scholarly journals Smart Production of Lipids as Bio-Fuel in Spirulina Platensis (=Arthrospira Fusiformis), and Bio-Oxygen and Bio-Electricity in Media Cultured in Supernatant of Digested Poultry Waste

2021 ◽  
Vol 04 (04) ◽  
Author(s):  
M.A. Satter ◽  
Keyword(s):  
Chlorophyll A ◽  
Spirulina Platensis ◽  
Culture Media ◽  
Total Biomass ◽  
Poultry Waste ◽  
Cell Weight ◽  
A Cell ◽  
The Media ◽  
Specific Growth Rates ◽  
Chlorophyll A Content

An experiment was conducted to evaluate growth performances and production of bio-fuel of Spirulina platensis (Gomont), and bio-oxygen and bio-electricity of culture media in supernatant of three different amount of digested poultry waste (DPW), and Kosaric medium (KM) as control. Three different amounts (concentrations) such as 2.0, 4.0 and 6.0 g/L poultry waste were allowed to digest under aeration. After 17 days, 700 ml grayish coloured supernatant was taken with addition of 9.0 g/L NaHCO3 and 0.50 ml/l micronutrient in 2.0 L conical flask with three replications and then autoclaved. Spirulina was inoculated to grow in these three treatments including KM (Control) after 72 hours of autoclave and then allowed to grow for a period of 14 days. This duration was estimated through repeated growth trials. The cell weight of spirulina was attained a maximum of 12.58 ± 1.25 mg/L (dry wt. basis) in KM followed by 11.46 ± 1.03, 9.16 ± 0.84 and 8.13 ± 0.73 mg/L in supernatant of 4.0, 2.0 and 6.0 g/L DPW, respectively on the 10th day of culture. Similar trend was also observed in the cases of optical density of the media contained spirulina, chlorophyll a content (mg/L), cell weight, total biomass (mg/l) and specific growth rates on the basis of cell weight and chlorophyll a. Cell weight of spirulina grown in these media had highly significant (P < 0.01) correlation with the chlorophyll a content (r = 0.993) of spirulina. Crude protein of spirulina grown in supernatant of DPW was lower than that of spirulina cultured in KM. Crude lipids as bio-fuel of spirulina cultured in supernatant of 4.0 g/L DPW was almost three times higher than that of spirulina grown in KM which may be due to high phospholipids bioaccumulation. Phosphate-P was decreased in media of DPW due to its use for high lipids biosynthesis as phospholipids. Bio-oxygen was produced higher ranged from 2.1 ± 11 mg/L on initial day to 10.20 ± 0.54 mg/L on 10th day grown in supernatant of 4.0 g/L DPW media. Bio-electricity as green electricity ranged from 135 ± 4 on initial day to 284 ± 7 mV on 10th day when spirulina cultured in supernatant of 4.0 g/L DPW which was higher than grown in other media. pH followed the similar trend like bio-oxygen and bio-electricity. It was found that the production of bio-oxygen, bio-fuel and bio-electricity had direct relation with pH in culture media of spirulina. Therefore, mass production of Spirulina platensis might be done in supernatant of 4.0 g/L digested poultry waste to get high total lipids as bio-fuel, bio-electricity and bio-oxygen.

scholarly journals Smart Culture of Spirulina Using Supernatant of Digested Rotten Tomato (Solanum Lycopersicum) to Produce Protein, Bio-Fuel and Bio-Electricity

2021 ◽  
Vol 04 (03) ◽  
Author(s):  
M. Ahsan B. Habib ◽  
Keyword(s):  
Electric Conductivity ◽  
Solanum Lycopersicum ◽  
Chlorophyll A ◽  
Spirulina Platensis ◽  
Culture Media ◽  
Chemical Properties ◽  
Electricity Production ◽  
Total Biomass ◽  
Cell Weight ◽  
Growth Performances

