scholarly journals Collection, preservation and forwarding of biological samples for toxicological analysis in medico legal autopsy cases

2019 ◽  
Vol 1 (Number 2) ◽  
pp. 25-28
Author(s):  
Utpal Kumar Biswas ◽  
Nashid Tabassum Khan ◽  
Mohammad Ahad Hossain ◽  
Abdul Kader
Keyword(s):  
Biological Samples ◽  
Forensic Pathologist ◽  
Biological Specimen ◽  
Standard Methods ◽  
Toxicological Analysis ◽  
Essential Step ◽  
Specimen Collection ◽  
Autopsy Specimen ◽  
Case Work

Collection of proper autopsy specimen is an essential step in the process of toxicology case work. Improper collection of these specimens can greatly alter or negate chemical and toxicological analysis. This article is an update about the standard methods of biological specimen collection procedures for toxicological analysis which will be helpful for the forensic pathologist and forensicscientists.

scholarly journals Forensic toxicological analysis of chemical warfare agents and their metabolites in biological samples

2014 ◽  
Vol 28 (2) ◽  
pp. 154-163
Author(s):  
Ismail Ethem Goren ◽  
Nebile Daglioglu ◽  
Pinar Efeoglu ◽  
Mete Korkut Gulmen
Keyword(s):  
Biological Samples ◽  
Chemical Warfare Agents ◽  
Chemical Warfare ◽  
Toxicological Analysis ◽  
Warfare Agents

Development of a novel method for the bioanalysis of benfotiamine and sulbutiamine in cancer cells

2016 ◽  
Vol 8 (28) ◽  
pp. 5596-5603 ◽  
Author(s):  
Jaeah Kim ◽  
Christopher P. Hopper ◽  
Kelsey H. Connell ◽  
Parisa Darkhal ◽  
Jason A. Zastre ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Biological Samples ◽  
Cancer Cell Proliferation ◽  
Essential Step ◽  
Novel Method

Quantification of benfotiamine and sulbutiamine, synthetic thiamine analogs, in biological samples is an essential step toward understanding the role of these thiamine analogs on cancer cell proliferation.

Cryofixation of various biological samples by high pressure freezing

1992 ◽  
Vol 50 (1) ◽  
pp. 752-753
Author(s):  
LUCY RU-SIU YIN
Keyword(s):  
High Pressure ◽  
Biological Samples ◽  
High Rate ◽  
Crystal Formation ◽  
Chemical Fixation ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Native State ◽  
Biological Specimen ◽  
Ice Crystal Formation

The ultimate aim of ultrastructural fixation of biological specimen is to preserve all the compartments in their native state. Cryofixation is a superior method than conventional chemical fixation in reaching this goal. However, ice crystal formation during cryofixation often damages the structures. High pressure (2100 bar) freezing provides a way to alter freezing properties while cool down the specimen at a relatively high rate, minimizing the ice crystal formation. Nearly vitrified samples(up to 500 um) have been obtained with this method. Samples in suspension tend to get lost during high pressure freezing. The low percentage (∼30%) of successfully cryofixed specimens can be improved if the sample completely fills the cavity of the metal specimen carriers in which the specimen is frozen. Various methods to overcome sample loss are reported in this study.

Biological specimen preparation for SEM by a method other than critical point drying

1987 ◽  
Vol 45 ◽  
pp. 956-957 ◽  
Author(s):  
J. L. Adams ◽  
C. J. Battjes ◽  
D. A. Buthala
Keyword(s):  
Sample Preparation ◽  
Critical Point ◽  
Biological Samples ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Specimen Preparation ◽  
Biological Specimen ◽  
Air Drying ◽  
Critical Point Drying ◽  
Good Preparation

Quality sample preparation for SEM is important to observe fine details without artifacts, and good preparation requires proper fixation, dehydration, drying and coating, An alternative 5 min passage in hexamethyldisilazane (HMDS) can replace critical point crying (CPD) and gives satisfactory results on many biological samples. CPD procedure may take up to 1 h per sample to ensure adequate drying, therefore a brief rinse in HMDS followed by air drying requires less time and equipment yet provides excellent results.Various biological samples were fixed in 3% glutaraldehyde; rinsed 3 times in Millonig's phosphate buffer for 10 min each; post-fixed in 1% osmium tetroxide for 1 h; rinsed as before; fixed again in 1% tannic acid (TA) for 30 min-1 h; rinsed well and partially dehydrated to 70% ethanol; placed in 1% uranyl-acetate (UA) in the dark, overnight: rinsed with 70% ethanol until UA cleared and then dehydrated through 100% ethanol.

