Association between leptin receptor gene polymorphism and quality of both meat and back fat in large white pigs of ukrainian breeding

2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko
Keyword(s):  
Gene Polymorphism ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Intramuscular Fat ◽  
Large White ◽  
Pig Breeds ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

GENETICS AND POPULATION PRACTICABILITY OF USING SNP (C. 232Т>А) OF LEPR GENE AS A MARKER FOR FURTHER SELECTION FOR LARGE WHITE AND MYRGOROD PIG BREEDS

2016 ◽  
Vol 52 ◽  
pp. 176-180 ◽  
Author(s):  
N. K. Sarantseva ◽  
V. M. Balatsky ◽  
V. Y. Nor ◽  
Ye. K. Oliinychenko
Keyword(s):  
Selection Pressure ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Large White ◽  
Weinberg Equilibrium ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed ◽  
Hardy Weinberg Equilibrium ◽  
Selection For

Leptin is an important regulator of energy metabolism and reproduction and is mainly synthesized in the adipocytes and then secreted into bloodstream. Leptin receptor is one of regulating components of organism energetic homeostasis. Receptor influences on leptin effects by regulating feed intake, body weight and fat deposition. Leptin receptor gene (LEPR) is located in the sixth chromosome in the region that correlates with content of intramuscular fat, thickness of back fat, growth rate and pig carcass parameters. Due to these correlations, LEPR is known to be gene candidate that controls quantitative traits. Leptin receptor gene consists of 20 exons; not less than 25 single nucleotide polymorphisms (SNPs) were found in gene structure in different gene sites (exons, introns, 5’ and 3’ regions). SNPs of LEPR gene can be chosen as useful markers for predicting breeding value in pigs. For the experiment SNP c.232T>A was chosen; it is located in the second exon of LEPR gene. The aim of work was to study spreading of SNP c.232Т>А in LEPR gene of breeds under Ukrainian selection; to estimate if marker selection for proving meat quality is possible using chosen SNP as a marker. Materials and methods. For genetic population analysis, DNA samples of Large White breed (bred in Stepne farm, Poltava region, Ukraine) and Mirgorod breed (bred in Dekabristy farm, Poltava region, Ukraine) were used; 50 samples of each breed were taken for the research. Samples were genotyped using PCR-RFLP method. Deviations from genetic equilibrium found using the Hardy-Weinberg coefficient were signified with chi-square criterium, the frequency of alleles, estimation of gene frequencies, determination of heterozygosity were counted using GenAlex 6.0. Results. Genetic researches showed polymorphism c. 232Т>А in LEPR gene to be spread in population of Large White breed and Mirgorod breed under Ukrainian selection. Polymorphism with AA genotype was shown to be spread the most. In studied Large White population highly probable deviation of the actual distribution of genotypes of the expected value for the Hardy-Weinberg equilibrium (χ2 = 15.759, p ≤ 0.001) was found. The deviation was caused by increasing homozygotes (АА = 0.680). Small amount of heterozygotes (АТ = 0.160) and alternative homozygotes (ТТ = 0.160) was found. Positive designation of Rayt index (0.561) and the advantage of expected heterozygosis (0.365) on the actual (0.160) also show existence of selection pressure of LEPR in this herd. In Myrgorod pig population big amount of animals turned out to be homozygotes АА (0,720), small amount of heterozygotes was found (АТ=0.280), alternative homozygotes TT were not found. Deviation from spreading of genotypes of the expected value for the Hardy-Weinberg equilibrium was not significant and did not have a significant nature (χ2 = 1.325); SNP variety (c. 232Т>А) in LEPR gene is not spread, so this SNP in Mirgorod breed wasn’t under selection pressure. The fact of low selection pressure of (c. 232Т>А) in LEPR gene in Mіrgord breed can also be proved of negative designation of Rayt index (-0,163) and domination of heterozygotes (0.280). Allele A is found to be dominative above allele T in both studied populations. Conclusions. After DNA analysis of two breeds under Ukrainian selection (Mirgorod and Large White breeds) polymorphism c. 232Т>А in LEPR gene SNP was found to be spread; chosen SNP can be used for further researches in association analysis for finding correlation between SNP and meat traits.