An experiment was conducted for the production of protein, bio-fuel and bio-electricity from the culture system of Spirulina platensis (Gomont) in supernatant of three different amount of digested rotten tomato (Solanum lycopersicum), and Kosaric Medium (KM) as control. Three different concentrations such as 25, 50 and 75% rotten tomato were allowed to digest under aeration. After 17 days, the colorless supernatant was screened and taken in 1.0 L conical flask with three replications. Then, Spirulina platensis was inoculated to grow in these three media (treatments) with the addition of 9.0 g/L NaHCO3 and micronutrients, and also in KM as control for a period of 14 days. The cell weight, optical density, chlorophyll a and total biomass of spirulina was attained to the maximum values when grew in KM on the 10th day of culture followed by supernatant of 50% digested rotten tomato (DRT) than in 25 and 75% DRT culture. The chemical properties of the culture media such as pH, salinity, dissolved bio-oxygen, electric conductivity and bio-electricity were increased from first day up to 12th day of experiment. Total biomass of spirulina grown in these media had highly significant (P < 0.01) correlation with cell weight (r = 0.825) and chlorophyll a (r = 0.866) of spirulina. The results showed that the growth performances of S. platensis grown in supernatant of 50% DRT was significantly (P < 0.01) higher than that of spirulina grown in supernatant of 25 and 75% DRT. The percentage of crude protein (55.10 ± 0.45 to 59.90 ± 0.33%) of spirulina grown in supernatant of DRT was little bit higher than that of spirulina cultured in KM (58.40 ± 0.38%). But bio-fuel in terms of crude lipids (16.50 ± 0.31%) of spirulina cultured in supernatant of 50% DRT was almost two and half times higher than that of spirulina grown in KM (crude lipids, 6.30 ± 0.21%). Bio-electricity (300 ±10.20 mV) produced in culture of spirulina in supernatant of 50% DRT was higher than that recorded in KM (240 ±10.20 mV) followed by 75% DRT and other media. Bio-electricity had directly and strongly significant (p < 0.001) correlation with pH (r = 0.812), dissolved bio-oxygen (r = 0.832), salinity (r = 0.788) and electric conductivity (r = 0.856). Therefore, this procedure will produce huge amount of electricity in the world and will make a revolution in this field of bio-electricity production. Whole world will be benefited from the output (results) of this experiment.

scholarly journals Growth Performance and Nutritional Analysis of Spirulina platensis in Different Concentrations of Papaya Skin Powder Media

1970 ◽  
Vol 46 (3) ◽  
pp. 333-338 ◽  
Author(s):  
MA Toyub ◽  
MZ Uddin ◽  
MI Miah ◽  
MAB Habib
Keyword(s):  
Growth Performance ◽  
Chlorophyll A ◽  
Spirulina Platensis ◽  
Specific Growth ◽  
Cell Weight ◽  
Skin Cell ◽  
Physico Chemical ◽  
Nutritional Analysis ◽  
Chemical Factors ◽  
Chlorophyll A Content

The growth performance of Spirulina platensis was studied in four different concentrations viz. 0.3, 0.4, 0.5 and 0.6 g/l of papaya (Carica papaya) skin powder media (PSPM) and in control, Kosaric Medium (KM) in triplicate. The initial cell weight of S. platensis was 6.0 mg/l which attained a maximum weight of 913.13 mg/l on the 10th day of culture in the control KM followed by 699.07, 612.13, 538.99 and 377.48 mg/l that grown in 0.40, 0.30, 0.50 and 0.60 g/l of PSPM respectively. Similar trend was observed in the case of chlorophyll a content ranging 3.57 to 8.15 mg/l. The specific growth rate (SGR, μ/day) of S. platensis on the basis of cell weight and chlorophyll a content was recorded in the ranges of 0.41 to 0.50 and 0.41 to 0.49, respectively, for all the treatments. The SGR on the basis of cell weight and chlorophyll a was recorded significantly (p<0.05) higher in KM among all the treatments. On the other hand among the four different concentrations of PSPM, 0.40 g/l showed significantly (p<0.05) higher SGR than others. The pH and other physico-chemical factors were within suitable range of algae culture. The cultured microalga was nutritionally rich. Keywords: Spirulina; Papaya skin; Cell weight ; Chlorophyll a DOI: http://dx.doi.org/10.3329/bjsir.v46i3.9039 BJSIR 2011; 46(3): 333-338

Growth Performance and Nutritional Value of Chlorella ellipsoidea in Fertilizer Factory Effluent Media

2007 ◽  
Vol 20 (1) ◽  
Author(s):  
M. A. TOYUB ◽  
S.R. AHMED ◽  
M. I. MIAH ◽  
M. A. B. HABIB
Keyword(s):  
Growth Performance ◽  
Chlorophyll A ◽  
Nutritional Value ◽  
Specific Growth ◽  
Basal Medium ◽  
Cell Number ◽  
Total Biomass ◽  
Chlorella Ellipsoidea ◽  
Green Alga Chlorella ◽  
Chlorophyll A Content

