First molecular detection of Tritrichomonas foetus in domestic cats in Klang Valley, Malaysia

ABSTRACT: Felid alphaherpesvirus 1 (FeHV-1) and feline calicivirus (FCV) affect cats worldwide. The aim of this study was to evaluate the frequency of occurrence of FeHV-1 and FCV in cats with clinical signs of respiratory, oral and/or ocular disease. Samples were collected from cats cared for in veterinary ambulatory and clinics and submitted to molecular detection and viral isolation. Of the 49 cats evaluated, 45 (92%) were positive for at least one of the viruses; 82% (40/49) were positive for FeHV-1 and 41% (20/49) for FCV. Of these, 31% (15/49) were coinfection cases. For FeHV-1, 45% (18/40) of the cats tested were positive from the collection of eye swab, and the same percentage (9/20) was obtained for the FCV by the oral swab. FeHV-1 and/or FCV were isolated in 35% (17/49) of the samples. The main clinical sign observed was ocular secretion in 71% (35/49) of cats, characterized as mild serous, purulent or serosanguineous, and in some cases associated with ocular injury and marked chemosis. Our findings demonstrate the high occurrence of FeHV-1 and FCV in domestic cats in southern Brazil and indicate that measures should be implemented to improve the diagnostic, prevention and management against of these important diseases.

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Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638717720245 ◽

2017 ◽

Vol 29 (5) ◽

pp. 660-668 ◽

Cited By ~ 1

Author(s):

Mariana M. Furtado ◽

Sueli A. Taniwaki ◽

Iracema N. de Barros ◽

Paulo E. Brandão ◽

José L. Catão-Dias ◽

...

Keyword(s):

Feline Immunodeficiency Virus ◽

Molecular Detection ◽

Puma Concolor ◽

Leukemia Virus ◽

Feline Leukemia Virus ◽

Molecular Testing ◽

Domestic Cats ◽

Immunodeficiency Virus ◽

Free Ranging ◽

Feline Coronavirus

We describe molecular testing for felid alphaherpesvirus 1 (FHV-1), carnivore protoparvovirus 1 (CPPV-1), feline calicivirus (FCV), alphacoronavirus 1 (feline coronavirus [FCoV]), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and canine distemper virus (CDV) in whole blood samples of 109 free-ranging and 68 captive neotropical felids from Brazil. Samples from 2 jaguars ( Panthera onca) and 1 oncilla ( Leopardus tigrinus) were positive for FHV-1; 2 jaguars, 1 puma ( Puma concolor), and 1 jaguarundi ( Herpairulus yagouaroundi) tested positive for CPPV-1; and 1 puma was positive for FIV. Based on comparison of 103 nucleotides of the UL24-UL25 gene, the FHV-1 sequences were 99–100% similar to the FHV-1 strain of domestic cats. Nucleotide sequences of CPPV-1 were closely related to sequences detected in other wild carnivores, comparing 294 nucleotides of the VP1 gene. The FIV nucleotide sequence detected in the free-ranging puma, based on comparison of 444 nucleotides of the pol gene, grouped with other lentiviruses described in pumas, and had 82.4% identity with a free-ranging puma from Yellowstone Park and 79.5% with a captive puma from Brazil. Our data document the circulation of FHV-1, CPPV-1, and FIV in neotropical felids in Brazil.

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The molecular detection of relaxin and its receptor RXFP1 in reproductive tissue of Felis catus and Lynx pardinus during pregnancy

Reproduction ◽

10.1530/rep-11-0316 ◽

2012 ◽

Vol 143 (3) ◽

pp. 399-410 ◽

Cited By ~ 18

Author(s):

Beate C Braun ◽

Astrid Vargas ◽

Katarina Jewgenow

Keyword(s):

