scholarly journals DETERMINATION OF TOTAL PHENOLIC CONTENT FROM PLANT EXTRACTS USED IN COSMETIC PURPOSE

2021 ◽  
Vol 21 (1) ◽  
pp. 247-260
Author(s):  
SIMONA DOBRINAS ◽  
ALINA SOCEANU
Keyword(s):  
Measurement Uncertainty ◽  
Gallic Acid ◽  
Acid Concentration ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Limit Of Detection ◽  
Total Phenolic ◽  
Spectrometric Method ◽  
Calibration Equation

Spectrometric analysis belongs to a group of measurement methods used for their simplicity and large selectivity to solve various problems of analytes. The total phenolic content of different plant extracts used for cosmetic purpose was determined by a slightly modified version of traditionally Folin-Ciocalteau method. The objective of this work was the validation of UV-Vis spectrometric method, investigation of uncertainty sources when measuring gallic acid concentration and evaluation of the calibration equation effect on measurement uncertainty of UV– Vis spectrophotometer. Validation was performed by studying analytical curve linearity (R2=0.9995) and range (37.5 – 225 mg L-1), estimated limit of detection (LOD, 0.11mg L-1) and limit of quantification (LOQ, 0.37 mg L-1), precision (%RSD, 0.14 – 1.34), recoveries (83-110%) and stability (%RSD, 0.8 – 2.83). To obtain more representative values for precision, recoveries and stability simultaneous replicates at different times, on different matrices including plant (marigold, chamomile and lavender) were performed during the study period. The validated method was successfully applied to determine TPC in marigold extracts. For chamomile and lavender extracts, the spectrometric method presented only acceptable precision, among all the performance parameters studied. The sources of the gallic acid concentration measurement uncertainty include purity, volume of flasks, mass and the calibration equation. The results indicated that the uncertainty components from purity were the smallest. An important source of the uncertainty was the mass.The volumes of the volumetric flasks had only modest effect on the uncertainty. The contribution of calibration equation is the greatest from all sources.

scholarly journals Polyphenols in Coreopsis tinctoria Nutt. fruits and the plant extracts antioxidant capacity evaluation

2014 ◽  
Vol 12 (8) ◽  
pp. 858-867 ◽  
Author(s):  
Christina Zălaru ◽  
Claudia Crişan ◽  
Ioan Călinescu ◽  
Zenovia Moldovan ◽  
Isabela Ţârcomnicu ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Optimum Operating ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Coreopsis Tinctoria

AbstractThe aim of this research was to investigate the chemical composition of Coreopsis tinctoria Nutt. fruits extract, to highlight the potential of ultrasound assisted extraction in the fast preparation of extracts rich in polyphenols using different solvents (55%, 78% and 96% hydrous ethanol) and to evaluate the antioxidant potential of formulated extracts. LC-MS/MS was used to characterize the ethanolic extract from Coreopsis tinctoria Nutt. dried fruits. The extract contains different flavonoids (marein, flavanomarein, quercetagetin-7-O-glucoside, okanin aurone, leptosidin, luteolin, apigenin) and phenolic acids (chlorogenic acid, caffeic acid). Several parameters that could affect extraction efficiency were evaluated. Finally, this study focused on determination of plant extracts total phenolic content and their antioxidant capacity. The experimental results allowed the selection of the optimum operating parameters leading to the highest total polyphenolic content, expressed as gallic acid equivalents, and avoiding the degradation of phenolic compounds (ethanol 55%; extraction temperature 323.15 K, extraction time 30 min, liquid/solid ratio 20/1). A good relationship between total phenolic content and antioxidant capacity was obtained.

The Effect of Heating Temperature on Total Phenolic Content, Antioxidant Activity, and Phenolic Compounds of Plum and Mahaleb Fruits

2019 ◽  
Vol 15 (11-12) ◽  
Author(s):  
Kashif Ghafoor ◽  
Isam A. Mohamed Ahmed ◽  
Süleyman Doğu ◽  
Nurhan Uslu ◽  
Gbemisola J. Fadimu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Heating Temperature ◽  
Dry Weight ◽  
Total Phenolic ◽  
Dihydroxybenzoic Acid ◽  
Different Temperatures

AbstractThe effect of heating at different temperatures (60, 80, 90, 110, and 130 °C) on the total phenolic content, antioxidant activity, and phenolic compounds present in plum and mahaleb fruits was investigated. The antioxidant activity values and total phenolic contents of fresh plum (93.82% measured by DPPH method, 787.79 mg gallic acid equivalent (GAE)/100 g dry weight determined by Folin method) and mahaleb fruits (81.80%, 634.47 mg GAE/100 g dry weight) were higher than plum and mahaleb fruits dried at different temperatures (p < 0.05). Generally, the heating process caused a reduction in both total phenolic content and antioxidant activity for plum and mahaleb. While (+)-catechin (92.62 mg/kg), 1,2-dihydroxybenzene (132.15 mg/kg), gallic acid (107.01 mg/kg), and 3,4-dihydroxybenzoic acid (74.59 mg/kg) are the key phenolic compounds in fresh plum, (+)-catechin, 3,4-dihydroxybenzoic acid, 1,2-dihydroxybenzene, and syringic and caffeic acids were the major phenolic compounds of mahaleb fruits. The polyphenol content of fruits and the class of phenolics present are significantly affected by heating temperature.

