scholarly journals Biochemical implication of administration ofmethanol extract of Ocimum gratissimum leaf on haematological profile of Wistar rats

2020 ◽  
Vol 26 (2) ◽  
pp. 93-98
Author(s):  
Dasofunjo Kayode ◽  
Obem O. Okwari ◽  
Ujong P. Ujong ◽  
Boniface U. Ati ◽  
Christopher O. Igwe
Keyword(s):  
Wistar Rats ◽  
Methanol Extract ◽  
Control Group ◽  
Haematological Profile ◽  
Male Wistar Rats ◽  
Group A ◽  
Group B ◽  
And Control ◽  
Group D ◽  
Ameliorative Measure

In the search for medicinal plants that will provide ameliorative measure to patients with anaemic disorders. The methanol extract of O. gratissimum leaf was administered to Wistar rats for its effect on haematological profile. Twenty-eight (28) male Wistar rats ranging from 180-200g was randomly picked and placed into plastic cages labeled A-D. Group A served as the control group while groups B-D was the test groups. The  animals in group A was administered with distilled water orally by gavage. Group B were administered 50mg/ kg body weight of methanol extract of O. gratissimum, group C were administered with 100mg/kg bodyweight while group D were administered with 200mg/body of methanol extract of O. gratissimum for 14 days. Blood was collected from all the test rats and control by cardiac puncture using disposable syringe and needle then dispensed into tubes containing EDTA. The extract displayed a significant increase (p<0.05) in RBCs, Hb, PCV and platelet counts. More so, the  extract produced no significant (p>0.05)difference in MCV, MCH, MCHC and RDW WBC counts when compared with the normal control .Therefore, it will be logical to conclude that the extract of O. gratissimum might be a panacea in the management of anaemic conditions when properly harnessed due to its erythropoietic, haematopoietic and thrombopoietic effect. Keywords: Anaemia, erythropoietic, haematopoietic, thrombopoietic and phytochemicals.

scholarly journals The effect of Dichlorvous (Sniper) Inhalation on the Histology of the Lungs of Adult Male Wistar Rats

2021 ◽  
pp. 58-67
Author(s):  
A. E. Anyabolu ◽  
D. N. Ezejindu ◽  
B. N. Obinwa
Keyword(s):  
Wistar Rats ◽  
The Body ◽  
Control Group ◽  
Male Wistar Rats ◽  
Water Feed ◽  
Group A ◽  
Cervical Dislocation ◽  
Group B ◽  
Histological Architecture ◽  
Group D

The organophosphate, dichlorvous (Otapia-pia) formulated in varying concentrations as insecticides is utilized by several individuals in most remote places of Nigeria due to its affordable value and accessibility. However, this present study is conducted to investigate the adverse effect of the exposure of this substance on the respiratory system (lungs) of male albino wistar rats. Twenty (20) albino wistar rats comprising of all males weighing between 150-230g were divided into four groups (A-D) of five animals each. Group A received only water, feed, and served as the control. Group B was exposed to dichlorvous inhalation 3hrs daily for a period of two weeks; Group C was exposed to dichlorvous inhalation 6hrs daily for a period of two weeks; while Group D was exposed to dichlorvous inhalation 10hrs daily for a period of two weeks. Twenty four hours after the last exposure, the animals were sacrificed by cervical dislocation and dissected. The lungs were weighed and fixed in 10% formal saline for histological studies. The body weight of the experimental groups decreased insignificantly when compared with the control group. The lungs weight increased significantly when compared to the control groups. Histological observation revealed a moderate to severe effects on the lungs with severe consolidated inflammatory exudates (discharge of fluids from pores), a moderate intra-alveoli hemorrhage, and a severe dilation of alveolar septa which is evident as emphysematous changes. This result revealed that the exposure to dichlorvous causes distortion of the normal histological architecture of the lungs. Thus, showing that dichlorvous inhalation influences the normal physiologic mechanisms of the lungs.

