Effects of Licorice (Glycyrrhiza glabra) on testicular oxidative stress in sleep-deprived male Wistar rats

Background: Sleep deprivation is a public health problem that causes oxidative stress injury. Research evidence agrees that oxidative stress serves as an underlying factor in many chronic debilitating diseases. This study investigated the effect of aqueous Licorice extract (Glycyrrhiza glabra) a medicinal plant with known antioxidant activity on testicular oxidative stress parameters. Methods Twenty-five male Wistar rats were randomly allocated to and administered one of the following treatment regimens daily for five days: CONTROL – Distilled water, Sleep – Deprived (SD) – Distilled water, Sleep Deprived +Sleep Recovery (SD+SR) – Distilled Water, SD+LICORICE (SD+LICORICE) – 150 mg/kg bodyweight of Licorice and Sleep – Deprivation + Sleep-Recovery + Licorice (SD+SR+LICORICE) – 150 mg/kg bodyweight of Licorice. The rats in Sleep – Recovery groups were allowed to sleep in their cages after sleep deprivation protocol for 5 days each treatment regimen had ad libitum access to standard rat chow. After the experiment, the rats were sacrificed and blood was collected. Serum cortisol and testosterone were taken alongside testicular Glutathione, Catalase, and Malondialdehyde. Results Sleep deprivation significantly raised cortisol level and decreased testosterone levels both of which were reversed by licorice administration. Significant reduction in Malondialdehyde (MDA) in rats treated with licorice with a simultaneous increase in both GSH and CAT was also observed. Conclusions The antioxidant activity of licorice (Glycyrrhiza glabra) aqueous extract on the testis of rats exposed to oxidative stress suggests the potential of using this traditional medicinal plant in preventing oxidative injury caused by sleep deprivation.

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Analysing the Hypolipidemic Activities of the Tea Extracts of Moringa oleifera, Glycyrrhiza glabra and their Blend at Different Concentrations, Orally Induced on Adult Male Wistar Rats.

International Journal of Pharmacology Phytochemistry and Ethnomedicine ◽

10.18052/www.scipress.com/ijppe.3.76 ◽

2016 ◽

Vol 3 ◽

pp. 76-83

Author(s):

Olanrewaju Roland Akinseye

Keyword(s):

Wistar Rats ◽

Moringa Oleifera ◽

Glycyrrhiza Glabra ◽

Atherogenic Index ◽

Simultaneous Increase ◽

Hypolipidemic Effect ◽

Aqueous Extracts ◽

Water Control ◽

Beneficial Effects ◽

Male Wistar Rats

Herbal teas ofMoringa oleifera(Moringa) andGlycyrrhiza glabra(Licorice)were known to have many beneficial effects, their possible hypolipidemic effects were investigated in this paper. Albino wistar rats were fed with aqueous extracts ofM. oleifera,G. glabraand their blend teas(10, 30 and 50 mg/kg.BW/ml). The teas were found to lower the serum cholesterol, triglycerides, VLDL, LDL, and atherogenic index at their higher concentration, but were found to slightly increase the HDL as compared to the corresponding normal healthy rats fed with clean water (control). The phytochemicals screening of the teas aqueous extract were also investigated, the chemicals (tannins, saponins, flavonoids, terpenoids and glycosides) which were known to exhibit cholesterol lowering effect by inhibiting its absorption and simultaneous increase its excretion were found to be present in the teas extract. Thus, the study demonstrates and validates thatM.oleifera,G. glabraand their blend possess hypolipidemic effect at higher concentration.

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Investigation of the role of α-lipoic acid on fatty acids profile, some minerals (zinc, copper, iron) and antioxidant activity against aluminum-induced oxidative stress in the liver of male rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2015-0160 ◽

2017 ◽

Vol 28 (4) ◽

Cited By ~ 4

Author(s):

Zafer Sahin ◽

Ahmet Ozkaya ◽

Okkes Yilmaz ◽

Abdurrauf Yuce ◽

Mehmet Gunes

Keyword(s):

Oxidative Stress ◽

Fatty Acids ◽

Antioxidant Activity ◽

Lipoic Acid ◽

Wistar Rats ◽

Male Rats ◽

Fatty Acids Profile ◽

Male Wistar Rats ◽

Aluminum Accumulation

AbstractBackground:We have investigated the effects of α-lipoic acid (LA), a powerful antioxidant, on the fatty acid (FA) profiles, aluminum accumulation, antioxidant activity and some minerals such as zinc, copper and iron against aluminum chloride (AlClMethods:Twenty-eight male Wistar rats were divided into four groups as control, LA, AlClResults:AlClConclusions:These results indicate that AlCl

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The mitigating effect of Ananas comosus on aluminum-induced oxidative stress on the testes of adult male Wistar rats

The Journal of Basic and Applied Zoology ◽

10.1186/s41936-021-00210-5 ◽

2021 ◽

Vol 82 (1) ◽

Author(s):

Bankole J. Leko ◽

Solomon T. Olawuyi ◽

Lawrence U. Okon

Keyword(s):

