The impact of the nucleotide-binding oligomerization domain 1 (NOD 1) gene polymorphism on Helicobacter Pylori induced chronic gastritis in hepatic patients.

2021 ◽  
Vol 30 (3) ◽  
pp. 9-19
Author(s):  
Enas M. Ghoneim ◽  
Eman H. Hassan ◽  
Hassan Zaghla ◽  
Doha Taie ◽  
Samah M. Awad
Keyword(s):  
Helicobacter Pylori ◽  
Gene Polymorphism ◽  
Chronic Gastritis ◽  
Nucleotide Binding ◽  
Esophageal Hiatus ◽  
Rapid Urease Test ◽  
Tissue Samples ◽  
Urease Test ◽  
The Impact ◽  
Oligomerization Domain

Background: Chronic infection with Helicobacter pylori (H.pylori ) causes atrophic and even gastric metaplastic changes, and it has a well-known link to peptic ulceration. Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is a protein receptor that is presented by the NOD1 gene. It distinguishes H.pylori bacterial molecules and enhances an immune response Objectives: to describe the relation between the NOD1 gene (rs2075820) polymorphism and H.pylori infection in hepatic and non hepatic patients with chronic gastritis, study its impact on the degree of chronic gastritis in H.pylori positive individuals, and to examine the effect of H. pylori on clinical, endoscopic and histopathological findings and child paugh scoring in hepatic patients. Methodology: Gastric tissue samples were taken from selected 200 patients with chronic gastritis, either hepatic or non hepatic. Rapid urease test and pathological findings classified them into H.pylori infected and non infected patients. Genotyping of NOD 1 was studied using polymerase chain reaction /restriction fragment length polymorphism (PCR–RFLP) method. Results: A significant higher frequency of AA genotype, and the A allele of NOD1 gene in H.pylori +ve patients, either hepatic; (58%)-(73%) or non hepatic;(62%)-(78%) as compared to H.pylori –ve patients,(P <0.001). A highly significant relation between NOD1 genotypes and endoscopic findings, where most of H.pylori infected patients with AA genotype had more peptic ulcer, antral erosion, gastric prolapse, esophageal varices and esophageal hiatus hernia compared to patients with GA and GG genotypes, (P<0.001). No significant impact of H.pylori on signs of liver affection and child paugh scoring in hepatic patients. Conclusions: In NOD1 gene polymorphism, AA genotype and A allele have significantly implicated in H.pylori infection susceptibility and progression. While GG genotype and G allele have a protective effect against H.pylori infection.

scholarly journals Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

2007 ◽  
Vol 56 (8) ◽  
pp. 1081-1085 ◽  
Author(s):  
Lyudmila Boyanova ◽  
Elena Lazarova ◽  
Christo Jelev ◽  
Galina Gergova ◽  
Ivan Mitov
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Living Environment ◽  
Rural Children ◽  
Rapid Urease Test ◽  
Infection Prevalence ◽  
Helicobacter Heilmannii ◽  
Urease Test ◽  
H Pylori ◽  
The Impact

The aims of the study were to evaluate the incidence of Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children from 1996 to 2006, to analyse the performance of diagnostic tests, and to look at H. pylori density in specimens by culture. Antral specimens from children with chronic gastritis (n=513), peptic ulcers (n=54) and other diseases (n=91) were evaluated by direct Gram staining (DGS), in-house rapid urease test (RUT) and culture. The living environment and semi-quantitative H. pylori density were assessed in 188 and 328 children, respectively. H. pylori infection was found in children with ulcers (77.8  %), chronic gastritis (64.5  %) and other diseases (36.3  %). Half (51.4  %) of patients aged 1–5 years and 77.4  % of those aged 16–17 years were H. pylori-positive. Of all children, 328 (49.8  %) showed positive DGS, 184 (28  %) had a positive RUT, and 386 (58.7  %) were culture-positive. Unlike gastric mucus specimens, frozen biopsy specimens provided reliable diagnosis. H. heilmannii was observed in two (0.3  %) children. High H. pylori density (growth into all quadrants of plates) was found in 18  % of 328 children evaluated, involving 31  % of ulcer and 16.7  % of non-ulcer patients. H. pylori infection was more common in rural children with chronic gastritis (91.3  %) than in the remainder (66.7  %). In conclusion, H. pylori infection was common in symptomatic Bulgarian children. The infection prevalence was >77  % in patients aged 16–17 years, in children with a duodenal ulcer, and in rural patients. H. heilmannii infection was uncommon. The performance of the bacterial culture was good. The impact of H. pylori density on the clinical expression and eradication of the infection requires further evaluation. The results highlight the need for routine H. pylori diagnosis in rural children with chronic gastritis.

