Calreticulin immunohistochemistry in myeloproliferative neoplasms - evolution of a new cost-effective diagnostic tool: a retrospective study with histological and molecular correlation

2021 ◽  
Author(s):  
Sanjeet Roy ◽  
Marie Therese Manipadam ◽  
Poonkuzhali Balasubramanian
Keyword(s):  
Retrospective Study ◽  
Diagnostic Tool ◽  
Myeloproliferative Neoplasms ◽  
Cost Effective ◽  
Molecular Correlation

scholarly journals CRISPR/Cas12a-Based Ultrasensitive and Rapid Detection of JAK2 V617F Somatic Mutation in Myeloproliferative Neoplasms

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 247
Author(s):  
Miaomiao Chen ◽  
Chunhua Zhang ◽  
Zhiqing Hu ◽  
Zhuo Li ◽  
Menglin Li ◽  
...  
Keyword(s):  
Detection System ◽  
Myeloproliferative Neoplasms ◽  
Cost Effective ◽  
Jak2 V617f ◽  
Jak2 V617f Mutation ◽  
Minimum Detectable Concentration ◽  
Lateral Flow Strip ◽  
Whole Process ◽  
Detectable Concentration ◽  
V617f Mutation

The JAK2 V617F mutation is a major diagnostic, therapeutic, and monitoring molecular target of Philadelphia-negative myeloproliferative neoplasms (MPNs). To date, numerous methods of detecting the JAK2 V617F mutation have been reported, but there is no gold-standard diagnostic method for clinical applications. Here, we developed and validated an efficient Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein 12a (Cas12a)-based assay to detect the JAK2 V617F mutation. Our results showed that the sensitivity of the JAK2 V617F/Cas12a fluorescence detection system was as high as 0.01%, and the JAK2 V617F/Cas12a lateral flow strip assay could unambiguously detect as low as 0.5% of the JAK2 V617F mutation, which was much higher than the sensitivity required for clinical application. The minimum detectable concentration of genomic DNA achieved was 0.01 ng/μL (~5 aM, ~3 copies/μL). In addition, the whole process only took about 1.5 h, and the cost of an individual test was much lower than that of the current assays. Thus, our methods can be applied to detect the JAK2 V617F mutation, and they are highly sensitive, rapid, cost-effective, and convenient.

scholarly journals A review of Nadi Parikshan in various Ayurvedic Samhita with its clinical methods

2020 ◽  
Vol 4 (06) ◽  
Author(s):  
Pooja Babaso Kamble
Keyword(s):  
Diagnostic Tool ◽  
Common Factor ◽  
Research Work ◽  
Cost Effective ◽  
The Other ◽  
Diagnostic Tools ◽  
Healthy Individuals ◽  
Medical Field ◽  
Clinical Methods ◽  
Time Region

Nadi Pariksha is the most effective diagnostic tool known in the medical field. It is cost effective,  accurate,  safe,  and gives quick results. We can conduct Nadi Pariksha on healthy individuals as well as all patients irrespective of stage of the disease also,  and even pregnant woman,  children,  elderly can undergo without any harm or side effects. However,  this technique is not being widely practised at present,  because of lack of training,  practise and knowledge about it in the present day among Ayurveda vaidyas. An iconic factor for identification of a physician,  irrespective of the time,  Region,  Nadi Pariksha can be highlighted as a common factor or even System of Medicine or Civilization of the known world. Thus,  we can perceive that Nadi Pariksha or the pulse examination remains as an effective diagnostic tool since ages. Nadi Pariksha was not been discussed among the Brihatrayees of Ayurveda. Acharya Sharangdhara was the first to document in the doctrines of Ayurveda. Thus Acharya Sharangdhara is considered as ‘The Founder of Nadi Pariksha’in Ayurveda. Nadi Pariksha was titled under the Pancha-Nidana by Acharya Sharangdhara and Ashta Sthana Pariksha by Acharya Yogaratnakara. It was the Foremost among all the other diagnostic tools mentioned by him. Later Acharyas like Acharya Bhava Mishra,  Acharya Yogaratnakara,  Acharya Basavaraja,  Acharya Kanada Maharishi,  and Acharya Ravana have contributed in giving more descriptions and importance. In the recent days Dr. Vasant lad and Dr. Sarvadeva Upadhaya’s research work interest and scope of Nadi Pariksha.

scholarly journals Treatment of epistaxis without the use of nasal packing, a patient study

