scholarly journals Molecular detection of Mycoplasma haemofelis in domestic cats from municipality of mineiros, Goiás

2020 ◽  
Vol 6 (5) ◽  
pp. 23084-23093
Author(s):  
Francielma Rezende Silva ◽  
Karla Irigaray Nogueira Borges ◽  
Stéfhano Luis Candido ◽  
Valéria Dutra ◽  
Raphaella Barbosa Meirelles Bartoli ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Domestic Cats

scholarly journals High occurrence of felid alphaherpesvirus 1 and feline calicivirus in domestic cats from southern Brazil

2020 ◽  
Vol 40 (9) ◽  
pp. 685-689
Author(s):  
Alice S. Becker ◽  
Francielle Liz Monteiro ◽  
Ana Carolina A. Scariot ◽  
Domitila B. Chagas ◽  
Geferson Fischer ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Clinical Signs ◽  
Feline Calicivirus ◽  
Ocular Injury ◽  
Frequency Of Occurrence ◽  
Southern Brazil ◽  
Domestic Cats ◽  
Oral Swab ◽  
Viral Isolation ◽  
High Occurrence

ABSTRACT: Felid alphaherpesvirus 1 (FeHV-1) and feline calicivirus (FCV) affect cats worldwide. The aim of this study was to evaluate the frequency of occurrence of FeHV-1 and FCV in cats with clinical signs of respiratory, oral and/or ocular disease. Samples were collected from cats cared for in veterinary ambulatory and clinics and submitted to molecular detection and viral isolation. Of the 49 cats evaluated, 45 (92%) were positive for at least one of the viruses; 82% (40/49) were positive for FeHV-1 and 41% (20/49) for FCV. Of these, 31% (15/49) were coinfection cases. For FeHV-1, 45% (18/40) of the cats tested were positive from the collection of eye swab, and the same percentage (9/20) was obtained for the FCV by the oral swab. FeHV-1 and/or FCV were isolated in 35% (17/49) of the samples. The main clinical sign observed was ocular secretion in 71% (35/49) of cats, characterized as mild serous, purulent or serosanguineous, and in some cases associated with ocular injury and marked chemosis. Our findings demonstrate the high occurrence of FeHV-1 and FCV in domestic cats in southern Brazil and indicate that measures should be implemented to improve the diagnostic, prevention and management against of these important diseases.

scholarly journals Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil

2017 ◽  
Vol 29 (5) ◽  
pp. 660-668 ◽  
Author(s):  
Mariana M. Furtado ◽  
Sueli A. Taniwaki ◽  
Iracema N. de Barros ◽  
Paulo E. Brandão ◽  
José L. Catão-Dias ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Molecular Detection ◽  
Puma Concolor ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Molecular Testing ◽  
Domestic Cats ◽  
Immunodeficiency Virus ◽  
Free Ranging ◽  
Feline Coronavirus

We describe molecular testing for felid alphaherpesvirus 1 (FHV-1), carnivore protoparvovirus 1 (CPPV-1), feline calicivirus (FCV), alphacoronavirus 1 (feline coronavirus [FCoV]), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and canine distemper virus (CDV) in whole blood samples of 109 free-ranging and 68 captive neotropical felids from Brazil. Samples from 2 jaguars ( Panthera onca) and 1 oncilla ( Leopardus tigrinus) were positive for FHV-1; 2 jaguars, 1 puma ( Puma concolor), and 1 jaguarundi ( Herpairulus yagouaroundi) tested positive for CPPV-1; and 1 puma was positive for FIV. Based on comparison of 103 nucleotides of the UL24-UL25 gene, the FHV-1 sequences were 99–100% similar to the FHV-1 strain of domestic cats. Nucleotide sequences of CPPV-1 were closely related to sequences detected in other wild carnivores, comparing 294 nucleotides of the VP1 gene. The FIV nucleotide sequence detected in the free-ranging puma, based on comparison of 444 nucleotides of the pol gene, grouped with other lentiviruses described in pumas, and had 82.4% identity with a free-ranging puma from Yellowstone Park and 79.5% with a captive puma from Brazil. Our data document the circulation of FHV-1, CPPV-1, and FIV in neotropical felids in Brazil.

scholarly journals The molecular detection of relaxin and its receptor RXFP1 in reproductive tissue of Felis catus and Lynx pardinus during pregnancy

Reproduction ◽  
2012 ◽  
Vol 143 (3) ◽  
pp. 399-410 ◽  
Author(s):  
Beate C Braun ◽  
Astrid Vargas ◽  
Katarina Jewgenow
Keyword(s):  
Mrna Expression ◽  
Molecular Detection ◽  
Felis Catus ◽  
Specific Information ◽  
Reproductive Tissue ◽  
Domestic Cats ◽  
Uterine Tissue ◽  
Iberian Lynx ◽  
Lynx Pardinus ◽  
Relaxin Receptor