Novel determination of the total phenolic content in crude plant extracts by the use of 1H NMR of the –OH spectral region

2011 ◽  
Vol 688 (1) ◽  
pp. 54-60 ◽  
Author(s):  
A.A. Nerantzaki ◽  
C.G. Tsiafoulis ◽  
P. Charisiadis ◽  
V.G. Kontogianni ◽  
I.P. Gerothanassis
Keyword(s):  
1H Nmr ◽  
Plant Extracts ◽  
Spectral Region ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic

Optimization of Drying and Extraction Process of Pereskia bleo Leaves in Determining Antioxidant Properties Utilizing Pareto ANOVA

2014 ◽  
Vol 548-549 ◽  
pp. 96-100
Author(s):  
Shareena Fairuz Abdul Manaf ◽  
Nur Shahidah Ab Aziz ◽  
Syawal Abdullah ◽  
Rafeqah Raslan ◽  
Fazlena Hamzah ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Herbal Medicines ◽  
Extraction Process ◽  
Total Phenolic ◽  
Drying Time ◽  
Standard Curve ◽  
Three Factor

Pereskia Bleois commonly used among the local traditional medicine practitioners to prevent or treat cancer by consuming the leaves either raw or taken as a concoction brewed from fresh leaves. However, more research, regulation and standardization are required before herbal medicines can be recommended as effective and safe therapies. The objectives of this study are to obtain the optimum drying parameter and to identify the concentrations of phenolic content expressed as gallic acid equivalents (GAEs) inPereskia bleoleaves. Pareto ANOVA method was used to study the optimum condition and the total phenolic content via F-Test using three samples for variance and three-factor with replication. Taguchi ANOVA was performed using SN ratio with L9 orthogonal array to accommodate the three factor level. The drying time were tested for 3 hours, 4 hours and 5 hours. The result shows that drying time is the significant factor in drying process and followed by drying temperature and solvent volume used. The drying experiments were conducted at operating temperature of 35 °C, 40 °C and 45 °C. While the extraction were conducted at different volume using methanol of 150 ml, 180 ml and 200 ml. The standard curve of total phenolic content was based on the gallic acid compound. The total phenolic content in C2 was at the most suitable for being used as antioxidant for consumption in human body with drying time of 5 hours at 45 °C by using 180 ml of methanol for extraction ofP.bleoleaves.

scholarly journals Investigation on Green Synthesis, Biocompatibility, and Antibacterial Activity of Silver Nanoparticles Prepared Using Cistus incanus

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 5028
Author(s):  
Wioletta Florkiewicz ◽  
Klaudia Pluta ◽  
Dagmara Malina ◽  
Karolina Rudnicka ◽  
Anna Żywicka ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Dry Weight ◽  
Total Phenolic ◽  
Composition Analysis ◽  
Cistus Incanus

This paper describes the plant-mediated preparation of silver nanoparticles with aqueous extract and infusion of Cistus incanus leaves. To evaluate aqueous extract and infusion antioxidant capacity and total phenolic content the DPPH and Folin–Ciocalteau methods were utilized. The antioxidant capacity and total phenolic content of extract and infusion were equal to 85.97 ± 6.54 mg gallic acid equivalents per gram of dry weight.; 10.76 ± 0.59 mg/mL and 12.65 ± 1.04 mg gallic acid equivalents per gram of dry weight.; 3.10 ± 0.14 mg/mL, respectively. The formed nanoparticles displayed the characteristic absorption band in the 380–450 nm wavelength range. The average size of particles was in the 68.8–71.2 nm range. Morphology and phase composition analysis revealed the formation of spherical nanoparticles with a face-centred cubic structure. Immune compatibility tests of nanoparticles and plant extracts showed no activation of the THP1-XBlue™ monocyte. Cytotoxicity tests performed with L929 mice fibroblasts showed that nanoparticles should be utilized at a concentration of 16 ppm. The minimum inhibitory concentrations determined with the microdilution method for nanoparticles prepared with plant infusion for S. aureus and S. epidermidis were 2 ppm and 16 ppm, respectively.