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

scholarly journals EFEK PEMBERIAN EKSTRAK KAYU MANIS (CINNAMOMUM BURMANNII) TERHADAP GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR YANG DIBERI ASPIRIN

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Janet Walangitan ◽  
Lily Loho ◽  
Meilany Durry
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Control Group ◽  
Therapeutic Effects ◽  
Cinnamon Extract ◽  
Group A ◽  
Cinnamomum Burmannii ◽  
Group B ◽  
Group D

Abstract: Cinnamon (Cinnamomum burmannii) is a traditional herbal plants which are often found in our daily life and has many benefits especially in health. This study were designed to know the effect of cinnamon on gastric mucosa given aspirin. This was an experimental research and used Wistar rats as the subject research.  The Wistar rats were randomly divided into 6 mice of control group and 9 mice of treatment group. Group A (K-) given pellets, group B (K+) given pellets and aspirin 150 mg/kgBB for 7 days, group C given pellets, aspirin 150 mg/kgBB and cinnamon extract 3 mg for 3 days, group D given pellets, aspirin 150 mg/kgBB for 7 days and given cinnamon extract 3 mg for 3 days, group E given pellets, aspirin 150 mg/kgBB for 7 days and given pellets only (without treatment) for 3 days. The results showed that aspirin cause gastric mucosa damage in group B (K+) compared with kelompok A (K-). Group C and D showed less inflammatory cells compared with group B (K+). Group E showed more inflammatory cells compared with group D. The study suggested that cinnamon extract has protective and therapeutic effects on gastric mucosa of Wistar rats. Keywords: cinnamon, gaster, aspirin.   Abstrak: Kayu manis (Cinnamomum burmannii) merupakan tanaman herbal tradisional yang banyak dijumpai dalam kehidupan sehari- hari dan memiliki banyak manfaat termasuk dalam bidang kesehatan. Penelitian ini bertujuan untuk mengetahui apakah ada efek pemberian kayu manis terhadap mukosa lambung yang diberi aspirin. Desain Penelitian yang digunakan adalah metode eksperimental dan subjek penelitian menggunakan tikus Wistar, yang terbagi atas 6 ekor kontrol dan 9 ekor perlakuan. Kelompok A (K-) diberikan pelet selama 7 hari, kelompok B (K+) diberikan pelet dan aspirin 150mg/kgBB selama 7 hari, kelompok C diberikan pelet, aspirin 150 mg/kgBB, ekstrak kayu manis 3 mg secara bersama- sama selama 7 hari, kelompok D diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian ekstrak kayu manis 3 mg selama 3 hari, kelompok E diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian pelet saja (tanpa perlakuan) selama 3 hari. Hasilnya menunjukkan aspirin menimbulkan kerusakan mukosa lambung pada kelompok B (K+) dibandingkan dengan kelompok A (K-). Kelompok C dan D menunjukkan sel-sel radang yang lebih sedikit dari kelompok B(kontrol +). Kelompok E menunjukkan sel- sel radang yang lebih banyak dibandingkan dengan kelompok D. Kesimpulan dari penelitian ini adalah kayu manis mempunyai efek protektif dan terapeutik terhadap mukosa lambung tikus Wistar. Kata kunci: kayu manis, lambung, aspirin.

scholarly journals GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR (Rattus norvegicus) YANG DIBERIKAN LENGKUAS (Alpinia galanga Willd) SETELAH DIINDUKSI OLEH ASAM MEFENAMAT

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Juita Pasaribu ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Wistar Rats ◽  
Acute Inflammation ◽  
Inflammatory Cells ◽  
Control Group ◽  
Treatment Groups ◽  
Acute Gastritis ◽  
Group A ◽  
Mucosal Lining ◽  
Group B ◽  
Group D

