Replacement of Sodium Bicarbonate and Micronutrients in Kosaric Medium With Banana Leaf Ash Extract for Culture of Spirulina Platensis

2020 ◽  
Vol 23 (1) ◽  
pp. 37-47
Author(s):  
S Khatun ◽  
MA Hossain ◽  
T Akter ◽  
MR Banu ◽  
AQMR Kawser
Keyword(s):  
Sodium Bicarbonate ◽  
Spirulina Platensis ◽  
Culture Media ◽  
Dry Weight ◽  
Chemical Parameters ◽  
Nutritional Values ◽  
Maximum Cell ◽  
Physical And Chemical ◽  
Banana Leaf ◽  
Chlorophyll A Content

Spirulina platensis is one of the most promising microalgae for culture due to its high nutritional values. The main constraint to its production is the high cost of culture media. Therefore, an experiment was conducted to evaluate the growth performance of S. platensis with the aim of replacing sodium bicarbonate (NaHCO3) and mineral nutrients in Kosaric medium (KM) with banana leaf ash extract. KM was used as control medium in treatment T1. In treatments T2 to T5, 50% of NaHCO3 in KM was replaced with banana leaf ash extract (BLAE). In addition, micronutrients in KM were reduced to 75%, 50% and 25% in treatments T3 to T5, respectively. The values of different physical and chemical parameters (temperature, pH, dissolved oxygen and light intensity) of the culture media were within the suitable range for S. platensis culture. Optical density in treatments T2 and T3 were similar to that was in treatment T1. Maximum cell dry weight (0.65 gL-1) was observed in treatment T1 and it was similar to the treatments T2 and T3 where 50% of NaHCO3 in KM was replaced with banana leaf ash extract with 100% and 75% micronutrients supply, respectively. The similar trend was found in chlorophyll a content. It was evident that 50% of NaHCO3 in KM can be replaced with BLAE for the culture of S. platensis, in addition micronutrients in KM can be reduced to 75%. Ann. Bangladesh Agric. (2019) 23(1) : 37-47

scholarly journals Distribution and isolation of microalgae for lipid production in selected freshwater reservoirs of northern Thailand

2018 ◽  
Vol 19 (1) ◽  
pp. 343-350
Author(s):  
TIPPAWAN PRASERTSIN ◽  
YUWADEE PEERAPORNPISAL
Keyword(s):  
Water Quality ◽  
Lipid Content ◽  
Trophic Status ◽  
Lipid Production ◽  
Dry Weight ◽  
Botryococcus Braunii ◽  
Chemical Parameters ◽  
Northern Thailand ◽  
Surrounding Areas ◽  
Physical And Chemical

Prasertsin T, Peerapornpisal Y. 2018. Distribution and isolation of microalgae for lipid production in selected freshwater reservoirs of northern Thailand. Biodiversitas 19: 343-350. Nong Bau Reservoir and Chiang Saen Lake are considered important freshwater reservoirs of Chiang Rai Province located in northern Thailand. The surrounding areas of these water bodies are host to a range of human activities that influence water quality. Moreover, to date, there are have not been any studies on the water quality and the distribution of microalgae in these places. The physical and chemical parameters of the water quality and microalgae were carried out in Nong Bua Reservoir and Chiang Saen Lake during the months of May, July, and October of 2015. Microalgae were isolated in order to investigate the lipid-producing abilities. Samples collected from Nong Bua Reservoir have revealed the presence of seven divisions, 90 species of algae. Six divisions, 55 species of algae were found in Chiang Saen Lake. The trophic status of the water was evaluated from the main parameters (AARL-PC Score), and it was determined that Nong Bua Reservoir was of meso-eutrophic status and Chiang Saen Lake was of mesotrophic status. Microalgae were isolated from Nong Bua Reservoir and Chiang Saen Lake for the purposes of studying lipid content; 25 and 6 isolations were identified, respectively. The lipid content was highest in Botryococcus braunii (39.25 ± 0.32% dry weight) followed by Ankistrodesmus sp. (26.80 ± 0.44% dry weight) and Coelastrum microsporum (24.95 ± 0.55% dry weight). The lowest lipid content was found in Planktolyngbya sp. (7.25 ± 0.43% dry weight). The indigenous strains of microalgae of Nong Bau Reservoir and Chiang Saen Lake’s can be considered quite promising as model strains in terms of the production of biofuel within the country.

