Key Issues in Using Molecular Techniques to Improve Conservation and Use of Plant Genetic Resources

Author(s):  
Carmen de Vicente ◽  
Toby Hodgkin ◽  
Geoffrey Hawtin
Author(s):  
Paula Bramel ◽  

This chapter reviews the key issues and challenges facing genebanks in preserving crop genetic diversity ex situ. Local crop genetic diversity is challenged with changes in land use, urbanization, land degradation, changes in agricultural practises, availability of improved varieties, changes in market preference, and the impact of climate change. Efforts have been made to secure plant genetic resources ex situ for future use but there are significant issues related to cost effective, efficient, secure, rational, and sustainable long-term ex situ conservation. It begins by addressing issues for the composition of ex situ collections and moves on to discuss issues for routine operations for conservation. The chapter also highlights issues for the use of conserved genetic resources, before concluding with a summary of why the development of sustainable genebank systems is so important.


2011 ◽  
Vol 9 (2) ◽  
pp. 155-158 ◽  
Author(s):  
Kyujung Van ◽  
Dong Hyun Kim ◽  
Jin Hee Shin ◽  
Suk-Ha Lee

Plant genetic resources (PGR) include cultivars, landraces, wild species closely related to cultivated varieties, breeder's elite lines and mutants. The loss of genetic diversity caused by the practice of agriculture and the availability of genetic information has resulted in a great effort dedicated to the collection of PGR. Prior to the advent of molecular profiling, accessions in germplasm collections were examined based on morphology. The development of molecular techniques now allows a more accurate analysis of large collections. Next-generation sequencing (NGS) with de novo assembly and resequencing has already provided a substantial amount of information, which warrants the coordination of existing databases and their integration into genebanks. Thus, the integration and coordination of genomic data into genebanks is very important and requires an international effort. From the determination of phenotypic traits to the application of NGS to whole genomes, every aspect of genomics will have a great impact not only on PGR conservation, but also on plant breeding programmes.


2013 ◽  
Vol 864-867 ◽  
pp. 2537-2540
Author(s):  
Cheng Zhang ◽  
Zheng Hong Li ◽  
Xuan Zhou ◽  
Hong Dao Zhang ◽  
Ji Lin Li ◽  
...  

For at least the last 10,000 years humans have been selecting plants that performed better for them across a wide range of criteria including fruit size, number of fruit per plant, adaptation to specific environments, resistance to certain pests, and many other characteristics. As the demand for agricultural productivity continues to rise, there is an increasing pressure on plant breeders to develop new varieties that are higher yielding and able to produce in more challenging environments. In recent years, there has been a significant increase in the application of molecular genetic methods for assessing the conservation and use of plant genetic resources that provides the new ways for plant breeding, for example, the high-throughput sequencing technology provides the possibility of analysing high numbers of samples over smaller periods of time. Currently, molecular breeding is standard practice in many crops, and a diverse collection of plant genetic resources and these resources remain accessible to breeders is important.This article describes the exploitation of plant genetic resources and modern plant breeding, introduces the application of molecular techniques in plant breeding and perspectives the significant of development and utilization of plant genetic resources for plant breeding.


Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
JS Sung ◽  
CW Jeong ◽  
YY Lee ◽  
HS Lee ◽  
YA Jeon ◽  
...  

2017 ◽  
Vol 52 (6) ◽  
pp. 1116-1128 ◽  
Author(s):  
N.I. Dzyubenko ◽  
◽  
E.A. Dzyubenko ◽  
E.K. Potokina ◽  
S.V. Bulyntsev ◽  
...  

Author(s):  
N. V. Kulyakina ◽  
G. A. Kuzmitskaya ◽  
T. K. Yurechko

The downy mildew (peronosporosis) is the most dangerous and harmful cucumber disease in the Far-Eastern region. Its harmfulness is the highest during the period of flowering – fruiting, because it may completely ruin the cucumber plants both in open and protected ground. Use of cucumber sorts with effective genes that are resistant to the disease is the most efficient way to fight cucumber peronosporosis. In this connection the publication adduces the facts of study the sorts and hybrids of different ecological and geographical origin from the genetic resources collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in comparison with three zoned sorts of DV NIISH selection – Mig, Khabar, Amurchonok. The aim of researches – to identify the sort samples cucumber according to the main economically valuable traits and also to the degree resistance of genetypes to the main disease of the culture in the region – downy mildew (peronosporosis), for allow to select the source material for the creation of sorts and hybrids, highly productive and resistant to biotic and abiotic stress environmental factors in the monsoon climate conditions of Khabarovsk region. The perspective genetypes having both the resistance to the diseases and productivity are not revealed as a result of the evaluation of the collection of cucumber samples according to their susceptibility by fungus diseases and productivity. Slavyanskiy (Belarus) is more resistant to peronosporosis, especially in 2016 year conditions, although it was inferior to the damage degree of disease to the zoned sorts – Mig, Khabar, Amurchonok. This perspective sample is planned to be used as a source of donors of the main economically valuable traits in a further selective work.


Author(s):  
S.V. Chernobai ◽  
V.K. Riabchun ◽  
T.B. Kapustina ◽  
V.S. Melnyk ◽  
O.E. Shchechenko

Goal. To build up a spring triticale genetic bank to provide breeding, scientific and educational institutions with initial material and to preserve the existing diversity. To update the database of accessions with a set of valuable economic and morphological features. Results and discussion. The methodology and results of the collection formation and evaluation of spring triticale accessions in the National Center for Plant Genetic Resources of Ukraine of Plant Production Institute nd. a V. Ya. Yuriev are presented. The formed collection includes 1,935 accessions from 27 countries: 42 varieties and 1,478 breeding lines from Ukraine, 92 varieties and 248 lines from foreign countries and also 75 genetic lines. The collection was formed by major valuable economic features (plant height, growing season length, spike threshing, yield, 1000-grain weight, disease resistance, technological properties, etc.). Accessions with the majority of morpho-biological and valuable economic features were selected. All the accessions in the collection are certificated. 1,762 accessions were packed for storage into the National Depository; 1,507 of them were packed for long-term storage. Conclusions. The gene pool of spring triticale from the collection of the Gene Bank of Plants of Ukraine is widely used for breeding. This allows conducting hybridization of genetically and ecologically remote forms with various expressions of features and obtaining whole new breeding material. Involvement of collection accessions in breeding allows generating new genetic sources of valuable economic features.


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