scholarly journals Methylation status of leptin receptor gene promoter in obese children

2020 ◽  
Vol 95 (2) ◽  
pp. 86-91
Author(s):  
Katarzyna Jakubek-Kipa ◽  
Natalia Potocka ◽  
Marzena Skrzypa ◽  
Łukasz Dembinski ◽  
Izabela Zawlik ◽  
...  
Keyword(s):  
Leptin Receptor ◽  
Receptor Gene ◽  
Methylation Status ◽  
Gene Promoter ◽  
Obese Children

Impact of leptin and leptin-receptor gene polymorphisms on serum lipids in Japanese obese children

2010 ◽  
Vol 99 (8) ◽  
pp. 1213-1217 ◽  
Author(s):  
T Okada ◽  
T Ohzeki ◽  
Y Nakagawa ◽  
S Sugihara ◽  
O Arisaka ◽  
...  
Keyword(s):  
Gene Polymorphisms ◽  
Serum Lipids ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Obese Children ◽  
Receptor Gene Polymorphisms

scholarly journals Leptin Receptor Gene Variant rs11804091 Is Associated with BMI and Insulin Resistance in Spanish Female Obese Children: A Case-Control Study

2017 ◽  
Vol 18 (8) ◽  
pp. 1690 ◽  
Author(s):  
Josune Olza ◽  
Azahara Rupérez ◽  
Mercedes Gil-Campos ◽  
Rosaura Leis ◽  
Ramón Cañete ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Leptin Receptor ◽  
Case Control Study ◽  
Receptor Gene ◽  
Case Control ◽  
Gene Variant ◽  
Obese Children ◽  
Control Study

scholarly journals Obesity in Children with Leptin Receptor Gene Polymorphisms

2021 ◽  
Vol 64 (3) ◽  
pp. 158-164
Author(s):  
Aleksandr Abaturov ◽  
Anna Nikulina
Keyword(s):  
Discriminant Analysis ◽  
Sequential Analysis ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Bioinformatic Analysis ◽  
Control Group ◽  
Nucleotide Polymorphisms ◽  
Multiple Discriminant Analysis ◽  
Obese Children ◽  
Nominal Data

Introduction: The study of single nucleotide polymorphisms (SNPs) of the leptin receptor gene (LEPR) based on next generation genomic sequencing (NGS) data is becoming an increasingly important aspect of diagnosis, treatment and prevention of both metabolically healthy (MHO) and metabolically unhealthy obesity (MUO) phenotypes. Material and methods: 35 obese children 6-18 years old were examined by the NGS method with bioinformatic analysis. The main group (n = 18) was formed by children with MUO, according to the recommendations of the expert group of the National Heart, Lung, and Blood Institute. The control group (n = 17) was represented by children with MHO. Statistical methods were used: analysis of variance, Wald’s sequential analysis, Spearman’s correlation analysis, analysis of nominal data and multiple discriminant analysis. Results: 10 types of non-synonymous SNPs (rs3790435, rs1137100, rs2186248, rs70940803, rs79639154, rs1359482195, rs1137101, rs1805094, rs13306520, rs13306522) of the LEPR gene in obese children have been identified. Multiple discriminant analysis demonstrated that the following LEPR SNPs are of greatest importance in the development of MUO: rs3790435, rs13306522, rs13306520. Analysis of nominal data revealed significant differences in the groups for Copy number variation (CNV) rs3790435 of the LEPR gene. Wald’s analysis allowed us to identify 6 important predictors of MUO (І ≥ 0.5): 2 CNV rs3790435 (Relative Risk, RR = 2, Prognostic coefficient, PC = +2.76); male gender of the child (RR = 1.3, PC = +1.35); rs3790435 (RR = 1.9, PC = +2.76); hyperleptinemia more than 40.56 ng/ml (RR = 2, PC = +3); CNV rs1359482195 ≥ 3 (RR = 1.9, PC = +5.8); SNP of the LEPR gene ≥4 (RR = 3.8, PC = +5.8). Conclusion: Children with the genotype rs3790435 gene LEPR had signs of MUO more often.

Isolation of a novel leptin receptor gene promoter preferentially functioning in neuronal cells

2009 ◽  
Vol 389 (4) ◽  
pp. 673-677 ◽  
Author(s):  
Tetsurou Satoh ◽  
Satoshi Yoshino ◽  
Akiko Katano ◽  
Takahiro Ishizuka ◽  
Takuya Tomaru ◽  
...  
Keyword(s):  
Leptin Receptor ◽  
Receptor Gene ◽  
Neuronal Cells ◽  
Gene Promoter

scholarly journals Association of leptin receptor gene polymorphisms and meta-inflammation markers with metabolically unhealthy obesity in children

2021 ◽  
Vol 23 (5) ◽  
pp. 696-702
Author(s):  
А. Е. Abaturov ◽  
A. O. Nikulina
Keyword(s):  
Leptin Receptor ◽  
Receptor Gene ◽  
Strong Association ◽  
Main Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Inflammation Markers ◽  
Obese Children ◽  
Lepr Gene ◽  
Metabolically Unhealthy Obesity

