Applicability of an Enzyme-linked Immunosorbant Assay for Neopterin Detection for Screening of Blood Donations

Pteridines ◽  
1994 ◽  
Vol 5 (2) ◽  
pp. 49-54 ◽  
Author(s):  
Peter Mayersbach ◽  
Roman Augustin ◽  
Harald Schennach ◽  
Dietmar Fuchs ◽  
Ernst R. Werner ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Regression Analysis ◽  
Liquid Chromatography ◽  
Linear Regression ◽  
High Performance ◽  
Blood Donors ◽  
Linear Regression Analysis ◽  
Immunosorbant Assay ◽  
Enzyme Linked Immunosorbant Assay ◽  
Better Than

Summary We have evaluated a new commercially available enzyme-linked immunorsorbant assay for neopterin :or its suitability in the context of screening of voluntary blood donors. The assay was performed on 1040 consecutive blood donors, and compared with radioimmunoassay and. in a fraction of 142 donors . . : Iso with high performance liquid chromatography. On repetitive assays of all donations showing a concentration exceeding 8.0 nmol/L in the initial assay. three of the radioimmunoassay results were identified as gross outliers. No such gross outliers were detected for the enzyme-linked immunosorbant assay. RegarJing the reproducibility of results exceeding a cut-off limit of \0 nmol/L neopterin. the enzyme-linked ;mmunosorbant assay was better than the radioimmunoassay. Moreover. the enzyme-linked immunosorbant assay was slightly superior to radioimmunoassay when both tests were compared with high performance liquid chromatography (based on linear regression analysis. evaluation of frequencies of concentrations bant assay was slightly superior to radioimmunoassay when both tests were compared with high performance liquid chromatography (based on linear regression analysis. evaluation of frequencies of concentrations rations. Its slight superiority compared to the conventional radioimmunoassay likely results from the higher degree of automatization employed.

Occurrence of Fumonisins in Corn-Based Food Products†

1998 ◽  
Vol 61 (6) ◽  
pp. 704-707 ◽  
Author(s):  
MAURICIO M. CASTELO ◽  
SUSAN S. SUMNER ◽  
LLOYD B. BULLERMAN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Exposure ◽  
High Performance ◽  
Fumonisin B1 ◽  
Food Products ◽  
Immunosorbant Assay ◽  
Enzyme Linked Immunosorbant Assay ◽  
The U.S ◽  
Different Parts

Corn-based food products obtained from commercial outlets in three different parts of the U.S., Maryland, Nebraska, and Arizona were analyzed for total fumonisins by a commercial competitive direct enzyme-linked immunosorbant assay (CD-ELISA) and for fumonisin B1 (FB1) by high-performance liquid chromatography (HPLC). The highest fumonisin concentrations were found in samples collected in Maryland, where all 18 samples were found positive for fumonisins (200 to 7,450 ng/g of food) by CD-ELISA and 15 of the 18 samples (83%) were found positive for FB1 (<75 to 5,916 ng/g) by HPLC. Fumonisins were also detected by CD-ELISA in 14 of 15 samples collected in Arizona, with concentrations ranging from 200 to 1,450 ng/g, but analyses by HPLC showed that only 8 of 15 samples (53%) were positive for FB1 (<75 to 1,565 ng/g of food). Of the 23 samples collected in Nebraska, 20 (87%) were positive for fumonisins (200 to 2,500 ng/g) by CD-ELISA, but only 10 (44%) were positive for FB1 (<75 to 927 ng/g) by HPLC. The highest fumonisin and FB1 concentrations were found in cornmeal samples, ranging up to 7,450 ng/g of cornmeal by CD-ELISA and 5,916 ng/g by HPLC. These findings indicate that there may be a risk of human exposure to fumonisins through the consumption of some corn-based foods.

scholarly journals DEVELOPMENT AND VALIDATION OF REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ASSAY METHOD FOR ESTIMATION OF FENOFIBRATE IN TABLET DOSAGE FORM PREPARED USING CRYSTALLO-CO-AGGLOMERATES OF THE DRUG

2021 ◽  
Vol 19 (1) ◽  
pp. 19-28
Author(s):  
SANTOSH GANDHI ◽  
MANGESH BHALEKAR ◽  
RAVINA MUTHA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Hplc Method ◽  
Assay Method ◽  
Reverse Phase ◽  
Rp Hplc ◽  
Specificity And Sensitivity ◽  
Tablet Dosage

The aim of the present study was to develop a simple isocratic reverse phase-high performance liquid chromatography (RP-HPLC) method and validate for the determination of fenofibrate in tablet dosage forms. RP-HPLC method was developed using Hi Q Sil C18 (250 cm × 4.6 mm, 5 μm) and mobile phase comprising 1 mM ammonium acetate buffer: Acetonitrile (10:90 v/v) at a flow rate of 1.0 mL/min. The detection was carried out at 290 nm. The retention time was found to be 6.15 ± 0.03 min. Validation of the method was performed for precision, accuracy, linearity, robustness, specificity and sensitivity to conform to the International Conference on Harmonization (ICH) guidelines. The data of linear regression analysis indicated a good linear response in the concentration range of 5 μg/mL–30 μg/mL with correlation co-efficient (R2) of 0.997. The developed method was found to be simple, sensitive, accurate and repeatable for assay of tablets of fenofibrate prepared using crystallo-co-agglomerates of the drug.