Abstrak: Gastritis akut merupakan proses inflamasi yang bersifat akut dan biasanya terjadi pada bagian permukaan mukosa lambung. Penyakit ini biasanya disebabkan karena banyak faktor salah satunya yaitu penggunaan asam mefenamat dalam dosis yang berlebihan. Pada penelitian yang telah dilakukan sebelumnya didapatkan bahwa lengkuas dapat mengurangi terjadinya radang akut. Tujuan penelitian ini adalah untuk mengetahui gambaran histopatologi lambung tikus wistar yang diberikan lengkuas setelah diinduksi dengan asam mefenamat. Penelitian ini menggunakan metode eksperimental dengan sampel 11 ekor tikus wistar yang dibagi dalam 4 kelompok perlakuan. Kelompok A tidak diberikan perlakuan. Kelompok B diberikan asam mefenamat 10 mg selama 7 hari. Kelompok C diberikan asam mefenamat 10 mg selama 7 hari kemudian diberikan perasan lengkuas selama 7 hari. Kelompok D diberikan asam mefenamat 10 mg kemudian tidak diberikan perlakuan selama 7 hari. Hasil penelitian ini menunjukkan bahwa gambaran mikroskopik lambung tikus wistar pada kelompok C terdapat sel-sel radang PMN yang lebih sedikit dibandingkan dengan kelompok B dan D. Simpulan: lengkuas dapat mengurangi sel-sel radang PMN pada mukosa lambung yang diberikan dengan asam mefenamat. Kata kunci: Lengkuas, Asam Mefenamat, Gastritis akut.   Abstract : acute gastritis is an acute inflammatory process normally occurring in the mucosal lining of the stomach. This disease is caused by a variety of factors, one of which is the use of mefenamat acid in large doses. Previous research has revealed that galangal can be used to reduce acute inflammation. The objective of this research was to reveal the histopathological pictures of stomach of wistar rats that treatment with galangal after induce by mefenamat acid. This study is an experimental, employing 11 wistar rats which were assigned for four treatment groups. Group A was the control group in which the rats received no treatment. Rats in group B were administered mefenamat acid 10 mg for 7 days. In group C, the rats were also administered mefenamat acid 10 mg for 7 days and then received galangal distillation for 7 days. In group D, rats were administered mefenamat acid 10 mg for 7 days and then for 7 days without receiving anymore mefenamat acid or galangal distillation. Results showed that microscopic pictures of stomach of wistar rats in group C were presented with PMN inflammatory cells fewer than rats in group B and D. Conclusion: galangal can reduce PMN inflammatory cells in mucosal lining of the stomach exposed to mefenamat acid. Key words: galangal, mefenamat acid,  acute gastritis.

scholarly journals Morphological and Biochemical Effects of Aqueous Extract of Moringa oleifera Leaf on Cobalt Chloride-Induced Cerebral Damage in Adult Wistar Rats

2021 ◽  
pp. 46-57
Author(s):  
A. J. Ajibade ◽  
A. E. Okeleye ◽  
I. A. Ogunmola
Keyword(s):  
Cerebral Cortex ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Cobalt Chloride ◽  
High Dose ◽  
Male Wistar Rats ◽  
Group A ◽  
Group B ◽  
Group D ◽  
The Brain

Cobalt induces hypoxia in the brain which leads to oxygen deprivation resulting in cognitive disturbance and decreased motor control. This study evaluated the effect of Moringa oleifera extract on the cobalt chloride-induced cerebral cortex of adult male wistar rats.  40 male wistar rats weighing (90 ± 120g) were used for the study and they were divided into 5 groups with each group containing 8 rats. Group A served as control which received distilled water, Group B was treated orally with Cobalt chloride at dose 45 mg/kg, Group C received cobalt chloride 45 mg/kg + low dose of Moringa oleifera extract 250 mg/kg for 52 days, Group D treated with cobalt chloride 45 mg/kg + high dose of Moringa oleifera extract 500 mg/kg and Group E treated with 500 mg/kg Moringa oleifera extract only and rats were sacrificed on the 53rd day by cervical dislocation. The brain of each rat was removed and weighed before half was fixed in formol calcium for histological analysis and the second half was used for oxidative stress parameters. The mean body weight of the wistar rats in group C and E increased significantly (P <0.05) while it decreased significantly (P <0.05) in group D. The biochemical analysis shows a significant increase (P<0.05) in the level of MDA in group B and a significant decrease (P<0.05) in group E. additionally, NO level shows a significant increase (P<0.05) in group B compared with control. SDH activity decreased significantly in group C, D, and E. Microscopic examination of the cerebral cortex in group B, C and D showed degenerative changes compared with normal histological features in A and E. The study concluded that cobalt chloride induced cerebral cortical damage while administration of Moringa oleifera extract attenuated the toxic effect of cobalt chloride in wistar rats.

