scholarly journals Mechanism of juglone-induced apoptosis of MCF-7 cells by the mitochondrial pathway

2016 ◽  
Vol 15 (3) ◽  
Author(s):  
Y.B. Ji ◽  
G.S. Xin ◽  
Z.Y. Qu ◽  
X. Zou ◽  
M. Yu
Keyword(s):  
Mitochondrial Pathway ◽  
Induced Apoptosis ◽  
Mcf 7

scholarly journals Corn Silk (Zea mays L.) Induced Apoptosis in Human Breast Cancer (MCF-7) Cells via the ROS-Mediated Mitochondrial Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Mai M. Al-Oqail ◽  
Ebtesam S. Al-Sheddi ◽  
Nida N. Farshori ◽  
Shaza M. Al-Massarani ◽  
Eman A. Al-Turki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Caspase 3 ◽  
Mitochondrial Pathway ◽  
Ros Production ◽  
Human Breast ◽  
Caspase 9 ◽  
Corn Silk ◽  
Induced Apoptosis ◽  
Mcf 7

Cancer has been recognized as one of the life-threating diseases. Breast cancer is a leading cause of mortality among women. In spite of current developments in the therapy and diagnosis of cancer, the survival rate is still less. Recently, plant-derived natural products gain attention as anticancer agents due to the nontoxic nature. Therefore, the aim of present study was to investigate the anticancer capacity of corn silk extract (CSE) on human breast cancer (MCF-7) and normal human mesenchymal (hMSC-TERT4) cells. Following 24 h treatment to corn silk extract, the cytotoxicity was assessed by MTT, NRU, and morphological assays. The oxidative stress markers (GSH and LPO), ROS production, MMP change, and expression of apoptotic marker genes (p53, Bax, Bcl-2, caspase-3, and caspase-9) were also studied in MCF-7 cells treated at 250 to 1000 μg/ml of CSE for 24 h. Our results showed that CSE decreased the cell viability and increased the apoptosis in a dose-dependent manner. The level of LPO and ROS production was found significantly higher; however, GSH and MMP level was observed lower in CSE-treated MCF-7 cells. The real-time PCR data showed a significant upregulation in p53, Bax, caspase-3, and caspase-9 and downregulation in the mRNA expression of Bcl-2 genes in MCF-7 cells exposed to CSE. Collectively, the data from this study stated that corn silk extract induced apoptosis via the ROS-mediated mitochondrial pathway in MCF-7 cells.

scholarly journals Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway

2019 ◽  
Vol 20 (19) ◽  
pp. 4803 ◽  
Author(s):  
Shan Jin ◽  
Kuan Shi ◽  
Liu Liu ◽  
Yu Chen ◽  
Guangzhong Yang
Keyword(s):  
Hepg2 Cells ◽  
Chemical Constituents ◽  
Human Tumor ◽  
Mitochondrial Pathway ◽  
Caspase 9 ◽  
Human Tumor Cell Lines ◽  
Proliferation Activity ◽  
Garcinia Xanthochymus ◽  
Induced Apoptosis ◽  
Mcf 7

Xanthones are important chemical constituents of Garcinia xanthochymus and varied bioactivities including cytotoxicity. However, their anti-tumor mechanism has remained unknown. Here, we isolated and identified a new xanthone named garciniaxanthone I (1) and five known compounds from the bark of G. xanthochymus. Their structures were elucidated by NMR analysis and HRESIMS. The anti-proliferation activities of all isolated compounds were evaluated on four human tumor cell lines (HepG2, A549, SGC7901, MCF-7). The results demonstrated that the anti-proliferation activity of xanthone was related to the number and location of prenyl groups. We further found that garciniaxanthone I (GXI) could induce HepG2 apoptosis and enhance the expression of cleaved caspase-8, caspase-9, and caspase-3. GXI could also increase Bax level and concurrently reduce the overexpression of Bcl-2, Bcl-XL, Mcl-1, and surviving in HepG2 cells. Moreover, GXI could inhibit cell migration of HepG2 cells by inhibiting the expressions of MMP-7 and MMP-9. In summary, our study suggests that GXI could induce HepG2 apoptosis via the mitochondrial pathway and might become a lead compound for liver cancer treatment.

Lamotrigine Attenuates Proteasome Inhibition-Induced Apoptosis by Suppressing the Activation of the Mitochondrial Pathway and the Caspase-8- and Bid-Dependent Pathways

2016 ◽  
Vol 41 (10) ◽  
pp. 2503-2516 ◽  
Author(s):  
Yoon Jeong Nam ◽  
Arum Kim ◽  
Min Sung Lee ◽  
Yong Kyoo Shin ◽  
Dong Suep Sohn ◽  
...  
Keyword(s):  
Proteasome Inhibition ◽  
Mitochondrial Pathway ◽  
Caspase 8 ◽  
Induced Apoptosis

Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway

2015 ◽  
Vol 6 (3) ◽  
pp. 765-771 ◽  
Author(s):  
Guidong Huang ◽  
Jian Mao ◽  
Zhongwei Ji ◽  
Aisikaer Ailati
Keyword(s):  
Colon Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Mitochondrial Pathway ◽  
Colon Cancer Cell ◽  
Cancer Cell Growth ◽  
Induced Apoptosis

Some studies have shown that stachyose, as prebiotics, can prevent indirectly colon cancer cell growth by promoting the proliferation of probiotics or producing beneficial materials in the intestine.

ACACIA HONEY INDUCES APOPTOSIS IN HUMAN BREAST ADENOCARCINOMA CELL LINES (MCF-7)

2017 ◽  
Vol 79 (4) ◽  
Author(s):  
Muhammad Ashraf Mohd Salleh ◽  
Zolkapli Eshak ◽  
Wan Iryani Wan Ismail
Keyword(s):  
Antioxidant Properties ◽  
Breast Adenocarcinoma ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Cell Shrinkage ◽  
Cancer Cell Growth ◽  
Breast Cancer Cell Growth ◽  
Acacia Honey ◽  
Induced Apoptosis ◽  
Mcf 7

Apoptosis is one of markers considered in drug design including in treating cancers. Conventional treatments for cancer cause various side effects. One of possible alternatives is honey, an antioxidant driven by its phenolic and flavonoids content. This study aims to observe the effects of Malaysian Acacia honey in inhibiting breast cancer cell growth through apoptosis. Antioxidant properties of the honey were measured using total phenolic content (TPC) and total flavonoid content (TFC). Then, antiproliferative activity of the honey were observed using MTT assay. The honey exhibited IC50 at concentration of 5.5% (v/v). Further studies using TUNEL and live cell view revealed that Acacia honey induced apoptosis after 6 hours of treatment. Cell shrinkage, which is one of the apoptotic features, was observed as early as 2 hours, followed by the formation of apoptotic bodies within 6 hours of the honey treatment. Details of mechanism and actual compounds involved in displaying the results are being intensively studied.

Gossypol induced apoptosis of polymorphonuclear leukocytes and monocytes: Involvement of mitochondrial pathway and reactive oxygen species

2009 ◽  
Vol 31 (2) ◽  
pp. 320-330 ◽  
Author(s):  
Martha Barba-Barajas ◽  
Georgina Hernández-Flores ◽  
José M. Lerma-Díaz ◽  
Pablo C. Ortiz-Lazareno ◽  
Jorge R. Domínguez-Rodríguez ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Polymorphonuclear Leukocytes ◽  
Reactive Oxygen ◽  
Mitochondrial Pathway ◽  
Oxygen Species ◽  
Induced Apoptosis
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