scholarly journals Determination of Some Aldehydes by Using Solid-Phase Microextraction and High-Performance Liquid Chromatography with UV Detection

2007 ◽  
Vol 90 (6) ◽  
pp. 1689-1694 ◽  
Author(s):  
Ashwini Kumar ◽  
Baldev Singh ◽  
Ashok Kumar Malik ◽  
Dhananjay K Tiwary
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solid Phase Microextraction ◽  
High Performance ◽  
Solid Phase ◽  
Detection Limits ◽  
Uv Detection ◽  
New Approach ◽  
Better Than

Abstract A new approach has been developed for the extraction and determination of aldehydes such as veratraldehyde, m-nitrobenzaldehyde, cinnamaldehyde, benzaldehyde, and p-chlorobenzaldehyde by using solid-phase microextraction (SPME) and high-performance liquid chromatography with UV detection (HPLC/UV). The method involves adsorption of the aldehydes on polydimethylsiloxane/divinylbenzenecoated fiber, followed by desorption in the desorption chamber of the SPME-HPLC interface, using acetonitrilewater (70 + 30) as the mobile phase; UV detection was at 254 nm. A good separation of 5 aldehydes was obtained on a C18 column. The detection limits of veratraldehyde, m-nitrobenzaldehyde, cinnamaldehyde, benzaldehyde, and p-chlorobenzaldehyde are 25, 41, 13, 12, and 11 pg/mL, respectively, which are about 100 times better than the detection limits for other SPME methods using gas chromatography. The proposed method was validated by determining benzaldehyde in bitter almonds and cinnamaldehyde in cinnamon bark. The recoveries of the 5 analytes were determined by analysis of spiked drinking water.

Evaluation and comparison of a radiative energy attenuation method for determining cholesterol in serum.

1985 ◽  
Vol 31 (4) ◽  
pp. 605-608 ◽  
Author(s):  
P L Cary ◽  
C A Johnson ◽  
P D Whitter ◽  
J W Parker
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Radiative Energy ◽  
Comparison Methods ◽  
Analytical Recovery ◽  
Wide Range

Abstract Determination of cholesterol by a radiative energy attenuation (REA) technique was evaluated and compared with results obtained by the Boehringer Mannheim High Performance Cholesterol Assay and the Du Pont aca. Within-assay and between-assay CVs for the REA method, for two sets of controls, were both less than 5%. We observed no interference with lipemic samples. Analytical recovery averaged 102.8%. We used all three methods for parallel determinations of 217 patients' samples containing a wide range of cholesterol concentrations. Linear regression analysis of the REA results vs those of the comparison methods were as follows: REA = 1.03 Boehringer - 0.072 (r = 0.993) and REA = 1.02 aca - 0.048 (r = 0.995). We also discuss bilirubin interference with the REA method for cholesterol.

scholarly journals Lactose quantification in UHT milk by high-performance liquid chromatography and cryoscopy (freezing point depression)

2021 ◽  
Vol 10 (15) ◽  
pp. e454101523224
Author(s):  
Cleiver Júnio Martins Costa ◽  
Camila Alves Moreira ◽  
Ricardo Corrêa de Santana ◽  
Amado Jésus Silva ◽  
Juliana Karla de Souza Teixeira Almeida ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Linear Regression ◽  
High Performance ◽  
Freezing Point ◽  
Lactose Hydrolysis ◽  
Freezing Point Depression ◽  
Uht Milk ◽  
Hydrolysis Process ◽  
Lactose Concentration

Due to the large number of people with lactose maldigestion, the dairy industries have increased production and diversity of low lactose and lactose-free foods. Consequently, the need to control the lactose hydrolysis process has also risen. This study aimed to correlate freezing point depression (cryoscopy) and lactose concentration, quantified by high-performance liquid chromatography (HPLC), in UHT milk. To accomplish this, UHT milk samples were subjected to seven lactose hydrolysis treatments, using lactase enzyme, resulting in different lactose concentrations. All samples were subjected to HPLC analysis and freezing point measurement, using a cryoscope. The results were plotted on a graph and a linear regression was performed. There was a strong correlation between lactose concentration and freezing point (R = 0,9973) and the coefficient of determination (R2) was 0,9946, which means that 99,46% of the variability of the response data is explained by the linear regression model. Therefore, the results point to the feasibility of estimating the lactose concentration in milk during the hydrolysis process for the production of low lactose milk, by cryoscopy, a quick analysis, with lower cost compared to HPLC and that is already among the analyses commonly performed in dairy industries.

