heat-resistant proteins and the presence of protective systems against free radical production. In order to investigate the drying effect on the quality of Handroanthus serratifolius seeds collected with 28% water content two methods were used: slow drying (30 °C) and fast drying (45 °C) until water contents equaled 20%, 15%, 10% and 5%. Seed quality was assessed by germination test, first count of germination, speed germination, emergence and speed emergence. The expression of heat-resistant proteins and enzymes catalase, esterase and isocitrate lyase was assessed by electrophoresis. The gene expression of sHSP17.5, CAT3 and ICL6 was quantified by real time PCR. Higher values of germination and vigor were obtained in seeds with 20% water content, subjected to fast drying. There was induction of heat-resistant proteins expression for both drying methods. There was variation in the electrophoretic pattern of catalase, esterase and isocitrate lyase in seeds subjected to different treatments. The gene expression in Handroanthus serratifolius seeds varies depending on methods of drying and water content of seeds. The drying of Handroanthus serratifolius seeds until the water content between 15 and 10% compromises their physiological quality.

scholarly journals Physiological quality of eggplant seeds with different extraction and drying methods

2013 ◽  
Vol 35 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Leomara Vieira de França ◽  
Mariana Dierings Croda ◽  
Warley Marcos Nascimento ◽  
Raquel Alves de Freitas
Keyword(s):  
Hydrochloric Acid ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Drying Methods ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Seed Extraction ◽  
Physiological Quality

During seed extraction in fleshy fruits, some procedures are necessary to ensure seed quality and minimize deterioration and microorganism activity; also seeds extracted under moist conditions need special care when drying. The objective of this study was to evaluate the physiological quality of eggplant seeds submitted to different extraction and drying methods. In the first study, whole eggplant fruits were mechanically crushed and then fermented for 0, 24 and 48 hours, with and without applying hydrochloric acid during seed extraction. In the second study, seeds were extracted in a pepper peeler and then immediately washed in water and submitted to the following drying methods: Sun/24 h; Sun/48 h; 32 °C/24 h; 32 °C/48 h; 38 °C/24 h; 38 °C/48 h; Sun/24 h + 32 °C/24 h; Sun/24 h + 38 °C/24 h e 32 °C/24 h + 38 °C/24 h. The treatment efficiency of both experiments was evaluated from the following tests: seed weight, germination, first count, accelerated aging and seedling emergence. The results suggest that fermentation and applying hydrochloric acid to pulp/seed reduce the physiological seed quality. All drying methods reduced the seed moisture content permitting adequate storage and maintenance of seed physiological quality.

scholarly journals QUALITY OF NATURAL COFFEE AND PULPED IN THE FUNCTION OF TEMPERATURE ALTERNATION DURING MECHANICAL DRYING

2018 ◽  
Vol 13 (4) ◽  
pp. 415 ◽  
Author(s):  
Pedro Damasceno Oliveira ◽  
Marco Antônio Martin Biaggioni ◽  
Flávio Meira Borém ◽  
Eder Pedroza Isquierdo ◽  
Mariana De Oliveira Vaz Damasceno
Keyword(s):  
Water Content ◽  
Evaluation System ◽  
Drying Methods ◽  
Fixed Layer ◽  
Heated Air ◽  
Coffee Beans ◽  
Specialty Coffee ◽  
Physiological Quality ◽  
Drying System

The research evaluates the sensorial quality of processed and dried coffee beans in different ways. Two types of processing were performed: dry and wet; And seven drying methods: drying in the terrier, and mechanical drying with heated air at 50ºC until coffee reaches 30% water content, followed by drying with air heated to 35ºC until reaching 11% water content; drying in fixed-layer dryers with heated air at 45 ° C until the coffee reaches 30% water content, followed by drying with heated air at 35 ° C until reaching 11% water content; and drying in fixed layer dryers with heated air at 40 ° C until coffee reaches 30% water content, followed by drying with heated air at 35 ° C until reaching 11% water content; drying in fixed layer dryers with heated air at 35 ° C until the coffee reaches 30% water content, followed by drying with heated air at 50 ° C until reaching 11% water content; drying in fixed-layer dryers with heated air at 35 ° C until the coffee reaches 30% water content, followed by drying with heated air at 45 ° C until reaching 11% water content; drying in fixed-layer dryers with heated air at 35 ° C until the coffee reaches 30% water content, followed by drying with heated air at 40 ° C until reaching 11% water content. The mechanical drying system consisted of three dryers of fixed layer, allowing the control of temperature and drying flow. The cafes were tasted according to the evaluation system proposed by the American Specialty Coffee Association (SCAA). Analyzes of the physical-chemical composition and physiological quality of the grains were carried out, involving: acidity grease, potassium leaching, electrical conductivity, color and germination. The results show that the pulped coffee is more tolerant to drying than the natural coffee, regardless of how it was dried.

