Factors Associated with Salmonella Prevalence on Pork Carcasses in Very Small Abattoirs in Wisconsin

2009 ◽  
Vol 72 (4) ◽  
pp. 714-721 ◽  
Author(s):  
R. J. ALGINO ◽  
G. A. BADTRAM ◽  
B. H. INGHAM ◽  
S. C. INGHAM
Keyword(s):  
Odds Ratio ◽  
Wash Water ◽  
Plate Count ◽  
Processing Conditions ◽  
Department Of Agriculture ◽  
Factors Associated ◽  
Percent Relative Humidity ◽  
Aerobic Plate Count ◽  
Chilled Pork ◽  
The U.S

The U.S. Department of Agriculture has expressed concern over Salmonella prevalence on pork carcasses. Our objectives were to survey the prevalence of Salmonella on pork carcasses in very small Wisconsin abattoirs, and identify processing conditions and indicator bacteria levels associated with reduced Salmonella prevalence. During April to July 2007, sponge samples were obtained from 181 pork carcasses at 10 Wisconsin abattoirs before carcass washing (carcass half A), and after washing and chilling and before fabrication (carcass half B). Each sample was categorized by whether the carcass was skinned, by wash-water temperature (7 to 43°C), and the duration (1 or 2 days), temperature, and percent relative humidity of chilling. Sponge samples were analyzed qualitatively for Salmonella and quantitatively for Escherichia coli, coliforms, Enterobacteriaceae, and aerobic plate count (APC). Salmonella prevalences on skinned and unskinned prewash carcasses were 11.7 and 8.3%, respectively. Corresponding values for chilled carcasses were 32.0 and 19.5% for 1-day chilled carcasses, and 11.4 and 14.7% for 2-day chilled carcasses. Lower Salmonella prevalence on prewash carcasses was significantly related to lower prewash carcass APC levels (odds ratio = 7.8 per change of 1.0 log CFU/cm2), while lower Salmonella prevalence on chilled carcasses was significantly related to 2-day chilling (odds ratio = 5.2), and chilled-carcass levels of coliforms, Enterobacteriaceae, and APC (odds ratio = 1.5 to 1.9 per change of 1.0 log CFU/cm2). Salmonella prevalence on chilled pork carcasses in very small Wisconsin plants could be reduced by chilling carcasses 2 days before fabrication and improving carcass-handling hygiene.

Contamination Revealed by Indicator Microorganism Levels during Veal Processing

2016 ◽  
Vol 79 (8) ◽  
pp. 1341-1347 ◽  
Author(s):  
JOSEPH M. BOSILEVAC ◽  
RONG WANG ◽  
BRANDON E. LUEDTKE ◽  
TOMMY L. WHEELER ◽  
MOHAMMAD KOOHMARAIE
Keyword(s):  
Escherichia Coli ◽  
Plate Count ◽  
Department Of Agriculture ◽  
E Coli ◽  
Veal Calves ◽  
Indicator Microorganism ◽  
Analysis Of Results ◽  
Aerobic Plate Count ◽  
Inspection Service ◽  
The U.S

ABSTRACT During site visits of veal processors, the U.S. Department of Agriculture, Food Safety Inspection Service (FSIS) has reported processing deficiencies that likely contribute to increased levels of veal contamination. Here, we report the results of measuring aerobic plate count bacteria (APC), Enterobacteriaceae, coliforms (CF), and Escherichia coli during eight sample collections at five veal processors to assess contamination during the harvest of bob veal and formula-fed veal before (n = 5 plants) and after (n = 3 plants) changes to interventions and processing practices. Hides of veal calves at each plant had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 6.02 to 8.07, 2.95 to 5.24, 3.28 to 5.83, and 3.08 to 5.59, respectively. Preintervention carcasses had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 3.08 to 5.22, 1.16 to 3.47, 0.21 to 3.06, and −0.07 to 3.10, respectively, before and 2.72 to 4.50, 0.99 to 2.76, 0.69 to 2.26, and 0.33 to 2.12, respectively, after changes were made to improve sanitary dressing procedures. Final veal carcasses had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 0.36 to 2.84, −0.21 to 1.59, −0.23 to 1.59, and −0.38 to 1.45 before and 0.44 to 2.64, −0.16 to 1.33, −0.42 to 1.20, and −0.48 to 1.09 after changes were made to improve carcass-directed interventions. Whereas the improved dressing procedures resulted in improved carcass cleanliness, the changes to carcass-directed interventions were less successful, and veal processors are urged to use techniques that ensure uniform and consistent delivery of antimicrobials to carcasses. Analysis of results comparing bob veal to formula-fed veal found bob veal hides, preintervention carcasses, and final carcasses to have increased (P < 0.05) APC, Enterobacteriaceae, CF, and E. coli (with the exception of hide Enterobacteriaceae; P > 0.05) relative to formula fed veal. When both veal categories were harvested at the same plant on the same day, similar results were observed. Since identification by FSIS, the control of contamination during veal processing has started to improve, but challenges still persist.