scholarly journals Spirulina platensis Growth in Open Raceway Ponds Using Fresh Water Supplemented with Carbon, Nitrogen and Metal Ions

2003 ◽  
Vol 58 (1-2) ◽  
pp. 76-80 ◽  
Author(s):  
Jorge Alberto Vieira Costa ◽  
Luciane Maria Colla ◽  
Paulo Duarte Filho
Keyword(s):  
Sodium Bicarbonate ◽  
Metal Ions ◽  
Fresh Water ◽  
Spirulina Platensis ◽  
Ferric Iron ◽  
Dry Weight ◽  
Water Production ◽  
Rio Grande Do Sul ◽  
Urea Phosphate ◽  
Raceway Ponds

To investigate the feasibility of using fresh water from Mangueira Lagoon (Rio Grande do Sul, Brazil) for biomass production in open raceway ponds (0.7 m long, 0.18 m wide, 0.075 m deep) we studied the influence of nutrient addition (carbon as sodium bicarbonate, nitrogen as urea, phosphate, sulfate, ferric iron, magnesium and potassium) on the growth rate of the cyanobacteria Spirulina platensis using a 22 factorial design. In unsupplemented lagoon water production of S. platensis was 0.78 ± 0.01 g/l (dry weight basis) while the addition of 2.88 g/l of sodium bicarbonate (without added urea, phosphate, sulfate or metal ions) resulted in 0.82 ± 0.01 g/l after 400 hours of culture. The further addition of phosphate and metal ions resulted in growth for up to 750 h and a final S. platensis biomass of 1.23 ± 0.04 to 1.34 ± 0.03 g/l

scholarly journals Smart Production of Lipids as Bio-Fuel in Spirulina Platensis (=Arthrospira Fusiformis), and Bio-Oxygen and Bio-Electricity in Media Cultured in Supernatant of Digested Poultry Waste

2021 ◽  
Vol 04 (04) ◽  
Author(s):  
M.A. Satter ◽  
Keyword(s):  
Chlorophyll A ◽  
Spirulina Platensis ◽  
Culture Media ◽  
Total Biomass ◽  
Poultry Waste ◽  
Cell Weight ◽  
A Cell ◽  
The Media ◽  
Specific Growth Rates ◽  
Chlorophyll A Content

An experiment was conducted to evaluate growth performances and production of bio-fuel of Spirulina platensis (Gomont), and bio-oxygen and bio-electricity of culture media in supernatant of three different amount of digested poultry waste (DPW), and Kosaric medium (KM) as control. Three different amounts (concentrations) such as 2.0, 4.0 and 6.0 g/L poultry waste were allowed to digest under aeration. After 17 days, 700 ml grayish coloured supernatant was taken with addition of 9.0 g/L NaHCO3 and 0.50 ml/l micronutrient in 2.0 L conical flask with three replications and then autoclaved. Spirulina was inoculated to grow in these three treatments including KM (Control) after 72 hours of autoclave and then allowed to grow for a period of 14 days. This duration was estimated through repeated growth trials. The cell weight of spirulina was attained a maximum of 12.58 ± 1.25 mg/L (dry wt. basis) in KM followed by 11.46 ± 1.03, 9.16 ± 0.84 and 8.13 ± 0.73 mg/L in supernatant of 4.0, 2.0 and 6.0 g/L DPW, respectively on the 10th day of culture. Similar trend was also observed in the cases of optical density of the media contained spirulina, chlorophyll a content (mg/L), cell weight, total biomass (mg/l) and specific growth rates on the basis of cell weight and chlorophyll a. Cell weight of spirulina grown in these media had highly significant (P < 0.01) correlation with the chlorophyll a content (r = 0.993) of spirulina. Crude protein of spirulina grown in supernatant of DPW was lower than that of spirulina cultured in KM. Crude lipids as bio-fuel of spirulina cultured in supernatant of 4.0 g/L DPW was almost three times higher than that of spirulina grown in KM which may be due to high phospholipids bioaccumulation. Phosphate-P was decreased in media of DPW due to its use for high lipids biosynthesis as phospholipids. Bio-oxygen was produced higher ranged from 2.1 ± 11 mg/L on initial day to 10.20 ± 0.54 mg/L on 10th day grown in supernatant of 4.0 g/L DPW media. Bio-electricity as green electricity ranged from 135 ± 4 on initial day to 284 ± 7 mV on 10th day when spirulina cultured in supernatant of 4.0 g/L DPW which was higher than grown in other media. pH followed the similar trend like bio-oxygen and bio-electricity. It was found that the production of bio-oxygen, bio-fuel and bio-electricity had direct relation with pH in culture media of spirulina. Therefore, mass production of Spirulina platensis might be done in supernatant of 4.0 g/L digested poultry waste to get high total lipids as bio-fuel, bio-electricity and bio-oxygen.