Indexes of reproductive ability and their repeatability in sows of different genotypes, taking into account the polymorphism g.1426g> a of the Mc4r gene

2020 ◽  
pp. 49-62 ◽  
Author(s):  
Viktor Khalak ◽  
Vasyl Voloshchuk ◽  
Konstiantyn Pochernyaev ◽  
Serhii Smyslov ◽  
Maria Ilchenko
Keyword(s):  
Receptor Gene ◽  
Live Weight ◽  
Multiple Births ◽  
Large White ◽  
Mc4r Gene ◽  
Litter Weight ◽  
Reproductive Ability ◽  
Large White Breed ◽  
White Breed ◽  
Consolidation Coefficient

The results of studies of the reproductive ability of sows of different genotypes are given taking into account the polymorphism g.1426G> A of the MC4R gene, the factor of repeatability of characters is determined, and the economic efficiency of the research results is calculated. The experimental part of the research was carried out in the conditions of the «Druzhba-Kaznacheevka» LTD in Dnipropetrovsk region. The objects of research were sows of the Large White breed. It was determined that sows of the main herd, according to signs of reproductive ability, belong to class I and class elite. The experiment showed that sows of genotype AG exceeded their peers of others (GG and AA) in terms of “born piglets total, heads” by 1.6 and 0.7 heads, “Live piglets born (multiple births), heads - by 1 6 and 0.9 heads, “the mass of the litter at the time of birth, kg” - by 2.7 - 1.3 kg., “the mass of the litter at the time of weaning at the age of 28-35 days, kg” - by 5, 1 - 3.9 kg. The difference between animals of the indicated genotypes is: for large-foetus - 0.06-0.01 kg, the index "evenness of the sow’s litter in live weight of piglets at birth, points" - 1.05-1.84 points, index of N. D. Berezovsky - 3.75 -2.36 points. The index of the safety of piglets before weaning ranged from 84.4 to 93.0 %. It was proved that the minimum value of the index “evenness of the sow’s littar in live weight of piglets at the time of birth, points (7.76 ± 1,082 points) are characterized by sows of genotype AA, in which the rate of multiple pregnancy ranged from 8 to 14 pigs, and the value of the sign“ large-foetus, kg "equaled 1.37 kg. The repeatability coefficient for sows of the main herd and sows of the Large White breed of different genotypes for the melanocortin 4 (MC4R) receptor gene ranges from -0.916 ± 0.1418 (І-IV farrow, genotype - GG, sign - “piglet safety before weaning, %) to 0.978 ± 0.0738 (I-V farrow, genotype - GG, sign - “large foetus”, kg ”). The maximum value of the phenotypic consolidation coefficient was found in sows of genotype AA according to the following quantitative characteristics: “live piglets (multiple births), heads.”, “litter weight at the time of birth, kg”, “litter weight at weaning age of 28-35 days, kg "," the safety of piglets before weaning ,% ". The sows of the genotype GG belong to the unconsolidated group according to the signs of reproductive qualities. The use of sows of the genotype for the melanocortin 4 (MC4R) AG receptor gene provides additional products at the level of 5.15 %, and its cost is 165.41 UAH /head. Keywords: sow, breed, reproductive ability, MC4R gene, genotypic consolidation coefficient, variability, correlation, repeatability.

scholarly journals Hybridization as a method of improving quality of pork

2020 ◽  
pp. 31-35
Author(s):  
B. P. Kovalenko ◽  
◽  
O. B. Shevchenko ◽  
Keyword(s):  
Chemical Composition ◽  
Physical Properties ◽  
Ph Value ◽  
Longissimus Dorsi ◽  
Large White ◽  
Group Iii ◽  
Large White Breed ◽  
White Breed ◽  
A Share