scholarly journals Comparative study of efficacy of commercial anthelmintic response against gastrointestinal nematodes in goats of Jhenidah district, Bangladesh

2019 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Prodip Kumar Halder ◽  
Biplob Kumar Sarker ◽  
Md. Shah Alam ◽  
Jannatun Nime ◽  
Md. Tareq Mussa ◽  
...  
Keyword(s):  
Body Weight ◽  
Gastrointestinal Nematodes ◽  
Control Group ◽  
Economic Implication ◽  
Fecal Egg Count ◽  
Group A ◽  
Antiparasitic Drugs ◽  
Group B ◽  
And Control ◽  
Group D

Background: Parasitic disease constitutes 60-70% diseases affecting the animals and has serious economic implication in livestock entrepreneurship by direct and indirect production loss. Indiscriminate use of anthelmintic drugs has made the situation even more precarious. A similar problem was encountered in goat from Holidhani, Jhenidah, where goats with complain of intermittent diarrhea and loss of body condition was reported despite of routine deworming.Objective:  Determining the efficacy of conventional anthelmintics used and its comparison with some unexploited antiparasitic drugs for the same reason.Methods: Sixty-five goats were divided into five groups. Group A goats were kept as the control, Group B (I, II, III), group C (IV, V, VI), group D (VII, VIII, IX) and group E (X, XI, XII) goats were treated with levamisole, albendazole, fenbendazole and ivermectin respectively. All the treated and control goats were kept, housed for 21 days after the first treatment. Fecal samples were collected and counted on 1st, 7th, 14th and 21st day by using McMaster counting method.Results: Among the three doses of levamisole, albendazole, fenbendazole and ivermectin, the doses of 7.5, 7.5, 5.0 and 0.2 mg/kg body weight, body weight were found to be most effective against gastrointestinal nematodes in goats with a maximum reduction of fecal egg count to the extent of 95.38, 97.13, 98.08 & 99.16 percent respectively.Conclusion: The study revealed low efficacy of levamisole and hence ivermectin is a better drug than albendazole and fenbendazole to control gastrointestinal nematodes in goats. 

scholarly journals Comparative efficacy of imported fowl pox virus vaccine with locally produced one in backyard chicks

1970 ◽  
Vol 6 (1) ◽  
pp. 23-26 ◽  
Author(s):  
MR Islam ◽  
MSR Khan ◽  
MA Islam ◽  
MEH Kayesh ◽  
MR Karim ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Control Group ◽  
Post Vaccination ◽  
History Of ◽  
Group A ◽  
Group B ◽  
Protection And Control ◽  
And Control ◽  
Group D

A total of 80 experimentally reared backyard chicks with the history of non-vaccinated parents to FPV vaccine were used for the determination of persistence of maternally derived antibody in chicks and to compare the efficacy of Poxine® and DLS-FPV vaccine in protecting the chicks. Chicks were divided into five groups namely group A, B, C, D and E where each group contained 15 chicks except group A contained 20 chicks which was also used to determine the persistence of maternally derived antibody (MDA). Birds of group B and C were vaccinated with DLS-FPV at day 22 and 18 respectively through wing web puncture (WWP), whereas group D and E were vaccinated with Poxine® at day 36 and 18 respectively. Sera were randomly colleted from 10 chicks of each group at 7, 14 and 21 days of post vaccination for the determination of antibody titre using PHA test. Ten chickens from each group were challenged three weeks post vaccination with 106 EID50/0.1ml. "Take reaction" was recorded to assess the better immune response in different groups after vaccination. Birds of group B, C, D and E showed 100, 86.67, 93 and 86.67% "take reaction", respectively. The highest Mean PHA titre was found 217.60 ± 19.55 in the chicks of group B. After challenge, the birds of group B and D showed 100% protection, whereas birds of group C and E showed 93.33% protection and control group showed no protection. From the results of PHA test it may be concluded that both Poxine® and DLS-FPV vaccines are equally suitable and the chicks of nonvaccinated origin might be vaccinated with DLS-FPV at day 18 or 22 and 36 in case of Poxine® fowl pox virus vaccine. Key words: Efficacy, fowl pox virus vaccine, backyard chicks, age DOI = 10.3329/bjvm.v6i1.1334 Bangl. J. Vet. Med. (2008). 6 (1): 23-26