2011 ◽  
Vol 49 (5) ◽  
pp. 600-604
Author(s):  
Esther Vis ◽  
Herbert van den Berge
Keyword(s):  
Retrospective Study ◽  
The Netherlands ◽  
Treatment Modality ◽  
Training Programs ◽  
Cost Effective ◽  
Nasal Packing ◽  
Recurrent Bleeding ◽  
Bleeding Site ◽  
Recurrence Rates ◽  
Patient Study

We questioned how many patients with epistaxis can be treated by cautery without the use of nasal packing, as cautery is more effective and efficient. To investigate this, we performed a retrospective study of a cohort of 418 patients with epistaxis who presented to one ENT consultant at the ENT department of Medisch Centrum Leeuwarden (the Netherlands) between 1997 and 2007. Main outcomes were the treatment modality (cautery and/or nasal packing), recurrence of epistaxis and need for hospitalization. In 98% of all patients the bleeding site could be found and treated by cautery. The incidence of recurrent bleeding was 6%. Two percent of all patients had to be admitted to the hospital. This is considerably lower compared with recurrence rates and hospitalization after treatment by nasal packing known from the literature. Therefore we concluded that nearly all patients can be effectively treated by cautery with a low recurrence rate. In addition, this method of treatment is very cost effective. Because cautery requires skill and appropriate facilities, we recommend special attention for this in ENT training programs.

scholarly journals A retrospective study of endometrial histopathology in abnormal uterine bleeding patients

2018 ◽  
Vol 7 (10) ◽  
pp. 4116
Author(s):  
Neha Varun ◽  
Nidhi Gupta ◽  
Sana Khan
Keyword(s):  
Retrospective Study ◽  
Endometrial Hyperplasia ◽  
Diagnostic Procedure ◽  
Abnormal Uterine Bleeding ◽  
Cost Effective ◽  
Uterine Bleeding ◽  
Age Group ◽  
Medical College ◽  
Different Types ◽  
Proliferative Endometrium

Background: Abnormal uterine bleeding (AUB) is one of the most commonly encountered gynecological problem and almost 33 % of women in gynecological outpatient department presented with abnormal uterine bleeding. Dilatation and curettage (D and C) have been the main diagnostic procedure in the evaluation of abnormal uterine bleeding patients for decades. The objective of the present study was to analyze the different types of endometrial histopathology of patients presented with the abnormal uterine bleeding and its correlation with the different types of abnormal uterine bleedingMethods: This is a retrospective study, conducted in a medical college in the Department of Obstetrics and Gynecology over a period of one year from June ’17 to June ’18. All cases of AUB more than 35 years of the age group who underwent D and C procedure were included in this study. Total 100 patients were analyzed.Results: Total 100 patients were analyzed. Age group ranges from 35-58 years and most common age group presenting with AUB was 35-39 years. The most common presenting complaint was menorrhagia 54% (54/100). Histopathology of endometrium showed non-organic causes in 80% (80/100) of AUB patients and the remaining 20% (20/100) had organic causes. Most common endometrial histopathology among non-organic causes was proliferative endometrium 43.75% (35/80) and the most common organic cause was endometrial polyp 40% (8/20). Endometrial hyperplasia was found in 30% (6/20) and endometrial carcinoma was found in 20% (4/20) of cases among organic causes.Conclusions: D and C is the useful and the cost-effective diagnostic procedure in the evaluation of AUB. Histopathological evaluation of endometrial samples is especially indicated in AUB patients to rule out carcinoma and preneoplastic conditions as histopathology is 100% diagnostic in cases of endometrial hyperplasia and carcinoma.

Electroencephalography recording analysis in monitoring of canine idiopathic epilepsy treated with phenobarbital. Pilot study

2014 ◽  
Vol 17 (4) ◽  
pp. 717-719 ◽  
Author(s):  
A. Bocheńska ◽  
M. Kwiatkowska ◽  
A. Pomianowski ◽  
T. Monowid ◽  
Z. Adamiak
Keyword(s):  
Veterinary Medicine ◽  
Retrospective Study ◽  
Pilot Study ◽  
Clinical Diagnosis ◽  
Diagnostic Tool ◽  
Seizure Activity ◽  
Idiopathic Epilepsy ◽  
Delta Band ◽  
Before And After ◽  
Recording Analysis

Abstract EEG recording is used in veterinary medicine as a diagnostic tool to support clinical diagnosis of idiopathic epilepsy and to identify focal seizure activity. This retrospective study was designed to compare EEG procedures in 23 dogs with idiopathic epilepsy before and after phenobarbital treatment. Differences among standard deviations for particular bands were significant. During phenobarbital treatment the delta band decreased.

Sign in / Sign up

Export Citation Format

Share Document