Antibacterial Efficacy of Pastes Against E Faecalis in Primary Root Dentin: A Confocal Microscope Study

2015 ◽  
Vol 39 (3) ◽  
pp. 247-254 ◽  
Author(s):  
R Verma ◽  
DS Sharma ◽  
AK Pathak
Keyword(s):  
Enterococcus Faecalis ◽  
Primary Teeth ◽  
Univariate Analysis ◽  
Primary Root ◽  
Pediatric Dentistry ◽  
Antimicrobial Efficacy ◽  
Bacterial Suspension ◽  
Dead Cell ◽  
Dentinal Tubules ◽  
Significant Difference

Objectives. Management of abscessed primary teeth often present endodontic failure owing to questioned efficiency of dressings or obturating pastes to eliminate Enterococcus faecalis, a resistant bacterium, residing in depth of dentinal tubules. The present study evaluates the antimicrobial efficacy of two antibacterial and two obturating pastes in dentinal tubules of primary teeth infected with Enterococcus faecalis using viability stain and confocal laser scanning microscope (CLSM). Study design. Total 28 samples were prepared.Four groups with 6 samples each were made according to antibacterial pastes i.e. 1% or 2%Chlorhexidine (CHX) + calcium hydroxide (CH), CH + iodoform (Metapex) and Zinc Oxide Eugenol (ZOE). Dentinal tubules from the root canal side were infected with E. faecalis by centrifugation of the bacterial suspension. Two specimens from each group were subjected to 1, 7 and 15 days antibacterial pastes exposure. Viability staining followed by CLSM were used to quantitatively analyze the dead cell count directly inside dentin. Result: Univariate analysis showed that all medicaments were significantly effective (p<.05). Kruskal wallis ANOVA test did not show significant difference among four medicaments at day 1 while it was significantly different at day 7 & 15. Paired sample student’s t-test revealed significant difference in efficacy between 1& 15 days for 1%CHX+CH; between 1&15,and 7&15 days; between all days for ZOE. Ranking of antimicrobial efficacy of tested medicament was (most effective to the least): 1%CHX+CH(15)> ZOE(15)> Metapex(15)> 2%CHX+CH(15)> 2%CHX+CH(7)> 2%CHX+CH(1)> 1%CHX+CH(7)> 2%CHX+CH(15)> Metapex(1)> ZOE(1)> ZOE(7). Conclusions: All medicaments were effective against E. faecalis in dentine of primary teeth and their efficacy increased with longer contact with 1%CHX+CH being most effective at day 15. Inclusion of 1% CHX in dressings or obturating pastes might minimize the endodontic relapse and maximize the tooth retention in functional state in pediatric dentistry.

An in Vivo Comparison of Antimicrobial Efficacy of Sodium Hypochlorite and Biopure MTAD™ against Enterococcus Faecalis in Primary Teeth: A qPCR Study

2014 ◽  
Vol 39 (1) ◽  
pp. 30-34 ◽  
Author(s):  
SG Tulsani ◽  
N Chikkanarasaiah ◽  
S Bethur
Keyword(s):  
Root Canal ◽  
Primary Teeth ◽  
Antimicrobial Efficacy ◽  
Permanent Teeth ◽  
Control Group ◽  
Statistical Significant Difference ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Root Canal Irrigant

Objectives: Biopure MTAD™, a new root canal irrigant has shown promising results against the most common resistant microorganism, E. faecalis, in permanent teeth. However, there is lack of studies comparing its antimicrobial effectiveness with NaOCl in primary teeth. The purpose of this study was to compare the in vivo antimicrobial efficacy of NaOCl 2.5% and Biopure MTAD™ against E. faecalis in primary teeth. Study design: Forty non vital single rooted primary maxillary anterior teeth of children aged 4-8 years, were irrigated either with NaOCl 2.5% (n=15), Biopure MTAD™ (n=15) and 0.9% Saline (n=10, control group). Paper point samples were collected at baseline (S1) and after chemomechanical preparation (S2) during the pulpectomy procedure. The presence of E. faecalis in S1 & S2 was evaluated using Real time Polymerase Chain Reaction. Results: Statistical significant difference was found in the antimicrobial efficacy of NaOCl 2.5 % and BioPure MTAD™ when compared to saline (p>0.05). However, no statistical significant difference was found between the efficacies of both the irrigants. Conclusions: NaOCl 2.5% and BioPure MTAD™, both irrigants are equally efficient against E. faecalis in necrotic primary anterior teeth. MTAD is a promising irrigant, however clinical studies are required to establish it as ideal root canal irrigant in clinical practice.

