In Vivo Anti-Inflammatory and Antioxidant Potentials of Ethanolic Flower Extract and Isolated Compound, Rutin from Bauhinia tomentosa L.

Bauhinia tomentosa L. belonging to the family Caesalpiniaceae is a traditional medicinal plant species used by the local healers for the treatment of inflammation and other ailments. The present study was addressed to isolate and characterize bioactive entities exists in the ethanolic extract of B. tomentosa flower. Moreover, the anti-inflammatory effects of crude and isolated compounds were assessed using carrageenan induced paw oedema model. Indomethacin, a renowned reference drug was used for comparison. In vivo antioxidant activities were achieved using standard procedures. In anti-inflammatory studies, the isolated compound, rutin (10mg/kgb.w.) manifested potent activity by inhibiting paw oedema (92.6%) than the crude extracts (88.7%). With reference to these findings, rutin also registered eminent therapeutic property in terms of in vivo antioxidant activity. Therefore, the current study exposed that the anti-inflammatory effects of isolated compound, rutin is more expected to depend on their ability to inhibit the production of proinflammatory mediators and proposes that the studied plant species can be deliberated as one of the finest, versatile and most frequently used household remedies for varied manifestations. Furthermore, the compound isolated may serve as a lead molecule for the formulation of active novel drugs without any adverse side effects.

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Biological Evaluation and Docking Analysis of Daturaolone as Potential Cyclooxygenase Inhibitor

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/4098686 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Abdur Rauf ◽

Francesco Maione ◽

Ghias Uddin ◽

Muslim Raza ◽

Bina S. Siddiqui ◽

...

Keyword(s):

In Silico ◽

Biological Evaluation ◽

Inflammatory Activity ◽

Active Constituent ◽

Ligand Complex ◽

Reference Drug ◽

Paw Oedema ◽

Anti Inflammatory ◽

Dose Dependent

This study deals with the isolation of the active constituent(s) from a methanolic extract ofPistacia integerrimaJ. L. Stewart barks and it was also oriented to evaluate thein vivoandin silicoanti-inflammatory activity. By NMR and crystallography techniques, we have isolated a triterpenoid identified as daturaolone (compound1). This compound showedin vivoa significant and dose dependent (1–30 mg/kg) anti-inflammatory activity on carrageenan-induced mouse paw oedema (ED50= 10.1 mg/kg) and on acetic acid-induced writhing responses in mice (ED50= 13.8 mg/kg). In thein vivoexperiments, the effect of tested compound was also evaluated in presence of the reference drug diclofenac (1–30 mg/kg). Moreover,in silicoanalysis of receptor ligand complex shows that compound1interacts with cyclooxygenases (COXs) binding sites displaying an interesting interaction with COX-1. These findings suggest that compound1isolated fromP.integerrimapossessesin vivoanti-inflammatory and antinociceptive potentials, which are supportedin silicoby an interaction with COXs receptors.

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Further Sesquiterpene Lactones from Viguiera robusta and the Potential Anti-Inflammatory Activity of a Heliangolide: Inhibition of Human Neutrophil Elastase Release

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-7-811 ◽

2008 ◽

Vol 63 (7-8) ◽

pp. 533-538 ◽

Cited By ~ 17

Author(s):

Nilton S. Arakawa ◽

Karin Schorr ◽

Sérgio R. Ambrósio ◽

Irmgard Merfort ◽

Fernando B. Da Costa

Keyword(s):

Neutrophil Elastase ◽

Human Neutrophil ◽

Sesquiterpene Lactones ◽

Human Neutrophil Elastase ◽

Reference Drug ◽

Aggregation Factor ◽

Anti Inflammatory ◽

Isolated Compound

In addition to known heliangolides, a new eudesmanolide was isolated from the leaf rinse extract of Viguiera robusta (Asteraceae). Structural elucidation was based on spectral analysis. It is the first report on eudesmanolides in members of the subgenus Calanticaria of Viguiera. In this work, the main isolated compound, the furanoheliangolide budlein A, besides its previously reported in vitro and in vivo anti-inflammatory activities, inhibited human neutrophil elastase release. The inhibition was at the concentration of (16.83± 1.96) μm for formylated bacterial tripeptide (fMLP) stimulation and (11.84±1.62) μm for platelet aggregation factor (PAF) stimulation, being slightly less active than the reference drug parthenolide. The results are important to demonstrate the potential anti-inflammatory activities of sesquiterpene lactones and corroborate the previous studies using other targets.