Mrna Expression ◽

Molecular Detection ◽

Felis Catus ◽

Specific Information ◽

Reproductive Tissue ◽

Domestic Cats ◽

Uterine Tissue ◽

Iberian Lynx ◽

Lynx Pardinus ◽

Relaxin Receptor

Relaxin acts as a pregnancy-specific signal in feline species, but specific information about protein structure and binding is essential for the improvement of pregnancy diagnosis in endangered feline species, like the Iberian lynx. To generate a felid-specific relaxin antibody, the DNA and protein sequences of lynx and cat were determined and peptides were chosen for antibody generation. In addition, relaxin and relaxin receptor (RXFP1) mRNA expressions were measured in uteri and ovaries of pregnant domestic cats and lynx placentae. Using real-time PCR and immunohistochemistry, it was established that feline placenta is the main source of relaxin during pregnancy. In other tested tissues, relaxin mRNA expression was weak. TheRXFP1mRNA expression was found mainly in cat uterine tissue and feline placentae. It was assumed that these tissues were main targets for relaxin. In the ovary, relaxin immunostaining was associated with blood vessels, signifying its role in vascularization.

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Draft Genome Sequence of Tritrichomonas foetus Strain K

Genome Announcements ◽

10.1128/genomea.00195-17 ◽

2017 ◽

Vol 5 (16) ◽

Cited By ~ 5

Author(s):

Marlene Benchimol ◽

Luiz G. P. de Almeida ◽

Ana Tereza Vasconcelos ◽

Ivone de Andrade Rosa ◽

Maurício Reis Bogo ◽

...

Keyword(s):

Genome Sequence ◽

Venereal Disease ◽

Draft Genome ◽

Draft Genome Sequence ◽

Tritrichomonas Foetus ◽

Domestic Cats

ABSTRACT The protist Tritrichomonas foetus (Excavata, Parabasalia) is a parasite that causes bovine and feline trichomonosis. Bovine trichomonosis is a venereal disease that leads to abortion and reproductive problems in herds. Feline trichomonosis affects domestic cats. Here, we report the genome sequence of the T. foetus K strain, isolated in Brazil.

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Molecular detection, characterization of Anaplasma spp. in domestic cats from Rio de Janeiro state

Acta Tropica ◽

10.1016/j.actatropica.2019.01.003 ◽

2019 ◽

Vol 191 ◽

pp. 239-242 ◽

Cited By ~ 2

Author(s):

Andresa Guimarães ◽

Juliana Macedo Raimundo ◽

Maristela Peckle Peixoto ◽

Claudia Bezerra da Silva ◽

Marcus Sandes Pires ◽

...

Keyword(s):

Rio De Janeiro ◽

Molecular Detection ◽

Domestic Cats ◽

Janeiro State

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Molecular detection of Mycoplasma haemofelis in domestic cats from municipality of mineiros, Goiás

Brazilian Journal of Development ◽

10.34117/bjdv6n5-021 ◽

2020 ◽

Vol 6 (5) ◽

pp. 23084-23093

Author(s):

Francielma Rezende Silva ◽

Karla Irigaray Nogueira Borges ◽

Stéfhano Luis Candido ◽

Valéria Dutra ◽

Raphaella Barbosa Meirelles Bartoli ◽

...

Keyword(s):

Molecular Detection ◽

Domestic Cats

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Detection of enteric agents into a cats’ shelter with cases of chronic diarrhea in Southern Brazil

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5987 ◽

2019 ◽

Vol 39 (8) ◽

pp. 630-634

Author(s):

Ana Cristina S. Mósena ◽

Dafne L. Cruz ◽

Cláudio W. Canal ◽

Sandra M.T. Marques ◽

Stella F. Valle ◽

...

Keyword(s):