scholarly journals Antimicrobial Efficacy of Fruit Peels Eco-Enzyme against Enterococcus faecalis: An In Vitro Study

2020 ◽  
Vol 17 (14) ◽  
pp. 5107
Author(s):  
Hetal Ashvin Kumar Mavani ◽  
In Meei Tew ◽  
Lishen Wong ◽  
Hsu Zenn Yew ◽  
Alida Mahyuddin ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Antimicrobial Efficacy ◽  
Significant Difference ◽  
Potential Alternative ◽  
The One

Sodium hypochlorite (NaOCl), an effective endodontic irrigant against Enterococcus faecalis (EF), is harmful to periapical tissues. Natural pineapple-orange eco-enzymes (M-EE) and papaya eco-enzyme (P-EE) could be potential alternatives. This study aimed to assess the antimicrobial efficacy of M-EE and P-EE at different concentrations and fermentation periods against EF, compared to 2.5% NaOCl. Fermented M-EE and P-EE (3 and 6 months) at various concentrations were mixed with EF in a 96-well plate incubated for 24 h anaerobically. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of M-EE and P-EE were determined via EF growth observation. EF inhibition was quantitatively measured and compared between different irrigants using the one-way analysis of variance (ANOVA), and different fermentation periods using the independent-samples T-test. M-EE and P-EE showed MIC at 50% and MBC at 100% concentrations. There was no significant difference in antimicrobial effect when comparing M-EE and P-EE at 50% and 100% to 2.5% NaOCl. P-EE at 6 months fermentation exhibited higher EF inhibition compared to 3 months at concentrations of 25% (p = 0.017) and 0.78% (p = 0.009). The antimicrobial properties of M-EE and P-EE, at both 100% and 50% concentrations, are comparable to 2.5% NaOCl. They could therefore be potential alternative endodontic irrigants, but further studies are required.

scholarly journals Comparison of quality of obturation and instrumentation time using hand files and two rotary file systems in primary molars: A single-blinded randomized controlled trial

2017 ◽  
Vol 11 (03) ◽  
pp. 376-379 ◽  
Author(s):  
Lavanya Govindaraju ◽  
Ganesh Jeevanandan ◽  
E. M. G. Subramanian
Keyword(s):  
Primary Teeth ◽  
Controlled Trial ◽  
File Systems ◽  
Pediatric Dentistry ◽  
Primary Molars ◽  
Chi Square ◽  
Intergroup Comparison ◽  
Significant Difference ◽  
Group 2

ABSTRACT Objective: In permanent dentition, different rotary systems are used for canal cleaning and shaping. Rotary instrumentation in pediatric dentistry is an emerging concept. A very few studies have compared the efficiency of rotary instrumentation for canal preparation in primary teeth. Hence, this study was performed to compare the obturation quality and instrumentation time of two rotary files systems – Protaper, Mtwo with hand files in primary molars. Materials and Methods: Forty-five primary mandibular molars were randomly allotted to one of the three groups. Instrumentation was done using K-files in Group 1; Protaper in Group 2; and Mtwo in Group 3. Instrumentation time was recorded. The canal filling quality was assessed as underfill, optimal fill, and overfill. Statistical analysis was done using Chi-square, ANOVA, and post hoc Tukey test. Results: No significant difference was observed in the quality of obturation among three groups. Intergroup comparison of the instrumentation time showed a statistically significant difference between the three groups.Conclusion: The use of rotary instrumentation in primary teeth results in marked reduction in the instrumentation time and improves the quality of obturation.

scholarly journals Antibacterial Efficacy of Different Herbal Based Irrigant Solutions in Deciduous Teeth

