scholarly journals Acute Toxicity of NH4Cl to Tambra Fish (Tor Tambroides) Fry

2021 ◽  
Vol 2 (2) ◽  
pp. 49-55
Author(s):  
Gunawan Pratama Yoga ◽  
Kristin Noni Riyanti Buraen ◽  
Sutanto Sutanto

Ammonia is one of the nutrient wastes products which can be toxic to all vertebrates, including fish. Usually, ammonia toxicity is expressed as Total Ammonia Nitrogen (TAN, mgN/L). Tambra fish (Tor tambroides) is freshwater fish with important economic value in some areas in Indonesia. According to overfishing and forest degradation, the fish has become threatened. This research aimed to calculate the LC50 value of ammonia to Tambra fish (Tor tambroides) and estimate  NOEC,  LOEC, and MATC values of ammonia to Tambra fish (Tor tambroides). This study was conducted using the static test method with two steps. The first stage is a preliminary test, where the preliminary test is observed for 24 hours With five concentrations excluding control. Whereas in the second stage that is carried out, observation for 96 hours with four concentrations excluding control. The Probit test is Performed to obtain LC50 value results, then proceed with ammonia analysis using the Salicylate Test Kit method. The results showed that ammonia had acute toxicity with LC50-96 hour value of NH4Cl against Tambra fish fry (Tor tambroides) in the amount of 354.615 mg/L. Ammonia caused acute toxicity to Tambra fish fry (Tor tambroides).The LC50-96 hour value was 354.615 mg/L in the form of Total Ammonia Nitrogen (TAN) or it was equivalent to un-ionized Ammonia (NH3) of 9.8937 mg/L, while values of the Lowest Observed Effect Concentration (LOEC) and No Observed Effect Concentration (NOEC) of ammonia on Tambra fish (Tor tambroides) were 578.24 mg/L and 280.18 mg/L, respectively.

2016 ◽  
Vol 74 (4) ◽  
pp. 935-942 ◽  
Author(s):  
Seyong Park ◽  
Fenghao Cui ◽  
Kyung Mo ◽  
Moonil Kim

In this study, we evaluated ammonia toxicity in mesophilic anaerobic digestion at various pH values and total ammonia nitrogen (TAN) concentrations. We performed anaerobic toxicity assays (ATAs) to evaluate the toxicity effects of TAN and pH on mesophilic anaerobic digestion. Modeling based on the results of the ATAs indicated that the specific methanogenic activity (SMA) decreased by 30% at a TAN concentration higher than 3.0 g/L compared to a TAN concentration of 0 g/L. In addition, the highest SMA for a given TAN level (0.5–10.0 g/L) was observed at a pH of around 7.6. The results of bacterial community analyses showed that the diversity and richness of microorganisms with increasing TAN concentration were decreased. Chloroflexi and Synergistetes were the dominant phyla at TAN concentrations less than 3.0 g/L, and Firmicutes was the dominant phylum at TAN concentrations higher than 3.0 g/L, implying that the ammonia toxicity concentration may influence the kind of dominant species. In conclusion, to start a stable mesophilic anaerobic digestion concerning ammonia toxicity, a TAN concentration less than 3.0 g/L is preferable.


2020 ◽  
Vol 1 (5) ◽  
pp. 941-948
Author(s):  
Ambar Fidyasari ◽  
Sentot Joko Raharjo ◽  
Melani Setyowati

Soursop fruit (Annona montana Macf.) is one of the plants can be used as as traditional medicine. This plant contains terpenoid and acetogenin which can cause toxicity. The fruit has a flavor that is tasteless so the innovation becomes probiotic drinks. This drink has been proven as an antioxidant, antibacterial, antihyperuricemia and antidiarrheal. The aim of this study was to know about acute toxicity of probiotic drink of soursop juice using brine shrimp lethality test method which will be indicated by LC50 value. This study used experimental methods conducted in the Laboratory of Farmakoknosi. There are several variations in concentration in this study, namely 10000 ppm, 20000 ppm, 30000 ppm, 40000 ppm, 50000 ppm, 60000 ppm, 70000 ppm, 80000 ppm and replication was done 3 times with total number of test animals used was 270. The results showed that probiotic drink of soursop juice can provide acute toxic effects on test animals with LC50 value of 29717,23 ppm. LC50 values ​​indicate that the mountain soursop probiotic drink is not potentially toxic because it has a value of >1000 ppm.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Bharat Nandkumar Mulay ◽  
Konda Rajasekhar Reddy

