scholarly journals Uji daya hambat ekstrak biji kopi robusta (Coffea robusta) terhadap pertumbuhan Enterococcus faecalis secara in vitro

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Vilani A. M. Tilaar ◽  
Marie M. Kaseke ◽  
Juliatri .
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Seed Extract ◽  
Diffusion Method ◽  
Control Group ◽  
Robusta Coffee ◽  
Post Test ◽  
Coffea Robusta ◽  
Coffee Seed

Abstract: Enterococcus faecalis is commonly found in the root canal especially in failing treated root canal. These bacteria is resistant against many antibacterials. Recently, natural antibacterials have been developed as alternative antibacterials particularly for oral infection such as Robusta coffee seed extract (Coffea robusta) which contains antibacterial compounds (caffeine, volatile acid, and phenol). This study aimed to obtain the inhibition ability of Robusta coffee seed extract against growth of Enterococcus faecalis in vitro. This was a true experimental study with a post test only control group design. This study used agar diffusion method with well modified technique with 5 times of repetitions. The positive control was chlorhexidine 2% and the negative control was aquades. The results showed that Robusta coffee seed extract could inhibit the growth of Enterococcus faecalis with an average diameter of inhibition zone of 13.8 mm.Keywords: enterococcus faecalis, coffea robustaAbstrak: Enterococcus faecalis sering ditemukan di dalam saluran akar terutama yang gagal terhadap perawatan. Bakteri ini telah resisten terhadap banyak bahan antibakteri. Saat ini telah banyak dikembangkan bahan antibakteri alami sebagai alternatif pengobatan antibakteri khususnya untuk infeksi rongga mulut, yaitu antara lain kopi robusta (Coffea robusta) yang mengandung senyawa antibakteri (kafein, asam volatil, dan fenol). Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji kopi robusta terhadap pertumbuhan bakteri Enterococcus faecalis secara in vitro. Jenis penelitian ialah eksperimental murni (true experimental design) dengan post test only control grup design. Penelitian ini menggunakan metode difusi agar dengan teknik sumuran. Pengulangan dilakukan sebanyak lima kali. dengan kontrol positif chlorhexidine 2% dan kontrol negatif aquades. Hasil penelitian menunjukkan bahwa ekstrak biji kopi robusta (Coffea robusta) memiliki daya hambat terhadap pertumbuhan bakteri Enterococcus faecalis dengan rerata diameter zona hambat 13,8 mm.Kata kunci: Enterococcus faecalis, Coffea robusta

scholarly journals UJI EFEKTIFITAS ANTIBAKTERI MINYAK ATSIRI SEREH DAPUR SEBAGAI BAHAN MEDIKAMEN SALURAN AKAR TERHADAP BAKTERI ENTEROCOCCUS FAECALIS

e-GIGI ◽  
2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Mario S. Howarto ◽  
Pemsi M. Wowor ◽  
Christy N. Mintjelungan
Keyword(s):  
Essential Oil ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Agar Plate ◽  
Diffusion Method ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Lemongrass Essential Oil