2021 ◽  
Author(s):  
Naamatullah Ahmed Mohammed ◽  
Bahar Jaafar Selivany
Keyword(s):  
Green Tea ◽  
Normal Saline ◽  
Primary Teeth ◽  
Primary Root ◽  
Negative Control ◽  
Deciduous Teeth ◽  
Antibacterial Efficacy ◽  
Endodontic Infection ◽  
Significant Difference

Introduction: Endodontic infection requires the successful removal of microorganisms from the root canal system. The most effective irrigant solution is sodium hypochlorite, but possible problems due to its toxicity require the look for new alternatives. Constant increases in antibiotic resistance and side-effects caused by chemical irrigation have shifted research towards the production of herbal alternatives, especially in paediatric dentistry. Aim: To evaluate and compare the antibacterial efficacy of miswak, green tea, chamomile and 0.5% NaOCl as an endodontic irrigants against Enterococcus faecalis in primary root canals. Materials and Methods: The in-vitro study was conducted at the Department of Conservative Dentistry and Department of Microbiology, College of Dentistry, University of Duhok from January 2020 till June 2020. Seventy five freshly extracted intact human mandibular primary molars were decoronated at Cemento-Enamel Junction (CEJ) and distal roots were separated and biomechanically prepared up to F3 Protaper file and stored in normal saline. The specimens were inoculated with Enterococccus faecalis suspension and incubated for 72 hours. Specimens were divided into five groups containing fifteen teeth each (n=15). Freshly prepared alcoholic extracts of miswak, green tea and chamomile were used as an irrigant solution against E.faecalis compared to NaOCl as positive control and normal saline as negative control. Swabs were collected using F3 protaper paper points. The number of colonies was counted in suitable plate under good illumination and manual lens for magnification. Statistical analysis was performed by using Kruskal-Wallis one-way Analysis and Student-Newman-Keuls Method. Statistical Package for the Social Sciences (SPSS) version 26.0 was used. The p-values more than 0.05 were considered as statistically non significant. Results: Green Tea, among the herbal experimental groups, had the most effective antibacterial effect against E.faecalis. No significant statistical difference was detected between green tea (p=0.272) and NaOCl; however, there was significant difference between miswak, chamomile and NaOCl as well as between the rest herbal experimental groups and normal saline. Conclusion: NaOCl remains the gold standard as irrigant in primary teeth. Green tea extract may help in reducing E.faecalis inside the canals of primary teeth. Good efficacy against E.faecalis was also shown by Miswak and chamomile however the results obtained were not significant when compared with NaOCl.

scholarly journals Parental acceptability of coloured compomer as a restorative material in primary teeth of children - A randomized split mouth clinical trial

2020 ◽  
Vol 11 (4) ◽  
pp. 6845-6849
Author(s):  
Neethu Ann Preethy ◽  
Sujatha Somasundaram
Keyword(s):  
Primary Teeth ◽  
Pediatric Dentistry ◽  
Parental Satisfaction ◽  
Control Group ◽  
Restorative Material ◽  
Significant Difference ◽  
The Aesthetic ◽  
Parental Acceptability ◽  
Experimental Group ◽  
Standard Operating Protocol

Coloured compomer materials have been introduced in Pediatric Dentistry and gained popularity as it develops a positive behaviour in the child and makes further treatment stress-free. However, parental satisfaction regarding the appearance of coloured compomer is questionable. This study aimed to compare the parental acceptability of coloured compomer compared to conventional compomer for the restoration of primary teeth in children. A total of sixty sites, divided into two groups, with thirty subjects in each group using split-mouth design were chosen amongst patients aged between 5 to 10 years reporting to the Department of Pedodontics and Preventive Dentistry. In the control group, conventional compomer(Material A) were placed, similarly coloured compomers(Material B) were placed in the experimental group under the standard operating protocol. Parental preference was noted by asking them to tick or point to the appropriate box labelled Material A and Material B, and the results were evaluated by statistical analysis using SPSS version 21.0. A statistically significant difference was found in parents in preference of conventional tooth coloured compomer as the restorative material. (p<0.05)Thus, the preference of parents regarding the placement of coloured compomer in primary teeth is comparatively low. Though the coloured compomer material positively influences the behaviour and treatment outcome in children, the parental satisfaction is found to be comparatively low as parents feel that it lacks the aesthetic appeal when compared to the conventional tooth-coloured restorations.

