scholarly journals Teknik Memanen Makrokonidia dari Dermatofita Microsporum gypseum dan Trichophyton mentagrophytes

2020 ◽  
Vol 4 (2) ◽  
pp. 182
Author(s):  
Ana Rufaidah ◽  
Eko Sugeng Pribadi ◽  
I Ketut Mudite Adnyane
Keyword(s):  
Trichophyton Mentagrophytes ◽  
Microsporum Gypseum

scholarly journals Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 737
Author(s):  
Marina Pekmezovic ◽  
Melina Kalagasidis Krusic ◽  
Ivana Malagurski ◽  
Jelena Milovanovic ◽  
Karolina Stępień ◽  
...  
Keyword(s):  
Fungal Growth ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Gypseum ◽  
Medium Chain Length ◽  
Gold Drugs ◽  
Growth Inhibitory Activity ◽  
Transdermal Application ◽  
Antifungal Efficiency

Novel biodegradable and biocompatible formulations of “old” but “gold” drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (~50 µm). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.

scholarly journals Frecuencia de dermatomicosis y factores asociados en población vulnerable de la ciudad de Manizales. Colombia. 2011

2016 ◽  
Vol 18 (6) ◽  
pp. 953
Author(s):  
Gloria Ines Estrada Salazar ◽  
José A. Chacón-Cardona
Keyword(s):  
Candida Albicans ◽  
Trichophyton Rubrum ◽  
Culture Media ◽  
Trichophyton Mentagrophytes ◽  
Direct Analysis ◽  
Microsporum Gypseum ◽  
Epidermophyton Floccosum ◽  
Palabras Clave ◽  
Fusarium Sp ◽  
Cutaneous Mycosis

Resumen:Objetivo: Determinar la frecuencia de las dermatomicosis en personas de diferentes instituciones de atención social en la ciudad de Manizales durante el año 2011. Método: Mediante la toma de muestras de los sitios que presentaban algún tipo de lesión sospechosa de ser una micosis cutánea, se hizo un análisis directo con KOH y cultivo en medios de Saboureaud y Mycosel. Se aplicó un instrumento de recolección de información para establecer  factores asociados con la presencia de estos microorganismos.Resultados: Los hongos levaduriformes encontrados con mayor frecuencia fueron: Candida albicans, Trichosporon sp, y los mohos saprofitos Penicillium sp, fusarium sp; seguido de hongos dermatofitos como: Trichophyton mentagrophites, Trichophyton rubrum, Epidermophyton floccosum y Microsporum gypseum. Las lesiones secas y descamativas se encontraron con mayor frecuencia. El compartir baños y vivir en hacinamiento y el uso de elementos comunes fueron los factores asociados más importantes en este estudio.Conclusiones. Las dermatomicosis son frecuentes en poblaciones vulnerables y se asocian a diferentes factores muy similares a los encontrados en otros estudios de igual naturaleza.    Palabras clave: dermatomicosis, onicomicosis, jóvenes, ancianos, factores asociados. Summary:Objective: To determine the frequency of ringworm in people of different social care institutions in the city of Manizales in 2011.Method: Using the sampling sites that had some kind of suspicious lesion from a cutaneous mycosis, direct analysis with KOH and culture media was Saboureaud and Mycosel. An instrument of data collection was used to establish factors associated with the presence of these microorganisms.Results: The yeast found most frequently were: Candida albicans, Trichosporon sp and Penicillium molds saprophytes sp, Fusarium sp, followed by dermatophyte fungi such as Trichophyton mentagrophytes, Trichophyton rubrum, Epidermophyton floccosum and Microsporum gypseum. Dry scaly lesions were found more frequently. The shared bathrooms and living in overcrowded and the use of common elements were the most important in this study associated factors.Conclusions: Dermatomycoses are common in vulnerable populations and are associated with different very similar to those found in other studies of the same nature factors. Keywords: ringworm, onychomycosis, young, old, associated factors.

scholarly journals Dermatofitosis humana en una comunidad con desigualdad social, Ayacucho 2018

2019 ◽  
Vol 27 (1) ◽  
Author(s):  
Serapio Romero Gavilán
Keyword(s):  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Microsporum Gypseum

Trabajo de investigación desarrollado con el objetivo de conocer la frecuencia de la dermatofitosis humana en una comunidad con desigualdad social. Hipótesis: la dermatofitosis humana es una afección fúngica muy frecuente en comunidades con desigualdad social. Zona de estudio: comunidad con desigualdad social periurbana de la ciudad de Ayacucho. Tipo de estudio: no experimental. Diseño de estudio: descriptivo-transversal. Muestra: no probabilística, individuos con signos de afecciones compatibles a micosis superficial. Metodología: muestras de escamas de piel, pelos, fragmentos de uñas de pies y manos, escamas de planta, espacios interdigitales y otras partes de cuerpo, fueron tomadas con una hoja de bisturí de filo romo o con el borde de un portaobjetos previa desinfección con alcohol al 70% y colocadas en sobres de papel oscuro etiquetados, en el laboratorio de Epidemiología y Micología de la Universidad Nacional de San Cristóbal de Huamanga, se realizó la observación directa con KOH al 20% y la siembra en placas Petri conteniendo Agar Sabouraud y Agar selectivo para hongos patógenos, después de una incubación a 25ºC por hasta 14 días; las colonias coincidentes con dermatofitos fueron observados al microscopio para identificarlos. Resultados: se ha encontrado que 85/153 (55,5%) presentaron diversas formas de dermatofitosis, no se observó preferencia de la dermatofitosis humana con relación al género (p> 0,05), los factores asociados a la dermatofitosis determinados estadísticamente (p< 0,05) fueron la higiene, el piso de la vivienda y la crianza de animales, se han identificado las especies de Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis y Microsporum gypseum.

