CLINICAL AND PATHOGENESIS OVERVIEW OF MYROIDES SPP. INFECTION

The genus Myroides classified as Flavobacterium odoratum comprises non- motile ,aerobic, yellow –pigmented .They are non hemolytic on blood agar while it show a good growth on MacConkey and nutrient agar with salt tolerant up to 5% of NaCl also non fermented gram-negative rods .Members of the genus are cause many infectious disease in severely immunocompromised patient includes endocarditics urinary tract infection ,cuteneous infection ,and ventriculitis that behave as low grade opportunistic pathogens and widely distributed in the environment specially in water. Myroides spp. should be attribute among pathogens in hospitalized ,catheterized patients with wide resistant to antibiotic specially Minocycline is useful treating UTI caused by the genus . 16S ribosomal RNA(rRNA) sequencing can identified accurately the species level .In this reviews which description of dangerous infection duo to by Myroides in an immunocompromise host ,moreover reviewed the antibiotic resistance and pathogenesis. Many species of the genus Myroides were primarily taking from intestine of human. The genus Myroides includes five species : M. odoratus. , M. pelagicus, M. profundi , M. odoratimimus and M. marinus , by susceptibility to desferrioxamine . M. profundi has produced a protease Myroilysin ,with characterized as playing a role in hydrolysis of collagen through collagenase production and elastinolytic activity displaying by metalloprotease and playing important role with collagenase for hydrolysis of collagen that proven virulence of genus.

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Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections

Antibiotics ◽

10.3390/antibiotics10060721 ◽

2021 ◽

Vol 10 (6) ◽

pp. 721

Author(s):

John E. Romanowski ◽

Shannon V. Nayyar ◽

Eric G. Romanowski ◽

Vishal Jhanji ◽

Robert M. Q. Shanks ◽

...

Keyword(s):

Empiric Therapy ◽

Species Level ◽

Opportunistic Pathogens ◽

Coagulase Negative Staphylococci ◽

Biochemical System ◽

Ocular Infections ◽

Antibiotic Susceptibilities ◽

System 2 ◽

Intravitreal Treatment ◽

Susceptibility Profiles

Coagulase-negative staphylococci (CoNS) are frequently occurring ocular opportunistic pathogens that are not easily identifiable to the species level. The goal of this study was to speciate CoNS and document antibiotic susceptibilities from cases of endophthalmitis (n = 50), keratitis (n = 50), and conjunctivitis/blepharitis (n = 50) for empiric therapy. All 150 isolates of CoNS were speciated using (1) API Staph (biochemical system), (2) Biolog GEN III Microplates (phenotypic substrate system), and (3) DNA sequencing of the sodA gene. Disk diffusion antibiotic susceptibilities for topical and intravitreal treatment were determined based on serum standards. CoNS identification to the species level by all three methods indicated that S. epidermidis was the predominant species of CoNS isolated from cases of endophthalmitis (84–90%), keratitis (80–86%), and conjunctivitis/blepharitis (62–68%). Identifications indicated different distributions of CoNS species among endophthalmitis (6), keratitis (10), and conjunctivitis/blepharitis (13). Antibiotic susceptibility profiles support empiric treatment of endophthalmitis with vancomycin, and keratitis treatment with cefazolin or vancomycin. There was no clear antibiotic choice for conjunctivitis/blepharitis. S. epidermidis was the most frequently found CoNS ocular pathogen, and infection by other CoNS appears to be less specific and random. Antibiotic resistance does not appear to be a serious problem associated with CoNS.

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Hydrolysis of TiOCl2 leached and purified from low-grade ilmenite mineral

Hydrometallurgy ◽

10.1016/j.hydromet.2013.08.002 ◽

2013 ◽

Vol 139 ◽

pp. 88-94 ◽

Cited By ~ 11

Author(s):

Nasser Y. Mostafa ◽

M.H.H. Mahmoud ◽

Z.K. Heiba

Keyword(s):

Low Grade ◽

Hydrolysis Of

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Insights into the pan-microbiome: skin microbial communities of Chinese individuals differ from other racial groups

Scientific Reports ◽

10.1038/srep11845 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 51

Author(s):

Marcus H. Y. Leung ◽

David Wilkins ◽

Patrick K. H. Lee

Keyword(s):