scholarly journals Gene Expression Related to Physiological Quality of Soybean Seeds

2019 ◽  
Vol 11 (3) ◽  
pp. 370
Author(s):  
Kiliany A. A. Moreno ◽  
Raquel M. O. Pires ◽  
Maria L. R. Castro ◽  
Renato C. C. Vasconcellos ◽  
Heloisa O. Santos ◽  
...  
Keyword(s):  
Gene Expression ◽  
Isocitrate Lyase ◽  
Phosphoglucose Isomerase ◽  
Soybean Seeds ◽  
Plant Populations ◽  
Qrt Pcr ◽  
Study Gene Expression ◽  
Soybean Genotypes ◽  
Physiological Quality

Production of soybeans with high physiological quality is important to ensure the productivity of improved plant populations. The objective of this work was to select soybean genotypes for high physiological quality of seeds, through germination and vigor tests, and to study gene expression by transcripts and proteins. In a first trial, seeds of 12 soybean cultivars were evaluated and selected six cultivars with contrasting physiological quality levels. At the protein level, the isoenzyme systems alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), sorbitol dehydrogenase (SDH), superoxide dismutase (SOD), catalase (CAT), isocitrate lyase (ICL), esterase (EST), glutamate oxaloacetate transferase (GOT), and heat-resistant proteins were evaluated. For the transcript levels, the real-time PCR technique (qRT-PCR) was used, and the genes coding for the SOD, CAT, MDH, PGI, ICL and PRX enzymes were studied. The germination and vigor tests classified the seeds of cultivars CD201, CA115 and MS8400 as high quality, while the cultivars Syn1263, Syn1279 and CD202 were classified as of low quality. The enzymes involved in the process such as dehydrogenase and phosphoglucose isomerase, are promising markers for assessing the physiological quality of soybean seeds. Higher expression of peroxiredoxin enzyme is related to the low physiological quality of soybean seeds.

scholarly journals CHEMICAL DESICCANTS FOR ANTICIPATION OF HARVEST AND PHYSIOLOGICAL QUALITY OF COWPEA SEEDS

2020 ◽  
Vol 33 (4) ◽  
pp. 878-887
Author(s):  
ELIZETH REGINA RAISSE ◽  
MIQUÉIAS DE OLIVEIRA ASSIS ◽  
EDUARDO FONTES ARAUJO ◽  
FRANCISCO CLÁUDIO LOPES DE FREITAS ◽  
ROBERTO FONTES ARAUJO
Keyword(s):  
Seed Germination ◽  
Water Content ◽  
Seed Quality ◽  
Block Design ◽  
Control Treatment ◽  
Controlled Environment ◽  
Glufosinate Ammonium ◽  
Cowpea Seeds ◽  
Physiological Quality

ABSTRACT Information about the effect of herbicides on cowpea seed quality is scarce. The objective of this work was to evaluate the effect of using chemical desiccants at pre-harvest on the anticipation of harvest and physiological quality of cowpea seeds of the cultivar BRS-Tumucumaque, before and after storage. Two experiments were conducted: experiment 1 was conducted under a randomized block design, with four replications; the treatments consisted of seven herbicides (carfentrazone-ethyl, saflufenacil, glyphosate, paraquat, flumioxazin, glufosinate-ammonium, and diquat) and a control treatment; anticipation of harvest, and seed water content, germination, and vigor were evaluated. The seeds were stored for six months in controlled environment and not-controlled environment rooms and evaluated for water content, germination, and vigor in Experiment 2, which was conducted in a 7^2+1 factorial arrangement, with four replications; the first factor consisted of the seven herbicides, the second factor was the two storage environments, plus a control treatment. The results showed 3 to 9 days of anticipation of harvest relative to the control. The glufosinate -ammonium herbicide negatively affected seed germination and vigor. The glyphosate herbicide decreased seed germination and vigor after storage. The physiological quality of cowpea seeds at the post -harvest period and after six months of storage was not affected by the carfentrazone-ethyl and flumioxazin herbicides. Paraquat and diquat were the most efficient herbicides for the anticipation of harvest (nine days). Glyphosate and glufosinate -ammonium were the most harmful herbicides to seed physiological quality. The controlled environment room was the most adequate environment for seed conservation.

