scholarly journals An in vitro assessment of the responses of human dermal fibroblast seeded on 3D printed thermoplastic polyurethane scaffold

2022 ◽  
Author(s):  
Ufkay KARABAY ◽  
Selma AYDEMİR ◽  
Mehtap YUKSEL EGRILMEZ ◽  
Başak BAYKARA ◽  
R. Bugra HUSEMOGLU
Keyword(s):  
Dermal Fibroblast ◽  
Thermoplastic Polyurethane ◽  
Human Dermal Fibroblast ◽  
Polyurethane Scaffold ◽  
3D Printed ◽  
In Vitro Assessment

A novel design of wound bandage using heparin-polyvinylpyrrolidone/TiO2 nanocomposite to improved antibacterial treatment and burn wound healing effect: In vitro and in vivo evaluation

2021 ◽  
Vol 11 (11) ◽  
pp. 1808-1818
Author(s):  
Xiuli Li ◽  
Jigang Wang ◽  
Xin Li ◽  
Xiaoqian Hou ◽  
Hao Wang ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Dermal Fibroblast ◽  
Synergistic Effects ◽  
Bacterial Species ◽  
Human Dermal Fibroblast ◽  
Burn Wound ◽  
In Vivo Evaluation ◽  
Closure Rate

In our current study, porous heparin-polyvinylpyrrolidone/TiO2 nanocomposite (HpPVP/TiO2) bandage were prepared via the incorporation of TiO2 into HpPVP hydrogels for biomedical applications such as burn infection. The effect of the HpPVP hydrogels and the nanoparticles of TiO2 composition on the functional group and the surface properties of the as-fabricated bandages were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometry (XRD). The presence of TiO2 nanoparticles created the internal structure of the HpPVP hydrogel that aids in a homogeneous porous structure, as indicated by the scanning electron microscope (SEM). The size distribution of the TiO2 nanoparticles was measured using a transmission electron microscope (TEM). The studies on the mechanical properties of the HpPVP hydrogel indicate that the addition of TiO2 nanoparticles increases its strength. The prepared HpPVP/TiO2 nanocomposite dressing has excellent antimicrobial activity were tested against bacterial species (Staphylococcus aureus and Escherichia coli) and has good biocompatibility against human dermal fibroblast cells (HFFF2) for biological applications. In addition, in vivo evaluations in Kunming mice exposed that the as-fabricated HpPVP/TiO2 nanocomposite bandages increased the wound curing and facilitated accelerate skin cell construction along with collagen development. The synergistic effects of the HpPVP/TiO2 nanocomposite hydrogel dressing material, such as its excellent hydrophilic nature, good bactericidal activity, biocompatibility and wound closure rate through in vivo test makes it a suitable candidate for burn infections.

scholarly journals The Effect of Parietin Isolated From Rheum ribes L on In Vitro Wound Model Using Human Dermal Fibroblast Cells

2019 ◽  
Vol 18 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Gulsah Gundogdu ◽  
Koksal Gundogdu ◽  
Kemal Alp Nalci ◽  
Alper Kursat Demirkaya ◽  
Seymanur Yılmaz Tascı ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Viability ◽  
Dermal Fibroblast ◽  
Dose Range ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cells ◽  
Wound Model ◽  
Dpph Method ◽  
Pipette Tip

Parietin is one of the well-known anthraquinone compounds that can be extracted from Rheum ribes L. In this study, we aimed to investigate the effects of parietin isolated from Rheum ribes L on an in vitro wound model using human dermal fibroblast cells and compare its effectiveness against zinc. The antioxidant effect of parietin was determined by using the 1,1-diphenyl-2-picrylhydrazine (DPPH) method. Human dermal fibroblast cells were cultured in proculture medium and were kept until 100% confluence was achieved. The wound model was created by using a pipette tip. After that, different concentrations of parietin and zinc (final concentrations in the well to be 5-250 µM and 25-200 µM, respectively) were added into the medium. The proliferation-inducing effect on cell viability was determined by using MTT assay. Images of cells were taken at 0, 12, and 24 hours. According to the DPPH method, parietin exhibited have antioxidant activity. According to the MTT results, parietin exhibited significant proliferation-inducing effect on cell viability in a dose range of 5 to 10 M, and zinc showed significant proliferation-inducing effect on cell viability at dose 50 µM ( P < .05). In addition, the image of cell proliferation was also shown at the same doses at 24 hours. In this study, we claim that parietin induces cell proliferation at low doses in cases of dermal fibroblast loss. In conclusion, parietin as an alternative to zinc in wound healing could be used by clinicians in the future with more extensive studies.

