scholarly journals Comparative Study of Periapical Radiographic Techniques with Apex Locator for Endodontic Working Length Estimation: An ex vivo Study

2011 ◽  
Vol 12 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Vaishali Parekh ◽  
Chirag Taluja
Keyword(s):  
Comparative Study ◽  
Ex Vivo ◽  
Mean Value ◽  
Length Estimation ◽  
Radiographic Technique ◽  
Working Length ◽  
Significant Difference ◽  
Length Determination ◽  
Ex Vivo Study

ABSTRACT Background Accurate assessment of working length determines the success and prognosis of an endodontic treatment outcome. Various methods are used in determining the working length. Aim Compare the measurements of the apex locator and radiographic technique to determine working length. Methods An ex vivo study was conducted on 20 patients having intact single straight root canal. Only premolars were taken in the study. After doing coronal flaring and irrigation, the radiographic length was determined with an aid of a k-type file and electronic length (EL-Root-ZX) 3rd generation apex locator. After extraction of all the premolars, stereomicroscope was further used to confirm and compare radiographic and electronic apex locator. Results A mean value of 0.5430 ± 0.5741 mm was observed among radiographic techniques. A mean value of 0.4240 ± 0.4587 mm was observed among apex locator techniques. Ttest revealed, no significant difference between the two techniques was observed (p = 0. 615). ‘Two tailed’ t-test revealed intragroup significance both techniques for determining the working length. Conclusion The distance of the apical foramen to the tip of the file: A mean value of 0.4240 ± 0.4587 mm with apex locator technique was observed. Working length of apex locator was more in comparison to radiographic technique. No significant difference between the two techniques was observed (p = 0. 615). Intragroup significance among both techniques for determining the working length was also observed. However, a further study incorporating a larger sample size and utilization of both techniques of working length determination on the same tooth has to be conducted. Clinical significance Combining the apex locator technique and radiographic technique for determination of working length would yield more accurate working length. How to cite this article Parekh V, Taluja C. Comparative Study of Periapical Radiographic Techniques with Apex Locator for Endodontic Working Length Estimation: An ex vivo Study. J Contemp Dent Pract 2011;12(2):131-134.

Electronic determination of root canal working length in primary molar teeth: an in vivo and ex vivo study

2011 ◽  
Vol 44 (5) ◽  
pp. 402-406 ◽  
Author(s):  
A. P. C. A. Beltrame ◽  
T. C. Triches ◽  
N. Sartori ◽  
M. Bolan
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
Primary Molar ◽  
Working Length ◽  
Molar Teeth ◽  
Ex Vivo Study

scholarly journals Correlation of objective image quality and working length measurements in different CBCT machines: An ex vivo study

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
T. G. Wolf ◽  
F. Fischer ◽  
R. K. W. Schulze
Keyword(s):  
Image Quality ◽  
Ex Vivo ◽  
Quality Factors ◽  
Study Objective ◽  
Intra Class Correlation ◽  
Working Length ◽  
Length Determination ◽  
Image Quality Measures ◽  
Operator Reproducibility ◽  
Ex Vivo Study

Abstract To investigate potential correlations between objective CBCT image parameters and accuracy in endodontic working length determination ex vivo. Contrast-to-noise ratio (CNR) and spatial resolution (SR) as fundamental objective image parameters were examined using specific phantoms in seven different CBCT machines. Seven experienced observers were instructed and calibrated. The order of the CBCTs was randomized for each observer and observation. To assess intra-operator reproducibility, the procedure was repeated within six weeks with a randomized order of CBCT images. Multivariate analysis (MANOVA) did not reveal any influence of the combined image quality factors CNR and SR on measurement accuracy. Inter-operator reproducibility as assessed between the two observations was poor, with a mean intra-class correlation (ICC) of 0.48 (95%-CI  0.38, 0.59) for observation No. 1. and 0.40 (95%-CI 0.30, 0.51) for observation No. 2. Intra-operator reproducibility pooled over all observers between both observations was only moderate, with a mean ICC of 0.58 (95%-CI 0.52 to 0.64). Within the limitations of the study, objective image quality measures and exposure parameters seem not to have a significant influence on accuracy in determining endodontic root canal lengths in CBCT scans. The main factor of variance is the observer.

