A preliminary study of dental patterns in panoramic radiography for forensic identification

2017 ◽  
Vol 25 (1) ◽  
pp. 75-81
Author(s):  
Hümeyra Özge Yılancı ◽  
Nursel Akkaya ◽  
Dinçer Göksülük
2018 ◽  
Vol 54 (3) ◽  
pp. 179
Author(s):  
Ahmad Yudianto ◽  
Nola Margaret

To prove that mitochondrial DNA damage is not total or partial, as has been found in the preliminary study, studies need to be done to determine the opportunity of successful use of the mitochondrial DNA mini-primer set in an amplicon product below 250 bp. This is important because it can overcome quality problems in degraded DNA, which will complicate the process of DNA forensic identification. This was an observational analytic study with cross sectional design. The study material was DNA from blood and sweat stains taken from abandoned bodies. Samples consisted of 24 pieces of blood and sweat spots. The measurements of mean DNA levels and sample purity used UV-Visible Spectrophotometer, revealing mean DNA in blood samples of 152.89 ± 85.71 µg/ml and sweat samples of 89.19 ± 5.58 µg/ml, and sample purity of DNA  and sweat were 1.89 ± 0.71 and 1.69 ± 0.76. Whereas, the result of D-Loop mtDNA: D-Loop I 143bp nt: 16268 -16410 and D-Loop HVS II 126bp nt: 34 -159, indicating blood spots were detected positively >95% and sweat was detected positively in 5%-20%. Results of DNA sequencing from mtDNA of blood spots and sweat spots in 126 bp and 143 bp amplicon revealed nucleotide damage marked with the letter 'N'. In conclusion, mini-primers of mitochondrial DNA in the amplification product mtDNA D-Loop HVS II 126 bp (nt 59-134) and D-Loop HVS I 143 bp (nt 16268-16410) were effectively used as support for DNA profiling in forensic medicine.


Author(s):  
Marina González ◽  
Kristiane Mariotti ◽  
Adriano Gomes ◽  
Marcos Ferrão ◽  
Renata Limberger

The temporal estimation of the fingermarks deposition at crime scenes is a recurring issue in forensic identification. To study this challenging topic, a preliminary study was proposed to develop a method of fingermark analysis by Fourier Transform Infrared Microscopy (µ-FTIR), using chemometric tools for time separation in a six-day aging study. The samples were collected and analyzed at hour zero, 3 days (72 hours), 4 days (96 hours), 5 days (120 hours) and 6 days (144 hours). The samples were separated into a calibration set and a test set, using Kennard Stone. Following, a comparison between variable selection tools was made of the Ant of Colony (AOC) and the Genetic Algorithm (GA) were used with subsequent application of the Linear Discriminant Analysis (LDA). The results showed that in the analyzed samples there was a predominance of sebaceous material because of the presence of saturated esters signals, with two regions of interest in the infrared spectra, the first being 1800 - 1100 cm-1 and the second region being 3000 - 2800 cm-1. The statistical tools could group the fingermarks by donors and by age, emphasizing the separation within the tested period. More studies need to be carried out, but this work provide that µ-FTIR associated with chemometric analysis was able to separate fingermarks samples aged for up to a week.


2014 ◽  
Vol 22 (4) ◽  
pp. 263-266 ◽  
Author(s):  
C.L. Stefanescu ◽  
M.F. Popa ◽  
L.S. Candea

Author(s):  
John H.L. Watson ◽  
John L. Swedo ◽  
R.W. Talley

A preliminary study of human mammary carcinoma on the ultrastructural level is reported for a metastatic, subcutaneous nodule, obtained as a surgical biopsy. The patient's tumor had responded favorably to a series of hormonal therapies, including androgens, estrogens, progestins, and corticoids for recurring nodules over eight years. The pertinent nodule was removed from the region of the gluteal maximus, two weeks following stilbestrol therapy. It was about 1.5 cms in diameter, and was located within the dermis. Pieces from it were fixed immediately in cold fixatives: phosphate buffered osmium tetroxide, glutaraldehyde, and paraformaldehyde. Embedment in each case was in Vestopal W. Contrasting was done with combinations of uranyl acetate and lead hydroxide.


Author(s):  
H.D. Geissinger ◽  
C.K. McDonald-Taylor

A new strain of mice, which had arisen by mutation from a dystrophic mouse colony was designated ‘mdx’, because the genetic defect, which manifests itself in brief periods of muscle destruction followed by episodes of muscle regeneration appears to be X-linked. Further studies of histopathological changes in muscle from ‘mdx’ mice at the light microscopic or electron microscopic levels have been published, but only one preliminary study has been on the tibialis anterior (TA) of ‘mdx’ mice less than four weeks old. Lesions in the ‘mdx’ mice vary between different muscles, and centronucleation of fibers in all muscles studied so far appears to be especially prominent in older mice. Lesions in young ‘mdx’ mice have not been studied extensively, and the results appear to be at variance with one another. The degenerative and regenerative aspects of the lesions in the TA of 23 to 26-day-old ‘mdx’ mice appear to vary quantitatively.


Author(s):  
J P Cassella ◽  
V Salih ◽  
T R Graham

Left ventricular assist systems are being developed for eventual long term or permanent implantation as an alternative to heart transplantation in patients unsuitable for or denied the transplant option. Evaluation of the effects of these devices upon normal physiology is required. A preliminary study was conducted to evaluate the morphology of aortic tissue from calves implanted with a pneumatic Left Ventricular Assist device-LVAD. Two 3 month old heifer calves (calf 1 and calf 2) were electively explanted after 128 days and 47 days respectively. Descending thoracic aortic tissue from both animals was removed immediately post mortem and placed into karnovsky’s fixative. The tissue was subsequently processed for transmission electron microscopy (TEM). Some aortic tissue was fixed in neutral buffered formalin and processed for routine light microscopy.


2021 ◽  
Author(s):  
Hicham Zaroual ◽  
El Mestafa El Hadrami ◽  
Romdhane Karoui

This study examines the feasibility of using front face fluorescence spectroscopy (FFFS) to authenticate 41 virgin olive oil (VOO) samples collected from 5 regions in Morocco during 2 consecutive crop seasons.


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