Abstract: Endodontics is the a type of treatment that aims to keep the teeth in function. Disinfection of the root canal is very important in endodontic treatment. Root canal disinfection can be done by giving the material a root canal medication. One of the bacteria that causes the failure of root canal treatment is the bacterium Enterococcus faecalis. The lemongrass essential oil contains geranial, neral, and mirsen which have antimicrobial activity against Gram-positive and Gram-negative. This study aimed to determine whether the lemongrass essential oil was effective against bacteria Enterococcus faecalis. This was an experimental study using post test only control group design with agar plate diffusion method. Samples consisted of a group of lemongrass essential oil with several concentrations: 25%, 50%, 75%, 100%, each consisted of 4 samples. The 16 control groups consisted of positive and negative groups. Diameter of inhibition was determined by the ability to inhibit Enterococcus faecalis cultured on MHA agar. The results showed that the average inhibitory diameter of 25% lemongrass essential oil was 2.60 mm; of 50% was 4.73 mm; of 75% was 4.50 mm; and of 100% was 5.34 mm. Conclusion: Lemongrass essential oil showed an antibacterial effect to inhibit the growth of bacteria Enterococcus faecalis.Keywords: enterococcus faecalis, lemongrass oil, antibacerialAbstrak: Endodontik merupakan salah satu jenis perawatan yang bertujuan mempertahankan gigi agar tetap dapat berfungsi. Disinfeksi saluran akar sangat penting dalam perawatan endodontik. Disinfeksi saluran akar dapat dilakukan dengan memberi bahan medikamen saluran akar. Salah satu bakteri yang menyebabkan kegagalan perawatan saluran akar ialah bakteri Enterococcus faecalis. Minyak atsiri sereh dapur mengandung geranial, neral dan mirsen yang memiliki aktifitas antimikrobapada gram positif dan gram negatif. Penelitian ini bertujuan untuk mengetahui apakah minyak atsiri sereh dapur memiliki efek terhadap bakteri Enterococcus faecalis. Penelitian ini menggunakan metode eksperimental dengan desain post test only control group design dan metode difusi lempeng agar. Sampel penelitian terdiri dari kelompok minyak atsiri sereh dapur dengan konsentrasi 25%, 50%, 75%, dan 100% yang masing-masing terdiri dari 4 sampel; 16 kelompok kontrol terdiri atas kelompok positif dan negatif. Diameter hambat ditentukan berdasarkan kemampuan menghambat Enterococcus faecalis yang dibiakkan pada media agar MHA. Hasil penelitian memperlihatkan rata-rata diameter hambat minyak atsiri sereh dapur dengan konsentrasi 25% sebesar 2,60 mm, 50% sebesar 4,73 mm, 75% sebesar 4,50 mm, dan 100% sebesar 5,34 mm. Simpulan: Minyak atsiri sereh dapur memiliki efek antibakteri untuk menghambat pertumbuhan bakteri Enterococcus faecalis.Kata kunci: enterococcus faecalis, sereh dapur, antibakteri

scholarly journals Uji Efektivitas Ekstrak Buah Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Bakteri Enterococcus faecalis Secara In Vitro

2015 ◽  
Vol 3 (2) ◽  
pp. 111-118
Author(s):  
Annisa Fajriatul Arafah ◽  
Vivi Triana ◽  
Murniwati Murniwati
Keyword(s):  
Citric Acid ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Bacterial Cell ◽  
Early Stage ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Citrus Aurantifolia

Enterococcus faecalis is a normal microorganism in root canal teeth, anaerobic facultative, and gram positive cocci. This bacteria is opportunistic that cause secunder infection in root canal. Lime (Citrus aurantifolia) is one of the plants which have antimicrobial activity because lime contains flavonoid and citric acid. Flavonoids can denature proteins in bacterial cell and damaging the cell membrane. Citric acid can damaging the bacterial cell wall and inhibiting bacterial enzymes activity. The purpose of this study was to examie the effectiveness lime extract in various concentration in inhibiting Enterococcus faecalis in in-vitro. The research method was experimental laboratories with posttest only control group design. Samples were used in this research are Enterococcus faecalis bacteria in M. Djamil’s Hospital Microbiology Laboratory, Padang. This research was conducted at organic chemistry of natural materials laboratory FMIPA UNAND and Kopertis Wilayah X Padang laboratory. Early stage was done by making extracts of lime with concentrations are 25%, 50%, 75%, and 100%. Then, the extracts was tested into the bacteria with the diffusion method used papper disk. The inhibition zone formed between lime extracts in various concentrations to the growth of Enterococcus faecalis were calculated using calipers. Data was analyzed by using Kruskal Wallis test and Mann-Whithney test with confidence interval of 95%. The result presented that 25%, 50%, 75%, and 100% lime extracts have inhibition activity in inhibiting the growth of Enterococcus faecalis, and the higher the concentration of the extracts, the greater the inhibition zone formed.