scholarly journals Seasonal variation of biotic and abiotic parameters in parallel neotropical fishponds

2014 ◽  
Vol 74 (1) ◽  
pp. 166-174 ◽  
Author(s):  
LH Sipaúba-Tavares ◽  
LM Seto ◽  
RN Millan
Keyword(s):  
Water Quality ◽  
Phytoplankton Community ◽  
Similarity Analysis ◽  
Chemical Parameters ◽  
Abiotic Parameters ◽  
Physical And Chemical Parameters ◽  
One Year ◽  
Physical And Chemical ◽  
Chlorophyll A Content ◽  
Limnological Parameters

The present study was carried out to evaluate the phytoplankton community and water quality in four parallel fishponds (FP1 to FP4) during one year. Some abiotic parameters of water column and phytoplankton was sampled in four sites, one in each fishpond. Similarity analysis showed that FP2 fishpond was markedly different for the others ponds with regard to phytoplankton community. Conductivity, orthophosphate, temperature, TSS and hardness parameters were significantly different (p<0.05) between fishponds and all limnological parameters were significantly different (p<0.01) among period (months). Organic matter and phosphorus of the sediment were significantly different (p<0.01) only among fishponds. In current study Chlorophyceae and Bacillariophyceae occurred in most months. Cyanobacteria density in April and May was higher in all fishponds, at FP3 represented 71.4% in April and FP1 represented 78.7% in May of total reported, with chlorophyll-a content lower than 41 mg.L−1. The physical and chemical parameters of fishponds water constituted relations between the parameters and algae occurrence. The present study however, indicated that Cyanobacteria was more frequent in the fishponds after the fish grow-out period (April), and the water quality should be monitored more frequently in this period, due to the high total phosphorus, orthophosphate and chlorophyll-acontents.

Growth performance analysis of Spirulina platensis production by substituting K2SO4-K of kosaric medium with MOP-K

2019 ◽  
Vol 48 (3) ◽  
pp. 529-535
Author(s):  
Taslima Akter ◽  
Md Imdadul Haque ◽  
Mousumi Das ◽  
Md Amzad Hossain
Keyword(s):  
Chlorophyll A ◽  
Spirulina Platensis ◽  
Low Cost ◽  
Dry Weight ◽  
Control Treatment ◽  
Optical Cell ◽  
Cell Biomass ◽  
Significant Difference ◽  
Chlorophyll A Content ◽  
Muriate Of Potash

Five media were formulated with 0% (control, T1), 25% (T2), 50% (T3), 75% (T4) and 100% (T5) inclusion of low-cost muriate of potash (MOP)-potassium (K) replacing high-cost reagent K2SO4-K and Spirulina platensis was cultured for 18 days. Cell dry weight, optical cell density and chlorophyll-a content of S. platensis cultured in five treatments were registered at every three-day interval and economic performance was calculated to observe the effect of K2SO4-K replacement with MOP-K. The cell biomass production and chlorophyll-a content of S. platensis cultured in 25 and 50% use of MOP-K instead of K2SO4-K (T2 and T3) did not represent any significant difference with the control treatment of 100% K2SO4-K (T1). However, further addition of MOP-K in T4 and T5 significantly reduced the cell growth and pigment content of S. platensis. In addition, a significant reduction of production cost was calculated as more percentage of K2SO4- K was replaced with MOP-K.