scholarly journals Detection of Helicobacter pylori Through Histochemistry & Immunofluorescent Staining in Biopsies of Patients with Chronic Gastritis

2011 ◽  
Vol 34 (4) ◽  
pp. 4-9
Author(s):  
Asia Jahanzeb ◽  
Abdul Hannan Nagi Nagi ◽  
Ayesha Suleman
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Histopathological Examination ◽  
Endoscopic Examination ◽  
Immunofluorescent Staining ◽  
Rapid Urease Test ◽  
Specific Method ◽  
Total N ◽  
Urease Test ◽  
Gastric Biopsies

Introduction: Helicobacter pylori (H.pylori) is a helix shaped gram negative rod which is usually associated with chronic gastritis and also a major cause of other astroduodenal diseases as well. Aims & Objectives: The present study used histochemical and immunofluorescent stains on formalin fixed paraffin embedded human gastric biopsies for detection of H.pylori. Comparison was also done to evaluate best staining method. Place and duration of study: This study was accomplished in about one year. Sampling of gastric biopsies and rapid urease test were executed at the endoscopy suite of Lahore General Hospital whereas the histopathological examination and immunofluorescent staining were done in University of Health Sciences, Lahore. Material & Methods: Thirty patients (n=30cases) were included in the study following inclusion criteria. Diagnostic upper GI endoscopy was carried out in all cases. Five gastric biopsies were taken from each patient/case (total N=150 Biopsies) according to the Updated Sydney System. Rapid urease test was performed at the site of endoscopy. Biopsies fixed in 10% formalin were brought to the concerned department where they were assigned a specific laboratory number then processed and stained. Results: Endoscopic examination revealed chronic gastritis and rapid urease tests were positive. All cases (n=30) were positive for H.pylori on histopathology. The calculated sensitivity and specificity of H&E, Giemsa, Modified McMullen’s stain and Immunofluorescent method in present study were 71% and 100%,83% and 100%, 82% and 100%, 90% and 100% respectively. Conclusion: Special stains makes H.pylori identification easier in tissue sections. However, immunofluorescent test is the most sensitive and specific method as compared to histochemical stains.

scholarly journals The impact of Helicobacter pylori resistance on the efficacy of a short course pantoprazole based triple therapy

2003 ◽  
Vol 40 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Jaime Natan Eisig ◽  
Suraia Boaventura André ◽  
Fernando Marcuz Silva ◽  
Cláudio Hashimoto ◽  
Joaquim Prado Pinto Moraes-Filho ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antimicrobial Resistance ◽  
Triple Therapy ◽  
Treatment Success ◽  
Rapid Urease Test ◽  
Disk Diffusion Test ◽  
Ulcer Disease ◽  
Short Course ◽  
Urease Test ◽  
The Impact