scholarly journals Accuracy of Working Length Determination using NovApex and Root-ZX Apex Locators: An in vitro Study

2017 ◽  
Vol 18 (5) ◽  
pp. 383-385 ◽  
Author(s):  
Vahid Zand ◽  
Saeed Rahimi ◽  
Paria Davoudi ◽  
Abubakr Afshang
Keyword(s):  
Ex Vivo ◽  
In Vitro Study ◽  
High Accuracy ◽  
Human Teeth ◽  
Visual Technique ◽  
Working Length ◽  
Significant Difference ◽  
Length Determination ◽  
Electronic Apex Locator

ABSTRACT Introduction This ex vivo study evaluated the accuracy of the Root-ZX electronic apex locator (EAL) (J. Morita, Tokyo, Japan) and the NovApex (Forum Technologies, Rishon Lezion, Israel) in determining the working length (WL) during endodontic treatment. Materials and methods Forty extracted single-rooted human teeth were selected for this study. The actual WL was measured with visual technique by a size #15 k-file under magnification. Then, the canal lengths were measured electronically with both Root-ZX and NovApex apex locators within ±0.5 and ±1 mm. Mean percentage of data was analyzed between groups using paired t-test, with a statistically significant level of p < 0.05. Results The accuracy of NovApex apex locator was 85% within ±0.5 mm and 92.5% within ±1 mm. The accuracy of Root-ZX apex locator was 70% within ±0.5 mm and 97.5% within ±1 mm. There was no significant difference between the accuracy of the two EALs. Conclusion Both the NovApex and Root-ZX EALs are useful for measuring the WL with high accuracy. Clinical significance Given the importance of accurate WL determination in the success of endodontic treatments, the accuracy of different apex locators should be evaluated. How to cite this article Zand V, Rahimi S, Davoudi P, Afshang A. Accuracy of Working Length Determination using NovApex and Root-ZX Apex Locators: An in vitro Study. J Contemp Dent Pract 2017;18(5):383-385.

scholarly journals Comparison of working length determination using apex locator and manual method - ex vivo study

2016 ◽  
Vol 4 (2) ◽  
pp. 39
Author(s):  
Lucasda Fonseca Roberti Garcia ◽  
JhadyeAlves Carneiro ◽  
FredsonMarcio Acris de Carvalho ◽  
AndreAugusto Franco Marques ◽  
EmilioCarlos Sponchiado Junior ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Manual Method ◽  
Working Length ◽  
Length Determination ◽  
Ex Vivo Study

scholarly journals Comparison of Five different methods of Working length determination: An ex vivo study

Endodontology ◽  
2020 ◽  
Vol 32 (4) ◽  
pp. 187
Author(s):  
PalaniSelvi Kamaraj ◽  
Hemalatha Parandhaman ◽  
Vidhya Raguganesh
Keyword(s):  
Ex Vivo ◽  
Working Length ◽  
Length Determination ◽  
Ex Vivo Study

scholarly journals Influence of embedding media on the accuracy of working length determination by means of apex locator: an ex vivo study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Thomas Gerhard Wolf ◽  
Anna Krauß-Mironjuk ◽  
Richard Johannes Wierichs ◽  
Benjamín Briseño-Marroquín
Keyword(s):  
Ex Vivo ◽  
Human Teeth ◽  
Actual Working ◽  
Diagnostic Unit ◽  
Working Length ◽  
Stereo Microscope ◽  
Length Determination ◽  
Per Se ◽  
Ex Vivo Study ◽  
Electronic Apex Locator

