scholarly journals Spatiotemporal Heterogeneity of Zika Virus Transmission in Indonesia: Serosurveillance Data from a Pediatric Population

2021 ◽  
Author(s):  
R. Tedjo Sasmono ◽  
Edison Johar ◽  
Benediktus Yohan ◽  
Chairin Nisa Ma’roef ◽  
Paul Pronyk ◽  
...  
Keyword(s):  
Geographic Distribution ◽  
Zika Virus ◽  
Pediatric Population ◽  
Age Groups ◽  
Virus Transmission ◽  
Transmission Dynamics ◽  
Serum Samples ◽  
Serological Tests ◽  
Asymptomatic Children ◽  
Geographic Differences

The presence of Zika virus (ZIKV) in Indonesia has been recognized since the 1970s, but its transmission dynamics there have been poorly understood. To understand more fully the geographic distribution and burden of ZIKV disease, we performed retrospective serological tests on specimens collected from asymptomatic children age 5 to 9 years old living at 30 sites in 14 provinces. Of 870 serum samples tested, 9.2% were found to be positive for anti-ZIKV antibodies, as confirmed by plaque reduction neutralization assays. This was the same overall prevalence reported previously for 1- to 4-year-old children collected at the same sites at the same time. Together with geographic differences in seroprevalence between the age groups, these data suggest that, although ZIKV might be endemic in Indonesia, its occurrence has been focal and episodic.

scholarly journals Seroprevalance of Toxoplasmosis in sheep and goat: Iraq/ Sulaimania

2011 ◽  
Vol 35 (1) ◽  
pp. 16-24
Author(s):  
Lazem H. Al-Taie
Keyword(s):  
Age Groups ◽  
Neural System ◽  
Farm Animals ◽  
Economic Losses ◽  
Age Group ◽  
Serum Samples ◽  
Serological Tests ◽  
Back Ground ◽  
Qualitative Determination

Back ground: Toxoplasmosis is an important zoonosis that causes economic losses in animal herds due to abortion and stillbirth as well as changes in the reproductive and neural system of susceptible animals . Objective: The aims of the present study is to determination the prevalence of T. gondii in farm animals ( sheep& goat)of both genders and different ages in Sulaimani province by using two serological tests (ELISA and LAT). Methods: Blood samples were collected from farm animals ,142 sheep and 46 goats , of different sexes and ages. Tow different serological tests ,ELISA and LAT for qualitative determination of T. gondii antibody titer in sheep and goats serum samples. Results: The prevalence rate in sheep was 73 (51.7 %) and 82 (57 %) , and 21 (54.6 %) and 25 (54.35 %) in goats ,by ELISA and LAT respectively. The prevalence of toxoplasmosis was highest in age group 7-9 (66.6%) in sheep in compares’ with other age groups. There was no significant differences between both spp.and tow test. Conclusion: Statistical results show no significant differences between both tests (ELISA &LAT) at (P ≥ 0.05).The prevalence of toxoplasmosis was increased proportionally with the age of animals, while gender has no effect on the prevalent rate .

scholarly journals Quantitative serological evaluation as a valuable tool in the COVID-19 vaccination campaign

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Davide Ferrari ◽  
Alessandra Mangia ◽  
Maria Sestina Spanò ◽  
Lucia Zaffarano ◽  
Marco Viganò ◽  
...  
Keyword(s):  
Immune Response ◽  
General Population ◽  
Serological Test ◽  
Vaccination Campaign ◽  
Age Groups ◽  
Vaccine Dose ◽  
Serum Samples ◽  
Serological Tests ◽  
Antibody Persistence ◽  
Definition Of

Abstract Objectives After exceptional research efforts, several vaccines were developed against SARS-CoV-2 which sustains the pandemic COVID-19. The Comirnaty vaccine showed high efficacy in clinical trials and was the first to be approved for its distribution to the general population. We evaluated the immune response induced by the first vaccine dose in different sex/age groups and subjects with or without naturally present anti-SARS-CoV-2 antibodies. Methods As part of an Italian multicenter project (Covidiagnostix), serum samples from 4,290 health-professionals were serologically tested the day of the first vaccination dose, and 21 days later, using two different instrumentations (Siemens-Healthineers and Roche). Results In total, 97% of samples showed the presence of specific antibodies 21 days after the vaccination dose; the percentage of non-responders increased with age in both genders. Remarkably, naturally seropositive individuals showed antibody persistence up to 11 months and an exceptionally higher vaccination response compared to subjects never infected by SARS-CoV-2. Conclusions This study highlighted the importance of the serological test i) to identify naturally SARS-CoV-2 seropositive individuals and ii) to evaluate the antibody level elicited by the first vaccination dose. Both tests, highlighted differences in the immune response, when subjects were stratified by sex and age, and between naturally seropositive and seronegative subjects. The data obtained show how serological tests could play a crucial role in the triage of the population subjected to the vaccination campaign for COVID-19. The definition of suitable instrumentation-specific thresholds is needed to correctly follow eventually acquired post-vaccination immunity in the general population.

