Preparation and Characterization of Cellulose and Microcrystalline Cellulose from Sugarcane Bagasse and Assessment of the Microcrystalline Cellulose as a Directly Compressible Excipient

2021 ◽  
Vol 36 (2) ◽  
pp. 81-96
Author(s):  
Degefu Natanim ◽  
Gabriel Tesfaye ◽  
Belete Anteneh ◽  
Gebre-Mariam Tsige
Keyword(s):  
Thermal Stability ◽  
Particle Size ◽  
Sugarcane Bagasse ◽  
Microcrystalline Cellulose ◽  
Dry Weight ◽  
Flow Property ◽  
Degree Of Polymerization ◽  
Degree Of Crystallinity ◽  
Scanning Electron Micrographs ◽  
Alternative Source

Cellulose, the most abundant biomass material in nature finds wide applications in the pharmaceutical industry. Sugarcane bagasse (SCB)  is one of the main agricultural lignocellulose byproducts. The objective of this study was to prepare and characterize native and  microcrystalline cellulose (MCC) from SCB and evaluate the MCC as a directly compressible pharmaceutical excipient. Cellulose was  extracted from SCB by chlorine-free methods with or without dewaxing. MCC was prepared from the cellulose by hydrolysis using  hydrochloric acid, and subsequently oven-dried (OD) or spray-dried (SD). The as-obtained cellulose and MCC were characterized in terms of yield, degree of polymerization (DP), chemical functionality, crystallinity, morphology and thermal stability. The chemical composition,  particle size, densities and direct compressibility of MCCs were also determined. Cellulose yields on a dry weight basis were found to be  42.8 ± 1.10% and 43.5 ± 0.5% from non-dewaxed and dewaxed SCB, respectively. Dewaxed SCB cellulose (DSCBC) provided higher yield of MCC (DSCB-MCCOD, 83 ± 0.74%) than non-dewaxed SCB cellulose (SCBC) (SCB-MCCOD; 78 ± 1.07%). The DP of SCBC and DSCBC were  580.56 and 592.75, respectively, while the DP of MCC ranged from 230.10 - 251.40. The FTIR spectra of both cellulose and MCC were similar with that of Avicel PH-101. The degree of crystallinity of the dewaxed cellulose (77.34%) and MCCOD (79.56%) and MCC-SD (81.87%) were  higher than non-dewaxed cellulose (74.50%) and MCC-OD (78.11%) and MCC-SD (79.62%). Scanning electron micrographs (SEM) showed a  fibrous structure for DSCBC and rod-shaped for DSCB-MCC. Thermogravimetric analysis (TGA) revealed dewaxed products exhibited better thermal stability than non-dewaxed products. All MCC samples exhibited monomodal normal particle size distributions. The Hausner ratio and Carr’s index of DSCB-MCCOD and Avicel PH-101 were not significantly different (p < 0.05) indicating similar flow property and  compressibility of the materials, respectively. Also, plain tablets prepared from SCB-MCC showed reasonably high crushing strengths (MCC-SD > MCC-OD), although tablets of Avicel PH-101 showed the highest crushing strengths. Thus, SCB could be an alternative source of cellulose and MCC for pharmaceutical applications.

scholarly journals COMPARATIVE STUDIES OF PHYSIC-CHEMICAL PROPERTIES AND STRUCTURE OF COTTON CELLULOSE AND ITS MODIFIED FORMS

2019 ◽  
pp. 5-13
Author(s):  
Abdumutalib Abdupattaevich Atahanov ◽  
Burkhon Mamadiyorov ◽  
Makhliyo Kuzieva ◽  
Svetlana Mikhaylovna Yugay ◽  
Sirozh Shahobutdinov ◽  
...  
Keyword(s):  
Particle Size ◽  
Microcrystalline Cellulose ◽  
Comparative Studies ◽  
Size Structure ◽  
Periodate Oxidation ◽  
Degree Of Polymerization ◽  
Cotton Cellulose ◽  
Degree Of Crystallinity ◽  
Large Temperature ◽  
Colloidal Systems

Comparative studies of the physicochemical properties and structures of cotton cellulose, microcrystalline cellulose, and nanocellulose were carried out using IR, NMR spectroscopy, X-ray diffraction, thermal analysis in order to identify the dependence "particle size - structure - properties". It was revealed that in the series “cotton cellulose – microcrystalline cellulose – nanocellulose” the degree of polymerization decreases (1200, 230, 110 respectively), the degree of crystallinity increases (66%, 72%, 83% respectively). The IR spectra of microcrystalline cellulose and nanocellulose are characterized by sharp peaks (in the range 1000–1500 cm–1) compared with cotton cellulose. The amount of bound water in gels of microcrystalline cellulose and nanocellulose increases with decreasing particle size, the degree of stability of colloidal systems increases with the transition from microcrystalline cellulose to nanocellulose. Nanocellulose and microcrystalline cellulose have relatively smaller mass loss and relatively large temperature ranges of intensive decomposition and their thermal stability is higher than cotton cellulose. It was found that the periodate oxidation rate of nanocellulose is higher than that of microcrystalline cellulose and cotton cellulose. It was established that microcrystalline cellulose is quantitatively susceptible to periodate oxidation in 7–8 hours, and nanocellulose in 0.5–1 hour.