AbstractThe aim of this research was to determine ex vivo the influence on accuracy of five different embedding media, for investigative and educational purposes, and one electronic apex locator. 110 human extracted mature roots of permanent single-rooted human teeth were used. The roots were embedded in alginate, stick sponge, 2% agar–agar and 6% and 12% gelatin. The actual working length to the physiological foramen was determined under a stereo-microscope (16 ×) and the electronic working lengths with the Elements Diagnostic Unit and a K-file ISO 10. The accuracy ranges of the accumulated measurements, when allowing a ± 0.5 mm tolerance, went from 98.2% (6% and 12% gelatin), 93.7% (alginate), 92.8% (2% agar–agar) to 91.7% (sponge). The exact measurements at the physiological foramen ranged from 80.0% (6% gelatin), 76.5% (2% agar–agar), 71.8% (12% gelatin), 68.2% (alginate) to 64.5% (sponge). Although relatively seldom (n = 24), measurements with deviations of more than ± 0.5 mm were also observed; thus, the accuracy of the working length determination results per se can be considered as clinically acceptable. The results of this research allow a recommendation of the investigated embedding media for electronic working length determination models for educational and research purposes in endodontics.

Detection of Circulating Tissue Factor and Factor VII in a Normal Population

1996 ◽  
Vol 75 (05) ◽  
pp. 772-777 ◽  
Author(s):  
Sybille Albrecht ◽  
Matthias Kotzsch ◽  
Gabriele Siegert ◽  
Thomas Luther ◽  
Heinz Großmann ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Normal Population ◽  
Mean Value ◽  
Factor Vii ◽  
Significant Difference ◽  
Age Dependent ◽  
The Mean ◽  
Highly Correlated ◽  
And Gender

SummaryThe plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation.

Aerosol generation through pars plana vitrectomy

2020 ◽  
pp. bjophthalmol-2020-317214
Author(s):  
Hasan Naveed ◽  
Fong May Chew ◽  
Hanbin Lee ◽  
Edward Hughes ◽  
Mayank A Nanavaty
Keyword(s):  
Pars Plana Vitrectomy ◽  
Ex Vivo ◽  
Dynamic Changes ◽  
Aerosol Generation ◽  
Significant Difference ◽  
Optical Particle Counter ◽  
Pars Plana ◽  
23 Gauge ◽  
Set Up ◽  
Ex Vivo Study

PurposeTo assess whether pars plana vitrectomy (PPV) is an aerosol-generating procedure (AGP) in an ex vivo experimental model.MethodsIn this ex vivo study on 10 porcine eyes, optical particle counter was used to measure particles ≤10 μm using cumulative mode in the six in-built channels: 0.3 μm, 0.5 μm, 1 μm, 2.5 μm, 5 μm and 10 μm aerosols during PPV. Two parts of the study were as follows: (1) to assess the pre-experimental baseline aerosol count in the theatre environment where there are dynamic changes in temperature and humidity and (2) to measure aerosol generation with 23-gauge and 25-gauge set-up. For each porcine eye, five measurements were taken for each consecutive step in the experiment including pre-PPV, during PPV, fluid–air exchange (FAX) and venting using a flute with 23-gauge set-up and a chimney with 25-gauge set-up. Therefore, a total of 200 measurements were recorded.ResultsWith 23-gauge and 25-gauge PPV, there was no significant difference in aerosol generation in all six channels comparing pre-PPV versus PPV or pre-PPV versus FAX. Venting using flute with 23-gauge PPV showed significant reduction of aerosol ≤1 μm. Air venting using chimney with 25-gauge set-up showed no significant difference in aerosol of ≤1 μm. For cumulative aerosol counts of all particles measuring ≤5 μm, compared with pre-PPV, PPV or FAX, flute venting in 23-gauge set-up showed significant reduction unlike the same comparison for chimney venting in 25-gauge set-up.ConclusionPPV and its associate steps do not generate aerosols ≤10 μm with 23-gauge and 25-gauge set-ups.

