Serological Investigation And Interpreting Serum Chemistry Profile Of Natural Infected Cattle By Foot And Mouth Disease

ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. For several years, vaccination of animals, which had proven to be successful for the eradication of the disease, has been forbidden in the United States and the European Community because of the difficulty of differentiating between vaccinated and infected animals. In this study, detailed investigations of the bovine humoral immune response against FMD virus (FMDV) were performed with the aim of identifying viral epitopes recognized specifically by sera derived from FMDV-infected animals. The use of overlapping 15-mer synthetic peptides, covering the whole open reading frame of FMDV strain O1K in a peptide enzyme-linked immunosorbent assay, allowed the identification of 12 FMDV strain O1K-specific linear B-cell epitopes. Six of these linear B-cell epitopes, located in the nonstructural proteins, were used in further assays to compare the reactivities of sera from vaccinated and infected cattle. Antibodies recognizing these peptides could be detected only in sera derived from infected cattle. In further experiments, the reactivity of the six peptides with sera from animals infected with different strains of FMDV was tested, and strain-independent infection-specific epitopes were identified. Thus, these results clearly demonstrate the ability of a simple peptide-based assay to discriminate between infected and conventionally FMD-vaccinated animals.

Download Full-text

Consistent change in the B–C loop of VP2 observed in foot-and-mouth disease virus from persistently infected cattle: Implications for association with persistence

Virus Research ◽

10.1016/j.virusres.2006.12.008 ◽

2007 ◽

Vol 125 (1) ◽

pp. 114-118 ◽

Cited By ~ 20

Author(s):

Jacquelyn Horsington ◽

Zhidong Zhang

Keyword(s):

Disease Virus ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Infected Cattle ◽

Persistently Infected ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Consistent Change

Download Full-text

Bovine Serum Panel for Evaluating Foot-and-Mouth Disease Virus Nonstructural Protein Antibody Tests

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870701900513 ◽

2007 ◽

Vol 19 (5) ◽

pp. 539-544 ◽

Cited By ~ 15

Author(s):

Satya Parida ◽

Lucy Fleming ◽

Debi Gibson ◽

Pip A. Hamblin ◽

Santina Grazioli ◽

...

Keyword(s):

Disease Virus ◽

Nonstructural Protein ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Infected Cattle ◽

Nonstructural Proteins ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Contact Exposure ◽

Antibody Tests

A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination. Virus replication and persistence had been characterized in all of the animals. The proportion of the sera scored positive by 5 tests for antibodies to the nonstructural proteins of FMDV varied, suggesting that the panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of this panel will help in assessment of new tests and quality control of existing methods.

Download Full-text

The application of new techniques to the improved detection of persistently infected cattle after vaccination and contact exposure to foot-and-mouth disease

Vaccine ◽

10.1016/j.vaccine.2005.06.012 ◽

2005 ◽

Vol 23 (44) ◽

pp. 5186-5195 ◽

Cited By ~ 33

Author(s):

S. Parida ◽

S.J. Cox ◽

S.M. Reid ◽

P. Hamblin ◽

P.V. Barnett ◽

...

Keyword(s):

Foot And Mouth Disease ◽

Mouth Disease ◽

Infected Cattle ◽

New Techniques ◽

Persistently Infected ◽

Foot And Mouth ◽

Contact Exposure

Download Full-text

Field serological investigation for peste des petits ruminants, foot-and-mouth disease, and bluetongue diseases in illegally introduced animals in Egypt

Veterinary World ◽

10.14202/vetworld.2020.1661-1666 ◽

2020 ◽

Vol 13 (8) ◽

pp. 1661-1666

Author(s):

Wafaa Abd El Wahab Hosny ◽

Eman Mohamed Baheeg ◽

Hala Abd El Raheem Aly ◽

Samia Said Abd El Nabi ◽

Nadia Maher Hanna

Keyword(s):

Early Detection ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Current Status ◽

Peste Des Petits Ruminants ◽

Serum Samples ◽

Serological Investigation ◽

Foot And Mouth ◽

And Control ◽

Study Laboratory

Aim: In this study, laboratory scoping on the viruses that cause peste des petits ruminants (PPR), bluetongue (BT), and foot-and-mouth disease (FMD) was performed to evaluate the current status of animals illegally introduced into Egypt. This study aims to help control these infectious illnesses and tries to prevent the introduction of other strains of these three viruses to Egypt, as these illnesses spread quickly if not controlled. Materials and Methods: In the year 2018, 62 serum samples were collected and serologically tested through competitive enzyme-linked immunosorbent assays (ELISA) kits to detect antibodies against PPR, BT, and FMD, which are three important transboundary infectious illnesses. Results: The results indicated that 60 out of 62 serum samples were positive for PPR antibodies (96.7%), 31 out of 62 were positive for FMD antibodies (50%), and 59 out of 62 serum samples were positive for BT antibodies (95%). Conclusion: This study revealed that PPR, FMD, and BT can be introduced into Egypt through the illegal introduction of sheep and goat from neighboring countries. Laboratory diagnostic abilities should be improved for the early detection and control of these illnesses.

Download Full-text

Heat-Intolerance Syndrome subsequent Foot-and-Mouth Disease in Cattle; ThiQar –Iraq

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v36i0e.433 ◽

2012 ◽

Vol 36 (0E) ◽

pp. 286-292

Author(s):

Adil M. Abbas

Keyword(s):

Cortisol Level ◽

Clinical Signs ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Infected Cattle ◽

Low Level ◽

Foot And Mouth ◽

Radio Immunoassay ◽

Low Levels ◽

High Level

The investigation on Heat-Intolerance Syndrome following foot and mouth disease (FMD) infection in cattle in ThiQar–Iraq, used 3ABC FMD ELISA kit, and Radio- immunoassay (RIA) to detect the cortisol level. From 105 there were 65(62%) infected cattle with FMD, which was high at 5- less 8 years old, while the cortrisol level showed three levels; normal (13-21 nmol⁄L), high and low levels were; 6(5.6%), 44(42%) and 55(52%) subsequently. More over the combined result of ELISA and RIA had divided cattle into six groups. First group of 40(38%) cattle infected with FMD and had low level of cortisol, this group containing 21(20%) with clinical signs of heat intolerance. Second group contain 22(21%) FMD infected cattle with high level of cortisol. Third group of 3(2.8%) FMD Infected cattle but normal cortisol level. Fourth group included 3(2.8%) not infected by FMD with normal cortisol level. Fifth group contain 22(21%) not infected with FMD but had high level of cortisol may related to stress. Sixth group consisted 15 (14%) cattle not infected with FMD and had low level of cortisol due to un known cause.Clinical signs of heat intolerance that showed in 21 head of cattle in the first group were: panting, overgrowth of hair coat, emaciation and seeking for shad. The diseased cow known locally as ”Mahrorah” meaning heat-intolerance.

Download Full-text