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Kinetic Study of the Hydrolysis of synthesized Ibuprofen Ester and its Biological Activity

Baghdad Science Journal ◽

10.21123/bsj.10.3.1062-1070 ◽

2013 ◽

Vol 10 (3) ◽

pp. 1062-1070

Author(s):

Baghdad Science Journal

Keyword(s):

Side Effect ◽

Effect Study ◽

Reference Drug ◽

Paw Oedema ◽

Anti Inflammatory ◽

The Stability ◽

Kinetic Hydrolysis ◽

Hydrolysis Of ◽

Inflammatory Action

It is known that the oral administration of ibuprofen caused an irritation of stomach as a side effect due to its carboxylic moiety. Ibuprofen ester was synthesized by linking the carboxylic moiety of ibuprofen and the hydroxylic group of paracetamol to reduce its side effect. Study the kinetic hydrolysis of prepared ester was examined at different values of physiological pH (1.0, 5.8, 6.4 and 7.4) at 37 ± 0.1 of 1 hour period. Measurements of absorbance were carried out by UV-Visible spectrophotometer to follow the stability of ester, it showed Pseudo first order hydrolysis. The pH- apparent rate profiles of ester was exhibited a good stability at pH 1.0 and pH 5.8. Pharmacological activity in vivo of prepared ester was evaluated in relation to analgesic and anti-inflammatory activity using the acetic acid method and the hind paw oedema inhibition, respectively. Acetyl salicylic acid (aspirin) was used as a reference drug for the above tests. The synthesized ester showed higher analgesic and anti-inflammatory action than aspirin.

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BIOACTIVITIES OF GUETTARDA SPECIOSA L. EXTRACTS

Acta Scientifica Malaysia ◽

10.26480/asm.01.2021.20.22 ◽

2020 ◽

Vol 5 (1) ◽

pp. 20-22

Author(s):

Saravanan Vivekanandarajah Sathasivampillai ◽

Moon Jain ◽

Pholtan Rajeev Sebastian Rajamanoharan

Keyword(s):

Systematic Review ◽

Plant Species ◽

Web Of Science ◽

Antioxidant Activities ◽

Bioactive Compound ◽

Scientific Data ◽

Anti Inflammatory

Guettarda speciosa L. is a tree of the Rubiaceae family. G. specious has been used to treat diarrhea, abscesses, wounds, epilepsy, and malaria in ethnomedicinal systems. So far, there is no systematic review for the bioactivities of G. speciosa. Therefore, the purpose of this minireview is to analyze, summarize and document the reported bioactivities of G. speciosa. Major electronic databases (Web of Science, Scopus, ScienceDirect, PubMed, and Semantic Scholar) were used to identify related studies from 1900 to January 2021. So far, in vivo and in vitro scientific data are available for the bioactivities of this plant species. Studies have reported that G. speciosa contains antidiarrheal, antiepileptic, anti-inflammatory, anti-ulcer, antimalarial, antineurodegenerative, and antioxidant activities. Furthermore, no bioactive compound of G. speciosa has been isolated. This work will be useful for researchers interested in bioactivity and phytochemical researches.

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Evaluation of anti-inflammatory and antioxidant activities of Sedum sediforme extracts

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i10.14 ◽

2020 ◽

Vol 19 (10) ◽

pp. 2109-2114

Author(s):

Hayat Trabsa ◽

Imane Krach ◽

Naouel Boussoualim ◽

Soraya Ouhida ◽

Lekhmici Arrar ◽

...