Studies were carried out on the main indicators of the chemical composition and physical properties of muskulus longissimus dorsi of pigs of different genotypes during hybridization. The indicators of the chemical composition and physical properties of muskulus longissimus dorsi were determined by conventional methods. The materials of experimental studies were processed using methods of variation statistics. According to the amount of moisture, no significant difference between the control and experimental groups was established. According to the amount of protein in meat, a tendency to a slight decrease, compared with the control group by 1.4 (group III) … 1.9 % (group II) was established. The use of boars of Poltava meat breed led to a decrease in intramuscular fat in descendants by 0.25 % compared with peers of a large white breed (P <0.95), and precocious meat - to a real increase of this indicator by 0.43 % at P> 0 , 95. A tendency towards an increase in the amount of tryptophan (by 0.04 ... 0.05) and oxyproline (by 0.004 ... 0.005) in the muscles of hybrid animals was revealed in comparison with peers of pure breed of large white breed. In terms of the biological usefulness of meat proteins, animals of group III were the best - in terms of the quality of the protein of the longest muscle of the back, their advantage over peers of group II was 0.07, and compared with purebred peers. Group I - 0.12 (p <0.95). It was found that with the same area of the meat spot both in purebred large white breeds of animals and hydrides of different genotypes, there is a tendency to increase the latter compared to the control in a humid place: with a «share» of «blood» ½ pm by 2.2 %, and with a «share» of blood ½ cm - 1.4 %. A tendency has been established to increase the pH value of the longest back muscle in animals with a «share» of blood of specialized meat breeds compared with a large white breed. In hybrid animals with a «share» of blood of Poltava meat and precocious meat breeds, there is a tendency to increase the pH value in comparison with the large white breed by 0.15 (2.8 %) and by 0.17 (3.1 %), i.e. the meat of hybrid pigs, the pH of which has a shift towards alkalinity due to less accumulation of lactic acid, has a worse preservation.

scholarly journals IMPROVMENT THE BIOLOGICAL VALUE OF PORK

2020 ◽  
Author(s):  
G.M. Shulaev ◽  
◽  
R.K. Milushev
Keyword(s):  
Vitamin E ◽  
Feed Additive ◽  
Control Group ◽  
Vitamin B ◽  
Soya Flour ◽  
Large White ◽  
Biological Value ◽  
Large White Breed ◽  
White Breed

The purpose - improve the quality of pig farming production through the use of functional feed additive at the final stage of feeding. The additive ingredients (in %): vitamin E - 0,32; vitamin D₃ - 0,30; vitamin C - 0,30; vitamin B₄ - 1,56; vitamin B₁₂ - 0,004; betaine - 10,00; %; bentonite - 77,166; Omek J – 0,15%; selen - 0,2 %; soya flour (filling substance) - 10,00. Components activity: vitamin E-50,0 of %; D₃ - 0,15,0 thousand ME in 1g; B₄ - 60,0 %; B₁₂ - 1,0%; Omek J - 2,0%; seleno-KI - 0,2 %. The functional feed additive is approved on fattened pigs of large white breed. Animals from control group received the mixed fodder constantly used in a factory, skilled - same as well as control, but the containing 1 % of the additive within 10 days before slaughter. Growth, some indicators of a metabolism, quality of meat are studied.

scholarly journals Polygenic hereditary traits of young pigs and their association with the melanocortin receptor gene – 4 (MC4R)

2020 ◽  
Vol 22 (93) ◽  
pp. 84-89
Author(s):  
V. I. Khalak ◽  
B. V. Gutyj
Keyword(s):  
Receptor Gene ◽  
Live Weight ◽  
Melanocortin Receptor ◽  
Thoracic Vertebrae ◽  
Large White ◽  
Large White Breed ◽  
Young Pigs ◽  
Melanocortin 4 Receptor Gene ◽  
White Breed ◽  
Fat Thickness

