scholarly journals Taking nature into lab: biomineralization by heavy metal-resistant streptomycetes in soil

2013 ◽  
Vol 10 (6) ◽  
pp. 3605-3614 ◽  
Author(s):  
E. Schütze ◽  
A. Weist ◽  
M. Klose ◽  
T. Wach ◽  
M. Schumann ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Control Soil ◽  
Natural Conditions ◽  
Laboratory Methods ◽  
Dominant Property ◽  
Resistant Strains ◽  
Heavy Metal Resistant ◽  
And Control

Abstract. Biomineralization by heavy metal-resistant streptomycetes was tested to evaluate the potential influence on metal mobilities in soil. Thus, we designed an experiment adopting conditions from classical laboratory methods to natural conditions prevailing in metal-rich soils with media spiked with heavy metals, soil agar, and nutrient-enriched or unamended soil incubated with the bacteria. As a result, all strains were able to form struvite minerals (MgNH4PO4• 6H2O) on tryptic soy broth (TSB)-media supplemented with AlCl3, MnCl2 and CuSO4, as well as on soil agar. Some strains additionally formed struvite on nutrient-enriched contaminated and control soil, as well as on metal contaminated soil without addition of media components. In contrast, switzerite (Mn3(PO4)2• 7H2O) was exclusively formed on minimal media spiked with MnCl2 by four heavy metal-resistant strains, and on nutrient-enriched control soil by one strain. Hydrated nickel hydrogen phosphate was only crystallized on complex media supplemented with NiSO4 by most strains. Thus, mineralization is a dominant property of streptomycetes, with different processes likely to occur under laboratory conditions and sub-natural to natural conditions. This new understanding might have implications for our understanding of biological metal resistance mechanisms. We assume that biogeochemical cycles, nutrient storage and metal resistance might be affected by formation and re-solubilization of minerals like struvite in soil at microscale.

scholarly journals Taking nature into lab: biomineralization by heavy metal resistant streptomycetes in soil

2013 ◽  
Vol 10 (2) ◽  
pp. 2345-2375 ◽  
Author(s):  
E. Schütze ◽  
A. Weist ◽  
M. Klose ◽  
T. Wach ◽  
M. Schumann ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Control Soil ◽  
Natural Conditions ◽  
Laboratory Methods ◽  
Dominant Property ◽  
Resistant Strains ◽  
Heavy Metal Resistant ◽  
And Control

Abstract. Biomineralization by heavy metal resistant streptomycetes was tested to evaluate the potential influence on metal mobilities in soil. Thus, we designed an experiment adopting conditions from classical laboratory methods to natural conditions prevailing in metal-rich soils with media spiked with heavy metals, soil agar, and nutrient enriched or unamended soil incubated with the bacteria. As a result, all strains were able to form struvite minerals on tryptic soy broth (TSB) media supplemented with AlCl2, MnCl2 and CuSO4, as well as on soil agar. Some strains additionally formed struvite on nutrient enriched contaminated and control soil, as well as on metal contaminated soil without addition of media components. In contrast, switzerite was exclusively formed on minimal media spiked with MnCl2 by four heavy metal resistant strains, and on nutrient enriched control soil by one strain. Hydrated nickel hydrogen phosphate was only crystallized on complex media supplemented with NiSO4 by most strains. Thus, mineralization is a~dominant property of streptomycetes, with different processes likely to occur under laboratory conditions and sub-natural to natural conditions. This new understanding may be transferred to formation of minerals in rock and sediment evolution, to ore deposit formation, and also might have implications for our understanding of biological metal resistance mechanisms. We assume that biogeochemical cycles, nutrient storage and metal resistance might be affected by formation and re-solubilization of minerals like struvite in soil at microscale.

scholarly journals Composition and Niche-Specific Characteristics of Microbial Consortia Colonizing Marsberg Copper Mine in the Rhenish Massif

2021 ◽  
Author(s):  
Sania Arif ◽  
Heiko Nacke ◽  
Elias Schliekmann ◽  
Andreas Reimer ◽  
Gernot Arp ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Microbial Consortia ◽  
Rhenish Massif ◽  
Copper Mine ◽  
Alum Shale ◽  
Heavy Metal Resistant ◽  
Detoxification Pathways