Comparison of Various Root Canal Filling Materials and Obturation Techniques for Primary Teeth Using Microcomputed Tomography

2021 ◽  
pp. 232020682110361
Author(s):  
Gulce Ozturk ◽  
Mustafa Aydinbelge ◽  
Husniye Gumus
Keyword(s):  
Local Anesthesia ◽  
Root Canal ◽  
Primary Teeth ◽  
Primary Root ◽  
Microcomputed Tomography ◽  
The Other ◽  
Micro Ct ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Significant Difference

Aim: The aim of this study was to compare the effectiveness of different filling materials and obturation techniques for root canal obturation in primary teeth. Materials and Methods: Eighty maxillary primary incisors were included in this in vitro study. After biomechanical preparation, all samples were scanned using microcomputed tomography (micro-CT) and root canal volumes were calculated. Samples were grouped according to filling materials and obturation techniques, with ten samples in each group. The samples were filled with zinc oxide eugenol (ZOE) and Vitapex using four different obturation techniques (local anesthesia syringe, lentulo spiral, pastinject, and lentulo spiral–ultrasonic activation). After filling, samples were scanned a second time using micro-CT and root canal volumes were calculated. The statistical analysis was performed using SPSS version 24.0. Data were analyzed by Kruskal–Wallis and Mann–Whitney U tests. Results: When the ZOE and Vitapex were compared, it was observed that there were no significant differences between their postobturated volumes, percentage obturated volume (POV), internal void (IV), and percentage of internal void volume (PIV) ( P > .05). The local anesthesia syringe group had a significantly lower postobturated volume and POV, and a significantly higher IV and PIV for both filling materials than that by the other groups ( P < .05). There was no significant difference between the other obturation techniques ( P > .05). For the local anesthesia syringe group, postobturated volume and POV were significantly higher and IV and PIV were significantly lower with Vitapex than with ZOE ( P < .05). There was no significant difference between the other obturation techniques filled either with ZOE or Vitapex ( P > .05). Conclusion: Except for the syringe technique, no difference was observed between the ZOE and Vitapex materials. The syringe technique used in the primary root canal filling was less effective than the other techniques, which had similar efficacy, and in this technique, more effective canal filling was achieved with Vitapex.

scholarly journals GARLIC EXTRACT EFFICACY AGAINST THE VIABILITY OF ENTEROCOCCUS FAECALIS (IN VITRO)

2019 ◽  
pp. 194-197
Author(s):  
ANDRIANI OCTAVIA ◽  
SARWORINI BAGIO BUDIARDJO ◽  
IKE SITI INDIARTI ◽  
EVA FAUZIAH ◽  
MARGARETHA SUHARSINI ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Primary Root ◽  
Anaerobic Bacteria ◽  
Garlic Extract ◽  
Gram Positive ◽  
Dentinal Tubules ◽  
Significance Level ◽  
Post Hoc

Objective: Enterococcus faecalis, Gram-positive facultative anaerobe capable of invading the dentinal tubules, is resistant to several irrigating solutionsand intracanal medications. Motivated by the antibacterial effect exhibited by garlic extract against a wide variety of Gram-positive and Gram-negativebacteria, as well as anaerobic bacteria such as Lactobacillus and E. faecalis, we aimed in this study to analyze the efficacy of garlic extract against theviability of E. faecalis from clinical isolates of nonvital primary root canals.Methods: We used MTT assay to determine the viability of E. faecalis after exposure to increasing concentrations of garlic extract (10%, 25%, 50%,and 100%) and chlorhexidine (CHX) 2% as a positive control.Results: We performed the analysis using the Kruskal–Wallis test and the post hoc test for multiple comparisons at a 0.05 significance level. At allconcentrations, garlic extracts decreased the viability of E. faecalis. We found no significant differences between the viability values for 25%, 50%,and 100% garlic extracts (p>0.05); however, they were significantly different from the 10% extract and CHX 2% (p<0.05), both with a lower viabilityvalues.Conclusion: Our results showed that garlic extract was effective in decreasing the viability of E. faecalis.

scholarly journals Comparative Evaluation of Antimicrobial Efficacy of Neem, Miswak, Propolis, and Sodium Hypochlorite against Enterococcus faecalis using EndoVac