scholarly journals Uji Daya Hambat Ekstrak Kulit Langsat (Lansium parasiticum) Terhadap Pertumbuhan Bakteri Enterococcus faecalis Secara In Vitro

2020 ◽  
Vol 8 (2) ◽  
pp. 70-74
Author(s):  
Andhini Ardi ◽  
Dedi Sumantri ◽  
Surma Adnan
Keyword(s):  
Enterococcus Faecalis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Fruit Peel ◽  
Control Group Design ◽  
Inhibition Effect ◽  
Facultative Anaerobic Bacteria ◽  
Peel Extract

Enterococcus faecalis is a bacteria that commonly found in secondary root canal infections. Enterococcus faecalis is a gram-positive, facultative anaerobic bacteria. Langsat (Lansium parasiticum) is a natural ingredient that contains secondary metabolites and has antibacterial ativity. The aim of this study was to examine the inhibition effect of langsat fruit peel extract (Lansium parasiticum) to the growth of Enterococcus faecalis. This was a laboratory experimental study with post-only control group design. Langsat fruit peel extract with 25%, 50%, 75%, and 100% concentration was using disc diffusion method on Mueller-Hinton Agar (MHA) and the inhibition zone was measured with a sliding caliper. The data were analyzed with Kruskal-Wallis test and Mann-Whithney test. The result of this study was langsat fruit peel extract with 25%, 50%, 75% concentration showed a inhibition zone, while at 100% showed no inhibition zone. The Kruskal-Wallis test showed that there are significant differences (p <0.05) between all groups. The conclusion was langsat fruit peel extract (Lansium parasiticum) have weak inhibition effect of the growth of Enterococcus faecalis bacteria. The inhibition zone of concentrations 25% : 2,20 mm;  50%: 0,94 mm; 75%: 0,36 mm and 100% : 0,00 mm.

scholarly journals DAYA ANTIBAKTERI EKSTRAK DAUN KEDONDONG BANGKOK (Spondias dulcis Forst.) TERHADAP BAKTERI Enterococcus faecalis Antibacterial Potency of Kedondong Bangkok Leaves Extract (Spondias dulcis Forst.) against Enterococcus faecalis Bacteria

2016 ◽  
Vol 6 (2) ◽  
pp. 110
Author(s):  
Singgih Harseno ◽  
Latief Mooduto ◽  
Eric Priyo Prasetyo
Keyword(s):  
Minimal Inhibitory Concentration ◽  
Enterococcus Faecalis ◽  
Bacterial Growth ◽  
Root Canal ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Minimal Bactericidal Concentration ◽  
Control Group Design ◽  
Endodontic Infection ◽  
Post Test

Background. The prevalence of endodontic infection after root canal treatment caused by the Enterococcus faecalis bacteria ranged between 24-77%. It is caused by resilience and virulence from Enterococcus faecalis. An alternative  solution have to be done toward irrigation of root canal wall which is effective to kill bacteria. Kedondong Bangkok (Spondias dulcis Forst.) is one of the plants or natural substance potentially as an antibacteria. The antibacterial potencies of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria could be identified by determining Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). Purpose. This study is aimed to prove antibacterial potencies by identifying Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria. Method. This study is an experimental laboratories through research design of The Post Test Only Control Group Design. Value of MIC and MBC were known by counting the growth of Enterococcus faecalis bacteria by treating the leaves extract of Kedondong Bangkok  with concentration respectively 25%, 22,5%, 20%,17,5%, 15%, and 12,5% on nutrient agar media in CFU/ml. Result. In the concentration 12,5% there are 8.8% bacterial growth and in the concentration 15% there are no bacterial growth. Conclusion. Kedondong Bangkok leaf extract (Spondias dulcis Forst.) has an antibacterial potency against Enterococcus faecalis bacteria. The MIC shows in concentration of 12,5% and the MBC shows in concentration of 15%.