scholarly journals ASCORBIC ACID;

2017 ◽  
Vol 24 (10) ◽  
pp. 1495-1500
Author(s):  
Aftab Ahmed Shaikh ◽  
Umair Ali Soomro ◽  
Shomail Saeed Siddiqui ◽  
Kashif Rasheed Shaikh ◽  
Mumtaz Ali Qureshi
Keyword(s):  
Ascorbic Acid ◽  
Renal Injury ◽  
Wistar Rats ◽  
Renal Tissue ◽  
Control Group ◽  
Male Wistar Rats ◽  
Biochemical Testing ◽  
Group B ◽  
Animal House ◽  
And Control

Objectives: Investigating the Renoprotective effects of Ascorbic acid ingentamicin induced renal injury (GIRI) in male Wistar rats. Study design: Experimental study.Place and Duration: Animal house of Al-Tibri from September 2016 to December 2016.Methodology: 60 male Wistar rats were selected by non-probability purposive sampling,and were divided into three groups; Group A- control, Group B- Gentamicin induced renalinjury (GIRI) (70 mg/kg/bwt i.m) daily , and Group C- GIRI + AA (Ascorbic acid - 0.2 mg/kg/bwt) daily. Cardiac puncture was performed by a Disposable Syringe for blood sampling.Sera were used for biochemical testing. Renal tissue was stained with H & E for histologicalexamination. Statistix 8.1 software (USA) was used for data analysis at P≤ 0.05. Results:Blood urea and serum creatinine were elevated in the group B (GIRI) compared to GIRI+AAand the controls (P=0.0001). Serum superoxide dismutase, glutathione peroxidase, andcatalase were low in GIRI compared to GIRI+AA and control groups. Renal tissue in ascorbicacid treated rats showed improved tissue architecture. Conclusion: It is concluded that theAscorbic acid exerts Renoprotective effects in gentamicin induced renal injury in rat model.

scholarly journals GAMBARAN HISTOPATOLOGIK HATI TIKUS WISTAR YANG DIINDUKSI CCL4 DAN DIBERI AIR REBUSAN TANAMAN CAKAR AYAM (Selaginella doederleinii Hieron)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Anthonio B. Lengkong
Keyword(s):  
Wistar Rats ◽  
Water Extract ◽  
Control Group ◽  
Histological Features ◽  
Phytochemical Content ◽  
Histopathological Features ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Herbal Plant