Relaxin acts as a pregnancy-specific signal in feline species, but specific information about protein structure and binding is essential for the improvement of pregnancy diagnosis in endangered feline species, like the Iberian lynx. To generate a felid-specific relaxin antibody, the DNA and protein sequences of lynx and cat were determined and peptides were chosen for antibody generation. In addition, relaxin and relaxin receptor (RXFP1) mRNA expressions were measured in uteri and ovaries of pregnant domestic cats and lynx placentae. Using real-time PCR and immunohistochemistry, it was established that feline placenta is the main source of relaxin during pregnancy. In other tested tissues, relaxin mRNA expression was weak. TheRXFP1mRNA expression was found mainly in cat uterine tissue and feline placentae. It was assumed that these tissues were main targets for relaxin. In the ovary, relaxin immunostaining was associated with blood vessels, signifying its role in vascularization.

Molecular detection, characterization of Anaplasma spp. in domestic cats from Rio de Janeiro state

Acta Tropica ◽  
2019 ◽  
Vol 191 ◽  
pp. 239-242 ◽  
Author(s):  
Andresa Guimarães ◽  
Juliana Macedo Raimundo ◽  
Maristela Peckle Peixoto ◽  
Claudia Bezerra da Silva ◽  
Marcus Sandes Pires ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Molecular Detection ◽  
Domestic Cats ◽  
Janeiro State

scholarly journals Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Ana Cláudia Gabriela Alexandre de Santis ◽  
Heitor Miraglia Herrera ◽  
Keyla Cartens Marques de Sousa ◽  
Luiz Ricardo Gonçalves ◽  
Nathani Cristina Baccarim Denardi ◽  
...  
Keyword(s):  
Blood Cells ◽  
Molecular Detection ◽  
Clinical Signs ◽  
Molecular Techniques ◽  
Domestic Cats ◽  
Mato Grosso ◽  
Blast Analysis ◽  
Significant Difference ◽  
Pcr Assays ◽  
Mato Grosso Do Sul

Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for ‘Candidatus Mycoplasma haemominutum’, 17 (11.2%) for M. haemofelis and 15 (9.9%) for ‘Candidatus M. turicensis’, from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.

Molecular detection of tick-borne protozoan parasites in a population of domestic cats in midwestern Brazil

2016 ◽  
Vol 7 (5) ◽  
pp. 1004-1009 ◽  
Author(s):  
Ísis Assis Braga ◽  
Dirceu Guilherme de Souza Ramos ◽  
Arlei Marcili ◽  
Andréia Lima Tomé Melo ◽  
Isis Indaiara Gonçalves Granjeiro Taques ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Domestic Cats ◽  
Protozoan Parasites

scholarly journals Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

2017 ◽  
Vol 26 (4) ◽  
pp. 505-510 ◽  
Author(s):  
Anna Claudia Baumel Mongruel ◽  
Jyan Lucas Benevenute ◽  
Priscila Ikeda ◽  
Marcos Rogério André ◽  
Rosangela Zacarias Machado ◽  
...  
Keyword(s):  
Wild Bird ◽  
Molecular Detection ◽  
Bird Species ◽  
Rrna Gene ◽  
Wild Animals ◽  
Domestic Cats ◽  
Free Living ◽  
Likelihood Analysis ◽  
Pcr Targeting ◽  
The 16S Rrna Gene

Abstract Wild animals play an important role in carrying vectors that may potentially transmit pathogens. Several reports highlighted the participation of wild animals on the Anaplasma phagocytophilum cycle, including as hosts of the agent. The aim of this study was to report the molecular detection of an agent phylogenetically related to A. phagocytophilum isolated from a wild bird in the Midwest of the state of Paraná, Brazil. Fifteen blood samples were collected from eleven different bird species in the Guarapuava region. One sample collected from a Penelope obscura bird was positive in nested PCR targeting the 16S rRNA gene of Anaplasma spp. The phylogenetic tree based on the Maximum Likelihood analysis showed that the sequence obtained was placed in the same clade with A. phagocytophilum isolated from domestic cats in Brazil. The present study reports the first molecular detection of a phylogenetically related A. phagocytophilum bacterium in a bird from Paraná State.

scholarly journals Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil

2019 ◽  
Vol 28 (1) ◽  
pp. 180-185 ◽  
Author(s):  
Andresa Guimarães ◽  
Juliana Macedo Raimundo ◽  
Raisa Braul Rodrigues ◽  
Maristela Peckle Peixoto ◽  
Huarrisson Azevedo Santos ◽  
...  
Keyword(s):  
16S Rrna ◽  
Rio De Janeiro ◽  
Molecular Detection ◽  
Rrna Gene ◽  
Igg Antibodies ◽  
Domestic Cats ◽  
Rickettsial Infection ◽  
Hematological Changes ◽  
Rrna Sequences ◽  
Janeiro State

Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.

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