Pumpkin seed oil alleviates oxidative stress and liver damage induced by sodium nitrate in adult rats: biochemical and histological approach

Background: Nitrate (NO3) is the most common chemical contaminant in the world’s ground water aquifer. Oxidative stress has been proposed as a possible mechanism involved in NO3 toxicity on non-target organism. Objectives: The current study aimed to elucidate the potential protective effect of Telfairia occidentalis (pumpkin seed oil, PSO) against hepatotoxicity induced by sodium nitrate. Methods: Wistar rats were exposed either to NaNO3 (200 mg/kg bw) in drinking water in drinking water, or to 4ml PSO/kg bw by gavage or to their combination. Oxidative stress parameters, biochemical biomarkers and liver histopathological examination were determined. Results: Our data showed that the exposure of rats to NaNO3 caused significant changes of some haematological parameters compared to the control. In addition, there was a significant elevation of the levels of biochemical markers as that of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and lactate dehydrogenase when compared with the control. Furthermore, exposure of rats to NaNO3 induced liver oxidative stress as indicated by the increase of malondialdehyde, pro- gressive oxidation of protein products and protein carbonyl levels. In addition, a reduction in anti-oxidant status (catalase, glutathione peroxidase, glutathione-S-transferase and superoxide dismutase, reduced glutathione and vitamin C) was observed. Conclusion: Co-administration of PSO to the NaNO3 restored most parameters cited above to near-normal values. Therefore, the present investigation revealed the ability of PSO to attenuate NaNO3-induced oxidative damage. Keywords: Sodium nitrate; Telfairia occidentalis; pumpkin seed oil; hepatotoxicity; oxidative stress; protection; anti-oxidant status, rat.

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Schistosomicidal, antifibrotic and antioxidant effects of Cucurbita pepo L. seed oil and praziquantel combined treatment for Schistosoma mansoni infection in a mouse model

Journal of Helminthology ◽

10.1017/s0022149x18000317 ◽

2018 ◽

Vol 93 (3) ◽

pp. 286-294 ◽

Cited By ~ 3

Author(s):

E.V.N. Beshay ◽

A.A. Rady ◽

A.F. Afifi ◽

A.H. Mohamed

Keyword(s):

Oxidative Stress ◽

Oral Dose ◽

Schistosoma Mansoni ◽

Post Infection ◽

Cucurbita Pepo ◽

Seed Oil ◽

Histopathological Examination ◽

Combined Treatment ◽

Egg Load ◽

Pumpkin Seed

AbstractDespite the seriousness of schistosomiasis, its treatment depends only on praziquantel (PZQ), which has begun to lose its efficacy against the emergent Schistosoma mansoni-resistant strains. Therefore, the discovery of a novel schistosomicidal drug is an urgent priority. This study was designed to evaluate treatment with Cucurbita pepo L. (pumpkin) seed oil (PSO) alone and combined with PZQ against S. mansoni in experimentally infected mice. The study involved five groups: GI was the normal control; GII was the infected control; GIII was treated with an oral dose of PZQ of 500 mg/kg/day for two successive days, starting in the sixth week post infection; GIV was treated with an oral dose of PSO of 50 mg/kg/day for four weeks, starting in the fourth week post infection; and GV was treated with combined PSO–PZQ. Worm burden, tissue egg load and oogram pattern were estimated, and the ultrastructure alterations were examined. Histopathological examination of granuloma diameters, collagen deposition (Picro Sirius red stain), and angiogenesis (immunohistochemical expression of CD34+) was conducted and serum liver enzymes were measured to assess the liver condition. Moreover, the oxidative stress was evaluated by determining the amounts of malondialdehyde and superoxide dismutase in liver homogenates. The results revealed significant changes in all the assessed parameters with PSO administration. However, PZQ was significantly more effective as an antiparasitic agent, whereas PSO was better in terms of fibrosis and oxidative stress. The most significant results were obtained in group V, which may be attributed to a synergy between PZQ and PSO, with antiparasitic, antioxidant and antifibrotic properties.

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Pumpkin seed oil and zinc attenuate chronic mild stress perturbations in the cerebral cortex of rats

Nutrition & Food Science ◽

10.1108/nfs-10-2021-0315 ◽

2022 ◽

Vol ahead-of-print (ahead-of-print) ◽

Author(s):

Marwa H. El-Azma ◽

Nadia M. El-Beih ◽

Karima A. El-Shamy ◽

Khaled M.M. Koriem ◽

Mahitab I. Elkassaby ◽

...

Keyword(s):