Quantitative Comparison of Two Enrichment Methods for Isolating Listeria monocytogenes from Seafoods

1991 ◽  
Vol 54 (1) ◽  
pp. 7-11 ◽  
Author(s):  
JOSEPH LOVETT ◽  
DAVID W. FRANCIS ◽  
JAMES T. PEELER ◽  
ROBERT M. TWEDT
Keyword(s):  
Listeria Monocytogenes ◽  
Drug Administration ◽  
Clinical Sample ◽  
Quantitative Comparison ◽  
Plate Count ◽  
Department Of Agriculture ◽  
Standard Procedures ◽  
Aerobic Plate Count ◽  
The U.S ◽  
Enrichment Methods

Two enrichment methods that had been used as standard procedures by the U.S. Food and Drug Administration (FDA) and the U.S. Department of Agriculture (USDA) were quantitatively compared for their ability to isolate Listeria monocytogenes from seafoods. Cultures of a clinical sample and a seafood isolate were inoculated into raw and cooked shrimp; cultures heated at 57.8°C for 5 min were added to surimi, cooked crabmeat, and cooked shrimp. With the FDA procedure, which used enrichment intervals of 24 h, 48 h, and 7 d, KOH culture treatment and enrichment for 24 h provided no advantage for Listeria recovery. The FDA procedure isolated heated L. monocytogenes from seafoods at a lower level than the USDA method; however, the two methods isolated unheated cells equally well. The greater selectivity of the USDA procedure may offer an advantage for isolating nonheat-stressed Listeria when the aerobic plate count of the product is high.

scholarly journals Effects of Chilling Rate on Outgrowth of Clostridium perfringens Spores in Vacuum-Packaged Cooked Beef and Pork

2003 ◽  
Vol 66 (3) ◽  
pp. 501-503 ◽  
Author(s):  
ROBERT J. DANLER ◽  
ELIZABETH A. E. BOYLE ◽  
CURTIS L. KASTNER ◽  
HARSHAVARDHAN THIPPAREDDI ◽  
DANIEL Y. C. FUNG ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Water Bath ◽  
Department Of Agriculture ◽  
Circulating Water ◽  
Poultry Products ◽  
Heat Treated ◽  
Total Growth ◽  
Chilled Pork ◽  
The U.S

Cooked, chilled beef and cooked, chilled pork were inoculated with three strains of Clostridium perfringens (NCTC 8238 [Hobbs serotype 2], NCTC 8239 [Hobbs serotype 3], and NCTC 10240). Inoculated products were heated to 75°C, held for 10 min in a circulating water bath to heat activate the spores, and then chilled by circulating chilled brine through the water bath. Samples were chilled from 54.4 to 26.6°C in 2 h and from 26.6 to 4.4°C in 5 h. Differences in initial C. perfringens log counts and log counts after chilling were determined and compared with the U.S. Department of Agriculture (USDA) stabilization guidelines requiring that the chilling process allow no more than 1 log total growth of C. perfringens in the finished product. This chilling method resulted in average C. perfringens increases of 0.52 and 0.68 log units in cooked beef and cooked pork, respectively. These log increases were well within the maximum 1-log increase permitted by the USDA, thus meeting the USDA compliance guidelines for the cooling of heat-treated meat and poultry products.

SporadicSalmonella entericaserotype Javiana infections in Georgia and Tennessee: a hypothesis-generating study

2009 ◽  
Vol 138 (3) ◽  
pp. 340-346 ◽  
Author(s):  
L. S. CLARKSON ◽  
M. TOBIN-D'ANGELO ◽  
C. SHULER ◽  
S. HANNA ◽  
J. BENSON ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Disease Surveillance ◽  
Population Based ◽  
Well Water ◽  
Surveillance Programme ◽  
Factors Associated ◽  
Exposure Data ◽  
Animal Contact ◽  
The U.S ◽  
Population Based Survey

SUMMARYFrom 1996 to 2004, the incidence ofSalmonellaJaviana infections increased in FoodNet, the U.S. national active foodborne disease surveillance programme. Contact with amphibians and consumption of tomatoes have been associated with outbreaks ofS. Javiana infection. To generate and test hypotheses about risk factors associated with sporadicS. Javiana infections, we interviewed patients with laboratory-confirmedS. Javiana infection identified in Georgia and Tennessee during August–October 2004. We collected data on food and water consumption, animal contact, and environmental exposure from cases. Responses were compared with population-based survey exposure data. Seventy-two of 117 identifiedS. Javiana case-patients were interviewed. Consumption of well water [adjusted odds ratio (aOR) 4·3, 95% confidence interval (CI) 1·6–11·2] and reptile or amphibian contact (aOR 2·6, 95% CI 0·9–7·1) were associated with infection. Consumption of tomatoes (aOR 0·5, 95% CI 0·3–0·9) and poultry (aOR 0·5, 95% CI 0·2–1·0) were protective. Our study suggests that environmental factors are associated withS. Javiana infections in Georgia and Tennessee.

