scholarly journals In VitroWound Healing Potential and Identification of Bioactive Compounds fromMoringa oleiferaLam

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Abubakar Amali Muhammad ◽  
Nur Aimi Syarina Pauzi ◽  
Palanisamy Arulselvan ◽  
Faridah Abas ◽  
Sharida Fakurazi
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Dermal Fibroblast ◽  
Bioactive Compound ◽  
Scratch Test ◽  
Human Dermal Fibroblast ◽  
Aqueous Fraction ◽  
Bioactive Fraction ◽  
Hdf Cells

Moringa oleiferaLam. (M. oleifera) from the monogeneric familyMoringaceaeis found in tropical and subtropical countries. The present study was aimed at exploring thein vitrowound healing potential ofM. oleiferaand identification of active compounds that may be responsible for its wound healing action. The study included cell viability, proliferation, and wound scratch test assays. Different solvent crude extracts were screened, and the most active crude extract was further subjected to differential bioguided fractionation. Fractions were also screened and most active aqueous fraction was finally obtained for further investigation. HPLC and LC-MS/MS analysis were used for identification and confirmation of bioactive compounds. The results of our study demonstrated that aqueous fraction ofM. oleiferasignificantly enhanced proliferation and viability as well as migration of human dermal fibroblast (HDF) cells compared to the untreated control and other fractions. The HPLC and LC-MS/MS studies revealed kaempferol and quercetin compounds in the crude methanolic extract and a major bioactive compound Vicenin-2 was identified in the bioactive aqueous fraction which was confirmed with standard Vicenin-2 using HPLC and UV spectroscopic methods. These findings suggest that bioactive fraction ofM. oleiferacontaining Vicenin-2 compound may enhance faster wound healingin vitro.

Effects Of (1→3), (1→6)-β-D-Glucan Behavior in Human Dermal Fibroblast Cells under Serum Starvation

2007 ◽  
Vol 342-343 ◽  
pp. 401-404 ◽  
Author(s):  
Yeon I Woo ◽  
Hyun Joo Son ◽  
Hye Ryeon Lim ◽  
Mi Hee Lee ◽  
Hyun Sook Baek ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Positive Influence ◽  
Dermal Fibroblasts ◽  
Serum Starvation ◽  
Healing Agent ◽  
And Migration ◽  
Free Serum

Glucans have been reported to stimulate immunity and to promote wound healing. Adult human dermal fibroblast (aHDF) cultured in serum free (serum-starvation). Proliferation of aHDF was measured at various concentrations of β-glucan by MTT assay, and migration was observed for 36h on microscope. The result of fibroblast bioassay, β-glucan had positive influence. In this study, the direct effects of β-glucan on proliferation and migration of human dermal fibroblasts were examined in vitro. That means β-D-glucan has the effect to enhance proliferation and aHDF migration speed, and has the potential as a wound healing agent.

The Evaluation of the Effects of Nanoemulsion Formulations Containing Boron and/or Zinc on the Wound Healing in Diabetic Rats

2020 ◽  
pp. 153473462096189
Author(s):  
Gulsah Gundogdu ◽  
Kemal Alp Nalci ◽  
Afife Busra Ugur Kaplan ◽  
Koksal Gundogdu ◽  
Tuba Demirci ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblast ◽  
Clinical Problem ◽  
Human Dermal Fibroblast ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Scratch Assay ◽  
Positive Effects ◽  
Droplet Sizes ◽  
Hdf Cells

Wound healing remains a challenging clinical problem, especially in the presence of diabetes. Diabetic patients have the impaired ability to fight infection and insufficient inflammatory response. The aim of this study was to evaluate the effects of boronophenylalanine (BFA) and/or Zn-containing nanoemulsion (NE) formulations on wound healing in diabetic rats. MTT and scratch assays were performed to evaluate the proliferative effects of BFA and/or Zn on human dermal fibroblast (HDF) cells and the migration of these cells, respectively. The BFA and/or Zn-NE were prepared, and the effects of NEs on wound healing in diabetic rats were evaluated by applying once a day for 14 days. MTT assay showed that 10 to 25 µM BFA and/or 50 µM Zn had very significant positive effects on cell proliferation. In the scratch assay, 10 µM BFA significantly increased the migration of HDF cell compared with control. The droplet sizes of all the NEs were <115 nm and their zeta potential values were in range of (−) 23.9 ± 2.356 to (−) 33.1 ± 1.438 mV. There was a significant reduction in the wound contraction values (%) of the groups treated with the BFA and/or Zn-NE on the 14th day compared with the untreated diabetic rats group. According to histopathological findings, wound healing was nearly complete in BFA and/or Zn-NE compared with untreated diabetic rats. Especially, the group treated with the NE containing the low concentration of BFA showed highly promising results in wound healing of diabetic rats within 14 days with complete epithelialization and the completely closed wound area.

