scholarly journals Prevalence of rumen impaction with non-biodegradable materials in cattle in Ibadan, Nigeria

2021 ◽  
Vol 39 (1) ◽  
pp. 211-217
Author(s):  
J. F. Akinrinmade ◽  
S. A. Akinrinde
Keyword(s):  
Risk Factors ◽  
Health Status ◽  
Serum Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Biodegradable Materials ◽  
Blood Samples ◽  
Blood Indices ◽  
Phosphorus Levels ◽  
Ibadan Nigeria

A study was conducted on 3031 cattle slaughtered in Ibadan over a period of three months to determine the prevalence of rumen impaction with indigestible garbage and evaluate breed, sex, age and some blood indices as risk factors. Thorough antemortem and postmortern examinations were carried out on each of the animals to determine the breed, health status, sex, age and presence of indigestible garbage in the rumen while blood samples were collected and analysed only for animals with indigestible garbage, to determine total serum protein and phosphorus levels. Results showed overall prevalence of rumen impaction was 10.77% and it differed significantly between breeds, but was highest in the Red Bororo (RB). Sex and age had significant influence on the prevalence of rumen impaction. It was significantly higher (P < 0.05) in females than in males and in older animals than in younger ones. Total serum protein and phosphorus levels were significantly lower than reference values in animals with rumen impaction.   

scholarly journals Refractometry as an alternative to the biuret method for measuring total serum proteins in Podocnemis expansa (Podocnemididae) and Phrynops geoffroanus (Chelidae)

2017 ◽  
Vol 47 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Lourdes Marina Bezerra PESSOA ◽  
Maíra Gonçalves da Mota LIMA ◽  
Filipe Tavares CARNEIRO ◽  
Nathalia Salgado ZANANI ◽  
Marcela Corrêa SCALON ◽  
...  
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Health Condition ◽  
Total Serum Protein ◽  
Total Serum ◽  
Blood Samples ◽  
Podocnemis Expansa ◽  
Biuret Method ◽  
Mean Concentration

ABSTRACT Total serum protein is a significant indicator of health condition in animals. The aim of this study was to analyze the precision of the portable refractometer in determining the concentration of total serum proteins in Podocnemis expansa and Phrynops geoffroanus. A total of 26 animals were used. The blood samples were collected from the supraoccipital sinus and stored in tubes without anticoagulant. Total serum protein was determined using both the biuret reaction and refractometry. The total serum protein mean concentration (g dL-1) with biuret method and refractometry for P. expansa were 3.16 and 3.2; and for P. geoffroanus were 3.56 and 2.72, respectively. These results indicate that total serum protein values can be determined with precision in P. expansa and P. geoffroanus using a portable refractometer.

PARALLEL STUDIES OF COMPLEMENT AND COAGULATION IV. EFFECT OF CARBON TETRACHLORIDE

1951 ◽  
Vol 29 (2) ◽  
pp. 48-58
Author(s):  
Christine E. Rice ◽  
Paul Boulanger ◽  
P. J. G. Plummer
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Serum Protein ◽  
Guinea Pigs ◽  
Fatty Degeneration ◽  
Blood Groups ◽  
Total Serum Protein ◽  
Total Serum ◽  
Coagulation Time ◽  
Complement Activity

To determine whether liver injury would result in a parallel decline in the complement titer and coagulative properties of the blood, groups of guinea pigs were given series of injections of the liver poison, carbon tetrachloride. Marked fatty degeneration of the liver, a decline in total serum protein and albumin, a decrease in complement activity, and a prolongation of coagulation time was observed in the treated animals. A general relationship was noted between the albumin-globulin ratio and the complement titer of the serum and between the complement titer and the coagulation time of the plasma.