Histamine-Related Hygienic Qualities and Bacteria Found in Popular Commercial Scombroid Fish Fillets in Taiwan

2004 ◽  
Vol 67 (2) ◽  
pp. 407-412 ◽  
Author(s):  
YUNG-HSIANG TSAI ◽  
HSIEN-FENG KUNG ◽  
TSONG-MING LEE ◽  
GUO-TAI LIN ◽  
DENG-FWU HWANG
Keyword(s):  
Escherichia Coli ◽  
Selective Medium ◽  
Plate Count ◽  
Average Content ◽  
Bacterial Strains ◽  
Allowable Limit ◽  
Fish Fillets ◽  
Southern Taiwan ◽  
Aerobic Plate Count ◽  
The U.S

To determine the histamine-relatedhygienic qualities and bacteria of scombroid fish fillets sold in traditionalretail markets, 61 samples were collected from northern and southern Taiwan. It was found that the content of volatile base nitrogen in most samples was below 25 mg/100 g, which is the regulatory level in Taiwan. The ratio of unacceptable samples/total samples for aerobic plate count and Escherichia coli was 100% and 15% in northern samples and 100% and 20% in southern samples, respectively, compared with the requirements of hygienic standards. The average content of various biogenic amines in all samples were lower than 3 mg/100 g, except for histamine average content (4.6 mg/100 g) in southern samples. Among southern samples, four samples contained 12.8 to 28.8 mg/100 g histamine, which is more than 5 mg/100 g that is the allowable limit suggested by the U.S. Food and Drug Administration. Furthermore, 14 bacterial strains were isolated from sailfish fillets on a selective medium for histamine-forming bacteria. These presumptive histamine-forming strains, such as Proteus, Enterobacter, Klebsiella, Rahnella, and Acinetobacter, have been identified and found to produce 20 to 2,000 ppm histamine after incubating at 37°C for 24 h.

Demographic factors associated with missed follow-up among solid tumor patients treated at a large multi-site academic institution

2020 ◽  
Vol 16 (32) ◽  
pp. 2635-2643
Author(s):  
Samantha L Freije ◽  
Jordan A Holmes ◽  
Saleh Rachidi ◽  
Susannah G Ellsworth ◽  
Richard C Zellars ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Odds Ratio ◽  
Regression Models ◽  
Tumor Patients ◽  
Logistic Regression Models ◽  
Factors Associated ◽  
Follow Up Care ◽  
Risk Patients ◽  
African American Race

Aim: To identify demographic predictors of patients who miss oncology follow-up, considering that missed follow-up has not been well studies in cancer patients. Methods: Patients with solid tumors diagnosed from 2007 to 2016 were analyzed (n = 16,080). Univariate and multivariable logistic regression models were constructed to examine predictors of missed follow-up. Results: Our study revealed that 21.2% of patients missed ≥1 follow-up appointment. African–American race (odds ratio [OR] 1.33; 95% CI: 1.17–1.51), Medicaid insurance (OR 1.59; 1.36–1.87), no insurance (OR 1.66; 1.32–2.10) and rural residence (OR 1.78; 1.49–2.13) were associated with missed follow-up. Conclusion: Many cancer patients miss follow-up, and inadequate follow-up may influence cancer outcomes. Further research is needed on how to address disparities in follow-up care in high-risk patients.

Pyruvate as an Indicator of Quality in Grading Nonfat Dry Milk1

1982 ◽  
Vol 45 (6) ◽  
pp. 561-565 ◽  
Author(s):  
R. T. MARSHALL ◽  
Y. H. LEE ◽  
B. L. O'BRIEN ◽  
W. A. MOATS
Keyword(s):  
Geometric Mean ◽  
Regression Line ◽  
Skim Milk ◽  
Plate Count ◽  
Department Of Agriculture ◽  
Standard Plate Count ◽  
Geometric Average ◽  
Dry Milk ◽  
Standard Plate ◽  
Microscopic Count

