Occurrence of Fumonisins in Corn-Based Food Products†

1998 ◽  
Vol 61 (6) ◽  
pp. 704-707 ◽  
Author(s):  
MAURICIO M. CASTELO ◽  
SUSAN S. SUMNER ◽  
LLOYD B. BULLERMAN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Exposure ◽  
High Performance ◽  
Fumonisin B1 ◽  
Food Products ◽  
Immunosorbant Assay ◽  
Enzyme Linked Immunosorbant Assay ◽  
The U.S ◽  
Different Parts

Corn-based food products obtained from commercial outlets in three different parts of the U.S., Maryland, Nebraska, and Arizona were analyzed for total fumonisins by a commercial competitive direct enzyme-linked immunosorbant assay (CD-ELISA) and for fumonisin B1 (FB1) by high-performance liquid chromatography (HPLC). The highest fumonisin concentrations were found in samples collected in Maryland, where all 18 samples were found positive for fumonisins (200 to 7,450 ng/g of food) by CD-ELISA and 15 of the 18 samples (83%) were found positive for FB1 (<75 to 5,916 ng/g) by HPLC. Fumonisins were also detected by CD-ELISA in 14 of 15 samples collected in Arizona, with concentrations ranging from 200 to 1,450 ng/g, but analyses by HPLC showed that only 8 of 15 samples (53%) were positive for FB1 (<75 to 1,565 ng/g of food). Of the 23 samples collected in Nebraska, 20 (87%) were positive for fumonisins (200 to 2,500 ng/g) by CD-ELISA, but only 10 (44%) were positive for FB1 (<75 to 927 ng/g) by HPLC. The highest fumonisin and FB1 concentrations were found in cornmeal samples, ranging up to 7,450 ng/g of cornmeal by CD-ELISA and 5,916 ng/g by HPLC. These findings indicate that there may be a risk of human exposure to fumonisins through the consumption of some corn-based foods.

Applicability of an Enzyme-linked Immunosorbant Assay for Neopterin Detection for Screening of Blood Donations

Pteridines ◽  
1994 ◽  
Vol 5 (2) ◽  
pp. 49-54 ◽  
Author(s):  
Peter Mayersbach ◽  
Roman Augustin ◽  
Harald Schennach ◽  
Dietmar Fuchs ◽  
Ernst R. Werner ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Regression Analysis ◽  
Liquid Chromatography ◽  
Linear Regression ◽  
High Performance ◽  
Blood Donors ◽  
Linear Regression Analysis ◽  
Immunosorbant Assay ◽  
Enzyme Linked Immunosorbant Assay ◽  
Better Than

Summary We have evaluated a new commercially available enzyme-linked immunorsorbant assay for neopterin :or its suitability in the context of screening of voluntary blood donors. The assay was performed on 1040 consecutive blood donors, and compared with radioimmunoassay and. in a fraction of 142 donors . . : Iso with high performance liquid chromatography. On repetitive assays of all donations showing a concentration exceeding 8.0 nmol/L in the initial assay. three of the radioimmunoassay results were identified as gross outliers. No such gross outliers were detected for the enzyme-linked immunosorbant assay. RegarJing the reproducibility of results exceeding a cut-off limit of \0 nmol/L neopterin. the enzyme-linked ;mmunosorbant assay was better than the radioimmunoassay. Moreover. the enzyme-linked immunosorbant assay was slightly superior to radioimmunoassay when both tests were compared with high performance liquid chromatography (based on linear regression analysis. evaluation of frequencies of concentrations bant assay was slightly superior to radioimmunoassay when both tests were compared with high performance liquid chromatography (based on linear regression analysis. evaluation of frequencies of concentrations rations. Its slight superiority compared to the conventional radioimmunoassay likely results from the higher degree of automatization employed.

scholarly journals Stinging Nettle (Urtica simensis) as Potential Resource of Vitamin E

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1815-1815
Author(s):  
Tibebeselassie Keflie
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
High Performance ◽  
Wild Plant ◽  
Stinging Nettle ◽  
Funding Sources ◽  
Potential Resource ◽  
Central Highlands ◽  
Different Parts

Abstract Objectives The aim of the current study was to assay the content of vitamin E in stinging nettle (Urtica simensis) Methods Urtica simensis type of stinging nettle is an indigenous wild plant which is widely growing in different parts of Ethiopia. Samples of leaves were collected from Chacha, one of the central highlands in Ethiopia and portioned into sun dried, shade dried and lyophilized groups. For comparison, samples of leaves were also taken from spinach. Vitamin E family such as tocopherols ((α, β, γ, and λ) and tocotrienols (α, β, γ, and λ) were determined using high performance liquid chromatography (HPLC) at department of Food Biofunctionality, University of Hohenheim, Stuttgart, Germany. Results The results showed that the total tocols of stinging nettle in sun-dried, shade dried, and lyophilized groups were 14.1 ± 1.1 mg, 13.8 ± 1.1 mg and 16.9 ± 1.2 mg per 100 g, respectively. In spinach, this value was 3.04 ± 0.7 mg/100 g. Of all vitamin E family, α- tocopherol was the maximum and identified in shade dried group (16.5 ± 1.2 mg/100 g). As compared to stinging nettle, spinach contained very small amount of α- tocopherol (1.7 ± 0.5 mg/100 g). Conclusions In conclusion, Urtica simensis type of stinging nettle contains considerable amount of tocols and can serve as potential resource of vitamin E. Further research is warranted on the nutritional and medicinal values of Urtica simensis stinging nettle. Funding Sources None.

Determination of Fumonisins B1 and B2 in Herbal Tea and Medicinal Plants in Turkey by High-Performance Liquid Chromatography

2004 ◽  
Vol 67 (8) ◽  
pp. 1782-1786 ◽  
Author(s):  
GÜLDEN Z. OMURTAG ◽  
DUYGU YAZICIOĞILU
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
High Performance ◽  
Fumonisin B1 ◽  
Black Tea ◽  
Herbal Tea ◽  
Two Samples ◽  
Fumonisin B2

The purpose of this study was to measure the potential levels of fumonisin B1 (FB1) and fumonisin B2 (FB2) contamination in several herbal teas and medicinal plants that are consumed regularly in Turkey. FB1 and FB2 were detected using high-performance liquid chromatography with fluorescence detection after derivatization with o-phthaldialdehyde. A total of 115 commercially available herbal tea and medicinal plant samples were analyzed. The recoveries in black tea were 86.9 ± 8.42% for FB1 and 102 ± 6.80% for FB2 spiked with 1 μg/g of each analyte. Similarly, the mean recovery results in lime (linden) for FB1 and FB2 were 85.2 ± 9.76% and 78.6 ± 5.67%, respectively. The minimum detectable amounts for the o-phthaldialdehyde derivatives of FB1 and FB2 were 0.025 μg/g (1 ng injected) and 0.125 μg/g (5 ng), respectively. FB1 was detected in two samples (0.160 and 1.487 μg/g), and FB2 was detected in none of the samples.

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