Measuring Glycosylated Haemoglobin (HBA1C): A Comparison of Boronate Affinity Chromatographic Method and Spectrophotometric Methods

2011 ◽  
Vol 10 (1) ◽  
Author(s):  
JO Idemudia ◽  
EO Onovughakpo-Sakpa ◽  
E Ayinbuomwan ◽  
BE Kasia
Keyword(s):  
Chromatographic Method ◽  
Glycosylated Haemoglobin ◽  
Spectrophotometric Methods

scholarly journals Analyses of Antioxidative Properties of Selected Cyclitols and Their Mixtures with Flavanones and Glutathione

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 158
Author(s):  
Joanna Płonka ◽  
Joanna Szablińska-Piernik ◽  
Bogusław Buszewski ◽  
Irena Baranowska ◽  
Lesław B. Lahuta
Keyword(s):  
Reactive Oxygen Species ◽  
Chromatographic Method ◽  
Antioxidant Properties ◽  
Ros Generation ◽  
Oxygen Species ◽  
Dpph Radical ◽  
Antioxidative Properties ◽  
Spectrophotometric Methods ◽  
First Time ◽  
Derivatives Of

The conditions for determining the antioxidant properties of cyclitols (d-pinitol, l-quebrachitol, myo-, l-chiro-, and d-chiro-inositol), selected flavanones (hesperetin, naringenin, eriodictyol, and liquiritigenin) and glutathione by spectrophotometric methods—CUPRAC and with DPPH radical, and by a chromatographic method DPPH-UHPLC-UV, have been identified. Interactions of the tested compounds and their impact on the ox-red properties were investigated. The RSA (%) of the compounds tested was determined. Very low antioxidative properties of cyclitols, compared with flavanones and glutathione alone, were revealed. However, a significant increase in the determined antioxidative properties of glutathione by methyl-ether derivatives of cyclitols (d-pinitol and l-quebrachitol) was demonstrated for the first time. Thus, cyclitols seem to be a good candidate for creating drugs for the treatment of many diseases associated with reactive oxygen species (ROS) generation.

scholarly journals Evaluation of the caseinomacropeptide (CMP) content in raw and uht milk during storage time.

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Elaine Closs ◽  
Claucia Fernanda Volken de Souza
Keyword(s):  
Storage Time ◽  
Chromatographic Method ◽  
Correlation Coefficients ◽  
Storage Period ◽  
Raw Milk ◽  
Bacteria Growth ◽  
Uht Milk ◽  
Psychrotrophic Bacteria ◽  
Spectrophotometric Methods

<p>Coolers are used in raw milk storage in order to inhibit the growth of lactic acid producer mesophilic microorganisms. However, refrigerated milk is a favorable environment to psychrotrophic aerobic bacteria growth. These psychrotrophic bacteria promote proteolysis which is of concern to the dairy industry since it may indicate a false positive of fraudulent addition of whey to raw milk. This study evaluated the influence of storage time on the presence of caseinmacropeptide (CMP) in raw milk during storage as well as in ultra-high temperature milk (UHT) throughout its shelf life. Three different determination methods were compared each other, namely the standard chromatographic method and two spectroscopy methods for the quantitative determination of sialic acid. One is based on the determination of ninhydrin acid and the other one is an adapted method using the Ehrlich reagent. An increase of CMP content throughout the storage period for the cooled raw milk and UHT milk was detected despite the fact that none had cheese whey. The spectrophotometric methods showed correlation coefficients greater than 0.97 with the chromatographic method established by Brazilian legislation. The results indicate that the spectrophotometric method using the ninhydrin acid can be used as an alternative method for the determination of CMP in milk samples.</p><p>&nbsp;</p><p>DOI: http://dx.doi.org/10.14685/rebrapa.v2i2.54</p>

scholarly journals FREE GLYCERIN DETERMINATION IN ETHANOL BIODIESEL UTILIZING SPECTROPHOTOMETRIC AND CHROMATOGRAPHIC (GC/FID) METHODS

2017 ◽  
Vol 38 (1) ◽  
pp. 25
Author(s):  
Hilton C. Louzeiro ◽  
Luiz E. B. Soledade ◽  
Antonio G. Souza ◽  
Adeilton P. Maciel ◽  
Fernando C. Silva
Keyword(s):  
Natural Gas ◽  
Present Method ◽  
Chromatographic Method ◽  
Methyl Esters ◽  
Detection Limits ◽  
Ethyl Esters ◽  
National Agency ◽  
Spectrophotometric Methods ◽  
Gas Chromatographic Method

According to the Resolution number 7 from the Brazilian National Agency of Oil, Natural Gas and Biofuels (ANP), the official methodologies for free glycerin determination in biodiesel are the ASTM D 6584, EN 14105 and EN 14106 methods. However, these procedures are limited to the analysis of free glycerin in methyl esters and they are not suitable for the analysis of esters from lauric oils. In the present work, a gas chromatographic method was developed for the determination of the amount of free glycerin in ethyl esters from lauric oils, aiming at overcoming the limitations present in the official methods. Moreover, the present method can also be used for ethanol and methanol biodiesel samples from other oily sources. Besides, a methodology that ascertains the content of free glycerin by UV-Vis spectrophotometry was also applied. Both methods were sensitive enough to determine the content of free glycerin in biodiesel, below the limit specified by the ANP, fixed at 0.02 % (200 mg/L), achieving the detection limits of 5.69 mg/L and 2.17 mg/L for the chromatographic and spectrophotometric methods, respectively.