Viral Infections ◽

Chronic Diarrhea ◽

Free Access ◽

Tritrichomonas Foetus ◽

Southern Brazil ◽

Domestic Cats ◽

Factors Associated ◽

Cryptosporidium Spp ◽

Helminth Eggs ◽

First Finding

ABSTRACT: This study carried out a survey about enteropathogenic agents in domestic cats’ shelter as a stage of investigation for the intermittent chronic diarrhea. Individual fecal samples from 39 cats with free access to the external environment were submitted to parasitological examination, parvovirus, and coronavirus by PCR, and Cryptosporidium spp., Giardia spp. and Tritrichomonas foetus by real-time PCR. From the cats evaluated, 30 (76.9%) were positive for one or more enteric agents, and coinfections were observed in 11 cats samples (28.2%). Helminth eggs were observed in 48.7% of cats (19/30), 16 (41%) were positive for parvovirus or coronavirus and 25.6% (10/30) were infected by protozoa. From the positives for protozoa, five cats were positive to T. foetus (12.82%). The first finding of this protozoan through PCR was in the southern Brazil, and the second was in the whole country. Chronic diarrhea in cats may be multifactorial in shelter animals where the population density is high and the control of parasitic, and viral infections are deficient. Moreover, it is due to poor hygiene conditions in these shelters. The factors associated with the proliferation of infectious diseases in shelters are correlated with new pathogens infections such as T. foetus.

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Molecular Detection and Genetic Diversity of Toxoplasma gondii Oocysts in Cat Faeces from Klang Valley, Malaysia, Using B1 and REP Genes in 2018

Pathogens ◽

10.3390/pathogens9070576 ◽

2020 ◽

Vol 9 (7) ◽

pp. 576 ◽

Cited By ~ 2

Author(s):

Mohammed Nasiru Wana ◽

Mohamad Aris Mohd Moklas ◽

Malaika Watanabe ◽

Ngah Zasmy Unyah ◽

Sharif Alhassan Abdullahi ◽

...

Keyword(s):

Genetic Diversity ◽

Toxoplasma Gondii ◽

Molecular Detection ◽

Positive Sample ◽

Perfect Agreement ◽

B1 Gene ◽

Coccidian Parasites ◽

Major Route ◽

Klang Valley ◽

Pcr Assays

The major route for Toxoplasma gondii (T. gondii) infection is through the ingestion of foods contaminated with oocyst from cat faeces. The microscopic detection of T. gondii oocysts in cat faeces is challenging, which contributes to the failure of detecting or differentiating it from other related coccidian parasites. This study aims to detect T. gondii oocysts in cat faeces using two multicopy-target PCR assays and to evaluate their genetic diversity. Cat faecal (200) samples were collected from pet cats (PCs; 100) and free-roaming cats (FRCs; 100) within Klang Valley, Malaysia, and screened for coccidian oocysts by microscopy using Sheather’s sucrose floatation. PCR assays were performed on each faecal sample, targeting a B1 gene and a repetitive element (REP) gene to confirm T. gondii oocysts. Additionally, the PCR amplicons from the REP gene were sequenced to further confirm T. gondii-positive samples for phylogenetic analysis. Microscopy detected 7/200 (3.5%) T. gondii-like oocysts, while both the B1 gene and the REP gene detected 17/200 (8.5%) samples positive for T. gondii. All samples that were microscopically positive for T. gondii-like oocysts were also shown to be positive by both B1 and REP genes. The BLAST results sequenced for 16/200 (8.0%) PCR-positive T. gondii samples revealed homology and genetic heterogeneity with T. gondii strains in the GenBank, except for only one positive sample that did not show a result. There was almost perfect agreement (k = 0.145) between the two PCR assays targeting the B1 gene and the REP gene. This is the first report on microscopic, molecular detection and genetic diversity of T. gondii from cat faecal samples in Malaysia. In addition, the sensitivities of either the B1 gene or REP gene multicopy-target PCR assays are suitable for the accurate detection of T. gondii from cat faeces.

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Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612014039 ◽

2014 ◽

Vol 23 (2) ◽

pp. 231-236 ◽

Cited By ~ 11

Author(s):

Ana Cláudia Gabriela Alexandre de Santis ◽

Heitor Miraglia Herrera ◽

Keyla Cartens Marques de Sousa ◽

Luiz Ricardo Gonçalves ◽

Nathani Cristina Baccarim Denardi ◽

...

Keyword(s):

Blood Cells ◽

Molecular Detection ◽

Clinical Signs ◽

Molecular Techniques ◽

Domestic Cats ◽

Mato Grosso ◽

Blast Analysis ◽

Significant Difference ◽

Pcr Assays ◽

Mato Grosso Do Sul

Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for ‘Candidatus Mycoplasma haemominutum’, 17 (11.2%) for M. haemofelis and 15 (9.9%) for ‘Candidatus M. turicensis’, from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.

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