Oxidative Stress ◽

Aluminum Chloride ◽

Wistar Rats ◽

Negative Control Group ◽

Negative Control ◽

Ananas Comosus ◽

Control Group ◽

Distilled Water ◽

Pineapple Juice ◽

Male Wistar Rats

Abstract Background The mitigating effect of Ananas comosus (pineapple juice) extract on an aluminum-induced testicular toxicity in male Wistar rats was examined in this study. Thirty healthy adult male Wistar rats with an average weight of 200 g were grouped into six groups; distilled water and 1 ml of pineapple juice extracts were administered to the control and treated animals respectively for 3 weeks. The control group was given rat pellets and distilled water. Negative control was given 100mg/kg/d Aluminum Chloride and pellets; Group 1 was given 100mg/kg/d and 1ml of pineapple juice in distilled water orally; Group 2 was given 100mg/kg/d Aluminum Chloride and 1.5 ml of in distilled water orally; group 3 was given 100 mg/kg/day aluminum chloride and 2 ml of pineapple juice in distilled water orally; group 4 was given 100 mg/kg/day aluminum chloride and 2.5 ml of pineapple juice in distilled water orally. Testicular histology, semen parameters, and testosterone were assessed. Results This study showed a significant (P < 0.05) decrease in testicular volume, motile sperm count, concentration, total count, progressive evaluation, and morphology in the negative control group relative to the normal control and extract control groups. In the groups co-treated with aluminum chloride and Ananas comosus extract, there was improvement in sperm volume, motility, total count, progressive assessment, and morphology. There was also a statistical decrease (P < 0.05) in testosterone hormone in the negative control group, but there was an increase in the aluminum chloride and Ananas comosus extract co-treated groups. Similarly, in co-treated aluminum chloride and Ananas comosus extract, the degenerative seminiferous tubule histoarchitecture due to aluminum chloride in the negative control group was enhanced. Conclusion Based on this current study, it was evident that aluminum chloride induced oxidative stress and retarded reproduction in males whereas Ananas comosus mitigated reproduction in males by improving sperm parameters and microarchitecture of the testes.

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Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2020-0178 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Taye J. Lasisi ◽

Shehu-Tijani T. Shittu ◽

Jude I. Abeje ◽

Kehinde J. Ogunremi ◽

Seyyid A. Shittu

Keyword(s):

Oxidative Stress ◽

Sleep Deprivation ◽

Wistar Rats ◽

Paradoxical Sleep ◽

Salivary Secretion ◽

Control Group ◽

Paradoxical Sleep Deprivation ◽

Sod Activity ◽

Submandibular Glands ◽

Male Wistar Rats

Abstract Objectives Paradoxical sleep deprivation has been associated with impaired salivary secretion in rats. However, the mechanism that underlies this is not known. Therefore, this study assessed salivary and serum oxidative stress levels following paradoxical sleep deprivation in rats. Methods Twenty-one male Wistar rats randomly divided into three groups of seven rats each as; Control (C); partial sleep-deprived (PSD); and total sleep-deprived (TSD) were used. Malondialdehyde (MDA) concentration, Superoxide dismutase (SOD), and catalase activities were evaluated in saliva, serum, and submandibular glands after seven days of sleep deprivation. Data were expressed as mean ± standard error of the mean and analyzed using one-way ANOVA, Tukey HSD post hoc, and Pearson’s correlation tests. Results Serum MDA levels were significantly higher in both the TSD and PSD groups compared to the control group whereas only the TSD group showed higher submandibular MDA levels compared to the PSD group and the control group. Submandibular SOD activity was significantly lower in both the TSD and PSD groups compared to the control group. Serum catalase activity was significantly lower in the TSD group only compared to the control group. Conclusions These results have demonstrated for the first time that paradoxical sleep deprivation was associated with changes in the oxidant/antioxidant defense system in the submandibular salivary glands of male Wistar rats which may contribute to impairment in salivary secretion.

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Sleep Recovery Restored Neuroglobin Immunoreactivity in Rat LDTg-PPTg Nuclei

Sleep Disorders ◽

10.1155/2020/8353854 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Montserrat Melgarejo-Gutiérrez ◽

Fabio García-García ◽

Gerardo Hernández-Márquez ◽

Consuelo Morgado-Valle ◽

Mario Eduardo Acosta-Hernández ◽

...

Keyword(s):

Oxidative Stress ◽

Nervous System ◽

Sleep Deprivation ◽

Wistar Rats ◽

Cell Damage ◽

Brain Areas ◽

Male Wistar Rats ◽

Laterodorsal Tegmentum ◽

Handling Method ◽

And Oxidative Stress

Neuroglobin (Ngb) is a protein member of the globin family, expressed mainly in the central and peripheral nervous system. It is involved in the transport of oxygen in response to hypoxic/ischemic and oxidative stress-related insults. We recently showed that sleep deprivation reduces the number of Ngb-positive cells in brain areas related to sleep. However, it is poorly understood whether Ngb expression correlates with sleep occurrence. Here, we aimed to study if sleep recovery produced by 24 h of sleep deprivation restores the number of Ngb-positive cells in the pedunculopontine tegmentum (PPTg) and laterodorsal tegmentum (LDTg), brain areas related to sleep-wake regulation. Male Wistar rats were sleep-deprived for 24 h using the gentle handling method. After sleep deprivation, rats were allowed a sleep recovery for three or six hours. After sleep recovery, rats were euthanized, and their brains processed for Ngb immunohistochemistry. We found that a 3 h sleep recovery is enough to restore the number of Ngb-positive cells in all the analyzed areas. A similar result was observed after a 6 h sleep recovery. These results suggest that Ngb expression is sleep dependent. We suggest that Ngb expression is involved in preventing cell damage due to prolonged wakefulness.

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