Determination of DDVP in DDVP-Dipterex Formulations by Gas Chromatography

1965 ◽  
Vol 48 (6) ◽  
pp. 1115-1117
Author(s):  
John Ellis ◽  
John Bates
Keyword(s):  
Gas Chromatography ◽  
Electron Capture ◽  
Chromatographic Method ◽  
Spectrophotometric Methods ◽  
Other Substances ◽  
Gas Chromatographic Method

Abstract In the analyses of commercial fly-bait samples for DDVP (0,0-dimethyI-2,2-dichloro vinyl phosphate), containing Dipterex (0,0-dimethyl-2,2,2-trichloro - 1 - hydroxyethyl phosphonate) as a guaranteed ingredient, interferences were observed at 9.9– 10.4/t with the current spectrophotometric methods. These samples were analyzed by an electron capture gas chromatographic method, and the results showed no interference from Dipterex or other substances. A selected group of samples was analyzed by gas chromatography and spectrophotometry, and the results were compared.

scholarly journals Practical Implication of Chromatographic Method for Estimation of Aceclofenac and Pregabalin in Bulk and Pharmaceutical Dosage Forms

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Hitendrakumar D. Gelani ◽  
Payal P. Chauhan ◽  
Samir K. Shah
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
Practical Implication ◽  
High Sensitivity ◽  
High Performance Liquid Chromatographic ◽  
Uv Detector ◽  
Pharmaceutical Dosage Forms ◽  
Spectrophotometric Methods ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic

Background. Aceclofenac and Pregabalin in combination significantly reduce pain as compared to individual drug in chronic low back pain. Literature reveals that all the reported spectrophotometric methods either need tedious extraction procedures, do not offer high sensitivity, use nonspecific reagent, or recommend the measurement of absorbance in the near UV region where interference most probably occurs that does not offer suitable linearity range. Result. A selective, sensitive, accurate, and precise, high performance liquid chromatographic method with UV detector analysis of Aceclofenac and Pregabalin was investigated. Good chromatographic separation was achieved using an ODS-BP hypersil C18 column (250 mm × 4.6 mm, i.d., 5 μm) and a mobile phase consisting of 0.05 M phosphate buffer (KH2PO4) (pH 6.0) : methanol (60 : 40, v/v) at a flow rate 1 mL/min. The ultraviolet detector was set at wavelength 218 nm. Retention time for Aceclofenac and Pregabalin was found to be 3.220 and 5.910 min, respectively. Rectilinear relationship with good regression coefficients 0.999 and 0.999 was found over the concentration ranges of 5–25 μg/mL and 3.75–18.75 μg/mL for ACF and PGB, respectively, with detection limits 0.64 and 0.35 μg/mL and quantitation limits 1.95 and 1.06 μg/mL. Conclusion. The mean percentage recoveries were in the range of 98.45–100.08 and 99.69–100.48 for ACF and PGB, respectively. The developed method was successfully applied to the analysis of the drugs in their commercial tablets.

Platelet Behaviour in Non-Insulin-Dependent Diabetes -Influence of Vascular Complications, Treatment and Metabolic Control

1986 ◽  
Vol 55 (03) ◽  
pp. 361-365 ◽  
Author(s):  
I Peacock ◽  
M Hawkins ◽  
S Heptinstall
Keyword(s):  
Blood Glucose ◽  
Metabolic Control ◽  
Platelet Reactivity ◽  
Platelet Rich Plasma ◽  
Vascular Complications ◽  
Adenosine Diphosphate ◽  
Glycosylated Haemoglobin ◽  
Insulin Dependent Diabetes ◽  
Lipid Levels ◽  
Insulin Dependent

SummaryPlatelet-rich plasma was prepared from 47 patients with noninsulin-dependent diabetes treated with glibenclamide and metformin, and 21 controls. The release of radio-labelled 5-hydroxy-tryptamine in response to aggregating agents (adenosine diphosphate, adrenaline and sodium arachidonate), and the effects on release of a selective thromboxane inhibitor (UK-34787) were investigated. Subsequently, 20 of the diabetic subjects were chosen at random for treatment with insulin; the remainder continued to take tablets. Platelet studies were then repeated, in all patients, after 4 and 6 months.The results showed an association between platelet behaviour and the presence of vascular complications, and were consistent with previous observations of reduced platelet reactivity in patients taking sulphonylureas. There was no correlation of platelet reactivity with blood glucose, glycosylated haemoglobin or lipid levels.

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