The growth performance of green alga, Chlorella ellipsoidea was studied in a laboratory in different concentrations of fertilizer factory effluent media (FFEM). Five different concentrations viz. 40, 45, 50, 55 and 60% of FFEM and bold basal medium (BBM) (control) were used with three replications for a period of three months. Each trial was done for a period of 16 days. The initial cell density of C. ellipsoidea was 2.5×105 cells.ml-1 which attained a maximum density of 198.49x105 cells.ml-1 in BBM followed by 182.07, 157.41, 142.34, 137.57 and 121.35 (x105 cells. ml-1) in 50, 55, 45, 60 and 40 % FFEM, respectively on the 10th day of culture. A similar trend was observed in the case of chlorophyll a content and the range was 5.85 to 9.39 mg.l-1. The specific growth rate (SGR, μ.day-1) on the basis of cell number and chlorophyll a were found at 0.40 to 0.44 and 0.41 to 0.45, respectively and was significantly (p<0.05) higher in BBM. The total biomass was found at 629.13 and 541.36 mg.l-1 in BBM and 50% FFEM, respectively. The overall growth performance of C. ellipsoidea was significantly (p<0.05) higher in 50% FFEM than in other concentrations of FFEM. The cultured microalga was found nutritionally rich.

Replacement of Sodium Bicarbonate and Micronutrients in Kosaric Medium With Banana Leaf Ash Extract for Culture of Spirulina Platensis

2020 ◽  
Vol 23 (1) ◽  
pp. 37-47
Author(s):  
S Khatun ◽  
MA Hossain ◽  
T Akter ◽  
MR Banu ◽  
AQMR Kawser
Keyword(s):  
Sodium Bicarbonate ◽  
Spirulina Platensis ◽  
Culture Media ◽  
Dry Weight ◽  
Chemical Parameters ◽  
Nutritional Values ◽  
Maximum Cell ◽  
Physical And Chemical ◽  
Banana Leaf ◽  
Chlorophyll A Content

Spirulina platensis is one of the most promising microalgae for culture due to its high nutritional values. The main constraint to its production is the high cost of culture media. Therefore, an experiment was conducted to evaluate the growth performance of S. platensis with the aim of replacing sodium bicarbonate (NaHCO3) and mineral nutrients in Kosaric medium (KM) with banana leaf ash extract. KM was used as control medium in treatment T1. In treatments T2 to T5, 50% of NaHCO3 in KM was replaced with banana leaf ash extract (BLAE). In addition, micronutrients in KM were reduced to 75%, 50% and 25% in treatments T3 to T5, respectively. The values of different physical and chemical parameters (temperature, pH, dissolved oxygen and light intensity) of the culture media were within the suitable range for S. platensis culture. Optical density in treatments T2 and T3 were similar to that was in treatment T1. Maximum cell dry weight (0.65 gL-1) was observed in treatment T1 and it was similar to the treatments T2 and T3 where 50% of NaHCO3 in KM was replaced with banana leaf ash extract with 100% and 75% micronutrients supply, respectively. The similar trend was found in chlorophyll a content. It was evident that 50% of NaHCO3 in KM can be replaced with BLAE for the culture of S. platensis, in addition micronutrients in KM can be reduced to 75%. Ann. Bangladesh Agric. (2019) 23(1) : 37-47

scholarly journals Modeling Of Chlorophyll a Content in Microalgae Cultures

2020 ◽  
Vol 15 (2) ◽  
pp. 158-171 ◽  
Author(s):  
A.S. Lelekov ◽  
R.P. Trenkenshu
Keyword(s):  
Chlorophyll A ◽  
Arthrospira Platensis ◽  
Basic Model ◽  
Cell Structures ◽  
Kinetic Coefficients ◽  
Microalgae Biomass ◽  
Species Specific ◽  
Specific Growth Rates ◽  
Chlorophyll A Content ◽  
Good Agreement

The work focuses on mathematical modeling of light influence mechanisms on chlorophyll a content in microalgae biomass. The well-known qualitative models are based on concepts of synthesis and photodestructive oxidation of chlorophyll a, however the later for some microalgae species seems doubtful. We proposed an alternative approach to modeling the light-dependent chlorophyll a content in microalgae biomass. The basic model is based on generally accepted two-stage photoautotrophic growth of microalgae. At the first stage, during photosynthesis a reserve part of biomass is formed, from which the biosynthesis of cell structures occurs at the second stage. Three partial solutions of the basic system of equations describing the dependence of chlorophyll a content on the external light intensity are considered for various limiting conditions. Due to the equality of specific growth rates of formation of reserve and structural forms of biomass, the equations obtained can be used only for turbidostat cultures. Verification of the obtained equations for Arthrospira platensis allows us to estimate kinetic coefficients, the values of which are generally in good agreement with theoretically calculated ones. For approximate calculations, a simple equation is proposed that shows a good agreement with experimental data for Tetraselmis viridis (R2 = 0.98), Dunaliella tertiolecta (R2 = 0.92) and describes the results for Sceletonema costatum and Chlorella vulgaris (R2 = 0.8) quite well. Chlorophyll a refers to structural forms of biomass. The proportion of chlorophyll a in the structural biomass is about 2.5–3.5 %, it is a species-specific parameter.