The article presents the results of studies of fattening and meat qualities of young pigs of different genealogical lines of large white breed and genotypes by the gene of melanocortin receptor – 4 (MC4R). The research was conducted in “Druzhba-Kaznacheyivka” LLC, the genetics laboratory of the Institute of Pig Breeding and APV NAAS and the animal husbandry laboratory of the State Institution Institute of Grain Crops NAAS. The work was performed according to the research program of NAAS №30 “Innovative technologies of breeding, industrial and organic production of pig products” (“Pig Breeding”). DNA-typing of the melanocortin-4 receptor gene (MC4R) was performed by Kim K., Larsen N., Short T. et al. (2000). Evaluation of young pigs for fattening and meat qualities was carried out taking into account the following quantitative characteristics: the average daily increase in live weight during the period of control fattening, g; age of live weight 100 kg, days; fat thickness at the level of 6–7 thoracic vertebrae, mm; the length of the cooled carcass cm; the length of the bacon half of the cooled half-carcass, cm. The length of the cooled carcass was measured with a measuring tape from the edge of the fusion of the pubic bones to the anterior surface of the first cervical vertebra; the length of the bacon half of the cooled half-carcass - from the anterior edge of the pubic bone to the middle of the anterior edge of the first rib (Berezovsky, Khatko, 2005). Conditions for feeding and keeping young pigs of the experimental groups were identical and complied with zootechnical standards. Economic efficiency of use of animals of various genotypes (МС4RАА, МС4RАG) and biometric processing of the received data (Lakin, 1990). calculated according to generally accepted methods. It is established that young pigs of large white breed of controlled population at the age of reaching live weight of 100 kg exceed the minimum requirements of the elite class by 6.73, fat thickness at the level of 6–7 thoracic vertebrae – 30.84, length of chilled carcass – 3.52 %. Significant difference between animals of different genotypes by melanocortin 4 receptor gene (МС4RАА, МС4RАG) was established by the average daily gain of live weight during the control period of fattening, the age of reaching live weight of 100 kg, fat thickness at the level of 6–7 thoracic vertebrae, length half of the cooled half-carcass and a complex index of fattening and meat qualities (B. Tyler's index). Young pigs of large white breed line Kotilo UA 8819347 outperformed peers line Azuro UA 8800557 on average daily live weight gain during the control period of fattening by 5.29%, the age of 100 kg live weight – 3.29 %, fat thickness at the level of 6–7 breasts vertebrae – 7.14 %. The longer length of chilled carcass (by 1.13 %) and the length of bacon half of chilled half carcass (by 2.68 %) were characterized by animals of Azuro UA 8800557. The correlation coefficient between fattening and meat qualities in young pigs of large white breed of the controlled herd varies ranging from –0.865 (tr = 22.26, P < 0.001) to +0.913 (tr = 35.43, P < 0.001). The maximum increase in additional products was obtained from young pigs of the МС4RАG genotype (+2.57 %) and the genealogical line Kotilo UA 8819347 (+4.86 %), and its cost is +694.26 – 1312.88 UAH/head. in accordance.

scholarly journals Use of specialized breeds for increase of pork meat quality

1970 ◽  
Vol 21 ◽  
pp. 238-242
Author(s):  
O. V. Boiko ◽  
O. V. Vashchenko ◽  
M. S. Nebylytsia
Keyword(s):  
Meat Quality ◽  
Pork Meat ◽  
Selection Methods ◽  
Large White ◽  
Muscle Area ◽  
Large White Breed ◽  
National University ◽  
White Breed ◽  
Conventional Methods