Samples of skim milk and nonfat dry milk (NDM) made from it were collected, paired and tested for pyruvate concentration, [P], and Direct Microscopic count (DMC). The skim milk was tested for Standard Plate Count (SPC) and Psychrotrophic Plate Count (PPC). The geometric average DMC of skim milk was more than three times higher than that of the paired NDM samples. However, [P] of NDM was not significantly different from that of the skim milk. Although [P] of skim milk was poorly correlated with SPC and PPC, r = .31 and .26, respectively, it was relatively well correlated with DMC, r = .64. Data were widely dispersed around the regression line when [P] was ≤ 4.0 mg/L. However, [P] increased rapidly when DMCs were > 106/ml. A limit of 10 mg/L of [P] in NDM reconstituted 1:9 was chosen to represent the current U.S. Department of Agriculture Standard for DMC in NDM. This limit failed to classify about 10% of the samples correctly, assuming that each geometric mean DMC was correct. However, the probability that samples meeting the DMC standard would be rejected by the pyruvate test was quite low and the probability was moderate that samples which would be acceptable by the pyruvate test would be rejected by the DMC. For the latter, 28% of the samples having DMCs of ≥ 107/ml contained < 10 mg/L of pyruvate. No sample having ≥ 10 mg/L of pyruvate had a DMC of ≤ 107/ml. Pyruvate concentration in NDM did not change during storage at 5 or 32°C for 90 days.

scholarly journals Examining the Role of Acculturation in E-Cigarette Use among U.S. Immigrant Adults

2021 ◽  
Vol 18 (7) ◽  
pp. 3658
Author(s):  
Sunday Azagba ◽  
Lingpeng Shan
Keyword(s):  
Host Country ◽  
Odds Ratio ◽  
Multivariable Analysis ◽  
Population Data ◽  
Cultural Norms ◽  
Cigarette Use ◽  
Length Of Residence ◽  
Native Populations ◽  
The U.S

Evidence suggests that as immigrants’ length of residence in the host country increases, they may integrate their behavior and norms to align with the new community’s cultural norms. The current study examined e-cigarette use among immigrants in the U.S., and whether the length of residence in the U.S. is associated with e-cigarette use among immigrants compared to the native-born population. Data were drawn from the 2014/15 and 2018/19 Tobacco Use Supplement to the Current Population Survey. Multivariable logistic regression was used to compare differences in e-cigarette use between native-born populations and immigrants, when immigrants’ length of residence in the U.S. was considered. Among immigrants, the prevalence of ever and current e-cigarette use increased significantly from 2.5% and 0.5% in 2014/2015 to 3.2% and 0.8% in 2018/2019, respectively. Multivariable analysis showed that immigrants had significantly lower odds of ever e-cigarette use compared to the mainland-born citizen (0–5 years in the U.S., adjusted Odds Ratio (aOR) 0.57, 95% Confidence Interval (CI) 0.46–0.69; 6–10 years, aOR 0.51, 95% CI 0.41–0.63; 11–20 years, aOR 0.45, 95% CI 0.39–0.53; 20+years, aOR 0.68, 95% CI 0.62–0.76). Similar results were found for current e-cigarette use, with immigrants being less likely to be current users. Findings that e-cigarette use among all immigrants—regardless of years living in the U.S.—was consistently lower than among the native-born population run contrary to the notion that as length of stay increases, health behaviors between immigrants and native populations of the host country become similar.

scholarly journals Use of catheter with 2-methacryloyloxyethyl phosphorylcholine polymer coating is associated with long-term availability of central venous port

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuuki Iida ◽  
Kumiko Hongo ◽  
Takanobu Onoda ◽  
Yusuke Kita ◽  
Yukio Ishihara ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Polymer Coating ◽  
Venous Access ◽  
Preventive Effect ◽  
Central Venous ◽  
Factors Associated ◽  
Central Venous Port ◽  
Venous Port ◽  
Venous Access Device

AbstractCentral venous port (CVP) is a widely used totally implantable venous access device. Recognition of risks associated with CVP-related complications is clinically important for safe, reliable, and long-term intravenous access. We therefore investigated factors associated with CVP infection and evulsion, including the device type. A total of 308 consecutive patients with initial CVP implantation between January 2011 and December 2017 were retrospectively reviewed, and the association of clinical features with CVP-related complications were analyzed. Intraoperative and postoperative complications occurred in 11 (3.6%) and 39 (12.7%) patients, respectively. The overall rate of CVP availability at six months was 91.4%. Malignancy and 2-Methacryloyloxyethyl phosphorylcholine (MPC) polymer-coated catheter use were negatively associated with the incidence of CVP infections. Accordingly, malignancy and MPC polymer-coated catheter use were independent predictors for lower CVP evulsion rate (odds ratio, 0.23 and 0.18, respectively). Furthermore, both factors were significantly associated with longer CVP availability (hazard ratio, 0.24 and 0.27, respectively). This retrospective study identified factors associated with CVP-related complications and long-term CVP availability. Notably, MPC polymer-coated catheter use was significantly associated with a lower rate of CVP infection and longer CVP availability, suggesting the preventive effect of MPC coating on CVP infection.

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