scholarly journals ANTIDERMATOPHYTIC ACTIVITY OF STREPTOMYCES CACAOI SUBSP. CACAOI. M20

2017 ◽  
Vol 10 (7) ◽  
pp. 300
Author(s):  
Janaki T
Keyword(s):  
Spectral Analysis ◽  
Volatile Compounds ◽  
Food Poisoning ◽  
Trichophyton Mentagrophytes ◽  
Diffusion Method ◽  
Microsporum Gypseum ◽  
Sequencing Analysis ◽  
Human Welfare ◽  
Agar Plug ◽  
Antidermatophytic Activity

 Objective: This study is an approach to find the alternative drug for the dermatophytosis because some of the existing antibiotics cause toxicity and also found expensive. Methods: There were 25 actinomycetes isolated from the soil sample of the mangrove, Avicennia marina from Ariyankuppam backwater area, Puducherry. Among the 25 isolates, the most active isolate, M20 was selected initially based on its antifungal activity, and it was checked for antidermatophytic activity against three dermatophytes, Microsporum gypseum, Trichophyton mentagrophytes, and Epidermophyton floccosum by agar plug method, well diffusion method followed by food poisoning technique. Release of volatile compounds from the isolate M20 has also been tested. Ultraviolet (UV)-vis spectral analysis of partially purified yellow compound fraction was done. Results: The isolate M20 actively controlled the growth of dermatophytes by agar plug method in the primary screening, well diffusion method in the secondary screening. The isolate was identified as Streptomyces cacaoi sub sp. cacaoi by 16sRNA sequencing analysis. The 10% (10 ml) culture filtrate of the isolate M20 was found to control the radial growth of these three dermatophytic fungi by food poisoning technique. Volatile compounds of the isolate M20 affected the mycelial growth of M. gypseum tremendously. Complete arrest of growth of M. gypseum was noticed with the combination of both volatile and non-volatile antibiotic compound of the isolate M20. The partially purified compound fraction (yellow) (0.5 mg/6 mm disc) inhibited the growth of M. gypseum (12 mm), and it was compared with the standard antibiotic clotrimazole (0.5 mg/6 mm disc), whose inhibition on M. gypseum was only 6 mm. UV-vis spectral analysis revealed the compound belonged to nucleoside antibiotics. Conclusion: It is evident that the mangrove actinomycetes are potential for preparing biomedicines for human welfare.

scholarly journals In VivoEvaluation ofGalla chinensisSolution in the Topical Treatment of Dermatophytosis

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Kai Sun ◽  
Xu Song ◽  
RenYong Jia ◽  
Zhongqiong Yin ◽  
Yuanfeng Zou ◽  
...  
Keyword(s):  
Trichophyton Mentagrophytes ◽  
Clinical Score ◽  
Skin Lesions ◽  
Positive Control ◽  
Microsporum Canis ◽  
Microsporum Gypseum ◽  
Pharmacological Effects ◽  
Mycological Cure ◽  
Candidate Drug ◽  
Cure Rates

Aim. Dermatophytosis is one of the main fungal diseases in humans and animals all over the world.Galla chinensis, a traditional medicine, has various pharmacological effects. The goal of this study was to evaluate the treatment effect ofGalla chinensissolution (GCS) on dermatophytosis-infected dogs (Microsporum canis,Microsporum gypseum, andTrichophyton mentagrophytes, resp.).Methods. The treatment effects of GCS were evaluated by mycological cure rates and clinical score comprised of three indices, including inflammation, hair loss, and lesion scale.Results. The results showed that, in the three models of dermatophytosis, GCS significantly (P<0.05) improved skin lesions and fungal eradication. GCS (10% and 5%) had higher efficacy compared to the positive control (Tujingpi Tincture). The fungal eradication efficacy exceeds 85% after treatment with GCS (10%, 5%, and 2.5%) on day 14.Conclusion. The GCS has antidermatophytosis effect in dogs, which may be a candidate drug for the treatment of dermatophytosis.