Aim. Matching ability to study for reproductive, fattening and meat qualities of pigs and to identify the most effective combinations of genotypes national and foreign selection. Methods. Studies conducted in conditions of industrial technology of LLB "Mayak-Agro" Cherkassy region. Groups formed by from counterpart’s methods. Assessment fattening and meat quality was carried out by conventional methods (Polivoda A., 1977). The chemical composition and physical properties of meat were determined by conventional methods in condition of biotechnology laboratories Cherkassy National University. B. Khmelnitsky. Results. The highest quantity offspring obtained by crossing hybrids with boars red white girdle (RWG) and pietrain (P) (11.6–11.8 piglets), with big fetus – when two breed crossed Ukrainian large white breed (ULW) sows of landrace (L) boars and sows of crossbred boars two bread from (P) (1.8 kg, P>0.99). By weight of one head at weaning the best results are obtained by crossing two breeds ewes from boars (RWG) and (P) – 18.1–17.8 kg (P > 0.999). Maximum saving values obtained when combined hybrids (ULW x L) with boars breed Duroc Ukrainian selection “Steppes” (DUSS) – 94.8 %. Conclusions. To improve the meat quality of pork, the best option is a combination of two breeds pig breeds (ULW x L) of boars (P) and (RWG). Keywords: hybridization, a specialized type, industrial crossing, heterosis, muscle area.

scholarly journals Продуктивність в’єтнамських вислочеревних свиней та їх помісей

2013 ◽  
pp. 73-75
Author(s):  
В. Є. Усачова
Keyword(s):  
Meat Quality ◽  
Growth And Development ◽  
Multiple Pregnancy ◽  
Large White ◽  
Slaughter Weight ◽  
Reproductive Ability ◽  
Large White Breed ◽  
White Breed

Наведено результати дослідження репродуктивних, відгодівельних і м’ясних якостей свиней в’єтнамської вислочеревної породи «в чистоті» та в поєднанні з плідниками великої білої породи. Встановлено, що за використання схрещування досягається поліпшення відтворювальної здатності свиноматок за багато-, великоплідністю, живою масою одного поросяти і гнізда при відлученні. Підсвинки в’єтнамської вислочеревної породи поступалися помісному молодняку за інтенсивністю росту і розвитку. Відгодівля помісних генотипів сприяла поліпшенню відгодівельних та м’ясних якостей. The results of the study of reproductive, fattening and meat quality of pigs in Vietnam loose belly breed «in a cleanness» and in crossing with the male hogs of large white breed are given in the article. It is set that the use of crossing leads to improvement of reproductive ability of sows on multiple pregnancy, large fetus, to living mass of one pigling. Gilts of the Vietnamese loose belly breed yielded to the crossbreed sapling on intensity of growth and development and slaughter-weight. Fattening of crossbreed genotypes was instrumental in the improvement of fattenings and meat qualities.

scholarly journals ADIPOSE TISSUE AS THE MOST IMPORTANT ELEMENT DETERMINING THE QUALITY OF PORK

PIG-BREEDING ◽  
2021 ◽  
pp. 23-26
Author(s):  
I.F. GORLOV ◽  
◽  
M.I. SLOZHENKINA ◽  
V.A. BARANIKOV ◽  
A.A. MOSOLOV ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Efficiency ◽  
Group Iv ◽  
Large White ◽  
Large White Breed ◽  
Young Pigs ◽  
Synthetic Amino Acids ◽  
White Breed ◽  
Positive Effect

The article presents the materials of research conducted on fattening growing young pigs of large white breed, in the diet of which synthetic amino acids were used. As a result of experiments, the high efficiency of their use was confirmed. The positive effect of the use of synthetic amino acids on the formation of more optimal indicators of the chemical composition of fat is proved. Experiments have shown that the most effective productive effect of feed was shown in group IV animals that received a basic diet balanced in amino acids due to the introduction of synthetic lysine and feed methionine.