Community Structure ◽

Species Level ◽

Racial Groups ◽

Opportunistic Pathogens ◽

Single Population ◽

Site Specific ◽

Skin Site ◽

Community Membership ◽

Network Analyses ◽

Host Properties

Abstract Many studies have characterized microbiomes of western individuals. However, studies involving non-westerners are scarce. This study characterizes the skin microbiomes of Chinese individuals. Skin-associated genera, including Propionibacterium, Corynebacterium, Staphylococcus and Enhydrobacter were prevalent. Extensive inter-individual microbiome variations were detected, with core genera present in all individuals constituting a minority of genera detected. Species-level analyses presented dominance of potential opportunistic pathogens in respective genera. Host properties including age, gender and household were associated with variations in community structure. For all sampled sites, skin microbiomes within an individual is more similar than that of different co-habiting individuals, which is in turn more similar than individuals living in different households. Network analyses highlighted general and skin-site specific relationships between genera. Comparison of microbiomes from different population groups revealed race-based clustering explained by community membership (Global R = 0.968) and structure (Global R = 0.589), contributing to enlargement of the skin pan-microbiome. This study provides the foundation for subsequent in-depth characterization and microbial interactive analyses on the skin and other parts of the human body in different racial groups and an appreciation that the human skin pan-microbiome can be much larger than that of a single population.

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Detection of presumptive Bacillus cereus in the Irish dairy farm environment

Irish Journal of Agricultural and Food Research ◽

10.1515/ijafr-2016-0014 ◽

2016 ◽

Vol 55 (2) ◽

pp. 145-151 ◽

Cited By ~ 1

Author(s):

A. O’Connell ◽

E.M. Lawton ◽

D. Leong ◽

P. Cotter ◽

D. Gleeson ◽

...

Keyword(s):

Bacillus Cereus ◽

Ribosomal Rna ◽

Gel Electrophoresis ◽

Tap Water ◽

Dairy Farm ◽

Egg Yolk ◽

Pulsed Field Gel Electrophoresis ◽

Chromogenic Medium ◽

Rrna Sequencing ◽

Bulk Tank

AbstractThe objective of the study was to isolate potentialBacillus cereussensu lato (B.cereus s.l.)from a range of farm environments. Samples of tap water, milking equipment rinse water, milk sediment filter, grass, soil and bulk tank milk were collected from 63 farms. In addition, milk liners were swabbed at the start and the end of milking, and swabs were taken from cows’ teats prior to milking. The samples were plated on mannitol egg yolk polymyxin agar (MYP) and presumptiveB. cereus s.l. colonies were isolated and stored in nutrient broth with 20% glycerol and frozen at -80 °C. These isolates were then plated on chromogenic medium (BACARA) and colonies identified as presumptiveB. cereus s.l. on this medium were subjected to 16S ribosomal RNA (rRNA) sequencing. Of the 507 isolates presumed to beB. cereus s.l. on the basis of growth on MYP, only 177 showed growth typical ofB. cereus s.l. on BACARA agar. The use of 16S rRNA sequencing to identify isolates that grew on BACARA confirmed that the majority of isolates belonged toB. cereus s.l. A total of 81 of the 98 isolates sequenced were tentatively identified as presumptiveB. cereus s.l. Pulsed-field gel electrophoresis was carried out on milk and soil isolates from seven farms that were identified as having presumptiveB. cereus s.l. No pulsotype was shared by isolates from soil and milk on the same farm. PresumptiveB. cereus s.l. was widely distributed within the dairy farm environment.

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Producing hydrogen in an aqueous NaCl solution by the hydrolysis of metallic couples of low-grade magnesium scrap and noble metal net

International Journal of Hydrogen Energy ◽

10.1016/j.ijhydene.2008.11.097 ◽

2009 ◽

Vol 34 (4) ◽

pp. 1677-1687 ◽

Cited By ~ 32

Author(s):

Jun-Yen Uan ◽

Meng-Chang Lin ◽

Chi-Yuan Cho ◽

Kun-Ta Liu ◽

Heng-I. Lin

Keyword(s):

Noble Metal ◽

Low Grade ◽

Nacl Solution ◽

Hydrolysis Of

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Persistence of methylated bases in ribonucleic acid of syrian golden hamster liver after administration of dimethylnitrosamine

Biochemical Journal ◽

10.1042/bj1770967 ◽

1979 ◽

Vol 177 (3) ◽

pp. 967-973 ◽

Cited By ~ 8

Author(s):

G P Margison ◽

J M Margison ◽

R Montesano

Keyword(s):

Ribosomal Rna ◽

Golden Hamster ◽

Excision Repair ◽

Experimental System ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Syrian Golden Hamster ◽

