scholarly journals Gas Chromatography / Mass Spectroscopy Analysis, antibacterial Activity of Fixed Oil from Acacia polyacantha (Sudanese Kakamout) Seeds

2021 ◽  
pp. 21-27
Keyword(s):  
Methyl Ester ◽  
Chemical Constituents ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Antibacterial Activities ◽  
Spectroscopy Analysis ◽  
Acid Methyl ◽  
Gas Chromatography Mass Spectroscopy ◽  
Diffusion Assay ◽  
Acacia Polyacantha

The objective of this study was investigating the chemical constituents and to assess it is potential antibacterial of Fixed Oil from Acacia polyacantha (Sudanese Kakamout) Seeds. Using cold maceration method to extract the Fixed Oil from Acacia polyacantha (Sudanese Kakamout) Seeds. Where paper disc diffusion assay were employed to evaluate the antibacterial activities .GC-MS analysis were identified and quantified the constituents of Oil. Thirty seven components in the Fixed Oil from Acacia polyacantha (Sudanese Kakamout) Seeds. Five of them are major namely, the predominant compounds. were 9,12-Octadecadienoic acid (Z,Z),methyl ether(31.24%), 11-Octadecenoic acid, methyl ester(11.45%), Hexadecanoic acid, methyl ester(12.81%) , Methyl stearate(21.54%) and Eicosanoid acid, methyl ester (8.28%). The present work reveals that the extract confirm activity against Escherichia coli (18), Pseudomonas aureus (16), Staphylococcus (14) and Candida albicans (20) In conclusion, the results showed that the Oil of Acacia polyacantha is a potential source of natural antibacterial and justify its uses in folkloric medicines.

scholarly journals Phytochemical Profiling and Larval Control of Erythrina variegata Methanol Fraction against Malarial and Filarial Vector

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Mathalaimuthu Baranitharan ◽  
Barbara Sawicka ◽  
Jayapal Gokulakrishnan
Keyword(s):  
Methyl Ester ◽  
Methanol Extract ◽  
Mosquito Control ◽  
Chemical Constituents ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Methanol Fraction ◽  
Mass Spectral ◽  
Erythrina Variegata ◽  
Acid Methyl

Erythrina variegata (E. variegata) bioactive chemical has been the potential to be utilized as a good, eco-friendly approach for the control of mosquito population. In the present investigation, methanol extract using insecticidal compounds isolated against mosquito larvae kill assay was carried out. Secondary metabolism was characterized by thin layer chromatography, column chromatography, Fourier transform-infrared spectroscopy, gas chromatography-mass spectral, and identification of compound. Mosquito immature third instar larval, Anopheles stephensi, and Culex quinquefasciatus have been exposed to different concentrations of 50-250 µg/ml. Totally, larvae were death rate 98.2% (significant value 0.001b) from methanol extract and it is significant toxicity against larvae of An. stephensi and Cx. quinquefasciatus with LC50/LC99 values were 157.69/339.55 µg/ml and 137.67/297.33 µg/ml, respectively. FT-IR analysis in the functional groups such as alcohol, amines, amides, alkenes, 1⁎ amines, aromatic amines, aliphatic amines, 1⁎,2⁎ amines, and alkyl halides searched the identity of secondary metabolites, which may act as 12-Octadecenoic acid, methyl ester compound and clearly indicates being phytochemical. Chemical constituents of twenty-five compounds were identified in the methanol extract. The major components were 12-Octadecenoic acid and methyl ester (37.31%). Compound molecules consist of carbon 19 atoms (gray), hydrogen 36 atoms (greenish blue), and oxygen 2 atoms (red), indicated by the different colors. The results were obtained suggesting that, in addition to their pharmaceutical and medicine sources, 12-Octadecenoic acid, methyl ester compound can also serve as a natural mosquito control.

scholarly journals Antioxidant activity and Gas Chromatography - Mass Spectroscopy analysis of fixed oil from Peganum harmala seeds

2021 ◽  
pp. 28-34
Keyword(s):  
Methyl Ester ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Octadecenoic Acid ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Hexadecenoic Acid ◽  
Peganum Harmala ◽  
Acid Methyl

The objective of this the study was investigating the chemical constituents and to assess the potential antioxidant of Fixed Oil from Peganum harmala Seeds. Using cold maceration method to extract the Fixed Oil from Peganum harmala Seeds. The chemical constituents of Peganum harmala Oil were identified and quantified by GC-MS, where Antioxidant activities were evaluated using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity. Twenty-three components have been identified. The most abundant fatty acid methyl ester were 9,12, -Octadecadienoic acid (Z,Z) methyl ester with concentration 54.16 % followed by 9- octadecenoic acid (Z) methyl ester with concentration 21.75 %. , Hexadecenoic acid, methyl ester 9.33 %, 11-Octadecenoic acid, methyl ester 2.24 % , Methyl stearate 4.86 %and Stigmasterol 2.76 % .The DPPH assay, showed strong antioxidant potential (94, 0.01 compared with standard 91± 0.01. is a good source of antioxidants due to the presence of phenolic compounds, also is a potential source of natural antibacterial, and justify its uses in folkloric medicine.