Abstract This experimental investigation of a laboratory scale aquaponic system included testing of a biofilter with basil plant as the biofilter part on aquaculture water quality. Irridescent shark was selected as aquaculture species. The biofilter consist of natural biomaterials such as coconut husk, coco peat, and coarse aggregates. The experiment was carried out for three short water recirculation durations of 2, 3, and 4 h/day. The influent and effluent ammonia NH3, total ammonia nitrogen (TAN), nitrites NO2 and nitrates NO3 levels were measured and analysed. The results showed that the biofilter effectively removed NH3 (65 - 71 %), TAN (34 - 58 %), and NO2 (60 - 67 %) from the aquaculture water. The dissolved oxygen (DO) levels were maintained between 3 - 7.0 mg/l during all the recirculation durations. The significance of water recirculation period was assessed by calculating the differences between the means of water quality parameters with a statistical test named one-way analysis of variance (ANNOVA) with significant level P taken as 5 %, i.e., P ≤ 0.5. The effluent mean NH3 levels 0.030 mg/l, 0.033 mg/l, and 0.022 mg/l exhibited significant difference at 4 h/d periods while effluent TAN levels 0.81, 0.77, and 0.77 showed no difference with varying periods.


Aquaculture ◽  
2020 ◽  
Vol 520 ◽  
pp. 734963 ◽  
Author(s):  
Mamoru Oshiki ◽  
Takashi Aizuka ◽  
Hirotoshi Netsu ◽  
Satoshi Oomori ◽  
Akihiro Nagano ◽  
...  

2020 ◽  
Vol 412 (13) ◽  
pp. 3167-3176 ◽  
Author(s):  
Yanisa Thepchuay ◽  
Raquel B. R. Mesquita ◽  
Duangjai Nacapricha ◽  
António O. S. S. Rangel

2017 ◽  
Vol 39 (5) ◽  
pp. 601
Author(s):  
Davi De Holanda Cavalcante ◽  
Francisco Roberto Dos Santos Lima ◽  
Vanessa Tomaz Rebouças ◽  
Marcelo Vinícius do Carmo e Sá

The present study aimed to assess the possible beneficial effects of the integration between bioflocs and periphyton to the Nile tilapia’s water quality and growth performance. There were four treatments with five replicates each: (1) Control: green waters, (2) Periphyton: substrate-based system, (3) BFT: bioflocs technology for aquaculture, and (4) Biophyton: integration between bioflocs and periphyton. Fish (1.63 ± 0.07 g) were reared for 10 weeks in twenty 250 L outdoor tanks. Two polyethylene boards were vertically set out in the Periphyton and Biophyton tanks as underwater substrates. The C: N ratios of water in the BFT and Biophyton tanks were adjusted to 15:1 with dry molasses applications. The concentrations of total ammonia nitrogen were higher in the Control and Periphyton tanks than in the BFT and Biophyton ones. On the other hand, the concentrations of reactive phosphorus were higher in the BFT and Biophyton tanks than in the Control and Periphyton ones. The fish final body weight, specific growth rate and fish yield have not differed between the tanks. The integration between bioflocs and periphyton has not brought clear benefits to tilapia culture on water quality and growth performance.  


2013 ◽  
Vol 35 (4) ◽  
Author(s):  
Francisco Jackes Rodrigues da Silva ◽  
Francisco Roberto dos Santos Lima ◽  
Diego Alves do Vale ◽  
Marcelo Vinícius do Carmo e Sá

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