Chromium Manganese and Zinc Levels in the Biological Samples of Type 1 Diabetic Mellitus Children Reside in Different Areas of Sindh Pakistan

2021 ◽  
Vol 43 (3) ◽  
pp. 260-260
Author(s):  
Anjum Lashari Anjum Lashari ◽  
Hasan Imran Afridi Hasan Imran Afridi ◽  
Tasneem Gul Kazi Tasneem Gul Kazi ◽  
Farah Naz Talpur and Jameel Ahmed Baig Farah Naz Talpur and Jameel Ahmed Baig
Keyword(s):  
Biological Samples ◽  
Scalp Hair ◽  
Sample Pretreatment ◽  
Age Group ◽  
Diabetic Mellitus ◽  
Serum Samples ◽  
Biological Specimen ◽  
Diabetic Population ◽  
Zinc Levels

T1DM is the most acquainted childhood immune-caused onset and may lead to early mortalities and morbidities. It can emanate in any age group but is highest reported in lesser than 18 years of age. In Pakistan only, T1DM institutes ~ 2% of the all diabetic population. This work was formulated to assess the concentration of manganese (Mn), zinc (Zn) and chromium (Cr) in blood, scalp hair and serum samples of diabetic mellitus type 1 children age ranged (1–5), (6-10) and (11-14) years of both genders (male and female), compared with similar sex and age referent subjects. For sample pretreatment, microwave-aided acid digestion procedure (MAD) was employed for elemental analysis in the biological samples of T1DM children. The employed method was validated by using certified reference samples of blood, scalp hair, and serum. Digests biological specimen were analyzed by AAS. The Zn was analyzed by FAAS, while the assessment of Mn and Cr were measured by ETAAS. The Mn, Zn and Cr, presents significantly low levels in the samples of scalp hair, blood and serum of female and male of age group of (1-5) (6-10) and (11-14) of diabetic mellitus type 1 children, compared with referent subjects. These data present an important hypothesis to doctors and other clinical experts to investigate insufficiency of these essential micronutrients (Cr, Mn, andamp; Zn) in biological specimen of blood, scalp hairs and serum of T1DM children.

Feasibility of Biological Specimen Collection for the Canadian Longitudinal Study on Aging (CLSA) Biorepository

2009 ◽  
Vol 28 (3) ◽  
pp. 261-274 ◽  
Author(s):  
Cynthia M. Balion ◽  
Parminder S. Raina ◽  
Christina Wolfson ◽  
Susan A. Kirkland ◽  
Judy L. Keys ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Nous Avons ◽  
Biological Specimen ◽  
Specimen Collection

RÉSUMÉLa collecte de spécimens biologiques est une partie intégrale de beaucoup d’études épidémiologiques longitudinales. Il est important d’obtenir un haut taux de satisfaction de la part des participants pour que leur participation soit continue et pour assurer une qualité élevée des échantillons pour avoir des mesures précises pour les biomarqueurs. Nous avons réalisé une étude pour évaluer ces questions sur la collecte d’échantillons proposée pour l’Étude longitudinale canadienne sur le vieillissement (ÉLCV). Parmi les 85 participants recrutés, 65 ont été dirigés vers un laboratoire d’hôpital ou un laboratoire privé. Environ 100 mL de sang et un prélèvement aléatoire d’urine ont été collectés pour chaque participant, pour un total de 2 108 aliquots d’échantillon. Les niveaux de qualité ont été atteints pour plus de 90 % des échantillons et étaient semblables pour les échantillons collectés dans les deux laboratoires. Plus de 90 % des participants ont exprimé que leur satisfaction par rapport à la collecte était bonne ou excellente, et 84 % serait prêts à répéter la collecte dans un à trois ans.

scholarly journals Review on Collection , Preservation and Fowarding of Biological Samples for Toxicological Analysis

2018 ◽  
Vol 22 (1) ◽  
pp. 431-438
Author(s):  
Maha Hilal ◽  
Essam Abdullah ◽  
Reda El Sayed ◽  
Hend Salman
Keyword(s):  
Biological Samples ◽  
Toxicological Analysis
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