scholarly journals The physiological quality of yellow passion fruit (Passiflora edulis Sims. f. flavicarpa Deg.) seeds with different water content placed in a cold chamber room and environmental conditions

2019 ◽  
Vol 13 ((03) 2019) ◽  
pp. 452-457
Author(s):  
Paula Aparecida Muniz de Lima ◽  
Khétrin Silva Maciel ◽  
Rodrigo Sobreira Alexandre ◽  
José Carlos Lopes
Keyword(s):  
Water Content ◽  
Natural Environment ◽  
Seed Quality ◽  
Passion Fruit ◽  
Laboratory Environment ◽  
Physiological Quality ◽  
Cold Chamber ◽  
Natural Laboratory ◽  
Yellow Passion Fruit

Seed quality is extremely important for the crop production system, and storage conditions are important to preserve this quality. The objective this study was to evaluate the physiological quality of yellow passion fruit seeds with different water contents placed in a cold chamber room (4°C) and in a natural laboratory environment (25 ± 2°C) for a period of eight months (240 days). Passion fruit seeds were harvested in orchards of the southern region of Espírito Santo, with water content adjusted to 10%; 14%; 18% and 22%, and placed in a cold chamber room and a natural laboratory environment. Germination, germination speed index, shoot and root length, fresh and dry mass of normal seedlings were evaluated. The experimental design was completely randomized, with two environments (cold chamber room and natural laboratory environment) and with a plot experimental arrangement subdivided according to time, with four replicates of 25 seeds within each environment. Yellow passion fruit seeds placed in a cold chamber room and natural environment with 14% of water content presented a higher physiological quality during eight months of storage. Yellow passion fruit seeds placed in a cold chamber room and natural environment presented maximum germination and vigour with 60 days of storage. Yellow passion fruit seeds exhibit orthodox behaviour, and should be stored at 14% of water content, in a cold chamber room and natural environment, without loss of viability and physiological quality over eight months, presenting maximum germination and vigour after 60 days storage, without the presence of pathogens.

scholarly journals Effects of different drying rates on the physiological quality of Coffea canephora Pierre seeds

2005 ◽  
Vol 17 (2) ◽  
pp. 199-205 ◽  
Author(s):  
Sttela Dellyzete Veiga Franco da Rosa ◽  
Delacyr da Silva Brandão Júnior ◽  
Édila Vilela de Resende Von Pinho ◽  
André Delly Veiga ◽  
Luiz Hildebrando de Castro e Silva
Keyword(s):  
Water Content ◽  
Storage Temperature ◽  
Removal Rate ◽  
Coffea Canephora ◽  
Development Stage ◽  
Seed Vigor ◽  
Drying Rate ◽  
Physiological Quality ◽  
Drying Rates

Desiccation tolerance in seeds depends on the species, development stage and drying conditions, especially the water removal rate. Coffea seeds are considered of intermediate performance, because they tolerate relative dehydration compared to orthodox seeds and are sensitive to low temperatures. The objective of this study was to verify the effect of different drying rates on the viability and storability of Coffea canephora seeds. A complete randomized experimental design was used, in a factorial 3 x 5 x 2 design, with three drying rates (fast, intermediate and slow), five final mean water contents after drying (51, 42, 33, 22 and 15 %) and two storage temperatures (10 and 20°C). The germination and seed vigor assessments, using radicle protrusion, cotyledon leaf opening, seedling emergence and emergence speed index, were performed shortly after drying and after two and four months storage. It was observed that with reduction in the water content there was reduction in the germination values and seed vigor, for all the drying rates. The greatest reductions in physiological quality occurred when the seeds were dried quickly and the best results were obtained at the intermediate drying rate. There was an effect of drying rate and storage temperature on the physiological quality of the seeds, and lower germination and vigor values were observed in seeds with lower water content stored at 20°C. C. canephora seeds were tolerant to desiccation down to 15 % water content and can be stored for four months at 10°C. A temperature of 20ºC can be used to store C. canephora seeds, as long as the water content is not reduced to values below 22 % water content.

scholarly journals Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds

2013 ◽  
Vol 12 (3) ◽  
pp. 3630-3642 ◽  
Author(s):  
T. Andrade ◽  
E.V.R. Von Pinho ◽  
R.G. Von Pinho ◽  
G.E. Oliveira ◽  
V. Andrade ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stages Of Development ◽  
Heat Resistant ◽  
Maize Seeds ◽  
Physiological Quality

scholarly journals Physiological quality of Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds subjected to different storage conditions

