scholarly journals Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study

2013 ◽  
Vol 3 (3) ◽  
pp. 117-120 ◽  
Author(s):  
Hina Sheth ◽  
Uday Kamath ◽  
Sai Ramesh ◽  
Keshav Singla
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Normal Saline ◽  
Diffusion Method ◽  
Saline Control ◽  
Control Group ◽  
Antibacterial Efficacy ◽  
Tea Tree Oil ◽  
Tea Tree

ABSTRACT Background and objectives Among the bacterial species which most often cause endodontic failure, Enterococcus faecalis is the most important. This study compared the antibacterial efficacy of tea tree oil with 3% sodium hypochlorite and 2% chlorhexidine as a root canal irrigant, against E. faecalis Materials and Methods Normal saline, tea tree oil, 3% sodium hypochlorite, 2% chlorhexidine and fresh stains of E. faecalis were used. Four round wells, 4 mm deep and 8 mm diameter, were punched using sterile cork borer in blood agar plates and divided into four groups: group A: normal saline (control), group B: 3% sodium hypochlorite, group C: 2% chlorhexidine and group D: tea tree oil. Agar well diffusion method was performed to compare the antibacterial efficacy of tea tree oil, 3% sodium hypochlorite and 2% chlorhexidine. Results The results were tabulated and statistically analyzed using analysis of variance (ANOVA). Tea tree oil showed comparable inhibition of bacterial growth with sodium hypochlorite and chlorhexidine. No zone of inhibition was shown by normal saline (control). Interpretation and conclusion Tea tree oil showed statistically significant antimicrobial activity against Enterococcus faecalis, which is very much similar to sodium hypochlorite and chlorhexidine. Abbreviations CHX: Chlorhexidine; E. faecalis: Enterococcus faecalis; NaOCl: Sodium hypochlorite. How to cite this article Kamath U, Sheth H, Ramesh S, Singla K. Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study. J Contemp Dent 2013; 3(3):117-120.

Evaluation of the antibacterial effect of tea tree oil on Enterococcus faecalis and biofilm in vitro

2021 ◽  
pp. 114566
Author(s):  
Jianyan Qi ◽  
Min Gong ◽  
Rui Zhang ◽  
Yumeng Song ◽  
Qian Liu ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Tea Tree Oil ◽  
Tea Tree

HERBAL CREAMS OF REISHI EXTRACT AND TEA TREE OIL FOR HIRSUTISM–AN IN VIVO STUDY

2020 ◽  
pp. 106-110
Author(s):  
SANGEETA CHOUDHURY ◽  
BLR MADHAVI
Keyword(s):  
Topical Application ◽  
Hair Growth ◽  
Ethanolic Extract ◽  
Hair Follicles ◽  
In Vivo Studies ◽  
Control Group ◽  
Tea Tree Oil ◽  
Tea Tree

Objective: The aim of this work to formulate, evaluate and compare the effectiveness of herbal creams containing extract of reishi and tea tree oil for treating hirsutism. Methods: Herbal ingredients were authenticated. Cream base was initially formulated. Three formulations of herbal cream were prepared. Reishi ethanolic extract, tea tree oil, and combination of tea tree oil and reishi extract were added to the cream base and formulated cream were named as RHC, THC and RTC respectively. In vitro evaluations on herbal creams were done for the physicochemical characteristics. In vivo studies were carried out on female Swiss Albino mice for the activity against hair growth by topical application of cream to shaved skin. The histological and morphometric evaluation was carried out. Skin irritancy study was conducted. Results: The herbal creams showed desirable physicochemical properties like pH, viscosity and spreadability. Statistical analysis for the length of hair was performed by using one way ANOVA followed by DUNNET’S post hoc test where THC and RTC were found to be significant whereas RHC showed no significant reduction of hair growth compared to control. RTC showed a significant effect at p<0.05 and hair growth reduction was significant for THC at p<0.001 compared to the control group. RTC and THC showed mild to moderate reduction in the size of the hair follicles with a reduction of sebaceous gland size in the histological analysis. Conclusion: Topical application of herbal creams to mice showed that hair growth was fastest in group RHC and was slowest in group THC and intermediate with RTC. It can be concluded that these herbal actives can be used as an effective treatment against hirsutism. Within the study period, tea tree oil was found to be more effective than reishi extract and the combination product. Further formulation studies and in vivo studies need to be carried out on reishi to assess its effectiveness against hirsutism.

scholarly journals Uji Daya Hambat Ekstrak Kulit Langsat (Lansium parasiticum) Terhadap Pertumbuhan Bakteri Enterococcus faecalis Secara In Vitro

2020 ◽  
Vol 8 (2) ◽  
pp. 70-74
Author(s):  
Andhini Ardi ◽  
Dedi Sumantri ◽  
Surma Adnan
Keyword(s):  
Enterococcus Faecalis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Fruit Peel ◽  
Control Group Design ◽  
Inhibition Effect ◽  
Facultative Anaerobic Bacteria ◽  
Peel Extract

