scholarly journals Molecular Detection and Typing of Pathogenic Leptospira in Febrile Patients and Phylogenetic Comparison with Leptospira Detected among Animals in Tanzania

2020 ◽  
Vol 103 (4) ◽  
pp. 1427-1434
Author(s):  
Kathryn J. Allan ◽  
Michael J. Maze ◽  
Renee L. Galloway ◽  
Matthew P. Rubach ◽  
Holly M. Biggs ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Pathogenic Leptospira ◽  
Phylogenetic Comparison

scholarly journals Molecular characterisation of Leptospira strains in Pakistan

2016 ◽  
Vol 60 (4) ◽  
pp. 417-421
Author(s):  
Muhammad Luqman Sohail ◽  
Muhammad Sarwar Khan ◽  
Muhammad Avais ◽  
Muhammad Yasir Zahoor ◽  
Irfan Khattak ◽  
...  
Keyword(s):  
16S Rrna ◽  
Animal Species ◽  
Molecular Detection ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Intermediate Species ◽  
Specific Primers ◽  
Pathogenic Leptospira ◽  
Wide Range ◽  
Serological Studies

Abstract Introduction: Leptospirosis affects a wide range of mammals, humans, and even a few poikilothermic animal species. In Pakistan, serological studies of equine leptospirosis have reported a prevalence of over 40%, but no study has ever been conducted towards molecular detection of Leptospira in horses. Material and Methods: Blood samples from 128 horses were screened using ELISA and 41 positive samples were examined for the presence of leptospiral DNA using specific primers for 16S rRNA gene. Results: Out of 41 tested samples, 20 samples were found to be PCR-positive, revealing a fragment of 306 bp after gel electrophoresis. Sequencing and phylogenetic analysis of positive samples revealed circulation of pathogenic Leptospira spp. in Pakistani horses. No evidence of circulation of intermediate species was found in this study. Conclusion: This study reports the first molecular evidence of equine leptospirosis in Pakistan and lays ground for further research in this area. It also confirms the efficiency of 16S rRNA for the diagnosis of equine leptospirosis.

scholarly journals Molecular detection and isolation of pathogenic Leptospira from asymptomatic humans, domestic animals and water sources in Nan province, a rural area of Thailand

2017 ◽  
Vol 115 ◽  
pp. 146-154 ◽  
Author(s):  
Alongkorn Kurilung ◽  
Pattrarat Chanchaithong ◽  
Kittitat Lugsomya ◽  
Waree Niyomtham ◽  
Vanaporn Wuthiekanun ◽  
...  
Keyword(s):  
Rural Area ◽  
Molecular Detection ◽  
Domestic Animals ◽  
Water Sources ◽  
Pathogenic Leptospira

scholarly journals Tracking animal reservoirs of pathogenic Leptospira: the right test for the right claim.

2021 ◽  
Author(s):  
Yann Gomard ◽  
Koussay Mohamed Dellagi ◽  
Steven Goodman ◽  
Patrick Mavingui ◽  
Pablo Tortosa
Keyword(s):  
Chronic Infection ◽  
One Health ◽  
Molecular Detection ◽  
Published Data ◽  
Pathogenic Leptospira ◽  
Human Leptospirosis ◽  
Previous Infection ◽  
Serological Data ◽  
The Right ◽  
Animal Reservoirs

Leptospirosis, caused by a pathogenic Leptospira bacteria, is the most prevalent zoonosis worldwide and in this context has been extensively investigated through a One Health framework. Diagnosis of human leptospirosis includes molecular and serological tools, with serological Microscopic Agglutination Test (MAT) still being considered as a gold standard. Mammals considered as biological reservoirs include species or populations that are able to maintain chronic infection and shed the bacteria via their urine in the environment. Leptospira bacteria are often investigated using the same diagnosis tool, serological MAT. However, MAT testing of putative animal reservoirs can lead to mis-interpretations as it can signal previous infection and not necessarily bring in robust information regarding the capacity of such sero-positive animals to maintain chronic infection. We use previously published data and present new results on introduced and endemic small mammals to show that MAT should not be used for the identification of reservoirs. By contrast, serological data are informative on the level of exposure of animals occupying a specific environment. Finally, we present a sequential methodology to investigate human leptospirosis in a One Health framework that associates molecular detection in humans and animals, together with MAT of human samples using Leptospira isolates obtained from reservoir animals occurring in the same environment.

Molecular Detection of Pathogenic Leptospira in Grasscutters (Thryonomys swinderianus) from Côte d'Ivoire

2020 ◽  
Vol 20 (12) ◽  
pp. 924-926
Author(s):  
Nassim Hammoudi ◽  
Bernard Davoust ◽  
Sylvestre Dizoe ◽  
Marine Le Guyader ◽  
Michel Drancourt ◽  
...  
Keyword(s):  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Molecular Detection ◽  
Pathogenic Leptospira ◽  
Cote D’Ivoire ◽  
Thryonomys Swinderianus ◽  
Côte D'ivoire

Changes in the Population Density of Pathogenic Microorganisms in Response to the Allelopathic Effect of Thypha Latifolia

2014 ◽  
Vol 1 (1) ◽  
pp. 31-36 ◽  
Author(s):  
O. Zhukorskiy ◽  
O. Gulay ◽  
V. Gulay ◽  
N. Tkachuk
Keyword(s):  
Cell Density ◽  
Allelopathic Effect ◽  
Pathogenic Microorganisms ◽  
Sterile Water ◽  
Erysipelothrix Rhusiopathiae ◽  
Pathogenic Leptospira ◽  
Favorable Conditions ◽  
And Control ◽  
The Impact ◽  
Control Samples

Aim. To determine the response of the populations of Erysipelothrix rhusiopathiae and Leptospira interrogans pathogenic microorganisms to the impact of broadleaf cattail (Thypha latifolia) root diffusates. Methods. Aqueous solutions of T. latifolia root diffusates were sterilized by vacuum fi ltration through the fi lters with 0.2-micron pore diameter. The experimental samples contained cattail secretions, sterile water, and cultures of E. rhusiopathiae or L. interrogans. The same amount of sterile water, as in the experimental samples, was used for the purpose of control, and the same quantity of microbial cultures was added in it. After exposure, the density of cells in the experimental and control samples was determined. Results. Root diffusates of T. latifolia caused an increase in cell density in the populations of E. rhusiopathiae throughout the whole range of the studied dilutions (1:10–1:10000). In the populations of the 6 studied serological variants of L. interrogans spirochetes (pomona, grippotyphosa, copenhageni, kabura, tarassovi, canicola), the action of broadleaf cattail root diffusates caused the decrease in cell density. A stimulatory effect was marked in the experimental samples of the pollonica serological variant of leptospira. Conclusions. The populations of E. rhusiopathiae and L. interrogans pathogenic microorganisms respond to the allelopathic effect of Thypha latifolia by changing the cell density. The obtained results provide the background to assume that broadleaf cattail thickets create favorable conditions for the existence of E. rhusiopathiae pathogen bacteria. The reduced cell density of L. interrogans in the experimental samples compared to the control samples observed under the infl uence of T. latifolia root diffusates suggests that reservoirs with broadleaf cattail thickets are marked by the unfavorable conditions for the existence of pathogenic leptospira (except L. pollonica).

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