scholarly journals In VitroWound Healing Potential and Identification of Bioactive Compounds fromMoringa oleiferaLam

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Abubakar Amali Muhammad ◽  
Nur Aimi Syarina Pauzi ◽  
Palanisamy Arulselvan ◽  
Faridah Abas ◽  
Sharida Fakurazi
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Dermal Fibroblast ◽  
Bioactive Compound ◽  
Scratch Test ◽  
Human Dermal Fibroblast ◽  
Aqueous Fraction ◽  
Bioactive Fraction ◽  
Hdf Cells

Moringa oleiferaLam. (M. oleifera) from the monogeneric familyMoringaceaeis found in tropical and subtropical countries. The present study was aimed at exploring thein vitrowound healing potential ofM. oleiferaand identification of active compounds that may be responsible for its wound healing action. The study included cell viability, proliferation, and wound scratch test assays. Different solvent crude extracts were screened, and the most active crude extract was further subjected to differential bioguided fractionation. Fractions were also screened and most active aqueous fraction was finally obtained for further investigation. HPLC and LC-MS/MS analysis were used for identification and confirmation of bioactive compounds. The results of our study demonstrated that aqueous fraction ofM. oleiferasignificantly enhanced proliferation and viability as well as migration of human dermal fibroblast (HDF) cells compared to the untreated control and other fractions. The HPLC and LC-MS/MS studies revealed kaempferol and quercetin compounds in the crude methanolic extract and a major bioactive compound Vicenin-2 was identified in the bioactive aqueous fraction which was confirmed with standard Vicenin-2 using HPLC and UV spectroscopic methods. These findings suggest that bioactive fraction ofM. oleiferacontaining Vicenin-2 compound may enhance faster wound healingin vitro.

scholarly journals In Vitro Bioeffects of Polyelectrolyte Multilayer Microcapsules Post-Loaded with Water-Soluble Cationic Photosensitizer

Pharmaceutics ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 610 ◽  
Author(s):  
Alexey V. Ermakov ◽  
Roman A. Verkhovskii ◽  
Irina V. Babushkina ◽  
Daria B. Trushina ◽  
Olga A. Inozemtseva ◽  
...  
Keyword(s):  
Targeted Delivery ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Water Soluble ◽  
Zinc Phthalocyanine ◽  
Bacterial Strains ◽  
Normal Human Dermal Fibroblast ◽  
Drug Dosing ◽  
Normal Human

Microencapsulation and targeted delivery of cytotoxic and antibacterial agents of photodynamic therapy (PDT) improve the treatment outcomes for infectious diseases and cancer. In many cases, the loss of activity, poor encapsulation efficiency, and inadequate drug dosing hamper the success of this strategy. Therefore, the development of novel and reliable microencapsulated drug formulations granting high efficacy is of paramount importance. Here we report the in vitro delivery of a water-soluble cationic PDT drug, zinc phthalocyanine choline derivative (Cholosens), by biodegradable microcapsules assembled from dextran sulfate (DS) and poly-l-arginine (PArg). A photosensitizer was loaded in pre-formed [DS/PArg]4 hollow microcapsules with or without exposure to heat. Loading efficacy and drug release were quantitatively studied depending on the capsule concentration to emphasize the interactions between the DS/PArg multilayer network and Cholosens. The loading data were used to determine the dosage for heated and intact capsules to measure their PDT activity in vitro. The capsules were tested using human cervical adenocarcinoma (HeLa) and normal human dermal fibroblast (NHDF) cell lines, and two bacterial strains, Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. Our results provide compelling evidence that encapsulated forms of Cholosens are efficient as PDT drugs for both eukaryotic cells and bacteria at specified capsule-to-cell ratios.

scholarly journals Anti-Melanogenesis, Antioxidant and Anti-Tyrosinase Activities of Scabiosa columbaria L.