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

THE EFFECT OF REPEATED HEAT AND COLD EXPOSURE ON SERUM PROTEIN COMPOSITION IN MAN

1967 ◽  
Vol 45 (4) ◽  
pp. 571-575 ◽  
Author(s):  
W. F. Blatt ◽  
J. Kerkay
Keyword(s):  
Serum Protein ◽  
Cold Exposure ◽  
Total Protein ◽  
Protein Composition ◽  
Total Serum Protein ◽  
Total Serum ◽  
Heat Acclimatization ◽  
Albumin Content ◽  
Protein Serum ◽  
Lipoprotein Distribution

Total protein, serum protein, and lipoprotein electrophoretic distribution and hematocrit values were determined in two groups of men during acclimatization to 6 weeks of cold and 11 days of heat respectively. After 3 weeks of cold exposure total serum protein and albumin content decreased, while the globulin fractions increased; thus, the calculated albumin/globulin ratio was significantly depressed. During the last 2 weeks, these parameters gradually returned to control values. Overall, the protein changes during heat acclimatization were minimal, although the globulins decreased slightly, yielding a small increase in the albumin/globulin ratio. The hematocrit levels were significantly lowered during both environmental exposures, whereas the lipoprotein distribution remained essentially unchanged.

scholarly journals Serum proteinogram of the Campeiro horse

2019 ◽  
Vol 71 (2) ◽  
pp. 363-368
Author(s):  
A.F. Souza ◽  
J. Schade ◽  
A.F. Ramos ◽  
M.S.M. Albuquerque ◽  
G.V. Fonteque ◽  
...  
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Reference Interval ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Concentrations ◽  
Reproductive Condition ◽  
Serum Fractions ◽  
Pregnancy Status ◽  
Biuret Method

ABSTRACT The aim of this study to measure the fractions of the total serum proteins of the Campeiro horse and identify the influences of biological variants. Blood samples were taken in 138 horses of the breed Campeiro for measuring the concentration of total serum protein by the biuret method. Serum concentrations of protein fractions were measured by electrophoresis using agarose gel. Groups were formed according to age, sex and reproductive condition. The average values of serum fractions: albumin (2.85±0.36g/dl), alpha 1 (0.28±0.11g/dl), alpha 2 (0.26±0.08g/dL) beta 1 (0.57±0.15g/dl), beta 2 (0.89±0.28g/dL), gamaglobulinas (1.86±0.34g/dL), albumin/globulin ratio (0.75±0.18) and 2.5% percentile and 97.5% had slight differences in relation to the reference interval proposed for the species. They observed higher values of alpha 1 and 2 globulins in the group from that had six to eight years old and gammaglobulins in group above 13 years old. Serum protein concentrations were similar in horses and mares and between non-pregnant and pregnant. Sex and pregnancy status did not affect serum proteinogram. Alpha and gammaglobulins have higher values as the age increases. Serum proteinogram of Campeiro horses shows variations that have to be considered in the interpretation of laboratory tests.

Automated Procedure for Simultaneously Measuring Total Globulin and Total Protein in Serum

1971 ◽  
Vol 17 (4) ◽  
pp. 301-306 ◽  
Author(s):  
John Savory ◽  
M Geraldine Heintges ◽  
Robert E Sobel
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Continuous Flow ◽  
Gamma Globulin ◽  
Glyoxylic Acid ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Globulin ◽  
Automated Method ◽  
A Minor

Abstract An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acetyltryptophan to human globulin has been derived. Values for total serum globulin obtained by this new automated procedure correlate closely with values obtained by electrophoresis, but do not agree with values obtained by use of procedures involving binding of anionic dye. Recovery of gamma globulin added to serum is essentially quantitative; the day-to-day precision (CV) is 4.31%.

Precipitation of serum proteins by the lectin from wheat-germ.

1987 ◽  
Vol 33 (1) ◽  
pp. 185-186 ◽  
Author(s):  
W E Schreiber ◽  
L Whitta
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Protein ◽  
Wheat Germ ◽  
Serum Proteins ◽  
Total Serum Protein ◽  
Total Serum ◽  
Wheat Germ Lectin

Abstract We investigated the composition of the precipitate that forms when wheat-germ lectin derived from Triticum vulgaris is added to serum. A number of serum proteins are precipitated, representing about 2.5% of the total serum protein. This study demonstrates that the interaction of this lectin with the bone isoenzyme of alkaline phosphatase is not specific.

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