Enterococcus faecalis is a bacteria that commonly found in secondary root canal infections. Enterococcus faecalis is a gram-positive, facultative anaerobic bacteria. Langsat (Lansium parasiticum) is a natural ingredient that contains secondary metabolites and has antibacterial ativity. The aim of this study was to examine the inhibition effect of langsat fruit peel extract (Lansium parasiticum) to the growth of Enterococcus faecalis. This was a laboratory experimental study with post-only control group design. Langsat fruit peel extract with 25%, 50%, 75%, and 100% concentration was using disc diffusion method on Mueller-Hinton Agar (MHA) and the inhibition zone was measured with a sliding caliper. The data were analyzed with Kruskal-Wallis test and Mann-Whithney test. The result of this study was langsat fruit peel extract with 25%, 50%, 75% concentration showed a inhibition zone, while at 100% showed no inhibition zone. The Kruskal-Wallis test showed that there are significant differences (p <0.05) between all groups. The conclusion was langsat fruit peel extract (Lansium parasiticum) have weak inhibition effect of the growth of Enterococcus faecalis bacteria. The inhibition zone of concentrations 25% : 2,20 mm;  50%: 0,94 mm; 75%: 0,36 mm and 100% : 0,00 mm.

scholarly journals Uji daya hambat ekstrak biji kopi robusta (Coffea robusta) terhadap pertumbuhan Enterococcus faecalis secara in vitro

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Vilani A. M. Tilaar ◽  
Marie M. Kaseke ◽  
Juliatri .
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Seed Extract ◽  
Diffusion Method ◽  
Control Group ◽  
Robusta Coffee ◽  
Post Test ◽  
Coffea Robusta ◽  
Coffee Seed

Abstract: Enterococcus faecalis is commonly found in the root canal especially in failing treated root canal. These bacteria is resistant against many antibacterials. Recently, natural antibacterials have been developed as alternative antibacterials particularly for oral infection such as Robusta coffee seed extract (Coffea robusta) which contains antibacterial compounds (caffeine, volatile acid, and phenol). This study aimed to obtain the inhibition ability of Robusta coffee seed extract against growth of Enterococcus faecalis in vitro. This was a true experimental study with a post test only control group design. This study used agar diffusion method with well modified technique with 5 times of repetitions. The positive control was chlorhexidine 2% and the negative control was aquades. The results showed that Robusta coffee seed extract could inhibit the growth of Enterococcus faecalis with an average diameter of inhibition zone of 13.8 mm.Keywords: enterococcus faecalis, coffea robustaAbstrak: Enterococcus faecalis sering ditemukan di dalam saluran akar terutama yang gagal terhadap perawatan. Bakteri ini telah resisten terhadap banyak bahan antibakteri. Saat ini telah banyak dikembangkan bahan antibakteri alami sebagai alternatif pengobatan antibakteri khususnya untuk infeksi rongga mulut, yaitu antara lain kopi robusta (Coffea robusta) yang mengandung senyawa antibakteri (kafein, asam volatil, dan fenol). Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji kopi robusta terhadap pertumbuhan bakteri Enterococcus faecalis secara in vitro. Jenis penelitian ialah eksperimental murni (true experimental design) dengan post test only control grup design. Penelitian ini menggunakan metode difusi agar dengan teknik sumuran. Pengulangan dilakukan sebanyak lima kali. dengan kontrol positif chlorhexidine 2% dan kontrol negatif aquades. Hasil penelitian menunjukkan bahwa ekstrak biji kopi robusta (Coffea robusta) memiliki daya hambat terhadap pertumbuhan bakteri Enterococcus faecalis dengan rerata diameter zona hambat 13,8 mm.Kata kunci: Enterococcus faecalis, Coffea robusta

Antimicrobial Efficacy of a Novel Root Canal Irrigant Made from Coconut and Bromelain Extract against Biofilm Forming Pathogens– An In vitro Evaluation

2021 ◽  
pp. 5222-5226
Author(s):  
Jerry Jose ◽  
Ajitha Palanivelu
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Measured Quantity ◽  
Zone Of Inhibition ◽  
Root Canal Irrigant

Objective: Sodium hypochlorite is shown to be a gold standard intracanal irrigant which has shown to have adequate antimicrobial properties and tissue dissolving properties. The toxicity of these irrigants are seen to be high towards various cells. Natural irrigants provide an alternative to show adequate antimicrobial activity and lesser cytotoxicity potential. The current study proposes a novel intracanal irrigant made from coconut and bromelain extract. Material and Methods: 10grams of sodium hydroxide was dissolved in deionized water followed by which 60ml of coconut oil was added to the warm water bath (40C). The final product was left to dry and crushed manually to powder. This powder was then taken in measured quantity to make a final product containing 20% of coconut and 4% of bromelain. The obtained irrigant was then evaluated against Enterococcus faecalis using Agar well diffusion method. Results: For the natural irrigant, the maximum zone of inhibition was seen highest at 22mm against Enterococcus faecalis and 25mm against Candida Albicans. For 3% sodium hypochlorite when compared against Enterococcus faecalis and Candida Albicans showed a maximum zone of inhibition at 33mm against Enterococcus faecalis and 35mm against Candida Albicans. Conclusion: The study shows that the novel irrigant derived from coconut and bromelain extract has a good antimicrobial efficacy against Enterococcus faecalis and Candida albicans. It can be concluded that it has a potential application as a root canal irrigant for root canal disinfection.

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