2012 ◽  
Vol 36 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Claudinéia Regina Pelacani ◽  
Cimille Gabrielle Cardoso Antunes ◽  
Bárbara França Dantas ◽  
Renato Delmondez de Castro
Keyword(s):  
Water Content ◽  
Seed Viability ◽  
Gliricidia Sepium ◽  
Storage Conditions ◽  
Physiological Processes ◽  
Plastic Bags ◽  
Starting Point ◽  
Cultivated Plant ◽  
Physiological Quality

Studies of seed physiological processes represent the starting point for the sustainable utilization of native or cultivated plant species from the caatinga biome for which germination studies are still scarce. In order to determine adequate methods for the conservation of Gliricidia sepium seeds, the physiological quality of propagules exposed to different conditions and times of storage were examined. Recently collected seeds of G. sepium were analyzed immediately, while the other sample was stored for three, six, nine, or twelve months in plastic or paper bags under refrigeration or at room temperature for further analysis. The germination rates of seeds from each storage regime were determined and these rates compared to their water content. It was observed that germination capacity was directly related to changes in seed humidity. Storing seeds in plastic bags yielded the best conservation, mainly when they were stored at low temperatures. However, even at temperatures near 25°C it was possible to prolong seed viability when their water content was controlled through the use of impermeable packing.

scholarly journals Lignification of the plant and seed quality of RR soybeans sprayed with herbicide glyphosate

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 286-292
Author(s):  
Cristiane Fortes Gris ◽  
Edila Vilela de Resende Von Pinho ◽  
Maria Laene de Moreira Carvalho ◽  
Rafael Parreira Diniz ◽  
Thaís de Andrade
Keyword(s):  
Seed Quality ◽  
Lignin Content ◽  
Block Design ◽  
Accelerated Aging ◽  
Soybean Seeds ◽  
Randomized Block Design ◽  
Physiological Quality ◽  
Hand Weeding ◽  
Herbicide Glyphosate

Differences in levels of lignin in the plant between conventional and transgenic cultivars RR has been reported by several authors, however, there are few studies evaluating the influence of spraying of glyphosate on the lignin in the plant and RR soybean seeds. The aim of this study was to evaluate the physiological quality of RR transgenic soybean seeds and the lignin contents of plants sprayed with the herbicide glyphosate. The assays were conducted both in greenhouse and field in the municipality of Lavras, MG, in the agricultural year 2007/08. The experiment was arranged in a splitplot design with four replicates, considering the treatments hand weeding and herbicide glyphosate as plots, and five RR soybean cultivars (BRS 245 RR, BRS 247 RR, Valiosa RR, Silvânia RR and Baliza RR) as splitplots. In the greenhouse, the cultivars tested were BRS 245 RR and Valiosa RR in a randomized block design with four replicates. The sprayings were carried out at stages V3, V7 and early R5 (3L/ha). The 1000 seed weight, mechanical injury, germination and germination velocity index, emergence velocity index, accelerated aging, electrical conductivity and water soaking seed test, lignin content in the seed coat, in the stem and legumes were determined. The spraying of glyphosate herbicide, in greenhouse and field, did not alter the physiological quality of seeds and the lignin contents in the plant.

scholarly journals Expression of genes SBP and leginsulin in contrasting soybean seed coats

2016 ◽  
Vol 46 (10) ◽  
pp. 1695-1700
Author(s):  
Carlos André Bahry ◽  
Paulo Dejalma Zimmer
Keyword(s):  
Gene Expression ◽  
Seed Quality ◽  
Soybean Seed ◽  
Future Research ◽  
Expression Of Genes ◽  
New Information ◽  
Soybean Genotypes ◽  
Phases Of Development ◽  
Seed Coats ◽  
Early Phases

ABSTRACT: Evaluation of differential candidate gene expression in contrasting soybean seeds is an auxiliary tool in the partial elucidation of processes involved in seeds formation, as well as it contributes to the generation of new information that can be used in future research or in the development of r genetic superior constitutions. The aim of this study was to evaluate the expression of two candidate genes, SBP and leginsulin genes, possibly involved in seed quality, in contrasting coats of four soybean genotypes. Two cultivars of yellow soybeans were used, BMX Potência RR and CD 202, and two lines of black soybean, TP and IAC. Gene expression was evaluated using qPCR in seven stages of development from seed coats for four genotypes, at 25, 30, 35, 40, 45, 50, and 55 days after anthesis. The design was completely randomized, with three replications. Data were subjected to analysis of variance and means compared by Tukey's test at 5% probability. SBP and leginsulin gene have higher expression in the early phases of development from seed coats of BMX Potência RR cultivar, followed by the IAC line. These genotypes are therefore of interest for further research involving these genes.

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