BACKGROUND: Many of the currently used Helicobacter pylori eradication regimens fail to cure the infection due to either antimicrobial resistance or poor patient compliance. Those patients will remain at risk of developing potentially severe complications of peptic ulcer disease. AIM: We studied the impact of the antimicrobial resistance on the efficacy of a short course pantoprazole based triple therapy in a single-center pilot study. METHODS: Forty previously untreated adult patients (age range 20 to 75 years, 14 males) infected with Helicobacter pylori and with inactive or healing duodenal ulcer disease were assigned in this open cohort study to 1 week twice daily treatment with pantoprazole 40 mg, plus clarithromycin 250 mg and metronidazole 400 mg. Helicobacter pylori was assessed at entry and 50 ± 3 days after the end of treatment by rapid urease test, culture and histology of gastric biopsies. The criteria for eradication was a negative result in the tests. Susceptibility of Helicobacter pylori to clarithromycin and metronidazole was determined before treatment with the disk diffusion test. RESULTS: One week treatment and follow up were complete in all patients. Eradication of Helicobacter pylori was achieved in 35/40 patients (87.5%) and was higher in patients with nitroimidazole-susceptible strains [susceptible: 20/20 (100%), resistant: 10/15 (67%)]. There were six (15%) mild adverse events reports. CONCLUSIONS: A short course of pantoprazole-based triple therapy is well tolerated and effective in eradicating Helicobacter pylori. The baseline metronidazole resistance may be a significant limiting factor in treatment success.

EFFICACY OF RACM REGIMEN ON HELICOBACTER PYLORI ERADICATION IN PATIENTS OF CHRONIC GASTRITIS

2016 ◽  
pp. 88-93
Author(s):  
Thi Hoai Thai ◽  
Van Huy Tran
Keyword(s):  
Helicobacter Pylori ◽  
Side Effects ◽  
Chronic Gastritis ◽  
Eradication Rate ◽  
Statistical Significance ◽  
Rapid Urease Test ◽  
Antibiotic Resistant ◽  
Helicobacter Pylori Eradication ◽  
Urease Test ◽  
H Pylori

Background: H. pylori eradication still remains a challenge to clinicians, especially with the increasing antibiotic-resistant H. pylori. Concomitant therapy showed effective, even in some multiresistant population, but data in Vietnam is still very limited. The study ''Study of Helicobacter pylori eradication with RACM regimen in chronic gastritis patients at Da Nang Hospital from 1/4/2014 to 30/6/2015, is aimed at: (1) Evaluating the results of Helicobacter pylori eradication of Amoxicillin-Clarithromycin-Rabeprazole-Metronidazole therapy for 14 days.(2) Assessing some side effects of this regimen.Method: prospective, consisting of 83 patients examined and treated in Danang hospital from1/ 4/2014 to 30/6/2015, H.pylori was tested by rapid Urease test; H.pylori positive patients received RACM for 14 days. Results: H.pylori eradication rate was 83.1%. H. pylori eradication rates in different locations: antrum 63.8%, higher than corpus (17.4%), antrum and corpus (18.8%), with statistical significance at p<0.05. Common side effects was nausea (27.7%), diarrhea (19.3%). Abdominal pain, lightheadedness, dizziness, insomnia, headache account for low percentage: 8%; 6%; 3,6% and 2.4% respectively. Conclusion: The effect of 14 day RACM regimen for H. pylori eradication was 83.1%, common side effects are nausea (27.7%), diarrhea (19.3%). Key words: chronic gastritis;H. pylori; eradication of H. pylori(ITT); RACM regimen.

CHRONIC HELICOBACTER PYLORI GASTRITIS: THE ERADICATION EFFICACY&NBSP;OF THE&NBSP;BISMUTH-CONTAINING QUADRUPLE REGIMEN (EBMT)

2016 ◽  
pp. 149-158
Author(s):  
Ngoc Quy Hue Dang ◽  
Van Huy Tran ◽  
Thanh Hai Nguyen
Keyword(s):  
Helicobacter Pylori ◽  
Side Effects ◽  
Chronic Gastritis ◽  
Medication Compliance ◽  
Compliance Rate ◽  
Eradication Therapy ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori ◽  
Helicobacter Pylori Gastritis