scholarly journals Development and validation of HPTLC method for the analysis of Olmutinib in Bulk drug

2020 ◽  
Vol 13 (1) ◽  
pp. 37-43
Author(s):  
Balu S. Khandare
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Solvent System ◽  
Average Recovery ◽  
Bulk Drug ◽  
Development And Validation ◽  
Hptlc Method

This paper describes a simple, precise, rapid and accurate high- performance thin layer chromatographic (HPTLC) method for determination of Olmutinib in bulk drug. Chroma to graphic separation was per formed on aluminium plates precoated with silicagel60F254 as the stationary phase using solvent system consisted of chloro form: methanol: (9:1v/v). After the application of bands using CAMAG Automatic TLC Sampler 4, the plate was developed in the solvent system up to 70 mm in CAMAGT win Trough Chamber. This solvent system was found to give compact spot for Olmutinib with Rfvalue of 0.32±0.02. The spots were scanned at 267.68nm. The calibration curves were linear with co-relation coefficient of 0.995 for Olmutinib. Linear regression analysis showed good linearity in the concentration range of 100- 1100 ng per spot. The method was validated in terms of Precision, specificity, and Linearity. The average recovery of the standards in the samples was found to be 99.65% at the same time we have checked the C.V. values of Reproducibility, intra-day and inter-day tabulated further. The proposed method can be successfully applied to determine the drug content of bulk drug.

Abbott radiative energy attenuation method for quantifying ethanol evaluated and compared with gas-liquid chromatography and the Du Pont aca.

1984 ◽  
Vol 30 (11) ◽  
pp. 1867-1870 ◽  
Author(s):  
P L Cary ◽  
P D Whitter ◽  
C A Johnson
Keyword(s):  
Regression Analysis ◽  
Liquid Chromatography ◽  
Linear Regression ◽  
Linear Regression Analysis ◽  
Method Comparison ◽  
Cross Reactivity ◽  
Radiative Energy ◽  
Gas Liquid Chromatography ◽  
Analytical Recovery ◽  
Wide Range

Abstract Quantification of ethanol by a radiative energy attenuation (REA) technique was evaluated and compared with results by gas-liquid chromatography (GLC) and by the Du Pont aca. Within-assay CVs were less than 5.5%. Between-assay CVs ranged from 1.9% to 6.0% for serum and blood controls at concentrations of 0.5, 1.0, and 2.5 g/L. We observed no cross reactivity with methanol, isopropanol, or acetone, and analytical recovery of ethanol from serum averaged 101%. For the three-method comparison we performed parallel determinations of 156 blood, 92 serum, and 54 urine samples containing a wide range of ethanol concentrations. Linear regression analysis of the REA results vs those of GLC or aca yielded the following: for serum, REA = 1.03 GLC -0.03 (r = 0.998), REA = 1.13 aca -0.04 (r = 0.999); for blood, REA = 0.97 GLC + 0.05 (r = 0.994), REA = 0.99 aca + 0.06 (r = 0.996); and for urine, REA = 1.01 GLC -0.03 (r = 0.998). We also discuss the clinical and forensic use of the REA method for ethanol.

Stability of Famotidine in Minibags Refrigerated and/or Frozen

DICP ◽  
1989 ◽  
Vol 23 (2) ◽  
pp. 132-135 ◽  
Author(s):  
Linda S. Bullock ◽  
Joseph F. Fitzgerald ◽  
Melvin R. Glick
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Linear Regression ◽  
Analysis Of Variance ◽  
High Performance ◽  
Time Effect ◽  
Time Intervals ◽  
Percent Recovery ◽  
The Stability ◽  
Visual Changes

The stability of famotidine 200 μg/ml in dextrose 5% injection (D5W) and in NaCl 0.9% (NS) solutions in polyvinyl chloride (PVC) minibags was studied when these solutions were stored refrigerated at 4°C for 14 days, or frozen at −20°C for 28 days and then refrigerated for 14 days. Famotidine concentration was determined in the refrigerated samples immediately after compounding (time 0) and also on days 2, 4, 8, and 14 by high-performance liquid chromatography (HPLC). Famotidine concentration was determined by HPLC in frozen samples at time 0 and days 7, 14, 21, 28, 35, and 42. Solutions were also observed for visual changes and pH was tested at these time intervals. Results of the HPLC famotidine analysis demonstrated 94–107 percent recovery of famotidine in D5W and NS at 14 days in refrigerated samples and 98–100 percent recovery of famotidine in minibags frozen for 28 days then refrigerated for 14 days. Analysis of variance showed no time effect on the concentration of famotidine in refrigerated samples (p = 0.741). Linear regression of the frozen minibag data indicated no time effect. Famotidine 200 μg/ml is stable in dextrose 5% injection and NaCl 0.9% injection when stored in PVC bags at 4°C for 14 days, or when frozen for 28 days and then subsequently refrigerated for 14 days.

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