Brontaea debilis (Thomson, 1896) (Diptera: Cyclorrhapha: Muscidae) as host for Spalangia drosophilae Ashmead, 1885 (Hymenoptera: Pteromalidae: Spalanginae) in Brazil

The flies included in the infra-order Cyclorrhapha (Muscomorpha) have medical and veterinary importance, since they may produce myiasis and act in carrying pathogens to man and animals. The experiment was carried out at the Federal University of Goiás farm in Goiânia, Goiás, Brazil. Every fortnight, ten dishes black plastic containers containing bovine feces were exposed for fifteen days in the pastures. After this period, the feces were sent to the laboratory for pupae extraction. The experiments were carried out from April 2006 to December 2007. Ninety-two pupae of Brontaea debilis (Thomson, 1896) (Diptera: Muscidae), from bovine feces, from which 31 specimens of Spalangia drosophilae Ashmead, 1887 (Hymenoptera: Pteromalidae) were emerged. The percentage of parasitism was 33.7%. Spalangia drosophilae is cited in the literature as parasitoid of the pupae of Diptera including Chloropidae, Drosophilidae, Muscidae, Sarcophagidae and Sepsidae.

Parasitoids Collected from Animal Feces in Brazil

The purpose of the paper is to report the species of dipteran parasitoids in poultry feces on farms, buffalo, and cattle in the field in Brazil. The experiments were carried out from April 2006 to December 2007. The pupae were obtained by the flotation method. They were individually placed in gelatin capsules until the emergency of the adult flies or their parasitoids. The specie more frequent was S endius with 7.2%. Were obtained from bovine feces 628 pupae of dipterous in buffalo feces, 3,437 pupae were collected and from chicken feces 2,799 pupae, from which 78, 172 and 504 parasitoids emerged, respectively. The most frequent species in bovine, of buffalo and chicken feces were: Gnathopleura quadridentata Wharton (Hymenoptera: Braconidae) with 25.6%, Spalangia drosophilae Ashmead (Hymenoptera: Pteromalidae) with 21.5% and Pachycrepoideus vindemmiae (Rondani, 1875) with 46.8%, respectively.

Multidrug Resistance of Escherichia coli Strains Isolated From Bovine Feces and Carcasses in Northeast Mexico

In this work, the antimicrobial resistance profile of Escherichia coli strains (n = 248) isolated from bovine feces and carcass samples from Tamaulipas, Mexico, was evaluated. Susceptibility to 12 antibiotics conventionally used in human and veterinary treatments was determined according to Clinical and Laboratory Standards Institute guidelines. Genes encoding resistance to tetracycline (tetA and tetB), streptomycin (strA), aminoglycoside (aadA), and β-lactamase (blaTEM and blaSHV) were investigated by PCR. Also, stx1, stx2, eae, bfp, and hlyA encoding virulence factors were determined. Of the isolates, 85.9% were confirmed as E. coli strains. Among the 213 E. coli isolates tested, 94.8% (202/213) showed resistance for at least one antimicrobial, mainly ampicillin (83.0%; 177/213), cephalothin (76.0%; 162/213), and tetracyclines (69.0%; 147/213). In all the other antibiotics tested, the resistance percentage was below 36%. A multidrug-resistant phenotype was found in 72.7% of the tested strains. The presence of the tet gene (tetA or tetB) was detected in 43.1% of the isolates, the strA gene in 17.3%, and aadA1 in 51.6%. The blaTEM and blaSHV genes were found in 10.3 and 0.4% of the isolates, respectively. stx1 was detected in 4.2% of isolates, stx2 in 7.0, and hlyA in 2.8%. The virulence genes, eae and bfp, were not detected in any strain. These results indicate that Tamaulipas food products of bovine origin can be a source of multiresistant E. coli strains for the environment and exposure for consumers.

Recovery and seeds germination of four important tropical forage legumes in bovine dung

This experiment was conducted to evaluate the recovery and survival of tropical forage legume seeds (butterfly pea, stylosanthes, perennial soybean and macrotiloma) subjected through the digestive tract of cattle and to evaluate the germination of the seeds in feces of cattle. A 50 g of seed mixed with 150 g of mineral supplement offered to the cattle. Each cattle was considered as repetition. The bovine feces collected up to 60 hours after eating the seeds, where the seeds separated by sieves, using water, gloves procedure and tweezers. Later, they counted and divided into intact and swollen. For the germination test of the recovered seeds, 75 seeds per replicate were used (25 in natura, 25 intact and 25 swollen seeds, respectively). For the germination test in fecal plates, the bovine feces collected between 12 and 30 hours after eating the seeds. After 120 days, we evaluated the total amount of plants emerged within the study period (between 12 and 30 hours) and the average number of emerged plants per fecal signs. The results showed that cattle are facilitating the dispersion of butterfly pea, macrotyloma and perennial soybean, but not stylosanthes. The highest survival as the average number of germinated plants in fecal plates achieved by macrotyloma, followed by butterfly pea species and perennial soybean. The cattle were efficient in the pasture colonization process, due to the considerable dispersion of the seeds. This dispersion was not observed for seeds of stylosanthes.

Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces

The objective was to verify the antibacterial activity of lemongrass and clove oils against Escherichia coli isolated from poultry feces, Staphylococcus aureus isolated from swine and poultry feces and Salmonella sp. isolated from swine and bovine feces. The antimicrobial activity was evaluated by the disc diffusion test using different concentrations of the oils: 160, 80, 40, 20 and 10 ?l ml -1. The multi-resistance of strains relative to that of conventional antimicrobials was also evaluated by the disk diffusion technique, using Multiple Antibiotic Resistance (MAR) index. The oils were characterized based on the results of chromatographic analyses, of which, analysis of lemongrass has been previously published (AZEVEDO et al., 2016). Lemongrass and clove essential oils presented citral and eugenol as the major compound, respectively. The concentrations of the essential oils had a significant effect on the extent of the growth inhibition halo and the coefficient of determination (r²) was higher than 0.80. Clove essential oil generated the largest zone of inhibition when tested against Escherichia coli and S. aureus from poultry feces and Salmonella sp. from the feces of swine, while lemongrass essential oil presented better results against S. aureus isolated from swine feces and Salmonella sp. from bovine feces. S. aureus and Salmonella sp. were multi-resistant to the antimicrobials tested. It is concluded that the essential oils tested have antimicrobial activity against bacteria isolated from bovine, swine, and poultry feces and that this activity is proportional to the concentration of oils and the microorganisms studied.