Background: there has not been yet any research on the effectiveness of H. pylori eradication of bismuth-containing quadruple regimen on chronic gastritis patients in our country. Objective: to evaluate H. pylori eradication rate of bismuth-containing quadruple regimen according to intention to treat (ITT), per protocol (PP) analysis, the rate of side effects and medication compliance. Subjects and Methods: from March 2014 to January 2016 we used bismuth-containing quadruple regimen (EBMT) 10 days for H. pylori eradication therapy for 166 chronic gastritis patients diagnosed based on clinical, endoscopic, rapid urease test, histology and culture. Patients were evaluated side effects and medication compliance at the end of treatment (day 11-14). To assess the eradication, repeating endoscopy with both rapid urease test and histological examination were performed at 4 to 8 weeks after stopping treatment course. Results: H. pylori eradication rates on ITT and PP analysis overall, for naïve patients, after one and more two eradication failures were respectively: 80.72-89.33%, 79.51-90.65%, 91.67-91.67% and 75.00-78.95%. Medication adherence rate was 96.99%. The rates of patients experiencing moderate, severe and very severe side effects were: 19.88%, 0.60% and 1.81%. Conclusion: the EBMT 10-day regimen attained high eradication rates in chronic gastritis patients with rare serious side effects and the high compliance rate. We should apply bismuth-containing quadruple regimen in H. pylori eradication therapy for naïve patients or after one eradication failure. Key words: bismuth-containing quadruple regimen, EBMT, eradication, chronic Helicobacter pylori gastritis

scholarly journals Dependence of the state of the large intestine parietal microflora on the activity of Helicobacter pylori associated chronic gastritis

2021 ◽  
Vol 20 (3) ◽  
pp. 44-48
Author(s):  
H. B. Isayev ◽  
M. R. Ibrahimov ◽  
T. H. Salimov
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Histological Examination ◽  
Mucous Membrane ◽  
Large Intestine ◽  
Chronic Gastritis ◽  
Rapid Urease Test ◽  
E Coli ◽  
Urease Test ◽  
H Pylori

Introduction. The aim of the study was to detect the dependence of the composition of the parietal microflora of the colon on the activity of Helicobacter pylori-associated chronic gastritis and the degree of Helicobacter pylori contamination of the gastric mucosa. Materials and methods. The study was carried out on 50 patients aged 24 to 72 years, who were diagnosed with HP-associated chronic gastritis in the phase of exacerbation based on the results of FEGDS and histological examination of biopsies of the mucous membrane of the antrum of the stomach in combination with a rapid urease test. A series of dilutions 10¹-10⁹ was prepared from a homogenized biopsy specimen of the mucous membrane of the sigmoid colon in 0.1 ml of isotonic sodium chloride solution, and 0.1 ml of a solution of the corresponding dilution was inoculated onto the surface of the nutrient medium. The study of mucosal microflora was carried out by means of bacteriological examination of biopsies of the mucous membrane of the sigmoid and cecum. The degree of H. pylori contamination of the gastric mucosa was assessed in the course of histological examination by a semi-quantitative method: low degree of contamination — up to 20 microbial bodies, average — 20-50 microbial bodies, strong — over 50 microbial bodies in the field of view. Results. The study demonstrated that the indicators of the average concentrations of bifidobacteria, lactobacilli and typical strains of E. coli progressively decreased with the transition to a higher degree of HP infection. This tendency was maximum in relation to bifidobacteria — 6.1-1.57-1.09 x 10⁸ CFU/g, with a difference between the maximum (I degree) and minimum (III degree) 5.59 times. A similar relationship, but much less pronounced, was observed for Lactobacterium spp. and E. coli. As the severity of HP infiltration of the gastric mucosa increases, there is a decrease in the concentration of the above microorganisms in the parietal mucin - the correlation coefficient is r = -0.74. Discussion. Since the mucous microflora is more stable and functionally significant than the cavity microflora, the data obtained allow us to consider Helicobacter pylori as an etiological factor of colonic dysbiosis. Conclusion. The probability of excessive growth of conditionally pathogenic fraction of intestinal parietal microflora has a direct dependence on the severity of Helicobacter pylori infestation, being maximum at degree III contamination. Content of bifidobacteria, lactobacilli and typical E. coli strains in the intestinal biotope of the large intestine is inversely related to the degree of histological activity of HP-associated gastritis.