scholarly journals СOMBINATIONAL ABILITY OF SPECIALIZED BREEDS AND TYPES OF PIGS IN INDUSTRIAL CROSSBREEDING

2017 ◽  
Vol 53 ◽  
pp. 84-90 ◽  
Author(s):  
O. Vashchenko
Keyword(s):  
Meat Quality ◽  
Combining Ability ◽  
Live Weight ◽  
Individual Species ◽  
Large White ◽  
Large White Breed ◽  
Wide Range ◽  
White Breed ◽  
Conventional Methods

For several decades, widespread industrial crossing different breeds of pigs. However, intensive importation foreign breeding of pig and use foreign technologies, a prerequisite for improving meat and slaughter quality of livestock. Success in solving this problem depends on identifying the best combinations of parental pairs with a comprehensive study of regularities succession and the descendants of preferred breeding traits are determined by genes and polymer characterized by a wide range of variability influenced by environmental conditions. In terms of industrial use of the most successful combinations of lines, genotypes, even a slight increase productivity, ensure, ultimately, significant economic effects. The aim of our study was to evaluate the general (GCA) and specific (SCA) combining ability fattening and meat quality of pigs and calves landed determine the best options for combinations of parental pairs when crossed. Studies were conducted in conditions of industrial technology at without belt dependent concentrations type of feeding and regulated microclimate on the basis of LLB "Mayak-Agro" Cherkassy region. Groups breeder was formed by unique origin, age, physiological condition, body weight. Assessment fattening, meat quality, physical and chemical properties of meat and fat conducted by conventional methods Polivoda AM and else. The morphological composition of carcasses studied by diking corps. The chemical composition and physical properties of meat were determined by conventional methods in condition of biotechnology laboratories Cherkassy National University. B. Khmelnitsky. In the first stage crossed sows of Ukrainian large white breed (UVB) with boars Landrace (LA) English selection, and received from them hybrids (hybrids F1) sires with specialized meat breeds: Pietrain (P) British breeding red white girdle (RWG) and Duroc Ukrainian selection “Steppes” (DUSS). It is established that the use of these industrial crossbreeding schemes leading to improved slaughter yield of 4.4 - 8.4% in the carcasses of local groups. Severity of heterosis for slaughter relies was in the range of 0.16 to 1.88%. Combining breeds LA and UVB has increased the length of the carcasses in hybrids by 2.5 cm (P> 0.95) at the lowest standard deviation - 0.14 cm. At the same time, crossing two-hybrids local pig breeds of boars’ P led rather to reduce the carcasses length compared to the original parental forms 2.6 cm (P> 0.95). Regarding mass index posterior third corps tends to increase it to 11.3 - 12.5 kg accurate advantage of using Landrace breeds (P> 0.95) and pietrain (P> 0.999). The fact that the carcasses obtained from pigs combination 1/4UVB x 1/4LA x 1/2P, were significantly shorter and had the largest area "muscle eye" is quite natural and due to the influence of breeds belonging boars. The efficacy of the combination of maternal two-breeds form hybrids with UVB × LA boars pietrain and red white girdle, confirmed the calculated values of general and specific combining ability. When using boars breed red white girdle highest positive effect of general combining ability was observed in terms of area "muscle eye" (4.94) meeting of body (0.93) slaughter yield (0.35). In addition, the use of the scheme for industrial crossing red white girdle helped reduce fat content in carcass and thick bacon (- 1.41 - 0.64). Increasing the length of the carcass at slaughter hogs promoted the use of Duroc Ukrainian selection “Steppes” (1.32) and Landrace (1.08). Boars used breeds influenced fattening and greasy meat quality of offspring with different force. Its value depended on the individual animal and breeds consolidation for the identified - from negative 8.49 to positive 6.71% age achieve live weight of 100 kg and from minus 9.34 to positive 6.34% in the average daily increments. Differential power of selective effects on offspring among individual species ranged from (- 9.34) to (+6,71)% (P> 0,999). Given the importance of meat quality indicators as signs core products finals pig should always check the structural elements of Ukrainian large white breed on the matching ability. This lets use the best combination to improve performance and avoid unwanted flocks crossbreeds combinations.

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