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy, Modified Triple Antibiotic Paste and Calcium Hydroxide on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2019 ◽  
Vol 10 (5) ◽  
pp. S23-S29 ◽  
Author(s):  
Mohammad Asnaashari ◽  
Mohammad Jafar Eghbal ◽  
Amirali Sahba Yaghmayi ◽  
Mehdi Shokri ◽  
Saranaz Azari-Marhabi
Keyword(s):  
Photodynamic Therapy ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Control Group ◽  
Root Canals ◽  
Anterior Teeth ◽  
Triple Antibiotic Paste ◽  
Working Length

Introduction: A pivotal issue to achieve success in the treatment of the root canal is root canal disinfection. One of the most important bacteria that infect the root canal is Enterococcus faecalis. This study seeks to examine the effectiveness of 3 methods for disinfecting the root canal: photodynamic therapy, modified triple antibiotic paste (MTAP), and calcium hydroxide. Methods: Sixty-two single-rooted extracted anterior teeth were collected. After cleaning and disinfecting the teeth, their crowns were cut at the CEJ point. The root canals were shaped to the working length up to file F3 ProTaper (F1, F2, F3). EDTA 17% and sodium hypochlorite 2.5% were used for 5 minutes to wash and remove the smear layer, and then the apical foramen was sealed using composite. After that, the teeth were sterilized in an autoclave at 121°C for 15 minutes. Then 10 samples were taken randomly as the negative control. The remaining samples were immersed and cultivated in a suspension containing E. faecalis for 21 days. Then the samples were divided into 5 groups: 2 positive control groups, 1 group treated with the antibiotic paste with the concentration of 1 mg/mL, 1 group treated with calcium hydroxide, and 1 group treated with photodynamic therapy. Then, to collect the biofilm, the ProTaper file F4 was used. After that, the microbial suspension was provided and counting the colonies was carried out to compare the groups. Results: The findings indicated that the amount of CFU/mg of MTAP samples, including clindamycin, metronidazole, ciprofloxacin in the concentration of 1 mg/mL and photodynamic therapy and calcium hydroxide was lower than that in the control group. Antibiotic paste cleansed the root canal up to 99.9%. Photodynamic therapy reduced the amount of CFU/mg to 98.8%, and calcium hydroxide reduced the amount of CFU/mg to 94.13%. Conclusion: Using photodynamic therapy causes a reduction in the biofilm and inhibits the growth of the E. faecalis bacterium. In addition, in this study, MTAP with a concentration of 1 mg/mL was used, which expunged the bacteria completely. Meanwhile, calcium hydroxide had the weakest effect of all on the E. faecalis bacterium.

scholarly journals Effectiveness of light-emitting diode exposure on photodynamic therapy against Enterococcus faecalis: in vitro study

2020 ◽  
Vol 53 (2) ◽  
pp. 71
Author(s):  
Nanik Zubaidah ◽  
Agus Subiwahjudi ◽  
Dinda Dewi Artini ◽  
Karina Erda Saninggar
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Control Group ◽  
Root Canal Irrigation ◽  
Group I ◽  
Significant Difference

Background: A successful root canal treatment eliminates pathogenic bacteria from infected root canals. The most common bacteria in root canal infections is Enterococcus faecalis (E. faecalis), due to its resistance to medicament and root canal irrigation. A photodynamic therapy (PDT) is a method of root canal disinfection that uses a combination of photosensitisers and light activation to eliminate bacteria in the root canal. The duration of the PDT irradiation results in the production of singlet oxygen and reactive oxygen species (ROS) to eliminate the E. faecalis bacteria. Purpose: To analyse the differences in the duration exposure of photodynamic therapy against the E. faecalis bacteria. Methods: The E. faecalis bacteria culture was divided into seven eppendorf tubes. Group I was a control group, and group II, III, IV, V, VI and VII were treated using PDT consisting of Toluidine Blue O (TBO) photosensitiser and light source irradiation for ten, 20, 30, 40, 50 and 60 seconds, respectively. After incubation, the number of bacteria was calculated by the Quebec Colony Counter and analysed using the Kruskal–Wallis test and the Mann–Whitney test (p <0.05). Results: There was a significant difference between the number of E. faecalis bacteria colonies in each treatment group (p <0.05). Group VI and VII, which had a longer exposure to PDT, showed a smaller amount of E. faecalis bacteria. Conclusion: The longer exposure of PDT results in a smaller amount of E. faecalis bacteria. The light irradiation of 50 seconds is the most effective to eliminate E. faecalis bacteria.

