scholarly journals Variation in <i>KRTAP6-1</i> affects wool fibre diameter in New Zealand Romney ewes

2019 ◽  
Vol 62 (2) ◽  
pp. 509-515 ◽  
Author(s):  
Wenhao Li ◽  
Hua Gong ◽  
Huitong Zhou ◽  
Jiqing Wang ◽  
Shaobin Li ◽  
...  
Keyword(s):  
New Zealand ◽  
Fibre Diameter ◽  
Wool Fibre ◽  
Chain Reaction ◽  
Genotype Effect ◽  
Single Stranded Conformational Polymorphism ◽  
Tan Sheep ◽  
Polymerase Chain ◽  
Fibre Traits ◽  
Breed Sheep

Abstract. Variation in KRTAP6-1 has been reported to affect wool fibre traits in Merino cross-breed sheep and Chinese Tan sheep, but little is known about whether these effects persist in other breeds. In this study, variation in KRTAP6-1 was investigated in 290 New Zealand (NZ) Romney ewes sired by 16 different rams. Polymerase chain reaction single-stranded conformational polymorphism (PCR-SSCP) analysis revealed four variants (A, B, E and F) of KRTAP6-1, and nine genotypes (AA, AB, AE, AF, BB, BE, BF, EE and FF) in these ewes. Among the 243 ewes that had genotypes with a frequency of over 5 % (i.e. AA, AB and BB), the presence of A was found to be associated with reduced mean fibre diameter (MFD) and increased coefficient of variation in fibre diameter (CVFD), whereas the presence of B had a trend of association with decreased coarse edge measurement (CEM). A genotype effect was also detected for MFD and CVFD. No associations were detected for fibre diameter standard deviation (FDSD), mean fibre curvature (MFC) and medulation. These results suggest that variation in KRTAP6-1 affects wool fibre diameter in NZ Romney ewes, confirming the finding in Merino cross-breed sheep.

Variation in ovine KRTAP8-1 is associated with variation in wool fibre staple strength and curvature

2019 ◽  
Vol 157 (6) ◽  
pp. 550-554 ◽  
Author(s):  
H. Gong ◽  
H. Zhou ◽  
W. Li ◽  
J. Wang ◽  
S. Li ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Marker ◽  
Protein Gene ◽  
Wool Fibre ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain ◽  
Staple Strength

AbstractKRTAP8-1 was the initial high-glycine-tyrosine keratin-associated protein gene recognized in sheep, but little is known about the functional influence of this gene. The current study used polymerase chain reaction-single stranded conformational polymorphism analysis to genotype KRTAP8-1 in 391 Southdown × Merino-cross sheep from six sire-lines. Five previously described variants (named A to E) of KRTAP8-1 were identified with frequencies of 67.0, 14.2, 7.0, 10.7 and 1.0%, respectively. Of the four variants (A, B, C and D) that occurred at a frequency greater than 5%, the presence of C was found to be associated with a reduction in mean fibre curvature (MFC) and the presence of D was associated with an increase in mean staple strength (MSS), whereas the presence of A had a trend of association with reduced MSS. Associations were not identified with other wool traits. These results suggest that variation in KRTAP8-1 affects MSS and MFC, and that KRTAP8-1 has the potential to be used as a genetic marker for improving these traits.

Variation in the ovine keratin-associated protein 15-1 gene affects wool yield

2018 ◽  
Vol 156 (7) ◽  
pp. 922-928 ◽  
Author(s):  
W. Li ◽  
H. Gong ◽  
H. Zhou ◽  
J. Wang ◽  
X. Liu ◽  
...  
Keyword(s):  
Fibre Diameter ◽  
Nucleotide Polymorphisms ◽  
Coding Region ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Banding Patterns ◽  
Single Stranded Conformational Polymorphism ◽  
Associated Proteins ◽  
Polymerase Chain ◽  
Wool Yield

AbstractKeratin-associated proteins (KAPs) are constituents of wool and hair fibres and are believed to play an important role in determining the characteristics of the fibres. In the current study, a polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) approach was used to screen for variation in the ovine KAP15-1 gene (KRTAP15-1). Four PCR-SSCP banding patterns, representing four different variants (named A to D), were detected. Four single nucleotide polymorphisms were found within the coding region and three of these were non-synonymous. The effect of this genetic variation on wool traits was investigated in 396 Merino × Southdown-cross sheep. Of the three variants found in these sheep (A, B and C), the presence of B was found to be associated with decreased wool yield, while C was associated with increased wool yield and decreased fibre diameter standard deviation. Sheep of genotype AC had a higher wool yield than those of genotype AA or AB.