The influence of gelatin and starter culture on the physical and chemical parameters of cheeses with different fat content

2018 ◽  
Vol 6 (10) ◽  
pp. 123-130
Author(s):  
Yaroslava Zhukova ◽  
◽  
Pylyp Petrov ◽  
Olena Boloba ◽  
Tetiana Ohrimenko ◽  
...  
Keyword(s):  
Starter Culture ◽  
Fat Content ◽  
Chemical Parameters ◽  
Physical And Chemical Parameters ◽  
Physical And Chemical

Eliminating Thrips in Microshoot Cultures

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506d-506
Author(s):  
Robert R. Tripepi ◽  
Holly J. Schwager ◽  
Mary W. George ◽  
Joseph P. McCaffrey
Keyword(s):  
Betula Pendula ◽  
Culture Media ◽  
Dry Weight ◽  
Thrips Tabaci ◽  
Shoot Cultures ◽  
Onion Thrips ◽  
Ulmus Americana ◽  
Shoot Height ◽  
Tissue Culture Media ◽  
Plant Tissue Cultures

Two insecticides, acephate or azadirachtin, were added to tissue culture media to determine their effectiveness in controlling onion thrips (Thrips tabaci Lindeman.) and to determine if these insecticides could damage the plant shoot cultures. To test for insecticide phytotoxicity, microshoots from European birch (Betula pendula), American elm (Ulmus americana), `Pink Arola' chrysanthemum (Dendranthema grandiflora), `America' rhododendron (Rhododendron catawbiense), `Golden Emblem' rose (Rosa hybrida), and `Gala' apple (Malus domestica) were placed in 130-ml baby food jars containing 25 ml of medium supplemented with 6.5, 13, or 26 mg/l Orthene® (contained acephate) or 0.55, 1.1, or 2.2 ml/l Azatin® (contained azadirachtin). Control jars lacked insecticide. To test for thrips control, 13 mg/l Orthene® or 0.55 ml/l Azatin® was added to Murashige and Skoog medium, and 10 thrips were placed on `Gala' apple microshoots in each jar. Jars were sealed with plastic wrap. In both studies, microshoot dry weight and heights were determined. In the second study, the total number of thrips per jar was also determined 3 weeks after inoculation. Microshoots on Orthene®-treated media lacked phytotoxicity symptoms, regardless of the concentration used. In contrast, Azatin® hindered plant growth, decreasing shoot height or dry weight by up to 85% depending on the species. Both insecticides prevented thrips populations from increasing, since less than 10 thrips were found in jars with insecticide-treated medium. Control jars, however, contained an average of almost 70 thrips per jar. This study demonstrated that both Orthene® and Azatin® were effective for eradicating thrips from plant tissue cultures, but Orthene® should probably be used because Azatin® was phytotoxic to all species tested.

Life in Glacial and Alpine Rivers in Central Iceland in Relation to Physical and Chemical Parameters

2000 ◽  
Vol 31 (4-5) ◽  
pp. 411-422 ◽  
Author(s):  
Gísli Már Gíslason ◽  
Jón S. Ólafsson ◽  
Hákon Adalsteinsson
Keyword(s):  
Chemical Properties ◽  
Chemical Parameters ◽  
Invertebrate Fauna ◽  
Glacial Meltwater ◽  
Biological Communities ◽  
Relative Contribution ◽  
Glacial River ◽  
Alpine Rivers ◽  
Physical And Chemical ◽  
And Chemical Properties

The characteristics of stream and river ecosystems in arctic and alpine areas are determined mainly by the relative contribution of glacial meltwater, snowmelt, rainfall and groundwater. Each source generates a particular seasonal hydrological signature, affecting physical and chemical properties, and hence biological communities. The relative contribution of each source is sensitive to climate change. The study was concentrated on the glacial River W-Jökulsá and some non-glacial rivers in the central highlands of Iceland. The water in the glacial river was entirely glacial meltwater at the glacier margin, but the glacial contribution was about 20% 40 km downstream. However, its tributaries and non-glacial reference rivers were mainly springfed. The invertebrate fauna was confined to Chironomidae of the genus Diamesa close to the glacier, but other taxa (species and groups of species) occupied the river further downstream, where their diversity was close to that found in the reference rivers.

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