Abstract: The plant Selaginella doederleinii is a herbal plant. Its phytochemical content including saponin, flavonoid and alkaloid can function as hepatoprotector. The objective of this study was to reveal the histopathological features of wistar rats induced with CCl4 and administered with water extract of the leaves of Selaginella doederleinii. This study was an experimental research employing 16 wistar rats. The rats were divided into 5 groups. Group A was the control group, whereas the other groups were treated with CCl4 for five days. The differences between these groups were as follows; after treatment with CCl4, rats in group B were terminated, rats in group C were fed with regular pellets, rats in group D were administered with water extract of the leaves of Selaginella doederleinii of 1.6 cc/day, and rats in group E were administered with water extract of  the leaves of Selaginella doederleinii of 3.2 cc/day. Results showed that the histological features of the liver of rats administered with water extract of  the leaves of Selaginella doederleinii for three days after treated with CCl4 exhibit numerous regeneration of liver cells. However, if the administration was prolonged for six days then fatty liver was observed. To conclude, Selaginella doederleinii can improve the histopathological features of the liver of rats induced with CCl4, however if the administration was prolonged then liver damage may occur. Keywords: Selaginella doederleinii, CCl4, histopathological features of the liver of wistar rats.   Abstrak: Tanaman cakar ayam merupakan sediaan herbal. Kandungan yang terdapat didalamnya seperti saponin, flavonoid dan alkaloid berfungsi sebagai hepatoprotektor. Penelitian ini bertujuan untuk melihat gambaran histopatologik hati tikus wistar yang diinduksi CCl4 dan diberi air rebusan tanaman cakar ayam. Penelitian ini merupakan penelitian eksperimental yang menggunakan 16 ekor tikus wistar sebagai sampel. Tikus wistar dibagi dalam 5 kelompok. Kelompok A merupakan kelompok kontrol, sedangkan kelompok lainnya diberi CCl4 selama 5 hari. Perbedaan antara keempat kelompok lainnya yaitu setelah pemberian CCl4 kelompok B langsung diterminasi, kelompok C diberi pakan standart, kelompok D diberi tanaman cakar ayam dosis 1,6 cc/hari, dan kelompok E diberi tanaman cakar ayam dosis 3,2 cc/hari. Kelompok C, D, dan E dilakukan 2 kali terminasi yaitu hari ke-3 dan ke-6. Setelah perlakuan, terminasi dilakukan untuk mengetahui gambaran makroskopis dan mikroskopis hati tikus wistar. Dari hasil pengamatan yang dilakukan, gambaran hati tikus yang diberi air rebusan tanaman cakar ayam selama 3 hari setelah induksi CCl4 menunjukkan banyak regenerasi sel hati, tetapi pada pemberian selama 6 hari gambaran yang terlihat adalah perlemakan hati. Pemberian air rebusan tanaman cakar ayam dapat memperbaiki gambaran histopatologik hati yang diinduksi CCl4dan dalam jangka waktu lebih panjang dapat menyebabkan kerusakan hati. Kata kunci: Tanaman cakar ayam, CCl4, gambaran histopatologik hati tikus wistar.

scholarly journals Evaluation Of Weight Changes On Wistar Rats Induced By Erythromycin And Aqueous Neem Leaves ExtractEvaluation Of Weight Changes On Wistar Rats Induced By Erythromycin And Aqueous Neem Leaves Extract

2018 ◽  
Vol 09 (01) ◽  
pp. 03-07
Author(s):  
Nausheen Adnan ◽  
Uzma Bukhari ◽  
Iffat Raza
Keyword(s):  
Wistar Rats ◽  
Relative Weight ◽  
Liver Weight ◽  
Control Group ◽  
Weight Changes ◽  
Neem Leaves ◽  
Group A ◽  
Group B ◽  
Hepatic Injuries ◽  
Group D

Objective: To evaluate weight changes on the liver of male (albino wistar) rats induced by erythromycin. To assess the outcome of aqueous Neem leaf extract on erythromycin induced hepatic injuries. Methodology: Eighty male albino wistar rats were taken randomly and were divided into 4 groups of 20 animals each. Group A as a control group. Group B rats were treated with erythromycin drug. Group C rats were treated with erythromycin along with Aqueous Neem leaves extract. Group D were given only Aqueous Neem leaves extract. Body weight of animals was recorded initially and also after experimentation. After 14 days animals were sacrificed, liver weight was also recorded. Results: The liver of erythromycin treated animals group B were little bigger in size and more reddish. However erythromycin plus Neem treated animals of group C livers were almost like liver of control group A and only Neem given group D animals, liver were exactly same appearance like control group A.Rise in absolute and relative weight of liver was because of drug toxicity. Conclusion: Its established from this recent study that Aqueous Neem leaves extract being a powerful antioxidant, decreased oxidative stress and hepatic injury caused by erythromycin.

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