The aim: to study the contribution of single-nucleotide polymorphisms (SNP) of the leptin receptor (LEPR) gene and meta-inflammation markers to the formation of metabolically unhealthy obesity (MUO) in children. Materials and methods. A total of 109 obese children aged 6–18 years were examined. Based on the recommendations of the National Heart, Lung, and Blood Institute (NHLBI), 2 observation groups were formed. The main group (n = 56) was represented by patients with MUO. The control group (n = 53) comprised children with metabolically healthy obesity (MHO). Serum levels of interleukin-1β (IL-1β) were measured using a chemiluminescent immunoassay (CLIA) method, interleukin-6, leptin, adiponectin – by enzyme-linked immunosorbent assay (ELISA) and the serum level of C-reactive protein were quantified by latex turbidimetric method (Synevo, Ukraine). The method of next-generation sequencing (NGS) (CeXGat, Germany) was used to identify LEPR SNP. Statistical methods were used: analysis of variance, Spearman’s correlation analysis and multiple discriminant analysis. Results. In obese children aged 6 to 18 years, there was an increase in pro-inflammatory adipokines IL-6 and leptin and a decrease in anti-inflammatory adiponectin. Statistically significant changes in these indicators were more expressed in the main group: IL-6 – 7.4 ± 0.5 pg/ml (ρ = 0.65; P ≤ 0.001); adiponectin – 3.9 ± 0.8 μg/ml (ρ = -0.27; P = 0.007) among all the children examined, leptin in girls – 47.8 ± 4.4 ng/ml (ρ = -0.28; P = 0.003) compared with the results of patients in the control group: IL-6 – 4.3 ± 0.3 pg/ml, adiponectin – 7.7 ± 2.4 μg/ml, leptin in girls – 32.5 ± 4.3 ng/ml, P ≤ 0.05. The most important in the development of MUO were the following SNP of the LEPR gene: rs3790435 (CiMUO = 0.939), rs2186248 (CiMUO = 0.862), P < 0.05. A strong correlation was found between MUO and serum IL-6 level (ρ = 0.7), LEPR SNP rs3790435 (ρ = 0.7), basal hyperinsulinemia (ρ = 0.72); Р ≤ 0.001. The risk of IL-6-dependent meta-inflammation in the presence of SNP rs3790435 of the LEPR gene: OR = 17.11; 95 % CI 2.8–20.4. Conclusions. Meta-inflammation in MUO is IL-6-dependent. Among the 10 SNPs of the LEPR gene that we identified, SNP rs3790435 of the LEPR gene has a strong association with the formation of MUO. SNP rs2186248 LEPR was described by us for the first time when it was found in 94.1 % of obese children, but it was characterized by the presence of a weak association with MUO.

scholarly journals Lack of mutations in the leptin receptor gene in severely obese children

2012 ◽  
Vol 56 (3) ◽  
pp. 178-183 ◽  
Author(s):  
Natasha Favoretto Dias ◽  
Ariana Ester Fernandes ◽  
Maria Edna de Melo ◽  
Heidi Lui Reinhardt ◽  
Cintia Cercato ◽  
...  
Keyword(s):  
Leptin Receptor ◽  
Receptor Gene ◽  
Hdl Cholesterol ◽  
Molecular Study ◽  
Serum Glucose ◽  
Clinical Feature ◽  
Coding Region ◽  
Obese Children ◽  
Exon 2 ◽  
Low Hdl

OBJECTIVE: To analyze the LEPR gene in obese children and to investigate the associations between molecular findings and anthropometric and metabolic features. SUBJECTS AND METHODS: Thirty-two patients were evaluated regarding anthropometric characteristics, blood pressure, heart rate, serum glucose, insulin, leptin levels, and lipid profile. The molecular study consisted of the amplification and automatic sequencing of the coding region of LEPR in order to investigate new mutations. RESULTS: We identified a high prevalence of metabolic disorders: impaired fasting glucose in 12.5% of the patients, elevated HOMA-IR in 85.7%, low HDL-cholesterol levels in 46.9%, high triglyceride levels in 40.6%, and hypertension in 58.6% of the patients. The molecular study identified 6 already described allelic variants: rs1137100 (exon-2), rs1137101 (exon-4), rs1805134 (exon-7), rs8179183 (exon-12), rs1805096 (exon-18), and the deletion/insertion of the pentanucleotide CTTTA at 3'untranslated region. CONCLUSIONS: The frequency of alleles observed in this cohort is similar to that described in the literature, and was not correlated with any clinical feature. The molecular findings in the analysis of the LEPR did not seem to be implicated in the etiology of obesity in these patients.

Association of Gln223 Arg polymorphism of the leptin receptor gene with indicators of energy expenditure in obese children

Nutrition ◽  
2014 ◽  
Vol 30 (7-8) ◽  
pp. 837-840 ◽  
Author(s):  
Judit Répásy ◽  
Szilvia Bokor ◽  
Éva Erhardt ◽  
Dénes Molnár
Keyword(s):  
Energy Expenditure ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Obese Children

Effect of genetic variation in the leptin gene promoter and the leptin receptor gene on obesity risk in a population-based case-control study in Spain

2006 ◽  
Vol 21 (8) ◽  
pp. 605-612 ◽  
Author(s):  
Olga Portolés ◽  
José Vicente Sorlí ◽  
Francesc Francés ◽  
Oscar Coltell ◽  
Jose I. González ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Leptin Receptor ◽  
Case Control Study ◽  
Receptor Gene ◽  
Gene Promoter ◽  
Population Based ◽  
Case Control ◽  
Obesity Risk ◽  
Control Study

Association between leptin receptor gene polymorphism and quality of both meat and back fat in large white pigs of ukrainian breeding

2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko
Keyword(s):  
Gene Polymorphism ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Intramuscular Fat ◽  
Large White ◽  
Pig Breeds ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

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