scholarly journals Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Tatjana Ruskovska ◽  
Eugene H. J. M. Jansen ◽  
Risto Antarorov
Keyword(s):  
Oxidative Stress ◽  
Statistical Significance ◽  
Hemodialysis Patients ◽  
Chronic Hemodialysis ◽  
Total Serum ◽  
Antioxidant Assays ◽  
Hemodialysis Session ◽  
Anti Oxidant ◽  
Oxidant Status ◽  
Healthy Persons

Background. Various biomarkers and assays have been used for assessment of (anti)oxidant status in hemodialysis patients, including those intended for measurement of serum total (anti)oxidants, most often as a part of panel biomarkers.Methods. Serum (anti)oxidant status was measured in 32 chronically hemodialyzed patients and in 47 healthy persons, using two oxidations and three antioxidant assays.Results. The patients before the hemodialysis session have had higher values of total oxidants in comparison to the healthy persons, with a further increase during the hemodialysis. These findings were confirmed with both oxidation assays, but they differ in the percentage of increase and the statistical significance. All three antioxidant assays showed significantly higher values of the total serum antioxidants in the patients before the hemodialysis session in comparison to the healthy persons, and their significant decrease during the hemodialysis. However, the assays differ in the percentage of decrease, its statistical significance, and the correlations with uric acid.Conclusion. The variability of results of total (anti)oxidants which are obtained using different assays should be taken into account when interpreting data from clinical studies of oxidative stress, especially in complex pathologies such as chronic hemodialysis.

Pharmacological study on the possible involvement of a Nrf2 -Heme oxygenase pathways in anti-cataract activity of fisetin

2020 ◽  
Vol 10 (1) ◽  
pp. 14-19
Author(s):  
Krishna Reddy BV ◽  
Avinash Kumar Reddy G ◽  
Sujitha V ◽  
Manasa A
Keyword(s):  
Oxidative Stress ◽  
Heme Oxygenase ◽  
Pharmacological Study ◽  
World Population ◽  
Central Feature ◽  
Beneficial Effects ◽  
Radical Production ◽  
Anti Oxidant ◽  
Diabetic Population ◽  
Oxidant Status

DM otherwise diabetes is now a days an epidemic with the percentage of patient population rising to almost 10% of the world population. Out of all the DM complications, cataract leads the way contributing to disabilities to about 60% of diabetic population. But the pathogenesis of DM cataract is still a half-understood area of medicine there by posing a problem in the therapy. The data that we have till now gives us enough evidence to advocate the oxidative stress has a major role for the pathogenesis of DM complications like DMnephropathy, DMneuropathy, and cardiac hypertrophy, which suggests the oxidative stress is a central feature of diabetes. In the current research, the pharmacological evaluation of Fisetin for its DM based anti-cataract property was performed. This research concentrates to estimate the possible involvement of Nrf-2 / heme oxygenase (HO)-pathway in the observed therapeutic effect, if any. The data obtained in this study also indicate that the observed beneficial effects mainly due to activation of Nrf2/HO-1 pathway. These effects probably result in increased tissue anti-oxidant status as well as decreased free radical production, which ultimately responsible for the observed beneficial effects of Fisetin against hyperglycemia-induced cataract.

Higher Serum Bisphenol A Levels in Diabetic Hemodialysis Patients

2016 ◽  
Vol 42 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Faruk Turgut ◽  
Sana Sungur ◽  
Ramazan Okur ◽  
Mustafa Yaprak ◽  
Muge Ozsan ◽  
...  
Keyword(s):  
Bisphenol A ◽  
High Performance ◽  
Patient Characteristics ◽  
Hemodialysis Patients ◽  
Chronic Hemodialysis ◽  
Dialysis Session ◽  
Laboratory Parameters ◽  
Hemodialysis Session ◽  
Before And After ◽  
Patients With Diabetes

Background: Bisphenol A (BPA) has been implicated as an ‘endocrine disruptor'. We aimed at exploring the association between serum BPA levels and patient characteristics, particularly the presence of diabetes mellitus, and laboratory parameters in hemodialysis patients. Methods: This study included 47 chronic hemodialysis patients. Patient characteristics were recorded. Blood was drawn before and after hemodialysis session. Serum BPA levels were measured by the high-performance-liquid-chromatography and laboratory parameters were measured by using standard methods. Results: In hemodialysis patients, postdialysis serum BPA levels were significantly higher than predialysis after a single hemodialysis session (5.57 ± 1.2 vs. 4.06 ± 0.73, p < 0.0001). Predialysis serum BPA levels were significantly higher in patients with diabetes than non-diabetics (4.4 ± 0.6 vs. 3.9 ± 0.7, p = 0.025). No association was found between serum BPA levels and patient characteristics, and particularly laboratory parameters. Conclusion: Serum BPA levels were rising significantly after a single dialysis session. Diabetic hemodialysis patients had higher predialysis serum BPA levels.