Keyword(s):

Ethyl Acetate ◽

Antioxidant Activities ◽

Chloroform Extract ◽

Scavenging Activity ◽

Reference Drug ◽

Ear Edema ◽

Half Maximal Effective Concentration ◽

Anti Inflammatory

Purpose: To evaluate the in vivo anti-inflammatory effect and in vitro antioxidant activity of the areal part of Sedum sediforme (Jacq.) extracts.Methods: The plant was extracted with solvents of varying polarity (Methanol, chloroform and ethyl acetate, respectively) allowed its separation into three sub-fractions: crude extract, chloroform extract and ethyl acetate extract (CrE, ChE and EaE, respectively). Total polyphenol contents of the extracts were determined. The phorbolmyristate acetate (PMA) induced mice ear edema method was using to evaluate the anti-inflammatory activity. The in vitro scavenging activity was evaluated using enzymatic and non-enzymatic methods. Chelating ability of extracts was assessed using Fe2+–ferrozine complex.Results: The highest content of phenolics compound was in EaE. The administration of CrE (12.5 and 25 mg/kg) reduced ear edema induced by PMA (%I = 35.81 ± 3.18 % and 38.57 ± 2.80 %, respectively), the effect was comparable with that of diclofenac used as a reference drug (%I = 38.84 ± 1.87 %). The in vitro scavenging activity of S. sediforme extracts confirmed that the CrE has the highest enzymatic and non-enzymatic activity with a half maximal inhibitory concentration (IC50) = 0.063 ± 0.005 mg/mL and 0.178 ± 0.006 mg/mL, respectively. However, the ChE present an excellent chelating activity with a half-maximal effective concentration (EC50) of 0.397 ± 0.001 mg/mL.Conclusion: The results show that S. sediforme extracts have a strong antioxidant and antiinflammatory activities which lend some support their use in the traditional medicine. Keywords: Sedum sediforme, Anti-inflammatory, Superoxide scavenger, Cytochrome C, Metal chelating

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Aqueous and Methanol Extracts of Paullinia pinnata (Sapindaceae) Improve Monosodium Urate-Induced Gouty Arthritis in Rat: Analgesic, Anti-Inflammatory, and Antioxidant Effects

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/5946291 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Pius Pum Tseuguem ◽

Télesphore Benoît Nguelefack ◽

Basile Ngnanmegne Piégang ◽

Sorelle Mbankou Ngassam

Keyword(s):

Antioxidant Activities ◽

Gouty Arthritis ◽

Antioxidant Properties ◽

Monosodium Urate ◽

Leaf Extracts ◽

Sod Activity ◽

Reference Drug ◽

Anti Inflammatory ◽

The Brain

The profound modification of lifestyle and food habits has led to an important increase in the prevalence of gout. Unfortunately, there are current unmet needs for the treatment of this disease, prompting the search for new alternatives. Paullinia pinnata is a plant used to treat various diseases including arthritis. The present work aimed to investigate the antigouty activities of the aqueous (AEPP) and methanolic (MEPP) extracts of P. pinnata as well as their in vivo antioxidant properties. The gouty arthritis was induced by injecting 50 μl of monosodium urate (MSU, 100 mg/ml) in the left hind ankle of rats. P. pinnata extracts were administered orally at the doses of 100 and 200 mg/kg/day for 6 days, starting 24 h after MSU injection. Allopurinol 5 mg/kg/day was used as reference drug. Inflammation and hyperalgesia were daily monitored from 24 hours after treatment initiation and for the 6 consecutive days. Myeloperoxidase (MPO) quantification was done in collected synovial fluid. Nitrite oxide (NO), malondialdehyde (MDA), and superoxide dismutase (SOD) were evaluated in the spinal cord and the brain. The serum content of SOD was additionally quantified. AEPP and MEPP significantly (p<0.001) reduce MSU-induced inflammation (22.41% to 93.65%) and hyperalgesia (33.33% to 64.44%) in both ankle and paw. AEPP and MEPP significantly (p<0.001) reduce synovial MPO production with the percentage ranging from 76.30% to 85.19%. AEPP and MEPP significantly (p < 0.001) reduce serum, spinal, left and right hemispheres NO, and MDA and increase the SOD activity (p<0.001). P. pinnata leaf extracts possess potent curative effects against MSU-induced gouty arthritis that combines analgesic, anti-inflammatory, and antioxidant activities. These findings support the use of P. pinnata leaves extracts in the treatment of gouty arthritis and further present the plant as a potent source of efficient antigouty medicine.