scholarly journals Sandalwood, an Indian medicinal plant attenuates the microbial growth and influence up/down regulation of the metabolites

2021 ◽  
Author(s):  
Romana Parveen ◽  
Tooba Naz Shamsi ◽  
Sadaf Fatima
Keyword(s):  
Methyl Ester ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Trimethylsilyl Ester ◽  
E Coli ◽  
Acid Methyl ◽  
Soxhlet Apparatus ◽  
Antibacterial Assay

AbstractThe methanolic extract of sandalwood (SwME) was prepared by soxhlet apparatus and the antibacterial assay was performed. Further, the metabolite profiling of SwME and lysates of E. coli and E. coli grown in the presence of SwME was generated. SwME showed maximum inhibition against E. coli (MTCC 443) i.e. 82.71%, and minimal against B. subtilis (MTCC 736) i.e. 26.82%. The metabolome profiles of E. coli and SwME were generated using gas chromatography-mass spectrometry (GC-MS) technique. Comparative studies were done to understand to what extent metabolite modifications differ between SwME, E. coli lysate and the E. coli strain grown in presence of extract. Result revealed 23 peaks with major compounds present in E. coli were 9-Octadecenoic Acid (Z)-, Methyl Ester (26.85%), Hexadecanoic Acid, methyl ester (20.5%) and Hexadecanoic acid, trimethylsilyl ester (15.79%). When E. coli was grown in the presence of SwME, GC-MS analysis showed 25 peaks with major compounds such as 9-Octadecenoic Acid, Methyl Ester (21.97%), Hexadecanoic Acid, Methyl Ester (17.03%), and Hexadecanoic Acid, Trimethylsilyl Ester (14.96%). Correlating the metabolic profiles with the changes occurring is essential to progression their comprehension and in the development of new approaches to identify the metabolomics regulation in E. coli in response to SwME.

scholarly journals CHEMICAL CONSTITUENTS OF Bischofia javanica

2017 ◽  
Vol 55 (2) ◽  
pp. 188
Author(s):  
Nguyen Thi Mai
Keyword(s):  
Methyl Ester ◽  
Gallic Acid ◽  
Nmr Spectra ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
First Report ◽  
Acid Methyl ◽  
Bischofia Javanica ◽  
Reported Data

From the methanol extract of Bischofia javanica leaves, five compounds including 5'-b-D-glucopyranosyloxyjasmonic acid methyl ester (1), 2-(4-hydroxy-3-methoxyphenyl)ethyl-O-β-D-glucopyranoside (2), hexyl-O-β-D-glucopyranoside (3), friedelan-3-one (4), and gallic acid (5) were isolated. Their structures were elucidated by NMR spectra as well as in comparison with previous reported data. This is the first report of 1 and 2 from Bischofia javanica.

Fatty Acid Composition of Seeds of Satureja thymbra and S. cuneifolia

2003 ◽  
Vol 58 (7-8) ◽  
pp. 502-504 ◽  
Author(s):  
Ahmet C. Gören ◽  
Gökhan Bilsel ◽  
Mehmet Altun ◽  
Fatih Satıl
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Methyl Esters ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Octadecatrienoic Acid ◽  
Octadecanoic Acid ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Main Components

Abstract The chemical composition of fatty acid methyl esters (FAMEs) from seeds of S. thymbra and S. cuneifolia were analyzed by GC/MS. 7 FAMEs were identified from the seeds of S. thymbra mainly as 9-octadecenoic acid methyl ester (43.9%), hexadecanoic acid methyl ester (11.4%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (30.2%), and octadecanoic acid methyl ester (14.1%), while from the seed of S. cuneifolia 10 FAMEs were obtained with the main components, similar to S. thymbra. These were identified as 9-octadecenoic acid methyl ester (10.1%), hexadecanoic acid methyl ester (methyl palmitate, 34.6%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (6.3%) and octadecanoic acid methyl ester (1.8%).

scholarly journals Use of Fatty Acid Methyl Ester Profiles for Discrimination of Bacillus cereus T-Strain Spores Grown on Different Media

2010 ◽  
Vol 76 (6) ◽  
pp. 1902-1912 ◽  
Author(s):  
Christopher J. Ehrhardt ◽  
Vivian Chu ◽  
TeeCie Brown ◽  
Terrie L. Simmons ◽  
Brandon K. Swan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Bacillus Cereus ◽  
Fatty Acid Methyl Ester ◽  
Function Analysis ◽  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
Single Species ◽  
Sporulation Medium ◽  
Acid Methyl