Oxidative stress and other risk factors for white matter lesions in chronic hemodialysis patients

2013 ◽  
Vol 80 (09) ◽  
pp. 187-197 ◽  
Author(s):  
Mònica Muñoz-Cortés ◽  
Carme Cabré ◽  
Diasol Villa ◽  
Joan Pere Vives ◽  
Mercedes Arruche ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
White Matter ◽  
White Matter Lesions ◽  
Hemodialysis Patients ◽  
Chronic Hemodialysis

Selected root plant supplementation reduces indices of exercise-induced muscle damage: A systematic review and meta-analysis

2020 ◽  
pp. 1-21
Author(s):  
Kenji Doma ◽  
Baily Devantier-Thomas ◽  
Daniel Gahreman ◽  
Jonathan Connor
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Muscle Damage ◽  
Performance Measures ◽  
Muscle Soreness ◽  
Meta Analysis ◽  
Muscular Performance ◽  
Anti Oxidant ◽  
Exercise Induced ◽  
Oxidant Status

Abstract. This systematic review and meta-analysis examined the effects of selected root plants (curcumin, ginseng, ginger and garlic) on markers of muscle damage and muscular performance measures following muscle-damaging protocols. We included 25 studies (parallel and crossover design) with 353 participants and used the PEDro scale to appraise each study. Forest plots were generated to report on standardised mean differences (SMD) and p-values at 24 and 48 hours following the muscle-damaging protocols. The meta-analysis showed that the supplemental (SUPP) condition showed significantly lower levels of indirect muscle damage markers (creatine kinase, lactate dehydrogenase and myoglobin) and muscle soreness at 24 hours and 48 hours (p < 0.01) than the placebo (PLA) condition. The inflammatory markers were significantly lower for the SUPP condition than the PLA condition at 24 hours (p = 0.02), although no differences were identified at 48 hours (p = 0.40). There were no significant differences in muscular performance measures between the SUPP and PLA conditions at 24 hours and 48 hours (p > 0.05) post-exercise. According to our qualitative data, a number of studies reported a reduction in oxidative stress (e.g., malondialdehyde, superoxide dismutase) with a concomitant upregulation of anti-oxidant status, although other studies showed no effects. Accordingly, selected root plants minimised the level of several biomarkers of muscle damage, inflammation and muscle soreness during periods of exercise-induced muscle damage. However, the benefits of these supplements in ameliorating oxidative stress, increasing anti-oxidant status and accelerating recovery of muscular performance appears equivocal, warranting further research in these outcome measures.

scholarly journals Evaluation of anti-oxidant status in-vitro and in-vivo in hydro-alcoholic extract of Eugenia caryophyllus

2016 ◽  
Vol 4 (1) ◽  
pp. 19
Author(s):  
Roma Ghai ◽  
Kandasamy Nagarajan ◽  
Jitendra Singh ◽  
Shiwam Swarup ◽  
Minu Keshari
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Flavonoid Content ◽  
Anti Oxidant ◽  
Oxidant Status

<p>Free radicals mediated oxidative stress is the major risk factor for many chronic diseases like atherosclerosis, diabetes mellitus, arthritis, cancer, ageing and neurodegenerative diseases. Therapy with anti-oxidants is gradually gaining lot of importance for treatment of such diseases. Hydro-alcoholic extract of <em>Eugenia caryophyllus</em> was studied for its <em>in-vivo</em> antioxidant activity using two different animal models viz. Triton induced hyperlipidemia and High fat diet induced hyperlipidemia. Total phenolic content and total flavonoid content, DPPH assay was also carried out for <em>in vitro</em> anti-oxidant efficacy. Total protein, lipid peroxidation (MDA), reduced glutathione, superoxide dismutase and catalase were evaluated in the liver tissue in Triton induced hyperlipidemia and diet induced hyperlipidemia models. The study findings indicated significant <em>in-vivo</em> and <em>in-vitro</em> antioxidant property that may be related to the amount of polyphenols and flavonoids present in the extract. These results clearly indicate that <em>Eugenia caryophyllus</em> is effective against free radical mediated oxidative stress.</p>

scholarly journals Biochemical Evaluation of the Effects of Quercetin On Experimental Acute Methanol Intoxication in Rats

2020 ◽  
pp. 195-201
Author(s):  
Hasan Hüseyin Kozak ◽  
İbrahim Kılınç ◽  
Alpaslan Özkürkçüler
Keyword(s):  
Oxidative Stress ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Maximum Serum ◽  
Methanol Intoxication ◽  
Oxidative Stress Index ◽  
Group A ◽  
Anti Oxidant ◽  
Level Elevation ◽  
Oxidant Status

