Combined Effect of Low Magnesium and High Sucrose Diet on Magnesium Status and Nitric Oxide Production in Rats

A new and convenient animal model for studying peripheral vascular and coronary artery disease in diabetes was established in this study. Male New Zealand White rabbits weighing approximately 2 kg were divided into 2 groups: a normal control group fed standard laboratory chow and a diabetogenic diet–fed group received a high-fat/high-sucrose diet. The high-fat/high-sucrose diet (contained 10% lard and 37% sucrose) feeding was maintained for 6 months. Plasma total cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride, superoxide dismutase, nitric oxide, nitric oxide synthase, insulin, and glucose were quantitated at monthly or bimonthly intervals. The aortic fatty streak lesions were quantified following lipid staining with Sudan IV. The aortic samples were observed by electron microscopy. High plasma triglyceride and glucose concentrations were induced. At the end of 6 months, the aortic fatty streak lesions were present in the animals' vascular specimens. As far as we know, this is the first report that demonstrates that New Zealand White rabbits can develop obvious aortic fatty streaks by feeding a high-fat/high-sucrose diet. Our results suggest that NewZealand White rabbits fed a high-fat/high-sucrose diet would provide a convenient model for studying peripheral vascular and coronary artery disease in diabetes.

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Nox2 mediates high fat high sucrose diet-induced nitric oxide dysfunction and inflammation in aortic smooth muscle cells

Journal of Molecular and Cellular Cardiology ◽

10.1016/j.yjmcc.2014.02.019 ◽

2014 ◽

Vol 72 ◽

pp. 56-63 ◽

Cited By ~ 22

Author(s):

Zhexue Qin ◽

Xiuyun Hou ◽

Robert M. Weisbrod ◽

Francesca Seta ◽

Richard A. Cohen ◽

...

Keyword(s):

Nitric Oxide ◽

Smooth Muscle ◽

Smooth Muscle Cells ◽

Muscle Cells ◽

High Fat ◽

Aortic Smooth Muscle Cells ◽

Aortic Smooth Muscle ◽

Sucrose Diet ◽

High Sucrose Diet ◽

High Sucrose

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Chronic Effects of a High Sucrose Diet on Murine Gastrointestinal Nutrient Sensor Gene and Protein Expression Levels and Lipid Metabolism

International Journal of Molecular Sciences ◽

10.3390/ijms22010137 ◽

2020 ◽

Vol 22 (1) ◽

pp. 137

Author(s):

Patrick O’Brien ◽

Ge Han ◽

Priya Ganpathy ◽

Shweta Pitre ◽

Yi Zhang ◽

...

Keyword(s):

Nitric Oxide ◽

Bacterial Overgrowth ◽

De Novo Lipogenesis ◽

Nutrient Metabolism ◽

Expression Levels ◽

Obesity And Diabetes ◽

Gene And Protein Expression ◽

Sucrose Diet ◽

High Sucrose Diet ◽

High Sucrose

The gastrointestinal tract (GIT) plays a key role in regulating nutrient metabolism and appetite responses. This study aimed to identify changes in the GIT that are important in the development of diet related obesity and diabetes. GIT samples were obtained from C57BL/6J male mice chronically fed a control diet or a high sucrose diet (HSD) and analysed for changes in gene, protein and metabolite levels. In HSD mice, GIT expression levels of fat oxidation genes were reduced, and increased de novo lipogenesis was evident in ileum. Gene expression levels of the putative sugar sensor, slc5a4a and slc5a4b, and fat sensor, cd36, were downregulated in the small intestines of HSD mice. In HSD mice, there was also evidence of bacterial overgrowth and a lipopolysaccharide activated inflammatory pathway involving inducible nitric oxide synthase (iNOS). In Caco-2 cells, sucrose significantly increased the expression levels of the nos2, iNOS and nitric oxide (NO) gas levels. In conclusion, sucrose fed induced obesity/diabetes is associated with changes in GI macronutrient sensing, appetite regulation and nutrient metabolism and intestinal microflora. These may be important drivers, and thus therapeutic targets, of diet-related metabolic disease.

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