scholarly journals Age- and sex-specific reference intervals for the serum cystatin C/creatinine ratio in healthy children (0–18 years old)

2019 ◽  
Vol 47 (7) ◽  
pp. 3151-3159 ◽  
Author(s):  
Changjin Liu ◽  
Jing Wen ◽  
Jialin Xiang ◽  
Xuhong Ouyang ◽  
Yan Yang ◽  
...  
Keyword(s):  
Cystatin C ◽  
Pediatric Population ◽  
Age Groups ◽  
Serum Markers ◽  
Reference Intervals ◽  
Specific Reference ◽  
Healthy Children ◽  
Serum Samples ◽  
Serum Cystatin C ◽  
Age And Sex

Objective This study aimed to investigate serum levels of the cystatin C (CysC)/creatinine (Cr) ratio and renal serum markers (CysC, Cr, urea, and uric acid [UA]) for different ages and by sex. We also aimed to establish pediatric reference intervals for the serum CysC/Cr ratio. Methods Serum samples were collected from 4765 healthy children (0–18 years old). Serum markers of renal function were measured, and the CysC/Cr ratio of each participant was calculated and statistically analyzed. Results The renal marker CysC did not substantially change after 1 year old. Cr, urea, and UA levels generally increased with age. However, the serum CysC/Cr ratio steadily decreased with age. The CysC/Cr ratio showed significant differences in age among all age groups and varied with sex, except for in the 1 to 6-year-old groups. The overall serum CysC/Cr ratio in girls was higher than that in boys. Conclusion Reference intervals of the serum CysC/Cr ratio in the pediatric population were established. These intervals need to be partitioned by age and sex.

scholarly journals Lawsonia intracellularis seroprevalence among the pig farm livestock of Ukraine

2020 ◽  
Vol 8 (4) ◽  
pp. 261-264
Author(s):  
A. N. Ermolenko ◽  
E. E. Aуshpur ◽  
I. U. Mushtuk ◽  
V. V. Gumeniuk ◽  
V. V. Ukhovskyi ◽  
...  
Keyword(s):  
Age Groups ◽  
Well Being ◽  
Test System ◽  
Growing Pigs ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lawsonia Intracellularis ◽  
Serum Samples ◽  
Serological Tests ◽  
Elisa Testing ◽  
Pig Farms

To study the seroprevalence of Lawsonia intracellularis among the pig livestock of Ukraine. The research was conducted in the laboratories of the Institute of Veterinary Medicine of NAAS and in pig farms. Were studied 653 samples of pigs’ serum of different age groups from17 pig farms with breeding stock of 40 456 sows. To study seroprevalence was used a test system for enzyme-linked immunosorbent assay based on monoclonal antibodies bioScreen Ileitis Antibody Test ELISA Svanovir®Lawsonia intracellularis (Boehringer Ingelheim Svanova). Serological tests have provided evidence that the pathogen was circulating in the herd. According to the research results, 46.4% of seropositive animals were found. Seropositive pigs were found in all studied farms, namely – among sows – 74.0%, replacement gilts – 79.6%, growing pigs – 2.5% and fattening pigs – 59.8%. The high level of seroprevalence among replacement gilts and sows of the 1st farrowing indicates the circulation of the pathogen in the herd, as well as the manifestation of morbidity in the fattening group, where was the highest rate of positive serum samples. From the 17 examined farms, only one farm was found where no seropositive animals were detected. According to our observations, proliferative enteropathy in pigs of Ukrainian pig farms has signs of chronic course, especially it is common in pig farms that import replacement gilts from abroad, where antibiotics are prohibited or restricted in donor farms. The results of serological tests allowed us to reconfirm that the spread of the disease is increasing in Ukrainian pig farms. Compared to 2015, the percentage of seropositive animals increased by almost 10%. The typical serological profile for Lawsonia intracellularis which is characteristic of our studied farms allowed us to conclude for which age groups of pigs and for which technological period of raising swine it is necessary to develop and implement measures for treatment and disease prevention. To control the disease, it is highly recommended to conduct constant monitoring of the farm’s well-being against ileitis (send, in addition to faecal samples for PCR testing, also serum for ELISA testing).