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Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf extracts of Andrographis paniculata in some laboratory animals

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2014-0051 ◽

2015 ◽

Vol 26 (4) ◽

Cited By ~ 5

Author(s):

Adeolu Alex Adedapo ◽

Bisi Olajumoke Adeoye ◽

Margaret Oluwatoyin Sofidiya ◽

Ademola Adetokunbo Oyagbemi

Keyword(s):

Acute Toxicity ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Ethanolic Extract ◽

Laboratory Animals ◽

Leaf Extracts ◽

Writhing Test ◽

Paw Oedema ◽

Anti Inflammatory ◽

High Doses

AbstractThe study was designed to evaluate the anti-inflammatory, analgesic and antioxidant properties ofThe dried and powdered leaves of the plant were subjected to phytochemical and proximate analyses. Its mineral content was also determined. Acute toxicity experiments were first performed to determine a safe dose level. The plant material was extracted using water and ethanol as solvents. These extracts were then used to test for the anti-inflammatory, analgesic and antioxidant properties of the plant. The anti-inflammatory tests included carrageenan-induced and histamine-induced paw oedema. The analgesic tests conducted were formalin paw lick test and acetic acid writhing test. The antioxidant activities of the extracts ofThe acute toxicity experiment demonstrated that the plant is safe at high doses even at 1600 mg/kg. It was observed that the ethanolic extract ofThe results from this study may have justified the plant’s folkloric use for medicinal purpose.

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Anti-Inflammatory Activity of the Stem Bark Methanol Extract of Picralima nitida

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i130360 ◽

2021 ◽

pp. 22-28

Author(s):

O. V. Ikpeazu ◽

M. I. Ezeja ◽

K. K. Igwe

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Stem Bark ◽

Inflammatory Activity ◽

Reference Drug ◽

Egg Albumin ◽

Paw Oedema ◽

Anti Inflammatory

Aims: To investigate the anti-inflammatory activity of Picralima nitida stem bark methanol extract (PNSBE). Study Design: The study was carried out using in vivo (carrageenan-induced paw oedema and egg albumin-induced paw oedema) and in vitro (Human red blood cell (HRBC) membrane stabilization assay) models in rat. Place and Duration of Study: Department of Veterinary Physiology and Pharmacology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria from March to July, 2020. Methodology: The extract was used at the doses of 100, 200 and 400 mg/kg while diclofenac (20 mg/kg) was used as the standard reference drug for the in vivo study (carrageenan-induced paw oedema and egg albumin). For the in vitro study (red blood cell haemolysis), the extract was used at the concentrations of 25, 50, 100, 200 and 400 µg/ml while diclofenac 250 µg/ml was used. Results: In the carrageenan-induced paw oedema model, the extract at the doses used and the reference drug significantly (p < 0.05) reduced the paw edema in the rats at 1 hour in a dose dependent manner. Also, in the egg albumin model, the paw oedema of treated rats was significantly (P < 0.05) reduced by PNSBE dose dependently reducing the mean rat paw oedema from 0.99 ± 0.12 to 0.61 ± 0.06 at the first hour. There was also a concentration dependent inhibition of red blood cell haemolysis by the extract in the HRBC membrane stabilization assay. Conclusion: Picralma nitida demonstrated a significant anti-inflammatory activity in this study.