scholarly journals The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus

2021 ◽  
Vol 11 (2) ◽  
pp. 67
Author(s):  
Tamara Yuanita ◽  
Dian Agustin Wahjuningrum ◽  
Mega Selvia
Keyword(s):  
Lactobacillus Acidophilus ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
The Difference ◽  
Peel Extract

Background: Lactobacillus acidophilus is a gram-positive rod-shaped bacteria which mostly causes chronic caries lesions. In the process of cavity preparation it is reported that it is not possible to remove all microorganisms and even bacteria can survive even after removal of the necrotic tissue. Efforts to stop the growth of these bacteria are by chemical cavity cleanser using a 5% NaOCl, but this material have disadvantages including irritating periradicular tissue, unpleasant odor, and toxicity. Due to its disadvantages, it is hoped that natural ingredients can be used as alternatives. Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial ability with a concentration of 6% according to the non-toxic minimum kill concentration of Lactobacillus acidophilus. Purpose: To analyze the difference in antibacterial power between the extract of cocoa bark with a concentration of 6% compared to 5% NaOCl against Lactobacillus acidophilus. Methods: This study was a laboratory experimental in vitro with a post test-only control group design. Using diffusion method against Lactobacillus acidophilus grown in tubes containing BHIB, then cultured in a petridish which contains a nutrient agar and is divided into 2 parts extract cocoa peel extract 6% and NaOCl 5%, then each petridish given a paper disc that has been poured 10μl by each material, then incubated inthe incubator for 2x24 hours at 37oC and observe the diameter of the inhibition zone formed using a caliper. Results: The average diameter of the inhibition zone formed in the 6% cocoa peel extract was 11.8375 mm and NaOCl 5% was 26.0344 mm against Lactobacillus acidophilus. Conclusion: There is a difference in antibacterial power between 6% cocoa peel extract and 5% NaOCl against Lactobacillus acidophilus.

scholarly journals Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study

2013 ◽  
Vol 3 (3) ◽  
pp. 117-120 ◽  
Author(s):  
Hina Sheth ◽  
Uday Kamath ◽  
Sai Ramesh ◽  
Keshav Singla
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Normal Saline ◽  
Diffusion Method ◽  
Saline Control ◽  
Control Group ◽  
Antibacterial Efficacy ◽  
Tea Tree Oil ◽  
Tea Tree

ABSTRACT Background and objectives Among the bacterial species which most often cause endodontic failure, Enterococcus faecalis is the most important. This study compared the antibacterial efficacy of tea tree oil with 3% sodium hypochlorite and 2% chlorhexidine as a root canal irrigant, against E. faecalis Materials and Methods Normal saline, tea tree oil, 3% sodium hypochlorite, 2% chlorhexidine and fresh stains of E. faecalis were used. Four round wells, 4 mm deep and 8 mm diameter, were punched using sterile cork borer in blood agar plates and divided into four groups: group A: normal saline (control), group B: 3% sodium hypochlorite, group C: 2% chlorhexidine and group D: tea tree oil. Agar well diffusion method was performed to compare the antibacterial efficacy of tea tree oil, 3% sodium hypochlorite and 2% chlorhexidine. Results The results were tabulated and statistically analyzed using analysis of variance (ANOVA). Tea tree oil showed comparable inhibition of bacterial growth with sodium hypochlorite and chlorhexidine. No zone of inhibition was shown by normal saline (control). Interpretation and conclusion Tea tree oil showed statistically significant antimicrobial activity against Enterococcus faecalis, which is very much similar to sodium hypochlorite and chlorhexidine. Abbreviations CHX: Chlorhexidine; E. faecalis: Enterococcus faecalis; NaOCl: Sodium hypochlorite. How to cite this article Kamath U, Sheth H, Ramesh S, Singla K. Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study. J Contemp Dent 2013; 3(3):117-120.