ABSTRACT The goal of this study was to determine if cellular fatty acid methyl ester (FAME) profiling could be used to distinguish among spore samples from a single species (Bacillus cereus T strain) that were prepared on 10 different medium formulations. To analyze profile differences and identify FAME biomarkers diagnostic for the chemical constituents in each sporulation medium, a variety of statistical techniques were used, including nonmetric multidimensional scaling (nMDS), analysis of similarities (ANOSIM), and discriminant function analysis (DFA). The results showed that one FAME biomarker, oleic acid (18:1 ω9c), was exclusively associated with spores grown on Columbia agar supplemented with sheep blood and was indicative of blood supplements that were present in the sporulation medium. For spores grown in other formulations, multivariate comparisons across several FAME biomarkers were required to discern profile differences. Clustering patterns in nMDS plots and R values from ANOSIM revealed that dissimilarities among FAME profiles were most pronounced when spores grown with disparate sources of complex additives or protein supplements were compared (R > 0.8), although other factors also contributed to FAME differences. DFA indicated that differentiation could be maximized with a targeted subset of FAME variables, and the relative contributions of branched FAME biomarkers to group dissimilarities changed when different media were compared. When taken together, these analyses indicate that B. cereus spore samples grown in different media can be resolved with FAME profiling and that this may be a useful technique for providing intelligence about the production methods of Bacillus organisms in a forensic investigation.

Analysis of Main Flavor Substances of Low- and Full-Fat Cheese Fermented by Lactobacillus acidophilus during Ripening Process

2011 ◽  
Vol 236-238 ◽  
pp. 2395-2398
Author(s):  
Rui Xiang Zhao ◽  
Cui Qing Hu ◽  
Sheng Yang Niu ◽  
Hua Di Sun ◽  
Xiao Hong Ge
Keyword(s):  
Methyl Ester ◽  
Lactobacillus Acidophilus ◽  
Mass Fraction ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Streptococcus Thermophilus ◽  
Relative Mass ◽  
Low Fat ◽  
Acid Methyl ◽  
Low Fat Cheese

Low- and full-fat cheeses were fermented by Lactobacillus acidophilus and adjunct cultures Streptococcus thermophilus. After ripening for 90d at 4°C, the main flavor substances were determined by gas chromatograph. The results showed that more than 28 main flavor components were founded in full-fat cheese, and at least 19 were founded in low-fat cheese. In low-fat cheese, there was a higher content of 9-(Z)- octadecenoic acid and 10-hydroxy-octanoic acid methyl ester, which their relative mass fraction were 19.20% and 18.79% respectively. In full-fat cheese, there was a higher content of hexadecanoic acid methyl ester and 9-Octadecenoic acid(Z)-methyl ester, which their relative mass fraction were 24.27%and 23.37% respectively.

scholarly journals Isoprenoids and Fatty Acids Derivatives from the Chloroform Fraction of the Antimycobacterial Methanol Extract Ximeniaamericana Lam. (Olacaceae) Stem Bark

2019 ◽  
Vol 6 (2) ◽  
pp. 101-106
Author(s):  
Ozadheoghene Eriarie Afieroho ◽  
L. Lawson ◽  
Nnamdi Emenyonu
Keyword(s):  
Fatty Acids ◽  
Mycobacterium Tuberculosis ◽  
Methyl Ester ◽  
Palmitic Acid ◽  
Methanol Extract ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Stem Bark ◽  
Clinical Strains ◽  
Acid Methyl

This study investigated the triterpenoids and fatty acid derivatives, and the in vitro growth inhibitory effect against clinical strains of Mycobacteria tuberculosis of the stem bark of Ximenia Americanaa plant widely used in ethno-medicine for the treatment of bacterial and skin infections, poison, post-partum hemorrhage, anaemia, and dysentery. The macerated methanol extract (XAM) of the stem bark was evaluated for anti-tuberculosis activity using the Lowensten Jensen method against de-contaminated clinical strains of Mycobacterium tuberculosis. The XAM was fractionated by open column chromatography on a normal phase silica gel column with a 25 % stepwise gradient of chloroform-methanol as mobile phase. The constituents of the non-polar column fractions eluted with 100% chloroform were characterized using Gas Chromatography-Mass spectroscopic (GC-MS) techniques and by comparison with reference NIST library compound. The XAM (5 mg/mL) inhibited the growth of the Mycobacterium tuberculosis. GC-MS analysis of the non-polar column fractions afforded Two lupane-type triterpenoids: Lup-20-(29)-en-3-one (15) and lupeol (16), three phytosteroids: campesterol (11), stigmasterol (12) and gamma-sitosterol (14), one fridelane-type triterpenoid: Friedelan-3-one (8), one oleanane-type triterpenoid: 12-oleanen-3-one (13), and the fatty acids: Palmitic acid methyl ester (1), Palmitic acid (2), 11-octadecenoic acid methyl ester (3), Octadecanoic acid methyl ester (4), Cis-13-Octadecenoic acid (5), 10,13-octadecadiynoic acid methyl ester (6), Docosanoic acid (7), Tetracosanoic acid (9), and Hexacosanoic acid methyl ester (10). The presence of these bioactive triterpenoids and fatty acids could offer an explanation for the ethno-medicinal uses of this plant. Further work is on-going to isolate in pure form, and characterized the bioactive constituents in the XAM with the view of discovery lead compounds for the treatment of tuberculosis and associated opportunistic bacterial infections.  