Abstract. The Kilianstollen Marsberg (Rhenish Massif, Germany) has been extensively mined for copper ores, dating from Early Medieval Period till 1945. The exposed organic-rich alum shale rocks influenced by the diverse mine drainages at an ambient temperature of 10 °C could naturally enrich biogeochemically distinct heavy metal resistant microbiota. This metagenomic study evaluates the microbially colonized subterranean rocks of the abandoned copper mine Kilianstollen to characterize the colonization patterns and biogeochemical pathways of individual microbial groups. Under the selective pressure of the heavy metal contaminated environment at illuminated sites, Chloroflexi (Ktedonobacteria) and Cyanobacteria (Oxyphotobacteria) build up whitish-greenish biofilms. In contrast, Proteobacteria, Firmicutes and Actinobacteria dominate rocks around the uncontaminated spring water streams. The metagenomic analysis revealed that the heavy metal resistant microbiome was evidently involved in redox cycling of transition metals (Cu, Zn, Co, Ni, Mn, Fe, Cd, Hg). No deposition of metals or minerals, though, was observed by transmission electron microscopy in Ktedonobacteria biofilms which may be indicative for the presence of different detoxification pathways. The underlying heavy metal resistance mechanisms, as revealed by analysis of metagenome-assembled genomes, were mainly attributed to transition metal efflux pumps, redox enzymes, volatilization of Hg0, methylated intermediates of As(III) and reactive oxygen species detoxification pathways.

scholarly journals Antibiotic and Heavy Metal Resistant Bacteria Isolated from Aegean Sea Water and Sediment in Güllük Bay, Turkey : Quantifying the resistance of identified bacteria species with potential for environmental remediation applications

2020 ◽  
Vol 64 (4) ◽  
pp. 507-525 ◽  
Author(s):  
Gülşen Altuğ ◽  
Mine Çardak ◽  
Pelin Saliha Çiftçi Türetken ◽  
Samet Kalkan ◽  
Sevan Gürün
Keyword(s):  
Heavy Metal ◽  
Aegean Sea ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Heavy Metal Resistant ◽  
Seawater Samples

Heavy metal and antibiotic-resistant bacteria have potential for environmental bioremediation applications. Resistant bacteria were investigated in sediment and seawater samples taken from the Aegean Sea, Turkey, between 2011 and 2013. Bioindicator bacteria in seawater samples were tested using the membrane filtration technique. The spread plate technique and VITEK® 2 Compact 30 micro identification system were used for heterotrophic aerobic bacteria in the samples. The minimum inhibition concentration method was used for heavy metal-resistant bacteria. Antibiotic-resistant bacteria were tested using the disk diffusion method. All bacteria isolated from sediment samples showed 100% resistance to rifampicin, sulfonamide, tetracycline and ampicillin. 98% of isolates were resistant against nitrofurantoin and oxytetracycline. Higher antibiotic and heavy metal resistance was recorded in bacteria isolated from sediment than seawater samples. The highest levels of bacterial metal resistance were recorded against copper (58.3%), zinc (33.8%), lead (32.1%), chromium (31%) and iron (25.2%). The results show that antibiotic and heavy metal resistance in bacteria from sediment and seawater can be observed as responses to environmental influences including pollution in marine areas.

Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils

Geochemistry ◽  
2005 ◽  
Vol 65 ◽  
pp. 131-144 ◽  
Author(s):  
Andre Schmidt ◽  
Götz Haferburg ◽  
Manuel Sineriz ◽  
Dirk Merten ◽  
Georg Büchel ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance

scholarly journals Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

2016 ◽  
Vol 75 (s2) ◽  
Author(s):  
Andrea Di Cesare ◽  
Ester Eckert ◽  
Gianluca Corno
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Resistant Bacteria ◽  
Human Pathogens ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Selection Of

<p class="p1">Antibiotic resistant bacteria are found in most environments, especially in highly anthropized waters. A direct correlation between human activities (<em><span class="s1">e.g., </span></em>pollution) and spread and persistence of antibiotic resistant bacteria (ARB) and resistance genes (ARGs) within the resident bacterial communities appears more and more obvious. Furthermore, the threat posed for human health by the presence of ARB and ARGs in these environments is enhanced by the risk of horizontal gene transfer of resistance genes to human pathogens. Although the knowledge on the spread of antibiotic resistances in waters is increasing, the understanding of the driving factors determining the selection for antibiotic resistance in the environment is still scarce. Antibiotic pollution is generally coupled with contamination by heavy metals (HMs) and other chemicals, which can also promote the development of resistance mechanisms, often through co-selecting for multiple resistances. The co-selection of heavy metal resistance genes and ARGs in waters, sediments, and soils, increases the complexity of the ecological role of ARGs, and reduces the effectiveness of control actions. In this mini-review we present the state-of-the-art of the research on antibiotic- and HM-resistance and their connection in the environment, with a focus on HM pollution and aquatic environments. We review the spread and the persistence of HMs and/or ARB, and how it influences their respective gene co-selection. In the last chapter, we propose Lake Orta, a system characterized by an intensive HM pollution followed by a successful restoration of the chemistry of the water column, as a study-site to evaluate the spread and selection of HMs and antibiotic resistances in heavily disturbed environments.</p>

scholarly journals Microbial bioremediation of heavy metals

2021 ◽  
Vol 75 (2) ◽  
pp. 103-115
Author(s):  
Ana Volaric ◽  
Zorica Svircev ◽  
Dragana Tamindzija ◽  
Dragan Radnovic
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Pilot Scale ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Novel Technologies ◽  
Living Organisms ◽  
Microbial Bioremediation ◽  
Physical And Chemical

