scholarly journals EFFICACY EVALUATION OF EXTRACTS OF BRASSICA JUNCEA AND BRASSICA ALBA SEEDS AS POTENTIAL ANTIBACTERIAL AGENT-IN VITRO STUDY, AGAINST PYOGENIC MICROBES - STREPTOCOCCUS PYROGENS, STAPHYLOCOCCUS AUREUS AND E.COLI

2021 ◽  
Vol 9 (7) ◽  
pp. 1344-1348
Author(s):  
Seema Nishant Parma ◽  
Madhusudan N Pawar ◽  
Rehman M. K. ◽  
Madhuri Wagh
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Microbial Activity ◽  
Brassica Juncea ◽  
Broad Spectrum ◽  
In Vitro Study ◽  
Vitro Study ◽  
World Health

The World Health Organization (WHO) reported that about 80% of the world’s population depends primarily on traditional medicine.[1] Ayurveda is the science of life, which aims at building a healthy body with a sound mind. During the past few decades, and in recent Covid-19 Pandaemic situations traditional systems of medicines have become a topic of global importance. The number of emerging multidrug-resistant microbial strains is continuously increasing and has become one of the most serious threats to the successful treatment of infectious diseases. In this study, the antibacterial potentials of the extracts of water, Petroleum ether, methanol & Chloroform of Brassica juncea & Brassica alba seeds were tested against mentioned pyogenic bacteria. Given reports of Brassica species in the treatment of various infections since the Vedic period & Ayurveda, the seed oil had been investigated for its anti-microbial activity by several research workers. Some herbs from Rakshoghna Gana [2] were screened for their in vitro anti-microbial activity on E.Coli and Staphylococcus Aureus. Hence as a part of further research, Brassicajuncea and Brassica alba various extracts and crude oils were evaluated for antibacterial activity against pyogenic bacteria Streptococcus aureus, Staphylococcus aureus and E.Coli by Agar Cup Dilution Technique against standard chemotherapeutic agents, by using 12 antibiotics, sensitivity discs. In vitro study revealed out that crude oil of Brassica juncea and Brassica alba is not potent as standard antimicrobial agents, but water extract and methanol extract possess significant antimicrobial activity in Vitro. Both the extract possesses antimicrobial activity against all 3 pyogenic bacteria and potent as standard 12 antimicrobial drugs. The extracts showed a broad spectrum of antibacterial activity, inhibition zones of bacteria strains ranged from 3-12 mm for Staphylococcus aureus,3-15mm for Streptococcus pyrogens 5-12mm for E. coli. The result of the study supports the immense medicinal properties of Brassica juncea which has revealed a significant scope to develop a novel broad spectrum of antibacterial herbal formulation. Again Brassica alba was found superior in antibacterial action as compared to Brassica juncea as its mention in Ayurved Lexicon Bhavprakash Nighantu,[3,4] results substantiate the same. This is further open for re- search workers to carry out further research work on this project and to see the phagocytic action of the drug, fix the dose of the drug, fractionization of water and methanol extracts by HPTLC separation of active principle as further drug development. Keywords: Brassica juncea, Brassica alba, antibacterials agents, herbal medicine.

scholarly journals Antibacterial Activity of Haslea ostrearia Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)

Omni-Akuatika ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 30
Author(s):  
Ikfa Permatasari ◽  
Fiddy Semba Prasetiya ◽  
Sulastri Arsad ◽  
Mochamad Untung Kurnia Agung ◽  
Jean-Luc Mouget
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
In Vitro Study ◽  
Water Soluble ◽  
Vitro Study ◽  
Inhibitory Zone ◽  
Haslea Ostrearia

Haslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical of the cell and extracellular which released into the culture medium. This research aimed to test the bioactivity of Haslea ostrearia supernatant adapted in Indonesia as an antibacterial against pathogenic bacteria relevant to marine culture using in-vitro study. This research was using an explorative method, data analysed statistically and descriptive-comparatively observed. Observation parameters were the inhibitory zone of antibacterial activity, absorbance value of MIC test, and bacterial growth qualitatively from MBC test. Marennine concentration in the supernatant of Haslea ostrearia adapted in Indonesia is about 3.74 mg. L-1. The highest concentration supernatant of Haslea ostrearia that shown antibacterial activity is 3.5 mg. L-1 with inhibitory zone diameter is about 6.87 mm for Staphylococcus aureus and 7.14 mm for Vibrio harveyi, correspondingly. The minimum concentration that inhibits the growth of Staphylococcus aureus is 0.03 mg. L-1, while for Vibrio harveyi is 0.06 mg. L-1. Antibacterial activity of supernatant Haslea ostrearia originally adapted in Indonesia classified into bacteriostatic

scholarly journals Antimicrobial Effect of Newly Formulated Toothpastes and a Mouthrinse on Specific Microorganisms: An In Vitro Study

2019 ◽  
Vol 13 (02) ◽  
pp. 172-177 ◽  
Author(s):  
Yeliz Guven ◽  
Nilufer Ustun ◽  
Elif Bahar Tuna ◽  
Oya Aktoren
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Vitro Study

Abstract Objective The aim of this in vitro study was to assess the antimicrobial properties of newly formulated toothpastes (four toothpastes for adults and two toothpastes for kids/babies) and a mouthrinse. Materials and Methods Newly formulated six different toothpastes and one mouthrinse of a single brand and commercially available five toothpastes and three mouthrinse were investigated for their antimicrobial activity against two oral pathogens, Streptococcus mutans and Candida albicans, by agar well diffusion assay. After incubation, the inhibition zone diameters were measured in millimeters and statistical analyses were performed. Results All experimental adult toothpastes exhibited good antimicrobial activity against S. mutans and C. albicans except the experimental toothpaste D. Experimental toothpaste B exhibited the highest antibacterial activity against C. albicans and S. mutans. Experimental toothpaste for kids showed the best antimicrobial activity against S. mutans when kids’ toothpastes were compared. None of the tested toothpastes for kids/babies showed antibacterial effects for C. albicans. Among the mouthrinse tested, Sensodyne mouthrinse showed the best results. Experimental mouthrinse showed significantly lower antibacterial activity against S. mutans then Sensodyne, Eludril, and chlorhexidine mouthrinse. Conclusion Although experimental toothpaste and mouthrinse formulations revealed good results in terms of antimicrobial activity to some specific microorganisms, further studies involving more bacterial species or analyzing the quality and efficacy of these products by other in vitro or in vivo tests are needed.

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