scholarly journals Root canal curvature as a prognostic factor influencing the diagnostic accuracy of radiographic working length determination and postoperative canal axis modification: an in vitro comparative study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bestoon Mohammed Faraj
Keyword(s):  
Root Canal ◽  
Radiographic Images ◽  
The Real ◽  
Root Canals ◽  
Length Estimation ◽  
Working Length ◽  
Length Determination ◽  
Case Difficulty Assessment ◽  
The Impact

Abstract Background Radiographic analysis of tooth morphology is mandatory for accurate calibration of the degree of canal curvature angle and radiographic working length to its real dimensions in case difficulty assessment protocols. This study aimed to determine the impact of the degree of root canal curvature angle on maintaining the real working length and the original canal axis of prepared root canals using a reciprocating rotary instrumentation technique. Methods Radiographic image analysis was performed on 60 extracted single-rooted human premolar teeth with a moderate canal curvature (10°–25°) and severe canal curvature (26°–70°). Working length and longitudinal canal axis were determined using cone-beam computed tomography (CBCT) and digital periapical radiography. The real canal length was determined by subtracting 0.5 mm from the actual canal length. Root canals were prepared using the WaveOne Gold reciprocating file (Dentsply Maillefer, Ballaigues, Switzerland). Results There was no significant relation of the degree of canal curvature angle to the accuracy of radiographic working length estimated on CBCT and digital periapical radiographic techniques (P > 0.05). Postinstrumentation changes in the original canal axis between moderate and severe canal curvature angles, assessed on CBCT and periapical digital radiographic images were statistically non-significant (P > 0.05). Conclusions A standardized digital periapical radiographic method performed similarly to the CBCT technique near to its true working length. No significant interaction exists between the diagnostic working length estimation, postoperative root canal axis modification, and the degree of canal curvature angle, using reciprocating rotary instrumentation technique.

scholarly journals Insight into Glutamatergic Involvement in Rewarding Effects of Mephedrone in Rats: In Vivo and Ex Vivo Study

2021 ◽  
Author(s):  
Olga Wronikowska ◽  
Maria Zykubek ◽  
Agnieszka Michalak ◽  
Anna Pankowska ◽  
Paulina Kozioł ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Interdisciplinary Approach ◽  
Nmda Antagonist ◽  
Exchange Chromatography ◽  
Resonance Spectroscopy ◽  
Monoamine Transporters ◽  
Ex Vivo Study ◽  
Insight Into

AbstractMephedrone is a widely used drug of abuse, exerting its effects by interacting with monoamine transporters. Although this mechanism has been widely studied heretofore, little is known about the involvement of glutamatergic transmission in mephedrone effects. In this study, we comprehensively evaluated glutamatergic involvement in rewarding effects of mephedrone using an interdisciplinary approach including (1) behavioural study on effects of memantine (non-selective NMDA antagonist) on expression of mephedrone-induced conditioned place preference (CPP) in rats; (2) evaluation of glutamate concentrations in the hippocampus of rats following 6 days of mephedrone administration, using in vivo magnetic resonance spectroscopy (MRS); and (3) determination of glutamate levels in the hippocampus of rats treated with mephedrone and subjected to MRS, using ion-exchange chromatography. In the presented research, we confirmed priorly reported mephedrone-induced rewarding effects in the CPP paradigm and showed that memantine (5 mg/kg) was able to reverse the expression of this effect. MRS study showed that subchronic mephedrone administration increased glutamate level in the hippocampus when measured in vivo 24 h (5 mg/kg, 10 mg/kg and 20 mg/kg) and 2 weeks (5 mg/kg and 20 mg/kg) after last injection. Ex vivo chromatographic analysis did not show significant changes in hippocampal glutamate concentrations; however, it showed similar results as obtained in the MRS study proving its validity. Taken together, the presented study provides new insight into glutamatergic involvement in rewarding properties of mephedrone.

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