scholarly journals Concentration Dependent Effect of Human Dermal Fibroblast Conditioned Medium (DFCM) from Three Various Origins on Keratinocytes Wound Healing

2020 ◽  
Vol 21 (8) ◽  
pp. 2929
Author(s):  
Manira Maarof ◽  
Shiplu Roy Chowdhury ◽  
Aminuddin Saim ◽  
Ruszymah Bt Hj Idrus ◽  
Yogeswaran Lokanathan
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Control Group ◽  
Serum Free ◽  
Specific Medium ◽  
Polymerase Chain ◽  
And Migration

Fibroblasts secrete many essential factors that can be collected from fibroblast culture medium, which is termed dermal fibroblast conditioned medium (DFCM). Fibroblasts isolated from human skin samples were cultured in vitro using the serum-free keratinocyte-specific medium (Epilife (KM1), or define keratinocytes serum-free medium, DKSFM (KM2) and serum-free fibroblast-specific medium (FM) to collect DFCM-KM1, DFCM-KM2, and DFCM-FM, respectively). We characterised and evaluated the effects of 100–1600 µg/mL DFCM on keratinocytes based on attachment, proliferation, migration and gene expression. Supplementation with 200–400 µg/mL keratinocyte-specific DFCM-KM1 and DFCM-KM2 enhanced the attachment, proliferation and migration of sub-confluent keratinocytes, whereas 200–1600 µg/mL DFCM-FM significantly increased the healing rate in the wound healing assay, and 400–800 µg/mL DFCM-FM was suitable to enhance keratinocyte attachment and proliferation. A real-time (RT2) profiler polymerase chain reaction (PCR) array showed that 42 genes in the DFCM groups had similar fold regulation compared to the control group and most of the genes were directly involved in wound healing. In conclusion, in vitro keratinocyte re-epithelialisation is supported by the fibroblast-secreted proteins in 200–400 µg/mL DFCM-KM1 and DFCM-KM2, and 400–800 µg/mL DFCM-FM, which could be useful for treating skin injuries.

scholarly journals Efficacy ofAnnona squamosaL in the Synthesis of Glycosaminoglycans and Collagen during Wound Repair in Streptozotocin Induced Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Thangavel Ponrasu ◽  
Lonchin Suguna
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Cellular Proliferation ◽  
Dermal Fibroblast ◽  
Diabetic Control ◽  
Human Dermal Fibroblast ◽  
Diabetic Rats ◽  
Histological Evaluation ◽  
Annona Squamosa

The aim of this work was to find out the effects ofAnnona squamosaon the formation of glycosaminoglycans and collagen during wound healing in normal and diabetic rats. Diabetes induced rats were segregated into 4 groups, each containing six animals. Groups I and III served as the normal and diabetic control while groups II and IV served as normal and diabetic treated. The animals were treated with 200 μL ofAnnona squamosaextract topically. The granulation tissues formed were removed on the 8th day and the amount of glycosaminoglycans (GAGs) and collagen formed was evaluated by sequential extraction and SDSPAGE, respectively. Histological evaluation was also carried out using Masson's trichrome stain.In vitrowound healing efficacy ofA. squamosain human dermal fibroblast culture (HDF) was also carried out. The fibroblasts treated with varying concentrations ofA. squamosawere examined for proliferation and closure of the wound area and photographed.A. squamosaincreased cellular proliferation in HDF culture. The granulation tissues of treated wounds showed increased levels of glycosaminoglycans(P<0.05)and collagen which were also confirmed by histopathology. The results strongly substantiate the beneficial effects ofA. squamosaon the formation of glycosaminoglycans and collagen during wound healing.

A novel design of wound bandage using heparin-polyvinylpyrrolidone/TiO2 nanocomposite to improved antibacterial treatment and burn wound healing effect: In vitro and in vivo evaluation

2021 ◽  
Vol 11 (11) ◽  
pp. 1808-1818
Author(s):  
Xiuli Li ◽  
Jigang Wang ◽  
Xin Li ◽  
Xiaoqian Hou ◽  
Hao Wang ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Dermal Fibroblast ◽  
Synergistic Effects ◽  
Bacterial Species ◽  
Human Dermal Fibroblast ◽  
Burn Wound ◽  
In Vivo Evaluation ◽  
Closure Rate