scholarly journals Non-Acidic Method for Isolation of Microcrystalline Cellulose From Oil Palm Empty Fruit Bunch Fiber

2019 ◽  
Vol 7 (4.14) ◽  
pp. 339
Author(s):  
N. Samat ◽  
N. N. Ghazali ◽  
Z. Ahmad ◽  
F. Ali
Keyword(s):  
Thermal Stability ◽  
Microcrystalline Cellulose ◽  
Oil Palm ◽  
Milling Time ◽  
Crystallinity Index ◽  
Ammonium Persulfate ◽  
Milling Process ◽  
Degree Of Crystallinity ◽  
Empty Fruit Bunch ◽  
Oxidation Treatment

In this study, the microcrystalline cellulose (MCC) was extracted via a non-acidic method from the oil palm empty fruit bunch (EFB) cellulose. The extraction was conducted through Ammonium Persulfate (APS) oxidation treatment, which was followed by the ball milling process. The effects of varied temperature levels from APS oxidation treatment (60, 80 and 90oC) and different milling time (1, 4 hours) were investigated. APS oxidation treatment at 90oC was found to produce the most optimum results. The size of the MCC was less than 20μm and had demonstrated the highest degree of crystallinity index and thermal stability. The high crystallinity index is associated with the removal of non-cellulosic components as seen from FTIR analysis, where a decrease was observed in the characteristic peak intensity of 1735 and 1510 cm-1. The milling time had also affected the formation of MCC. Although a relatively longer milling time had produced smaller MCC with narrow size distribution, it had, however, given rise to a slight adverse effect on the crystallinity index and thermal stability.              

scholarly journals Preparation and characterization of nanocellulose from sugarcane bagasse

2020 ◽  
Vol 10 (3) ◽  
pp. 5675-5678
Keyword(s):  
Thermal Stability ◽  
Particle Size ◽  
Acid Hydrolysis ◽  
Sugarcane Bagasse ◽  
Xrd Analysis ◽  
X Ray Diffraction ◽  
Hydrolysis Time ◽  
Chemical Treatments ◽  
Thermal Stability Of

Nanocellulose (NC) was extracted from sugarcane bagasse (SCB) by acid hydrolysis. Alkalization and bleaching were used to treat SCB before the acid hydrolysis. The hydrolysis was carried out at 45 and 60℃ for 90 and 180 min. Chemical structure, crystallinity and thermal stability of the materials were studied using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis and thermogravimetric analysis (TGA), respectively. Morphology and particle size of nanocellulose were also studied using field emission scanning electron microscope (FE-SEM). FTIR results confirmed that lignin and hemicellulose were eliminated after alkali and bleaching treatments. These chemical treatments resulted in an improvement in the crystallinity and thermal stability of SCB. Sphere shape nanocellulose particles were observed by FE-SEM. With increasing hydrolysis time and temperature, the crystallinity of nanocellulose was increased but particle size and thermal stability were decreased.

scholarly journals Isolation and characterization of regenerated cellulose films using microcrystalline cellulose from oil palm empty fruit bunch with an ionic liquid

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8268-8290
Author(s):  
P. R. D. Weerasooriya ◽  
H. P. S. Abdul Khalil ◽  
Noor Haida Mohd Kaus ◽  
Md. Sohrab Hossain ◽  
Salim Hiziroglu ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Ionic Liquid ◽  
Water Vapor ◽  
Microcrystalline Cellulose ◽  
Oil Palm ◽  
Water Vapor Permeability ◽  
Degree Of Crystallinity ◽  
Vapor Permeability ◽  
Regenerated Cellulose ◽  
Empty Fruit Bunch

There is increasing interest in regenerated cellulose (RC) films for advanced manufacturing applications using natural polymers and renewable materials. In this study, RC films were isolated via solution casting process using microcrystalline cellulose (MCC) and the ionic liquid 1-butyl-3-methylimidazolium chloride ([BMIM]Cl). Initially, MCC was synthesized from oil palm empty fruit bunch using total chlorine-free (TCF) pulping and acid hydrolysis. Effects of MCC on the structures and physicochemical properties of the isolated RC films were determined for 4 wt%, 6.5 wt%, and 9 wt% of MCC at 80 °C. Several analytical methods were employed to evaluate degree of crystallinity, chemical stability, mechanical properties, morphology, opacity, water vapor permeability, and thermal stability of the RC films. The results showed that the addition of 6.5 wt% of MCC yielded the greatest tensile strength. Compared with the RC films with 6.5 wt% of MCC, thermal stability and water vapor permeability slightly increased when the MCC content was 9 wt%. According to the analytic hierarchy process (AHP), 6.5 wt% of MCC was the optimum MCC concentration to mix with [BMIM]Cl to manufacture RC films for packaging applications, while 9 wt% of MCC was ideal for photocatalytic and electrically conductive thin film applications.