STUDY OF HELICOBACTER PYLORI ERADICATON RATE OF BISMUTH-CONTAINING QUADRUPLE REGIMEN IN PATENTS WITH CHRONIC GASTRITS

2016 ◽  
pp. 31-35
Author(s):  
Van Huy Tran ◽  
Thi Minh Trieu Nguyen
Keyword(s):  
Helicobacter Pylori ◽  
Side Effects ◽  
Chronic Gastritis ◽  
Eradication Rate ◽  
High Rate ◽  
Rapid Urease Test ◽  
Good Tolerance ◽  
Central Vietnam ◽  
Urease Test ◽  
H Pylori

Background: Quadruple therapy with bismuth was considered as a promising regimen in the eradication of Helicobacter pylori in the population with clarithromycine- resistant high rate, but data in Vietnam was still limited. This study is aimed at determining the eradication rate of quadruple regimen and its side effects in the patients with chronic gastritis. Patients and methods: 98 patients with chronic gastritis histologically diagnosed and postive with rapid urease test were enrolled. Patients received Rabeprazole 20 mg bid, Pepto bismol 2tab bid, tetracyclin 1000 mg bid and Metronidazole 550 mg bid for 10 days. Results: Eradication rates of quadruple regimens with bismuth were 91.8% in ITT analysis and 94.7% in PP analysis. Rate of compliance was 96.9%. The side effects were minor and transient. Conclusion: Quadruple regimen with bismuth showed a high rate of H. pylori eradication and a good tolerance in patients with chronic gastritis in central Vietnam. Key words: Helicobacter pylori, quadruple regimen, bismuth

A Clinical Significance of Helicobacter Pylori Infection in Hemophilic Children with Gastrointestinal Hemorrhage.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4442-4442
Author(s):  
Ji Yoon Kim ◽  
Yong-Mook Choi ◽  
Kun Soo Lee
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Gastrointestinal Hemorrhage ◽  
Conflicts Of Interest ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Urease Test ◽  
Life Threatening ◽  
H Pylori

Abstract Abstract 4442 Purpose For hemophiliacs gastrointestinal hemorrhage is a life-threatening complication and can be caused by the Helicobacter pylori infection. In this study, the prevalence of H. pylori infection among child hemophiliacs with gastrointestinal hemorrhage and the recurrence rate after eradication H. pylori treatment was investigated. Patients and Methods Seven children with hemophilia A with hematemesis (age, 5.3∼17.0 year) were evaluated for the causes of gastrointestinal hemorrhage and the detection of H. pylori. Gastroendoscopy was done to find the bleeding focus and for further evaluation including rapid urease test and mucosal biopsy. Result: Four patients had dyspepsia and abdominal pain for several weeks or months prior to hematemesis. Three patients did not show any signs of bleeding. From gastroendoscopy, four patients were diagnosed as duodenal ulcer, one as H. pylori associated chronic gastritis and one as hemorrhagic gastritis. One patient showing a normal finding was diagnosed with adenoid hemorrhage after nasopharyngoscopy. H. pylori infection was found in four of six patients with GI bleeding (3, duodenal ulcer; 1, H. pylori associated chronic gastritis). The patients with H. pylori infection had an eradication treatment of triple therapy and no recurrence happened. Conclusion In child hemophiliacs, H. pylori should also be considered as an important cause of gastrointestinal hemorrhage. The recurrence of the infection and gastrointestinal hemorrhage can be prevented with eradication of H. pylori. Screening test for H. pylori would be needed in child hemophiliacs in endemic area. Disclosures: No relevant conflicts of interest to declare.

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