Heavy metal pollution is one of the most serious environmental problems, due to metal ions persistence, bioavailability, and toxicity. There are many conventional physical and chemical techniques traditionally used for environmental clean-up. Due to several drawbacks regarding these methods, the use of living organisms, or bioremediation, is becoming more prevalent. Biotechnological application of microorganisms is already successfully implemented and is in constant development, with many microbial strains successfully removing heavy metals. This paper provides an overview of the main heavy metal characteristics and describes the interactions with microorganisms. Key heavy metal resistance mechanisms in microorganisms are described, as well as the main principles and types of heavy metal bioremediation methods, with details on successful pilot scale bioreactor studies. Special attention should be given to indigenous bacteria isolated from the polluted environments since such species are already adapted to contamination and possess resistance mechanisms. Utilization of bacterial biofilms or consortia could be advantageous due to higher resistance and a combination of several metabolic pathways, and thus, the possibility to remove several heavy metals simultaneously. Novel technologies covered in this review, such as nanotechnology, genetic engineering, and metagenomics, are being introduced to the field of bioremediation in order to improve the process. To conclude, bioremediation is a potentially powerful solution for cleaning the environment.

scholarly journals Antibiotic Profiling of Heavy Metal Resistant Bacterial Isolates from the Effluent of a Garment Industry in Lalitpur, Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 203-206 ◽  
Author(s):  
M. Sharma ◽  
H.P. Thapaliya
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Heavy Metal Resistance ◽  
Garment Industry ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Bacterial Isolates ◽  
Heavy Metal Resistant ◽  
Multiple Antibiotics

Heavy metal resistant bacterial isolates from the effluent in a garment industry site were examined to assess their resistance towards multiple antibiotics. Heavy metal resistance property has been found to enhance the antibiotic resistance ability of microorganisms. Isolation of the heavy metal resistant organisms was done in media containing salts of heavy metals. Organisms were identified belonging to the genera Bacillus, Corynebacterium, Lactobacillus, Aeromonas and Enterococcus. Bacterial isolates were tested for their sensitivity to seven common antibiotics (penicillin, tetracycline, erythromycin, chloramphenicol, gentamicin, vancomycin and cotrimoxazole) using Kirby-Bauer technique. Isolates were found to be resistant to multiple antibiotics but all the isolates were sensitive to gentamicin. The data of our study indicates that metal pollution of the environment is the cause of heavy metal resistance isolates and hence antibiotic resistance.Key words: Heavy metal, effluent, antibiotics, resistance, Bacteria, pollution.DOI: 10.3126/on.v7i1.2572Our Nature (2009) 7:203-206  

Heavy metal resistant strains are widespread along Streptomyces phylogeny

2013 ◽  
Vol 66 (3) ◽  
pp. 1083-1088 ◽  
Author(s):  
Analía Álvarez ◽  
Santiago A. Catalano ◽  
María Julia Amoroso
Keyword(s):  
Heavy Metal ◽  
Resistant Strains ◽  
Heavy Metal Resistant

scholarly journals Draft Genome Sequence of Heavy Metal-Resistant Soil Bacterium Serratia marcescens S2I7, Which Has the Ability To Degrade Polyaromatic Hydrocarbons

2017 ◽  
Vol 5 (48) ◽  
Author(s):  
Rhitu Kotoky ◽  
L. Paikhomba Singha ◽  
Piyush Pandey
Keyword(s):  
Heavy Metal ◽  
Serratia Marcescens ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Circular Chromosome ◽  
Degrading Bacterium ◽  
Heavy Metal Resistant

ABSTRACT Serratia marcescens S2I7 is a heavy metal-resistant, polyaromatic hydrocarbon-degrading bacterium isolated from petroleum-contaminated sites. The genome contains one circular chromosome (5,241,555 bp; GC content 60.1%) with 4,533 coding sequences. The draft genome sequence includes specific genetic elements for degradation of hydrocarbons and for heavy metal resistance.

Heavy-metal resistance mechanisms developed by bacteria from Lerma–Chapala basin

2021 ◽  
Author(s):  
Ivan Arroyo-Herrera ◽  
Brenda Román-Ponce ◽  
Ana Laura Reséndiz-Martínez ◽  
Paulina Estrada-de los Santos ◽  
En Tao Wang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance
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