2017 ◽  
Vol 7 (2) ◽  
pp. 60-65
Author(s):  
Priya Daga ◽  
Hemant Asrani ◽  
Shanin Farista ◽  
Praveen Mishra
Keyword(s):  
Enterococcus Faecalis ◽  
Optical Density ◽  
Sodium Hypochlorite ◽  
Comparative Evaluation ◽  
Irrigation System ◽  
Statistical Significance ◽  
Antimicrobial Efficacy ◽  
P Value ◽  
Group I ◽  
Significant Difference

ABSTRACT Aim To compare the antimicrobial efficacy of herbal irrigants neem, miswak, propolis with sodium hypochlorite using conventional needle irrigation and EndoVac irrigation system against Enterococcus faecalis. Materials and methods A total of 120 extracted single-rooted mandibular premolar teeth were infected for 21 days with E. faecalis after instrumentation with ProTaper system. Before irrigation procedure, dentinal shavings were collected in 1 mL of sterile broth and incubated. The optical density of each broth was measured using digital colorimeter and initial readings were recorded. Samples were then divided into four groups of 30 teeth each – Group I: Sodium hypochlorite irrigation, group II: Neem irrigation, group III: Miswak irrigation, group IV: propolis irrigation. Each group was further divided into two subgroups – (a) EndoVac irrigation (b) conventional needle irrigation. After irrigation, dentinal shavings were collected and optical density recorded. The values were analyzed statistically with Student's t test and analysis of variance followed by Tukey's honest significant difference test; p-value < 0.05 was considered to be statistically significant. Results The postirrigation optical densities in all the groups were significantly lower than preirrigation values. Sodium hypochlorite demonstrated better antimicrobial efficacy followed by propolis, neem, and miswak. Differences in optical density values for all irrigants are higher in EndoVac (p < 0.0001) compared with needle (p = 0.0009) group, but it failed to reach statistical significance. Conclusion Sodium hypochlorite proved to be a better irrigant followed by propolis, neem, and miswak. EndoVac irrigation system was more effective for elimination of E. faecalis than needle irrigation group. How to cite this article Daga P, Asrani H, Farista S, Mishra P. Comparative Evaluation of Antimicrobial Efficacy of Neem, Miswak, Propolis, and Sodium Hypochlorite against Enterococcus faecalis using EndoVac. Int J Prosthodont Restor Dent 2017;7(2):60-65.

scholarly journals Cohort study of the overall survival of patients with pancreatic cancer in a hospital of specialties of Quito-Ecuador in the period 2007–2017

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Andrés Moreno Roca ◽  
Luciana Armijos Acurio ◽  
Ruth Jimbo Sotomayor ◽  
Carlos Céspedes Rivadeneira ◽  
Carlos Rosero Reyes ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cohort Study ◽  
Survival Time ◽  
Univariate Analysis ◽  
Rank Test ◽  
Kaplan Meier ◽  
Significant Difference ◽  
Icd 10 ◽  
The Difference ◽  
Clinical Records

Abstract Objectives Pancreatic cancers in most patients in Ecuador are diagnosed at an advanced stage of the disease, which is associated with lower survival. To determine the characteristics and global survival of pancreatic cancer patients in a social security hospital in Ecuador between 2007 and 2017. Methods A retrospective cohort study and a survival analysis were performed using all the available data in the electronic clinical records of patients with a diagnosis of pancreatic cancer in a Hospital of Specialties of Quito-Ecuador between 2007 and 2017. The included patients were those coded according to the ICD 10 between C25.0 and C25.9. Our univariate analysis calculated frequencies, measures of central tendency and dispersion. Through the Kaplan-Meier method we estimated the median time of survival and analyzed the difference in survival time among the different categories of our included variables. These differences were shown through the log rank test. Results A total of 357 patients diagnosed with pancreatic cancer between 2007 and 2017 were included in the study. More than two-thirds (69.9%) of the patients were diagnosed in late stages of the disease. The median survival time for all patients was of 4 months (P25: 2, P75: 8). Conclusions The statistically significant difference of survival time between types of treatment is the most relevant finding in this study, when comparing to all other types of treatments.

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