Phenolic Composition and Antioxidant Activity of Leaves of Strawberry Tree (Arbutus unedo L.) Populations from Morocco

2021 ◽  
Author(s):  
Z. Ait lhaj ◽  
K. Taghzouti ◽  
A. Bouyahya ◽  
G. Diria ◽  
K. Bakhy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Scavenging Effect ◽  
Promising Source

This study was aimed at determining the phenolic composition and the antioxidant activity of leaves of Arbutus unedo populations belonging to seven Moroccan locations. Antioxidant activity is evaluated using two methods: scavenging effect on DPPH and scavenging effect on ABTS radicals. The Arbutus unedo leaf extracts displayed average values of 61.95 mg GAE, 3.60 mg QE, and 0.15 mg CE per g of dry weight (DW) in polyphenols, flavonoids, and tannins contents, respectively. All extracts exhibited a strong antioxidant effect on both assays, presenting mean IC50 values of 4.22 in DPPH assay and 4.86 μg/ml in ABTS assay. Significant variability (P < 0.0001) was showed among surveyed populations; samples from the Chefchaouen location (CH) was revealed the highest total phenolic content (107.67 mg GAE/g DW) and the strongest antioxidant activity (IC50 values of 0.80 and 1.56 μg/ml, in DPPH and ABTS tests, respectively). The phenolic profile achieved by HPLC-DAD-ESI/MS was dominated by gallic acid, Gallic acid glucose derivative, and Galloylquinic acid derivative. A significant negative correlation (P < 0.05) was observed between the total phenolic content and the antioxidant activity. These results provide that Arbutus unedo leaves should be considered as a promising source of natural antioxidants, and as ingredients in complimentary food and nutraceutical products.

scholarly journals Chemical composition and antioxidant activity of geopropolis produced by Melipona fasciculata (Meliponinae) in flooded fields and cerrado areas of Maranhão State, northeastern Brazil

2016 ◽  
Vol 46 (3) ◽  
pp. 315-322 ◽  
Author(s):  
Marisa Cristina Aranha BATISTA ◽  
Bruno Vinicius de Barros ABREU ◽  
Richard Pereira DUTRA ◽  
Mayara Soares CUNHA ◽  
Flavia Maria Mendonça do AMARAL ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Gallic Acid ◽  
Phenolic Acids ◽  
Ellagic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Northeastern Brazil ◽  
Total Phenolic ◽  
Antioxidant Power

ABSTRACT Geopropolis, a mixture of plant resin, wax, soil and salivary secretion, is produced by the stingless bee Melipona fasciculata. This aim of this study was to investigate the chemical composition and antioxidant activity of geopropolis collected from beehives in two phytogeographical regions, flooded fields and cerrado, in the municipalities of Palmeirândia and Fernando Falcão, Maranhão State, northeastern Brazil. The geopropolis compounds were identified by gas chromatography-mass spectrometry (GC/MS). Additionally, total phenolic content was determined with the Folin−Ciocalteu reagent and antioxidant activity was evaluated in vitro by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The four geopropolis samples varied in terms of total phenolic content and antioxidant activity and the highest values were observed for geopropolis from Fernando Falcão. Triterpenes such as cycloartane, ursane and oleanane and phenolic acids (protocatechuic acid and gallic acid) were identified in the geopropolis from Palmeirândia, while the phenolic acids, gallic and ellagic acid were the main compounds in geopropolis from Fernando Falcão. The antioxidant property of geopropolis is due to its high total phenolic content and predominance of gallic acid and ellagic acid. The results showed that the plant resources in two phytogeographical regions of Maranhão used by bees for the production of geopropolis contribute to the chemical composition and bioactivity of this product.

scholarly journals PENETAPAN KADAR FENOLIK TOTAL FRAKSI POLR DAN NONPOLAR DAUN RAMBAI LAUT (Sonneratia caseolaris L.) DENGAN METODE SPEKTROFOTOMETRI UV-Vis

2019 ◽  
Vol 1 (2) ◽  
pp. 140-147
Author(s):  
Siti Jubaidah ◽  
Reksi Sundu ◽  
Nur Sabriningsih
Keyword(s):  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Polar Fraction ◽  
Total Phenolic ◽  
Mangrove Forests ◽  
Sonneratia Caseolaris ◽  
Nonpolar Fraction

Rambai laut (Soneratia caseolaris L.) is a type of tree that grows in riverbank swamps or mangrove forests which contains one of the secondary metabolites in the form of phenolic compounds. The purpose of this study was to determine the total phenolic content of polar and nonpolar fractions of sea rambai leaves using UV-Vis spectrophotometry method. Research conducted is non-experimental. The stages of the study included the determination of plants, sampling, making samples, making extracts, determining the water content of extracts, fractionation using polar and nonpolar fractions, phytochemical screening test of ethanol extract and fractionation results and determination of total phenolic levels by UV-Vis spectrophotometry. The results showed that rambai laut leaf in polar fraction had a total phenolic content of 213.49 ± 1.2227 mg GAE / g, which means that in each gram the polar fraction is equivalent to 213.49 mg of gallic acid, whereas in the nonpolar fraction of rambai laut leaves is obtained 55.79 ± 1.0809 mg GAE / g which means that in each gram the nonpolar fraction is equivalent to 55.79 mg of gallic acid. Total phenolic content is greater in the polar fraction.

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