Comparative Analysis of Protaper and Waveone Systems to Reduce Enterococcus Faecalis from Root Canal System in Primary Molars – An in Vitro Study

2016 ◽  
Vol 40 (2) ◽  
pp. 124-128
Author(s):  
Sérgio Luiz Pinheiro ◽  
Carolina Pessoa ◽  
Josianne Neres da Silva ◽  
Rafael Orro Gonçalves ◽  
Danilo Antonio Duarte ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Wilcoxon Test ◽  
Primary Molars ◽  
Control Group ◽  
Canal System ◽  
Root Canal System ◽  
Group B

Objective: To assess, in vitro, the ability of the ProTaper™ and WaveOne™ systems to reduce Enterococcus faecalis contamination in primary molars. Study design: Sixty roots of primary molars were contaminated with E. faecalis. Roots were randomly allocated to one of four groups (n=20): ProTaper™, WaveOne™, control A, or control B. The files used were S1 and S2/F1 and F2 (ProTaper™ system) and 25.08 (WaveOne™ system). In control group A, the root canal was left uninstrumented, whereas in control group B, the root canal was irrigated with NaCl 0.9%. E. faecalis was sampled from the root canal system before and after instrumentation and the Wilcoxon test and Mann–Whitney U were used. Results: There were no differences in E. faecalis counts between pre-instrumentation counts in the ProTaper™ and WaveOne™ (p&gt;0.05). The ProTaper™ system led to an 89.36% reduction in E. faecalis burden, versus 78.10% with the WaveOne™ system (p&gt;0.05). Instrumentation time was shorter with WaveOne™ (p&lt;0.0001). Conclusions: The ProTaper™ and WaveOne™ systems were equally effective in reducing Enterococcus faecalis in primary molars. The WaveOne™ system was associated with shorter instrumentation time.

Antimicrobial Efficacy of a Novel Root Canal Irrigant Made from Coconut and Bromelain Extract against Biofilm Forming Pathogens– An In vitro Evaluation

2021 ◽  
pp. 5222-5226
Author(s):  
Jerry Jose ◽  
Ajitha Palanivelu
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Measured Quantity ◽  
Zone Of Inhibition ◽  
Root Canal Irrigant

Objective: Sodium hypochlorite is shown to be a gold standard intracanal irrigant which has shown to have adequate antimicrobial properties and tissue dissolving properties. The toxicity of these irrigants are seen to be high towards various cells. Natural irrigants provide an alternative to show adequate antimicrobial activity and lesser cytotoxicity potential. The current study proposes a novel intracanal irrigant made from coconut and bromelain extract. Material and Methods: 10grams of sodium hydroxide was dissolved in deionized water followed by which 60ml of coconut oil was added to the warm water bath (40C). The final product was left to dry and crushed manually to powder. This powder was then taken in measured quantity to make a final product containing 20% of coconut and 4% of bromelain. The obtained irrigant was then evaluated against Enterococcus faecalis using Agar well diffusion method. Results: For the natural irrigant, the maximum zone of inhibition was seen highest at 22mm against Enterococcus faecalis and 25mm against Candida Albicans. For 3% sodium hypochlorite when compared against Enterococcus faecalis and Candida Albicans showed a maximum zone of inhibition at 33mm against Enterococcus faecalis and 35mm against Candida Albicans. Conclusion: The study shows that the novel irrigant derived from coconut and bromelain extract has a good antimicrobial efficacy against Enterococcus faecalis and Candida albicans. It can be concluded that it has a potential application as a root canal irrigant for root canal disinfection.

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