scholarly journals Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter

2019 ◽  
Vol 62 (1) ◽  
pp. 125-133 ◽  
Author(s):  
Mengli Zhao ◽  
Huitong Zhou ◽  
Jon G. H. Hickford ◽  
Hua Gong ◽  
Jiqing Wang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Fibre Diameter ◽  
Kidney Tissue ◽  
Single Strand Conformation Polymorphism ◽  
Hair Follicles ◽  
Pcr Analysis ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Association Analyses ◽  
Polymerase Chain

Abstract. Keratin-associated proteins (KAPs) are a structural component of cashmere fibre, and variation in some KAP genes (KRTAPs) has been associated with a number of caprine fibre traits. In this study, we report the identification of KRTAP15-1 in goats. Sequence variation in the gene was detected using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) technique in 250 Longdong goats, and six variants (named A to F) containing eight single nucleotide polymorphisms (SNPs) were identified. Five of the SNPs were non-synonymous and would lead to putative amino acid changes. Reverse-transcription polymerase chain reaction (RT-PCR) analysis revealed that KRTAP15-1 was expressed in secondary hair follicles but not in heart tissue, liver tissue, lung tissue, kidney tissue or the longissimus dorsi muscle. Despite being rich in cysteine, the caprine KAP15-1 protein possesses a high content of serine and moderate content of glycine and phenylalanine. Association analyses revealed that KRTAP15-1 variant A was associated with decreased mean fibre diameter (MFD), and this effect appeared to be dominant; while variant C was found to be associated with increased MFD, the effect being recessive. The findings suggest that caprine KRTAP15-1 is highly polymorphic and that variation in this gene affects cashmere MFD.

scholarly journals Simplified polymerase chain reaction (PCR)-based sexing assists conservation of an endangered owl, the Norfolk Island Boobook Ninox novaeseelandiae undulata

1997 ◽  
Vol 7 (3) ◽  
pp. 283-286 ◽  
Author(s):  
Michael Double ◽  
Penny Olsen
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Second Generation ◽  
Molecular Method ◽  
Chain Reaction ◽  
Norfolk Island ◽  
In The Wild ◽  
Polymerase Chain ◽  
Recovery Effort ◽  
Main Factor

In 1986 a single Norfolk Island Boobook Owl Ninox novaeseelandiae undulata remained. As part of a re-establishment programme, two male New Zealand Moreporks N. n. novaeseelandiae were introduced, one of which survived to pair with the female in the wild and breed successfully. By 1995 the population numbered 12 or 13 individuals of which seven were second generation (F2). However, there were only two breeding pairs. As the 11 hybrids could not be sexed using morphometrics we developed a molecular method based on a recently described avian polymerase chain reaction (PCR)-based sexing technique. The population was found to contain six females and five males. A scarcity of mature males was established as the main factor slowing the recovery effort.

Nucleotide variation in the ovine KRT31 promoter region and its association with variation in wool traits in Merino-cross lambs

2019 ◽  
Vol 157 (2) ◽  
pp. 182-188
Author(s):  
W. Chai ◽  
H. Zhou ◽  
H. Gong ◽  
J. Wang ◽  
Y. Luo ◽  
...  
Keyword(s):  
Promoter Region ◽  
Wool Fibre ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Banding Patterns ◽  
Keratin Gene ◽  
Single Stranded Conformational Polymorphism ◽  
Exon 1 ◽  
Polymerase Chain ◽  
Fleece Weight

AbstractKeratins are the main structural proteins of wool fibres, and it is thought that variation in the keratins may affect wool fibre characteristics. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analyses were used to investigate four regions of the ovine keratin gene KRT31 including a portion of the promoter, the exon 1, exon 3 and exon 7 regions. Initially, in a screening panel of 300 New Zealand Romney, Merino and White Dorper sheep obtained from 26 farms, three, two, two and two PCR-SSCP banding patterns were observed for these four regions, respectively. The promoter region, the exon 1 and exon 3 regions contained two single nucleotide polymorphisms (SNPs) and the exon 7 region contained one SNP. The effect of the variation found in the promoter region on wool traits was subsequently investigated in 485 Southdown × Merino-cross lambs from seven sire-lines. The three variants identified in the original 300 sheep (named A, B and C) were observed with frequencies of 56, 29 and 15%, respectively. The presence of A and B had no significant effect on wool traits, but the presence of C was found to be associated with an increase in greasy fleece weight (GFW), clean fleece weight (CFW) and mean staple length (MSL). There was an effect of genotype on CFW and MSL, with BC sheep producing wool of higher CFW and MSL than AA, AB, AC and BB sheep. These results suggest that ovine KRT31 might be a useful candidate gene for improving wool traits.

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