Previous studies have shown the role of oxidative stress in methanol toxicity. Also, studies have shown antioxidant, anti-inflammatory effects of quercetin (Q). This study evaluates the effect of quercetin (Q) administration on total oxidant status (TOS), total anti-oxidant status (TAS), oxidative stress index (OSI) and paraoxanase 1 (PON1) levels in rats with experimentally-induced methanol (MeOH) intoxication. Six groups were constituted as control, methotrexate (Mtx), Mtx+MeOH, Mtx+MeOH+ethanol (EtOH), Mtx+MeOH+Q1, Mtx+MeOH+Q2. All rats except controls were injected Mtx (0.3 mg/kg daily) intra-peritoneally (IP) for 7 days. On the 8th day of the test, 3 g/kg MeOH was injected IP in MeOH, EtOH and Q groups. Four hours after MeOH administration, 0.5 g/kg EtOH was injected IP in EtOH group and 50 mg/kg Q was administered IP in Q1 and Q2 groups. In addition, a total of 5 doses of 50 mg/kg Q was injected IP 24, 48, 72 and 96 hours after the first dose in Q2 group. Saline solution was given IP in the other groups. Rats were sacrificed with anesthesia 8 hours after the administrations. Blood samples were obtained for evaluating total oxidant status (TOS), total anti-oxidant status (TAS), oxidative stress index (OSI) and paraoxanase 1 (PON1) levels. The highest TOS level was found in MeOH+MTx group. A significant reduction was detected in serum TOS levels in MeOH+Mtx+EtOH, MeOH+Mtx+Q1 and MeOH+Mtx+Q2 groups. The lowest serum TAS level was detected in MeOH+Mtx group. Maximum TAS level elevation was found in MeOH+Mtx+Q2, MeOH+Mtx+Q1 and MeOH+Mtx+EtOH groups. The highest OSI ratio was found in MeOH+Mtx group. A reduction was detected in OSI ratios in MeOH+Mtx+EtOH, MeOH+Mtx+Q1 and MeOH+Mtx+Q2 groups as compared to MeOH+Mtx group. The lowest serum PON1 level was found in MeOH+Mtx group. Maximum serum PON1 level elevation was found in MeOH+Mtx+Q2 group. The results indicating that quercetin administration could be effective on both acute and subacute processes of methanol intoxication were tried to be revealed through serum TOS, TAS, OSI and PON1 levels. These results show that quercetin could be used as an alternative treatment option in methanol intoxication.

scholarly journals MO870HYPOCHLOREMIA IS ASSOCIATED WITH A GREATER INCIDENCE OF PNEUMONIA IN CHRONIC HEMODIALYSIS PATIENTS WITH COVID-19: A CENTER'S EXPERIENCE

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Francisco Valga ◽  
Tania Monzon ◽  
Nicanor Vega-Diaz ◽  
Sergio Ruiz-Santana ◽  
Rassoul Diallo-Saavedra ◽  
...  
Keyword(s):  
Hospital Admission ◽  
Statistical Significance ◽  
Chloride Concentration ◽  
Length Of Hospital Stay ◽  
Hemodialysis Patients ◽  
Chronic Hemodialysis ◽  
Lower Serum ◽  
Cardiovascular Comorbidity ◽  
Serum Chloride ◽  
Pcr Test

Abstract Background and Aims Recently, serum chloride has gained greater importance in the assessment of patients with heart failure and sepsis. Hypochloremia has been associated with higher mortality. On the other hand, COVID-19 pandemic continues to be, to date, a threat to public health. Patients with cardiovascular comorbidity or chronic kidney disease are particularly vulnerable. There are some studies that show a trend towards a lower serum chloride concentration in patients with a positive PCR test for SARS-CoV-2. Therefore, the objective of our study was to determine if there is a relationship between serum chloride levels at the time of diagnosis and a greater tendency to develop COVID-19 pneumonia in chronic hemodialysis patients. Method Retrospective cohort study. We analyzed the serum chloride, C-reactive protein (CRP), procalcitonin, neutrophil-lymphocyte (NLR) and platelet-lymphocyte (PLR) ratios of 11 chronic hemodialysis patients with a positive SARS-CoV-2 TMA PCR test during the second wave of the pandemic in our hospital (August-December 2020). We collected the length of hospital stay, the diagnosis of pneumonia (yes/no) and the final state of the infection (cure or death). The patients were divided into two groups taking the median serum chloride as the cut-off point (1: &lt;97 mEq / L and 2:&gt; 97mEq / L) Results The mean age was 57 ± 13 years and 36.36% (N = 4) were women. All patients required hospital admission and mean hospitalization time was 19 ± 13 days. 3 patients (27.3%) died. The medians of the parameters were the following: serum chloride 97 mEq / L (IQR 94-99); CRP 29.04 mg / L (IQR 8.53-76.13); NLR 4.13 (IQR 2.67-8.48) and PLR 244.06 (IQR 208.08-320). 81.8% (N = 9) had COVID-19 pneumonia. Group 1 patients (Chloride &lt;97 mEL / L) had a higher incidence of pneumonia (p = 0.049) (Figure 1) and a greater tendency to be admitted to the Intensive Care Unit (ICU) (p = 0.029). Despite not reaching statistical significance, there was also a higher mortality in patients with lower chloride levels. Conclusion Chronic hemodialysis patients with SARS-CoV-2 infection and lower serum chloride levels at hospital admission were more likely to develop pneumonia.

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