Saprophytic occurrence of Trichophyton mentagrophytes and Microsporum gypseum in the coats of healthy laboratory animals

1975 ◽  
Vol 55 (1) ◽  
pp. 13-15 ◽  
Author(s):  
E. Feuerman ◽  
I. Alteras ◽  
E. H�nig ◽  
N. Lehrer
Keyword(s):  
Trichophyton Mentagrophytes ◽  
Laboratory Animals ◽  
Microsporum Gypseum

scholarly journals Incidence of Keratinophilic Fungi from Selected Soils of Vidarbha Region of Maharashtra State, India

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sunil Kumar Deshmukh ◽  
Shilpa Amit Verekar
Keyword(s):  
Trichophyton Mentagrophytes ◽  
Keratinophilic Fungi ◽  
Microsporum Gypseum ◽  
Its2 Rdna ◽  
Blast Search ◽  
Chrysosporium Tropicum

One hundred and fifty samples were collected from eleven districts of Vidarbha region of Maharashtra state and screened for the presence of keratinophilic fungi using hair baiting technique for isolation. Seventy-one isolates were recovered and identified. The cultures were identified using macro- and micromorphological features. Their identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/Genbank data and compared with deposited sequences for identification purpose. Thirteen species of eight genera were isolated, namely, Auxarthron conjugatum (2.00%), Chrysosporium indicum (14.00%), Chrysosporium evolceanui (2.66%), Chrysosporium tropicum (4.66%), Chrysosporium zonatum (1.33%), Chrysosporium state of Ctenomyces serratus (3.33%), Gymnascella dankaliensis (1.33%), Gymnascella hyalinospora (0.66%), Gymnoascoideus petalosporus (0.66%), Microsporum gypseum complex (9.33%), Trichophyton mentagrophytes (2.00%), T. terrestre (3.33%), and Uncinocarpus queenslandicus (2.00%). This study indicates that the soils of Vidarbha region of Maharashtra may be significant reservoirs of certain keratinophilic fungi.

scholarly journals Prevalence of keratinophilic fungi in public park soils of Mumbai, India

2012 ◽  
Vol 3 (1) ◽  
pp. 6 ◽  
Author(s):  
Sunil Kumar Deshmukh ◽  
Shilpa Amit Verekar
Keyword(s):  
Human Hair ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Soil Samples ◽  
Keratinophilic Fungi ◽  
Shake Flask Culture ◽  
Microsporum Gypseum ◽  
Public Parks ◽  
Liquid Media ◽  
Public Park

The parks of Mumbai are frequently visited by local residents every morning and evening. However, there are no reports on the occurrence of keratinophilic fungi in these areas. The purpose of this research was to study the occurrence of keratinophilic fungi in the public parks of Mumbai. One hundred soil samples were collected from five public parks: Kamla Nehru Park, Powai Garden, CD Deshmukh Garden, Five Gardens and Chota Kashmir. Keratinophilic fungi were isolated by the hair baiting technique using human hair as keratin bait. The cultures were identified using macroand micro-morphological features. Identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/Genbank data and compared with deposited sequences. The ability of these fungi to use human hair was also evaluated by release of protein in liquid media. A total of 75 strains of keratinophilic fungi were recovered from 100 (75.0%) soil samples. The isolated fungi were composed of eleven species of eight genera: <em>Arthrographis kalrae</em>, A<em>uxarthron conjugatum</em>, <em>Chrysosporium indicum</em>, <em>C. queenslandicum</em>, <em>C. zonatum, Gymnascella dankaliensis</em>, <em>G. hyalinospora</em>,<em> Microsporum gypseum</em> (15.0%), <em>Myriodontium keratinophilum</em>, <em>Trichophyton mentagrophytes</em> and <em>Uncinocarpus reesii</em>. These fungi can release 148.8-307.6 μg/mL protein in liquid media when grown on human hair in shake flask culture and also decompose 16.2-38.6% of human hair after four weeks of incubation. Our study indicates that keratinophilic fungi are to be found in the soils of various public parks in Mumbai and that human hair can be a source of pathogenic fungi.

scholarly journals Antimycotic Activity of Leaf Extracts of Medicinal Plants Against Dermatophytes

2020 ◽  
Vol 1 (1) ◽  
pp. 33-40
Author(s):  
Renu Jangid ◽  
Tahira vbegum
Keyword(s):  
Medicinal Plants ◽  
Diethyl Ether ◽  
Trichophyton Mentagrophytes ◽  
Ziziphus Mauritiana ◽  
Moderate Activity ◽  
Microsporum Gypseum ◽  
Leaf Extracts ◽  
Plant Materials ◽  
Antimycotic Activity

Plant products have been used as medicines against fungal infectious diseases. In this research antimycotic activity of the leaf extracts of five medicinal plants (Nerium indicum, Catheranthus roseus, Lantana camera, Ziziphus mauritiana) were tested against three dermatophytes (Trichophyton mentagrophytes, Trichophyton rubrum and Microsporum. gypseum). Development of more effective and less toxic antimycotic agents is required for the treatment of dermatophytosis. The plant materials were extracted with methanol, ethanol and diethyl ether solvent to investigate their antimycotic activities in Vitro. Ethanol and methanol extracts of all selected medicinal plants were showed the positive activity against all tested dermatophytes. Diethyl ether extract was showed lowest activity against T. mentagrophytes and T. rubrum and showed moderate activity against M. gypseum. The three dermatophytes differed with regard to their susceptibility to plant extracts.

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