Changes of Nutrients in Media and Mycelia on Liquid Spawn Culture of Lentinula edodes

Sorghum biscuits were enriched with mushroom powders (Lentinula edodes, Auricularia auricula and Tremella fuciformis) at 5%, 10% and 15% substitution levels. An in vitro gastrointestinal digestion was used to evaluate the effect of this enrichment on the phenolic content and soluble peptide content as well as antioxidant activities of the gastric or intestinal supernatants (bio-accessible fractions), and the remaining portions of phenolic compounds, antioxidants and β-glucan in the undigested residue (non-digestible fraction). The phenolic content of the gastric and intestinal supernatants obtained from digested mushroom-enriched biscuits was found to be higher than that of control biscuit, and the phenolic content was positively correlated to the antioxidant activities in each fraction (p < 0.001). L. edodes and T. fuciformis enrichment increased the soluble protein content (small peptide) of sorghum biscuits after in vitro digestion. All mushroom enrichment increased the total phenolic content and β-glucan content of the undigested residue and they were positively correlated (p < 0.001). The insoluble dietary fibre of biscuits was positively correlated with β-glucan content (p < 0.001) of undigested residue. These findings suggested that enriching food with mushroom derived dietary fibre increases the bioavailability of the non-digestible β-glucan and phenolic compounds.

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Identification, heterologous production and bioactivity of lentinulin A and dendrothelin A, two natural variants of backbone N-methylated peptide macrocycle omphalotin A

Scientific Reports ◽

10.1038/s41598-021-83106-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Emmanuel Matabaro ◽

Hannelore Kaspar ◽

Paul Dahlin ◽

Daniel L. V. Bader ◽

Claudia E. Murar ◽

...

Keyword(s):

Natural Products ◽

Lentinula Edodes ◽

Gene Clusters ◽

Biosynthetic Gene Cluster ◽

Precursor Protein ◽

Fungal Genome ◽

Homologous Gene ◽

C Terminus ◽

Natural Variants ◽

Recombinant Peptides

AbstractBackbone N-methylation and macrocyclization improve the pharmacological properties of peptides by enhancing their proteolytic stability, membrane permeability and target selectivity. Borosins are backbone N-methylated peptide macrocycles derived from a precursor protein which contains a peptide α-N-methyltransferase domain autocatalytically modifying the core peptide located at its C-terminus. Founding members of borosins are the omphalotins from the mushroom Omphalotus olearius (omphalotins A-I) with nine out of 12 L-amino acids being backbone N-methylated. The omphalotin biosynthetic gene cluster codes for the precursor protein OphMA, the protease prolyloligopeptidase OphP and other proteins that are likely to be involved in other post-translational modifications of the peptide. Mining of available fungal genome sequences revealed the existence of highly homologous gene clusters in the basidiomycetes Lentinula edodes and Dendrothele bispora. The respective borosins, referred to as lentinulins and dendrothelins are naturally produced by L. edodes and D. bispora as shown by analysis of respective mycelial extracts. We produced all three homologous peptide natural products by coexpression of OphMA hybrid proteins and OphP in the yeast Pichia pastoris. The recombinant peptides differ in their nematotoxic activity against the plant pathogen Meloidogyne incognita. Our findings pave the way for the production of borosin peptide natural products and their potential application as novel biopharmaceuticals and biopesticides.

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Antibacterial activity and antibacterial mechanism of ethanol extracts of Lentinula edodes (Shiitake) and Agaricus bisporus (button mushroom)

International Journal of Environmental Health Research ◽

10.1080/09603123.2021.1919292 ◽

2021 ◽

pp. 1-14

Author(s):

Elif Ayşe Erdoğan Eliuz

Keyword(s):

Antibacterial Activity ◽

Agaricus Bisporus ◽

Lentinula Edodes ◽

Antibacterial Mechanism ◽

Button Mushroom ◽

Ethanol Extracts

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Preliminary study on Se-enriched Lentinula edodes mycelium as a proposal of new feed additive in selenium deficiency

PLoS ONE ◽

10.1371/journal.pone.0233456 ◽

2020 ◽

Vol 15 (5) ◽

pp. e0233456 ◽

Cited By ~ 2

Author(s):

Bożena Muszyńska ◽

Ewelina Szacawa ◽

Dorota Bederska-Łojewska ◽

Katarzyna Dudek ◽

Bartosz Pomierny ◽

...

Keyword(s):

Lentinula Edodes ◽

Selenium Deficiency ◽

Feed Additive ◽

Preliminary Study

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Recombinant latcripin 11 of Lentinula edodes C91-3 suppresses the proliferation of various cancer cells

Gene ◽

10.1016/j.gene.2017.10.080 ◽

2018 ◽

Vol 642 ◽

pp. 212-219 ◽

Cited By ~ 3

Author(s):

Yifan Gao ◽

Arshad Ahmed Padhiar ◽

Jia Wang ◽

Wei Zhang ◽

Mintao Zhong ◽

...

Keyword(s):

Cancer Cells ◽

Lentinula Edodes

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The smell and odorous components of dried shiitake mushroom, Lentinula edodes II: sensory evaluation by ordinary people

Journal of Wood Science ◽

10.1007/s10086-004-0689-0 ◽

2005 ◽

Vol 51 (6) ◽

pp. 628-633 ◽

Cited By ~ 8

Author(s):

Masakazu Hiraide ◽

Ichiro Yokoyama ◽

Yoshifumi Miyazaki

Keyword(s):

Sensory Evaluation ◽

Lentinula Edodes ◽

Ordinary People ◽

Shiitake Mushroom ◽

Dried Shiitake Mushroom

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Biological and Molecular Characteristics of a Novel Partitivirus Infecting the Edible Fungus Lentinula edodes

Plant Disease ◽

10.1094/pdis-07-16-0951-re ◽

2017 ◽

Vol 101 (5) ◽

pp. 726-733 ◽

Cited By ~ 15

Author(s):

Mengpei Guo ◽

Yinbing Bian ◽

Jinjie Wang ◽

Gangzheng Wang ◽

Xiaolong Ma ◽

...

Keyword(s):

Lentinula Edodes ◽

Control Strategies ◽

Phylogenetic Analyses ◽

Pcr Analysis ◽

Molecular Characteristics ◽

Rt Pcr ◽

Edible Fungus ◽

Qpcr Analysis ◽

Disease Diagnostics ◽

Wild Strains

A new partitivirus named Lentinula edodes partitivirus 1 (LePV1) was isolated from a diseased L. edodes strain with severe degeneration of the mycelium and imperfect browning in bag cultures. The nucleotide sequences of LePV1 dsRNA-1 and dsRNA-2 were determined; they were 2,382 bp and 2,245 bp in length, and each contained a single ORF encoding RNA-dependent RNA polymerase (RdRp) and coat protein (CP), respectively. The purified virus preparation contained isometric particles 34 nm in diameter encapsidating these dsRNAs. Phylogenetic analyses showed LePV1 to be a new member of Betapartitivirus, with the RdRp sequence most closely related to Grapevine partitivirus. RT-PCR analysis showed that 27 of the 56 Chinese L. edodes core collection strains carry LePV1, with the virus being more common in wild strains than cultivated strains. In addition, qPCR analysis suggested that coinfection with L. edodes mycovirus HKB (LeV-HKB) could increase replication of the RdRp gene of LePV1. This study may be essential for the development of more accurate disease diagnostics and the formulation of control strategies for viral diseases in L. edodes.

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