In our current study, porous heparin-polyvinylpyrrolidone/TiO2 nanocomposite (HpPVP/TiO2) bandage were prepared via the incorporation of TiO2 into HpPVP hydrogels for biomedical applications such as burn infection. The effect of the HpPVP hydrogels and the nanoparticles of TiO2 composition on the functional group and the surface properties of the as-fabricated bandages were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometry (XRD). The presence of TiO2 nanoparticles created the internal structure of the HpPVP hydrogel that aids in a homogeneous porous structure, as indicated by the scanning electron microscope (SEM). The size distribution of the TiO2 nanoparticles was measured using a transmission electron microscope (TEM). The studies on the mechanical properties of the HpPVP hydrogel indicate that the addition of TiO2 nanoparticles increases its strength. The prepared HpPVP/TiO2 nanocomposite dressing has excellent antimicrobial activity were tested against bacterial species (Staphylococcus aureus and Escherichia coli) and has good biocompatibility against human dermal fibroblast cells (HFFF2) for biological applications. In addition, in vivo evaluations in Kunming mice exposed that the as-fabricated HpPVP/TiO2 nanocomposite bandages increased the wound curing and facilitated accelerate skin cell construction along with collagen development. The synergistic effects of the HpPVP/TiO2 nanocomposite hydrogel dressing material, such as its excellent hydrophilic nature, good bactericidal activity, biocompatibility and wound closure rate through in vivo test makes it a suitable candidate for burn infections.

scholarly journals Yerba Mate Extract in Microfibrillated Cellulose and Corn Starch Films as a Potential Wound Healing Bandage

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2807
Author(s):  
Meysam Aliabadi ◽  
Bor Shin Chee ◽  
Mailson Matos ◽  
Yvonne J. Cortese ◽  
Michael J. D. Nugent ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Corn Starch ◽  
Microfibrillated Cellulose ◽  
Ilex Paraguariensis ◽  
Mechanical And Thermal Properties ◽  
Yerba Mate ◽  
Cellulose Films ◽  
Ultrafine Grinding

Microfibrillated cellulose films have been gathering considerable attention due to their high mechanical properties and cheap cost. Additionally, it is possible to include compounds within the fibrillated structure in order to confer desirable properties. Ilex paraguariensis A. St.-Hil, yerba mate leaf extract has been reported to possess a high quantity of caffeoylquinic acids that may be beneficial for other applications instead of its conventional use as a hot beverage. Therefore, we investigate the effect of blending yerba mate extract during and after defibrillation of Eucalyptus sp. bleached kraft paper by ultrafine grinding. Blending the extract during defibrillation increased the mechanical and thermal properties, besides being able to use the whole extract. Afterwards, this material was also investigated with high content loadings of starch and glycerine. The results present that yerba mate extract increases film resistance, and the defibrillated cellulose is able to protect the bioactive compounds from the extract. Additionally, the films present antibacterial activity against two known pathogens S. aureus and E. coli, with high antioxidant activity and increased cell proliferation. This was attributed to the bioactive compounds that presented faster in vitro wound healing, suggesting that microfibrillated cellulose (MFC) films containing extract of yerba mate can be a potential alternative as wound healing bandages.

scholarly journals Protocatechuic Aldehyde Attenuates UVA-induced Photoaging in Human Dermal Fibroblast Cells by Suppressing MAPKs/AP-1 and NF-κB Signaling Pathways

2020 ◽  
Vol 21 (13) ◽  
pp. 4619
Author(s):  
Yuling Ding ◽  
Chanipa Jiratchayamaethasakul ◽  
Seung-Hong Lee
Keyword(s):  
Nitric Oxide ◽  
Protective Effect ◽  
Signaling Pathways ◽  
Inflammatory Responses ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Potential Candidate ◽  
Ros Scavenging ◽  
Protocatechuic Aldehyde ◽  
Hdf Cells

Ultraviolet radiation (UV) is a major causative factor of DNA damage, inflammatory responses, reactive oxygen species (ROS) generation and a turnover of various cutaneous lesions resulting in skin photoaging. The purpose of this study is to investigate the protective effect of protocatechuic aldehyde (PA), which is a nature-derived compound, against UVA-induced photoaging by using human dermal fibroblast (HDF) cells. In this study, our results indicated that PA significantly reduced the levels of intracellular ROS, nitric oxide (NO), and prostaglandins-E2 (PGE2) in UVA-irradiated HDF cells. It also inhibited the levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression. Besides, PA significantly suppressed the expression of matrix metalloproteinases-1 (MMP-1) and pro-inflammatory cytokines and promoted collagen synthesis in the UVA-irradiated HDF cells. These events occurred through the regulation of activator protein 1 (AP-1), nuclear factor-κB (NF-κB), and p38 signaling pathways in UVA-irradiated HDF cells. Our findings suggest that PA enhances the protective effect of UVA-irradiated photoaging, which is associated with ROS scavenging, anti-wrinkle, and anti-inflammatory activities. Therefore, PA can be a potential candidate for the provision of a protective effect against UVA-stimulated photoaging in the pharmaceutical and cosmeceutical industries.

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