Low Doses ◽

Enzymic Hydrolysis ◽

Hydrolysis Of

1. Syrian golden hamster liver ribosomal RNA was isolated up to 96 h after administration of [14C]dimethylnitrosamine at 25 mg/kg or 2.5 mg/kg body weight. 2. The chemical alkyation products, 7-methylguanine, 3-methylcytosine, O6-methylguanosine and 1-methyladenosine, were measured after acidic or enzymic hydrolysis of the RNA to bases or mononucleosides followed by ion-exchange chromatography. 3. Between 7 and 96 h, the relative amounts of alkylation products did not change with time even though the absolute amounts fell by approx. 80% and 51% after the high and low doses respectively. 4. The results suggest that base specific excision repair does not exist for RNA alkylation products in this experimental system.

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Molecular Identification of Nontuberculous Mycobacteria in Humans in Zimbabwe Using 16S Ribosequencing

The Open Microbiology Journal ◽

10.2174/1874285801610010113 ◽

2016 ◽

Vol 10 (1) ◽

pp. 113-123 ◽

Cited By ~ 7

Author(s):

Nyasha Chin’ombe ◽

Boniface Muzividzi ◽

Ellen Munemo ◽

Pasipanodya Nziramasanga

Keyword(s):

Ribosomal Rna ◽

Nontuberculous Mycobacteria ◽

Reference Laboratory ◽

Opportunistic Pathogens ◽

Chain Reaction ◽

Future Studies ◽

True Diversity ◽

National Tuberculosis ◽

Mycobacterium Avium Intracellulare ◽

Polymerase Chain

Background:Several nontuberculous mycobacteria (NTM) were previously isolated from diverse environments such as water, soil, sewage, food and animals. Some of these NTM are now known to be opportunistic pathogens of humans.Objective:The main purpose of the study was to identify NTM isolates stored at the National Microbiology Reference Laboratory (NMRL) and were previously isolated from humans during a national tuberculosis (TB) survey.Methods:Pure NTM cultures already isolated from human sputum samples during the national TB survey were retrieved from the NMRL and used for this study. DNA was extracted from the samples and 16S ribosomal RNA gene amplified by polymerase chain reaction. The amplicons were sequenced and bioinformatics tools were used to identify the NTM species.Results:Out of total of 963 NTM isolates stored at the NMRL, 81 were retrieved for speciation. Forty isolates (49.4%) were found to belong toMycobacterium avium-intracellularecomplex (MAC) species. The other 41 isolates (50.6%) were identified asM. lentiflavum(6.2%),M. terraecomplex (4.9%),M. paraense(4.9%),M. kansasii(3.7%),M. moriokaense(3.7%),M. asiaticum(2.5%),M. novocastrense(2.5%),M. brasiliensis(2.5%),M. elephantis(2.5%),M. paraffinicum(1.2%),M. bohemicum(1.2%),M. manitobense(1.2%),M. intermedium(1.2%),M. tuberculosiscomplex (1.2%),M. parakoreense(1.2%),M. florentinum(1.2%),M. litorale(1.2%),M. fluoranthenivorans(1.2%),M. sherrisii(1.2%),M. fortuitum(1.2%) andM septicum(1.2%). Two isolates (2.5%) could not be identified, but were closely related toM. montefiorenseandM. phleirespectively. Interestingly, the MAC species were the commonest NTM during the survey.Conclusion:The study emphasizes the importance of identifying species of NTM in Zimbabwe. Future studies need to ascertain their true diversity and clinical relevance.

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In Silico and Experimental Evaluation of Primer Sets for Species-Level Resolution of the Vaginal Microbiota Using 16S Ribosomal RNA Gene Sequencing

The Journal of Infectious Diseases ◽

10.1093/infdis/jiy508 ◽

2018 ◽

Vol 219 (2) ◽

pp. 305-314 ◽

Cited By ~ 10

Author(s):

William J Van Der Pol ◽

Ranjit Kumar ◽

Casey D Morrow ◽

Eugene E Blanchard ◽

Christopher M Taylor ◽

...

Keyword(s):

Ribosomal Rna ◽

Experimental Evaluation ◽

In Silico ◽

Gene Sequencing ◽

Species Level ◽

Ribosomal Rna Gene ◽

Operational Taxonomic Units ◽

16S Ribosomal Rna Gene ◽

Primer Sets ◽

Level Identification

V4 sequence reads clustered at 99% identity and assigned to operational taxonomic units using the 99% clustered, extended Greengenes database provided optimal species-level identification of vaginal bacteria. This method provided results similar to those obtained with DADA2 and/or using the SILVA database.

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