scholarly journals Modeling Zika Virus Transmission Dynamics: Parameter Estimates, Disease Characteristics, and Prevention

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Munsur Rahman ◽  
Kidist Bekele-Maxwell ◽  
LeAnna L. Cates ◽  
H. T. Banks ◽  
Naveen K. Vaidya
Keyword(s):  
Zika Virus ◽  
Virus Transmission ◽  
Transmission Dynamics ◽  
Parameter Estimates ◽  
Disease Characteristics

scholarly journals Total and Exchangeable Copper Assay Using Inductively Coupled Plasma Mass Spectrometry and Establishment of a Pediatric Reference Interval

2020 ◽  
Author(s):  
Jisook Yim ◽  
Soo Beom Kwon ◽  
Jung Sun Han ◽  
Jeong-Ho Kim ◽  
Eun Hee Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Pediatric Population ◽  
Age Groups ◽  
Reference Interval ◽  
Reference Intervals ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Total Copper ◽  
Plasma Mass Spectrometry

Context.— Recently, an exchangeable copper (CuEXC) assay has been suggested as a robust and feasible diagnostic tool for Wilson disease (WD). Although WD is a disorder that requires lifelong treatment and monitoring, few data are currently available regarding the status of copper levels in children. Objective.— To evaluate the performance of copper assays and establish a reference interval for total copper and CuEXC in the pediatric population. Design.— Serum samples from children aged 1–5 (n = 122), 6–12 (n = 125), and 13–18 years (n = 120) were analyzed. Total copper and CuEXC concentrations were directly measured using inductively coupled plasma mass spectrometry, and relative CuEXC levels were calculated. Total copper reference intervals, CuEXC levels, and relative CuEXC levels were determined based on the 2.5th and 97.5th percentiles of the data with 90% confidence intervals. Results.— There were significant differences in the median concentrations of total copper and relative CuEXC among the age groups. Reference intervals determined for total copper were 82 to 167, 75 to 139, and 64 to 133 μg/dL for children aged 1 to 5, 6 to 12, and 13 to 18 years, respectively. The reference intervals for CuEXC were 4.29 to 9.79, 4.02 to 9.09, and 3.55 to 8.25 μg/dL for children aged 1 to 5, 6 to 12, and 13 to 18 years, respectively. Among 11 patients with suspected WD, relative CuEXC values were elevated in all 3 diagnosed with WD. Conclusions.— The pediatric reference intervals derived in this study are expected to be useful for the diagnosis, differential diagnosis, treatment, and monitoring of pediatric patients with WD.

scholarly journals Viral genome-based Zika virus transmission dynamics in a paediatric cohort during the 2016 Nicaragua epidemic

EBioMedicine ◽  
2021 ◽  
Vol 72 ◽  
pp. 103596
Author(s):  
Haoyang Sun ◽  
Raquel A. Binder ◽  
Borame Dickens ◽  
Paola Florez de Sessions ◽  
Maia A. Rabaa ◽  
...  
Keyword(s):  
Zika Virus ◽  
Viral Genome ◽  
Virus Transmission ◽  
Transmission Dynamics ◽  
Paediatric Cohort

scholarly journals An autochthonous case of Zika due to possible sexual transmission, Florence, Italy, 2014

2016 ◽  
Vol 21 (8) ◽  
Author(s):  
Giulietta Venturi ◽  
Lorenzo Zammarchi ◽  
Claudia Fortuna ◽  
Maria Elena Remoli ◽  
Eleonora Benedetti ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Sexual Transmission ◽  
Serum Samples ◽  
Serological Tests ◽  
Autochthonous Case ◽  
Zika Virus Infection

We report a case of Zika virus infection imported in Florence, Italy ex-Thailand, leading to a secondary autochthonous case, probably through sexual transmission. The two cases occurred in May 2014 but were retrospectively diagnosed in 2016 on the basis of serological tests (plaque reduction neutralisation) performed on stored serum samples. Our report provides further evidence that sexual transmission of Zika virus is possible.

Increase in Reported Prevalence of Microcephaly in Infants Born to Women Living in Areas with Confirmed Zika Virus Transmission During the First Trimester of Pregnancy — Brazil, 2015

2016 ◽  
Vol 65 (9) ◽  
Author(s):  
Wanderson Kleber de Oliveira ◽  
Juan Cortez-Escalante ◽  
Wanessa Tenório Gonçalves Holanda De Oliveira ◽  
Greice Madeleine Ikeda do Carmo ◽  
Cláudio Maierovitch Pessanha Henriques ◽  
...  
Keyword(s):  
Zika Virus ◽  
Virus Transmission ◽  
First Trimester ◽  
First Trimester Of Pregnancy
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