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Fibrinolytic, Anti-Inflammatory and Cytotoxic Potentialities of Extracts and Chemical Constituents of Manglicolous Lichen, Graphis ajarekarii Patw. & C. R. Kulk

The Natural Products Journal ◽

10.2174/2210315508666180604101813 ◽

2020 ◽

Vol 10 (1) ◽

pp. 87-93 ◽

Cited By ~ 1

Author(s):

Vinay Bharadwaj Tatipamula ◽

Girija Sastry Vedula

Keyword(s):

Protein Denaturation ◽

Biological Activities ◽

Chemical Constituents ◽

Fibrin Clot ◽

Hydroalcoholic Extract ◽

Paw Oedema ◽

Anti Inflammatory ◽

Rat Paw ◽

Rat Paw Oedema

Background: Lichens which are betide to mangroves are termed as Manglicolous Lichens (ML). As these ML are habituated under stress conditions, they are screened for unique metabolites and biological activities. Objective: The study aimed to establish the chemical and biological profile of ML, Graphis ajarekarii. Methods: The Ethyl Acetate Extract of G. ajarekarii (EAE) was subjected to chromatographic techniques and the obtained isolates were characterized by spectroscopic analysis. The hydroalcoholic extract of G. ajarekarii (AE), EAE, isolates and Hydroalcoholic Extract of host (HE) were evaluated for fibrinolytic (fibrin clot method), in vitro (protein denaturation method) and in vivo (formalin-induced rat paw oedema assay), anti-inflammatory and cytotoxicity (MTT assay) activities. Results: Chemical investigation of the EAE led to the isolation of two known compounds namely atranorin (1) and ribenone (2), which were confirmed by spectral data. The AE and EAE gradually lysed the fibrin clot with 94.54 and 65.07%, respectively, at 24 h. The AE inhibited protein denaturation of about 88.06%, while the standard (Indomethacin) with 93.62%. Similarly, the in vivo antiinflammatory analysis of AE (200 mg/mL) showed potent reduction of rat paw oedema than the standard, whereas EAE and 1 depicted moderate depletion. In addition, the AE revealed prominence inhibition on MCF-7, DU145 and K-562 with IC50 values of 69.5, 42.5 and 38 µg/mL, respectively, whereas the HE exhibited mild inhibitory profile against fibrin clot, inflammation and cancer. Conclusion: From the results, it can be concluded that the G. ajarekarii has an aptitude to act against coagulation, inflammation and cancer cells.

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Periplaneta americana Oligosaccharides Exert Anti-Inflammatory Activity through Immunoregulation and Modulation of Gut Microbiota in Acute Colitis Mice Model

Molecules ◽

10.3390/molecules26061718 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1718

Author(s):

Kaimin Lu ◽

Jing Zhou ◽

Jie Deng ◽

Yangjun Li ◽

Chuanfang Wu ◽

...

Keyword(s):

Side Effects ◽

Pathogenic Bacteria ◽

Periplaneta Americana ◽

Biochemical Characterization ◽

Antioxidant Activities ◽

Inflammatory Activity ◽

Mice Model ◽

Acute Colitis ◽

Anti Inflammatory

The incidence and prevalence of inflammatory bowel disorders (IBD) are increasing around the world due to bacterial infection, abnormal immune response, etc. The conventional medicines for IBD treatment possess serious side effects. Periplaneta americana (P. americana), a traditional Chinese medicine, has been used to treat arthritis, fever, aches, inflammation, and other diseases. This study aimed to evaluate the anti-inflammatory effects of oligosaccharides from P. Americana (OPA) and its possible mechanisms in vivo. OPA were purified and biochemical characterization was analyzed by HPGPC, HPLC, FT-IR, and GC–MS. Acute colitis mice model was established, the acute toxicity and anti-inflammatory activity were tested in vivo. The results showed OPA with molecular mass of 1.0 kDa were composed of 83% glucose, 6% galactose, 11% xylose, and the backbone was (1→4)-Glcp. OPA had potent antioxidant activities in vitro and significantly alleviated the clinical symptoms of colitis, relieved colon damage without toxic side effects in vivo. OPA exhibited anti-inflammatory activity by regulating Th1/Th2, reducing oxidative stress, preserving intestinal barrier integrity, and inhibiting TLR4/MAPK/NF-κB pathway. Moreover, OPA protected gut by increasing microbial diversity and beneficial bacteria, and reducing pathogenic bacteria in feces. OPA might be the candidate of complementary and alternative medicines of IBD with low-cost and high safety.

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