scholarly journals Chemosensory response of the Phaseolus vulgaris L. (Fabaceae) weevil (Bruchus chinensis Linnaeus, 1758 (Coleoptera: Bruchidae)) to Dioscorea sansibarensis Pax. (Dioscoreaceae) bulbil essential oil

2019 ◽  
Vol 88 (2) ◽  
pp. 183-195 ◽  
Author(s):  
Godfrey Omare Mauti ◽  
Peter F. Kasigwa ◽  
Joan J.E. Munissi ◽  
Justus M. Onguso
Keyword(s):  
Methyl Ester ◽  
Phaseolus Vulgaris ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Design Experiment ◽  
Hexadecanoic Acid ◽  
Botanical Pesticide ◽  
Acid Methyl ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract Dioscorea sansibarensis bulbils are well known for their toxicity and medicinal applications. In this study, the attraction and mortality of the Phaseolus vulgaris weevil (Bruchus chinensis) caused by Dioscorea sansibarensis bulbil oil was examined in a Completely Randomized Design experiment using a Y-tube olfactometer on a laboratory bench. Statistical analysis at P < 0.05 showed 10 µL had an attraction of 42.67%. In the case of mortality, the results showed that Phaseolus vulgaris seeds treated with 40 µL of the bulbil oil had a harmonic mean of 55.8% mortality of the weevil population, which was higher than with 10 µL of Actellic 50 EC. GC-MS analysis identified 17 compounds in the bulbil oil, the main constituents of which were 2- pentanone, 4-hydroxy-4-methyl- (17.8%), phenol, 4-ethyl- (11.41%), hexadecanoic acid, methyl ester (11.05%), phenol (7.35%), 9-octadecenoic acid, (E)- (7.2%) and phenol, 4-ethyl-2-methoxy- (7.14%). Attraction by bulbil oil may have been due to the presence of phenolic compounds, and mortality may have been due to hexadecanoic acid, methyl ester and 9-octadecenoic acid, (E)-. Attractants are used for the surveillance and detection of an infestation in stored products. The added advantage of the bulbil oil to cause mortality makes Dioscorea sansibarensis bulbil oil useful in the formulation of a botanical pesticide in stored pest management.

scholarly journals BIOCHEMICAL PROFILE OF Calotropis procera FLOWERS

2021 ◽  
Vol 27 (3) ◽  
pp. 341-349
Author(s):  
Malik Fiaz Hussain Ferdosi ◽  
Iqra Haider Khan ◽  
Arshad Javaid ◽  
Ayesha Munir
Keyword(s):  
Methyl Ester ◽  
Octadecadienoic Acid ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Calotropis Procera ◽  
Biochemical Profile ◽  
Hexadecanoic Acid ◽  
Flower Extract ◽  
Acid Methyl ◽  
Peak Areas

Calotropis procera is a medicinal weed of family Asclepiadaceae. This study was carried out to explore the biochemical profile of C. procera flowers collected from Southern Punjab region of Pakistan. Methanolic flower extract of C. procera was subjected to GC-MS analysis. There were 30 compounds identified in this extract. The predominant compound was γ-sitosterol with 15.39% peak area. Other abundantly occurring compounds included stigmasterol (9.22%), 9,12-octadecadienoic acid (Z,Z)-, methyl ester (9.01%), campesterol (8.63%), α-amyrin acetate (8.25%), β-amyrin (8.09%), hexadecanoic acid, methyl ester (7.91%), 11-octadecenoic acid, methyl ester (6.15%), and 2-methoxy-4-vinylphenol (5.66%). Moderately abundant compounds included nonacos-1-ene (2.83%), methyl stearate (1.57%), pentacosane (1.44%), phytol (1.33%), heptacos-1-ene (1.20%), heneicosane (1.19%), and 1-hexacosene (1.09%). The remaining less abundant compounds were present with peak areas less than 1%. Literature survey showed that the major compounds identified in the flower extract of C. procera possess various bioactivities including antidiabetic, anticancer, antihyperglycemic, antioxidant, antimicrobial and anti-inflamatory.

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