Growth performance analysis of Spirulina platensis production by substituting K2SO4-K of kosaric medium with MOP-K

2019 ◽  
Vol 48 (3) ◽  
pp. 529-535
Author(s):  
Taslima Akter ◽  
Md Imdadul Haque ◽  
Mousumi Das ◽  
Md Amzad Hossain
Keyword(s):  
Chlorophyll A ◽  
Spirulina Platensis ◽  
Low Cost ◽  
Dry Weight ◽  
Control Treatment ◽  
Optical Cell ◽  
Cell Biomass ◽  
Significant Difference ◽  
Chlorophyll A Content ◽  
Muriate Of Potash

Five media were formulated with 0% (control, T1), 25% (T2), 50% (T3), 75% (T4) and 100% (T5) inclusion of low-cost muriate of potash (MOP)-potassium (K) replacing high-cost reagent K2SO4-K and Spirulina platensis was cultured for 18 days. Cell dry weight, optical cell density and chlorophyll-a content of S. platensis cultured in five treatments were registered at every three-day interval and economic performance was calculated to observe the effect of K2SO4-K replacement with MOP-K. The cell biomass production and chlorophyll-a content of S. platensis cultured in 25 and 50% use of MOP-K instead of K2SO4-K (T2 and T3) did not represent any significant difference with the control treatment of 100% K2SO4-K (T1). However, further addition of MOP-K in T4 and T5 significantly reduced the cell growth and pigment content of S. platensis. In addition, a significant reduction of production cost was calculated as more percentage of K2SO4- K was replaced with MOP-K.

scholarly journals Growth Performance And Nutritional Value Of Scenedesmus Obliquus Cultured In Different Concentrations Of Sweetmeat Factory Waste Media

1970 ◽  
Vol 37 (1) ◽  
pp. 86-93 ◽  
Author(s):  
MA Toyub ◽  
MI Miah ◽  
MAB Habib ◽  
MM Rahman
Keyword(s):  
Growth Performance ◽  
Culture Media ◽  
Scenedesmus Obliquus ◽  
Initial Density ◽  
Basal Medium ◽  
Total Biomass ◽  
Microalgae Culture ◽  
Physico Chemical ◽  
Ph Range ◽  
Chlorophyll A Content

The growth performance of Scenedesmus obliquus was studied in the laboratory in different concentration of sweetmeat factory waste media (SFWM). Four different concentrations viz. 1.5, 2.0, 2.5 and 3.0% of SFWM and Bold basal medium (BBM) as control was used with three replications for a period of three months. Each trial was done for a period of 14 days in the laboratory. The initial density of Scenedesmus obliquus was 2.1×105 cell/ml which attained a maximum density of 136.30×105 cell/ml in BBM followed by 97.05, 83.21, 65.19 and 51.21 (×105 cell/ml) in 2.5, 0.3, 2.0 and 1.5 % SFWM respectively on the 10th day of culture. Highest chlorophyll a content was also observed on the 10th day of culture with value of 6.14 and 5.52 mg/l in BBM and SFWM, respectively. The specific growth rate (SGR, m/day) and total biomass were significantly (P<0.05) higher in BBM than all other treatments. The overall growth performance of the alga Scenedesmus obliquus was significantly (P<0.05) higher in 2.5% SFWM than other concentrations of SFWM. The cultured microalgae were found nutritionally rich. During the whole culture period the pH range was 7.03 to 8.05. The other physico-chemical parameters of the culture media were found within the suitable range of the microalgae culture. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9874 BJAS 2008; 37(1): 86-93

Estimation of chlorophyll content by absorption spectra of native Spirulina platensis cells

2020 ◽  
pp. 25-33
Author(s):  
Leonid E. Paramonov
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Absorption Spectra ◽  
Spirulina Platensis ◽  
Intracellular Concentration ◽  
Absorption Coefficients

A method for retrieving the absorption coefficients of Spirulina platensis pigments using absorption spectra of native cells and excluding the use of extracts is considered. Estimates of the intracellular concentration of chlorophyll a, С-phycoerythrin, С- phycocyanin and allophycocyanin in native cells are discussed.

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