Processes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 236
Author(s):  
Wilfred Otang-Mbeng ◽  
Idowu Jonas Sagbo
Keyword(s):  
Plant Extract ◽  
Dermal Fibroblast ◽  
Reducing Power ◽  
Human Dermal Fibroblast ◽  
Collagen Content ◽  
Dependent Manner ◽  
Tyrosinase Enzyme ◽  
Fluorescence Imaging Microscopy ◽  
Dose Dependent

Scabiosa columbaria is a plant traditionally used to treat skin ailments, such as scabies, wound bruises, sores and hyperpigmentation. To find a novel skin depigmenting agent, the present study was investigated to determine the possible anti-melanogenesis, antioxidant and anti-tyrosinase effects of methanol extract of S. columbaria leaves. Cytotoxicity towards human dermal fibroblast (MRHF) cells was assessed using the live-cell fluorescence imaging microscopy. The inhibitory effects of the extract on tyrosinase, collagenase and melanin synthesis were also investigated using standard in vitro method, while ferric reducing power (FRAP) was used to determine the antioxidant potential of the plant extract. The effect of the extract on collagen content in MRHF cells was also investigated. The plant extract displayed no meaningful cytotoxicity towards MRHF cells and no significant cell death was recorded at all the tested concentrations. The extract (25–100 µg/mL) effectively decreased melanin content in B16F10 (mouse melanoma) cells with moderate inhibition of tyrosinase enzyme in a dose-dependent manner. However, the extract also demonstrated no significant effect on collagenase and collagen content in MRHF cells, but showed strong antioxidant activity at the concentrations tested. The results suggest that S. columbaria could be a promising candidate in the treatment of skin hyperpigmentation disorders

scholarly journals Resveratrol-Loaded Levan Nanoparticles Produced by Electrohydrodynamic Atomization Technique

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2582
Author(s):  
Ezgi Cinan ◽  
Sumeyye Cesur ◽  
Merve Erginer Haskoylu ◽  
Oguzhan Gunduz ◽  
Ebru Toksoy Oner
Keyword(s):  
Dermal Fibroblast ◽  
Halophilic Bacteria ◽  
Particle Diameter ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Delivery Systems ◽  
Cellular Interaction ◽  
In Vitro Biocompatibility ◽  
Electrohydrodynamic Atomization

Considering the significant advances in nanostructured systems in various biomedical applications and the escalating need for levan-based nanoparticles as delivery systems, this study aimed to fabricate levan nanoparticles by the electrohydrodynamic atomization (EHDA) technique. The hydrolyzed derivative of levan polysaccharide from Halomonas smyrnensis halophilic bacteria, hydrolyzed Halomonas levan (hHL), was used. Nanoparticles were obtained by optimizing the EHDA parameters and then they were characterized in terms of morphology, molecular interactions, drug release and cell culture studies. The optimized hHL and resveratrol (RS)-loaded hHL nanoparticles were monodisperse and had smooth surfaces. The particle diameter size of hHL nanoparticles was 82.06 ± 15.33 nm. Additionally, release of RS from the fabricated hHL nanoparticles at different pH conditions were found to follow the first-order release model and hHL with higher RS loading showed a more gradual release. In vitro biocompatibility assay with human dermal fibroblast cell lines was performed and cell behavior on coated surfaces was observed. Nanoparticles were found to be safe for healthy cells. Consequently, the fabricated hHL-based nanoparticle system may have potential use in drug delivery systems for wound healing and tissue engineering applications and surfaces could be coated with these electrosprayed particles to improve cellular interaction.

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