Oxidative Stress ◽

Cerebral Cortex ◽

Seed Oil ◽

Chronic Mild Stress ◽

Mild Stress ◽

Pumpkin Seed ◽

Content Type ◽

Tnf Α ◽

Pumpkin Seed Oil ◽

Cage Test

Purpose This study aims to investigate the potential of pumpkin seed oil (PSO) and zinc to attenuate oxidative stress and neuroinflammation caused by chronic mild stress (CMS) in the cerebral cortex of male rats. Design/methodology/approach The rats were submitted to stress for six weeks and then the behavior of the rats was tested by forced swimming test (FST) and novel cage test. The treated groups were given venlafaxine (20 mg/kg), pumpkin seed oil (40 mg/kg) and zinc (4 mg/kg). The cortex homogenate was used for the detection of the oxidative stress parameters, the concentration of neurotransmitters, tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β), Na+/K+-ATPase activity, and the expression of histamine N-methyltransferase (Hnmt) and tyrosine hydroxylase (Th). Findings CMS causes a significant increase in immobility time in the FST and a significant decrease in the number of rearing in the novel cage test. CMS group showed a significant increase in alanine aminotransferase (ALT) activity, levels of cortisol, TNF-α, IL-1β, nitric oxide and malondialdehyde. CMS caused a significant decrease in the concentrations of serotonin, GABA, norepinephrine, and the activities of glutathione peroxidase, catalase, superoxide dismutase and Na+/K+-ATPase. CMS caused a marked reduction in the expression of Hnmt and Th in the cortex. PSO and zinc attenuated the Na+/K+-ATPase activity, oxidative parameters and neuroinflammation induced by the CMS, and this was reflected by the elevation of the concentration of neurotransmitters and reduction of cortisol and ALT, in addition to the behavior normalization. PSO and zinc attenuated the CMS by improving the antioxidant milieu and anti-inflammatory status of the cerebral cortex. Originality/value There are no studies on the effect of pumpkin seed oil on depression

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Pharmacological study on the possible involvement of a Nrf2 -Heme oxygenase pathways in anti-cataract activity of fisetin

International Journal of Research in Phytochemistry and Pharmacology ◽

10.26452/ijrpp.v10i1.1156 ◽

2020 ◽

Vol 10 (1) ◽

pp. 14-19

Author(s):

Krishna Reddy BV ◽

Avinash Kumar Reddy G ◽

Sujitha V ◽

Manasa A

Keyword(s):

Oxidative Stress ◽

Heme Oxygenase ◽

Pharmacological Study ◽

World Population ◽

Central Feature ◽

Beneficial Effects ◽

Radical Production ◽

Anti Oxidant ◽

Diabetic Population ◽

Oxidant Status

DM otherwise diabetes is now a days an epidemic with the percentage of patient population rising to almost 10% of the world population. Out of all the DM complications, cataract leads the way contributing to disabilities to about 60% of diabetic population. But the pathogenesis of DM cataract is still a half-understood area of medicine there by posing a problem in the therapy. The data that we have till now gives us enough evidence to advocate the oxidative stress has a major role for the pathogenesis of DM complications like DMnephropathy, DMneuropathy, and cardiac hypertrophy, which suggests the oxidative stress is a central feature of diabetes. In the current research, the pharmacological evaluation of Fisetin for its DM based anti-cataract property was performed. This research concentrates to estimate the possible involvement of Nrf-2 / heme oxygenase (HO)-pathway in the observed therapeutic effect, if any. The data obtained in this study also indicate that the observed beneficial effects mainly due to activation of Nrf2/HO-1 pathway. These effects probably result in increased tissue anti-oxidant status as well as decreased free radical production, which ultimately responsible for the observed beneficial effects of Fisetin against hyperglycemia-induced cataract.

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The possible protective role of pumpkin seed oil in ameliorating tongue mucosal damage induced by orlistat in adult male albino rats: A light and scanning electron microscopic study

Egyptian Journal of Histology ◽

10.21608/ejh.2020.25027.1258 ◽

2020 ◽

Vol 0 (0) ◽

pp. 0-0

Author(s):

Amira Kassab ◽

Khalid Ahmed Moustafa ◽

Amal Abd-El-Hafez

Keyword(s):

Seed Oil ◽

Mucosal Damage ◽

Protective Role ◽

Albino Rats ◽

Electron Microscopic ◽

Pumpkin Seed ◽

Scanning Electron Microscopic Study ◽

Scanning Electron Microscopic ◽

Pumpkin Seed Oil

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Phenolics dynamics and infrared fingerprints during the storage of pumpkin seed oil and thereof oleogel

10.21748/am20.231 ◽

2020 ◽

Author(s):

Vlad Muresan

Keyword(s):

Seed Oil ◽

Pumpkin Seed ◽

Pumpkin Seed Oil

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Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients

BioMed Research International ◽

10.1155/2014/843157 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Tatjana Ruskovska ◽

Eugene H. J. M. Jansen ◽

Risto Antarorov

Keyword(s):

Oxidative Stress ◽

Statistical Significance ◽

Hemodialysis Patients ◽

Chronic Hemodialysis ◽

Total Serum ◽

Antioxidant Assays ◽

Hemodialysis Session ◽

Anti Oxidant ◽

Oxidant Status ◽

Healthy Persons

Background. Various biomarkers and assays have been used for assessment of (anti)oxidant status in hemodialysis patients, including those intended for measurement of serum total (anti)oxidants, most often as a part of panel biomarkers.Methods. Serum (anti)oxidant status was measured in 32 chronically hemodialyzed patients and in 47 healthy persons, using two oxidations and three antioxidant assays.Results. The patients before the hemodialysis session have had higher values of total oxidants in comparison to the healthy persons, with a further increase during the hemodialysis. These findings were confirmed with both oxidation assays, but they differ in the percentage of increase and the statistical significance. All three antioxidant assays showed significantly higher values of the total serum antioxidants in the patients before the hemodialysis session in comparison to the healthy persons, and their significant decrease during the hemodialysis. However, the assays differ in the percentage of decrease, its statistical significance, and the correlations with uric acid.Conclusion. The variability of results of total (anti)oxidants which are obtained using different assays should be taken into account when interpreting data from clinical studies of oxidative stress, especially in complex pathologies such as chronic hemodialysis.

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