scholarly journals Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar
Keyword(s):  
Cell Wall ◽  
Sugarcane Bagasse ◽  
Structural Changes ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Gravimetric Analysis ◽  
Chemical Pretreatment ◽  
Cell Wall Degrading Enzymes ◽  
First Time ◽  
Soluble Components

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

scholarly journals Comparison of the Bone Harvesting Capacity of an Intraoral Bone Harvesting Device and Three Different Implant Drills

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Hyun-Chang Lim ◽  
Kyung-In Ha ◽  
Ji-Youn Hong ◽  
Ji-Young Han ◽  
Seung-Il Shin ◽  
...  
Keyword(s):  
Particle Size ◽  
Dry Weight ◽  
Volumetric Measurement ◽  
Small Particles ◽  
Low Speed ◽  
Bone Particle ◽  
Bone Harvesting ◽  
Bone Particles

The aim of the present study was to compare bone-collecting capacity of bone harvesting device and minimally irrigated low-speed drilling using three implant systems. One bone harvesting device and three commercially available drill systems were compared using the osteotomies on bovine rib bones. The amount of the collected bone particle and particle size (<500 μm: small, 500–1000 μm: medium, and >1000 μm: large) were measured. Total wet (1.535±0.232 mL) and dry volume (1.147±0.425 mL) of the bone particles from bone harvesting device were significantly greater than three drill systems (wet volume: 1.225±0.187–1.27±0.29 mL and dry volume: 0.688±0.163–0.74±0.311 mL) (P<0.05). In all groups, the amount of large sized particles in wet and dry state was the greatest compared to that of medium and small particles. The dry weight of the bone particles showed the same tendency to volumetric measurement. In conclusion, total bone particles and large sized particles (>1000 μm) were harvested significantly greater by bone harvesting device than minimally irrigated low-speed drilling. The composition of particle size in all harvesting methods was similar to each other.

scholarly journals Improvement of Aspergillus flavus saponin hydrolase thermal stability and productivity via immobilization on a novel carrier based on sugarcane bagasse

2018 ◽  
Vol 17 ◽  
pp. 55-62 ◽  
Author(s):  
Hala A. Amin ◽  
Francesco Secundo ◽  
Hassan Amer ◽  
Faten A. Mostafa ◽  
Wafaa A. Helmy
Keyword(s):  
Thermal Stability ◽  
Aspergillus Flavus ◽  
Sugarcane Bagasse

scholarly journals A two-jet arc plasma: matrix effects and ways to their suppression

2019 ◽  
Vol 85 (1II)) ◽  
pp. 139-144
Author(s):  
N. P. Zaksas ◽  
A. F. Veryaskin
Keyword(s):  
Thermal Stability ◽  
Particle Size ◽  
Biological Samples ◽  
Matrix Effects ◽  
Certified Reference Materials ◽  
Organic Matrix ◽  
Graphite Powder ◽  
Powdered Sample ◽  
Experimental Conditions ◽  
Emission Analysis

A two-jet plasma is used for direct atomic emission analysis of powdered samples. It is characterized by relatively weak matrix effects, which allows using unified calibration samples on the basis of graphite powder for analysis of the samples with inorganic, organic, and organomineral matrix. In the present paper the effects limiting the usage of the unified approach due to different thermal stability and evaporation efficiency of the samples are discussed. The understated concentrations of a set of elements (Al, Ba, Ca, La, Mg, Mn, Sr, Ti, and Y) were obtained in analysis of certified reference materials of geological samples. It was shown that determination of rare earth elements should be carried out in the region behind the jet confluence providing their complete evaporation. For other elements, registration of the spectra in this region improves the results to some extent but they do not achieve the certified values. To speed up evaporation of these elements, the experimental conditions were chosen for plasma chemical reactions which provide conversion of the matrix elements into more volatile compounds. Addition of ammonium hydrofluoride to powdered sample considerably increased the line intensities of Al and Ca strongly associated with the silicon matrix. Incomplete evaporation was observed in analysis of biological samples with particle size more than 100 μm. A decrease in consumption of carrier argon is quite enough for effective decomposition of the organic matrix in plasma; the value of gas consumption depends on thermal stability and particle size of the sample. Preliminary sample carbonization is another way to improve evaporation of biological samples.

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